• 영어어문교육
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• 제8권2호
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• pp.197-218
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• 2003

The purpose of this study is to help the students in middle school improve their English listening comprehension by presenting effective teaching and learning techniques using animation. A good animation provides a self-contained world with language expressed in a virtual context. A few animation such as "Mulan", "The Emperor's New Clothes", and "Gulliver's Travel" are presented. The materials are primarily for English listening comprehension, enhance awareness of American culture and life-styles, and to encourage students' active role in learning English. It is suggested that their use with content-based instruction, where animation provides relevant schema background, makes language relevant and comprehensible. Practical aspects of classroom instruction are discussed, focusing on the adaption of pre-viewing, while-viewing, and post-viewing activities to the selected animation. It is concluded that careful animation selection, purposeful lesson planning, and the integration of pre-viewing, while-viewing, and post-viewing activities into the content-based lesson encourage natural language skills, especially the listening comprehension and students' interest in English.

• 아동학회지
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• 제21권2호
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• pp.17-31
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• 2000

The purpose of this study was to identify needs of mothers in order to lay a foundation for improving the content of parent education in early childhood educational institutions. The mothers of 164 kindergarten children in Pusan participated in this study by a questionnaire developed by the researchers. Conclusions were that the felt needs of mothers identified in this study should be included in parent education programs along with knowledge, skills and attitudes selected by professionals. Selection of content should be based on the fact that most mothers have similar concerns and interest in raising their children, regardless of background. However, mother's educational level needs to be considered. Further study needs to be done on educational methods, and tentative models of parent education need to be designed in order to activate effective parent education.

• 한국지능시스템학회:학술대회논문집
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• 한국퍼지및지능시스템학회 2002년도 추계학술대회 및 정기총회
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• pp.295-299
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• 2002

This paper presents an image contrast enhancement technique for improving low contrast images. We applied fuzzy logic to develop an image contrast enhancement technique in the viewpoint of considering that the low pictorial information of a low contrast image is due to the vaguness or fuzziness of the multivalued levels of brightness rather than randomness. The fuzzy image contrast enhancement technique consists of three main stages, namely, image fuzzification, modification of membership values, and image defuzzification. In the stage of image fuzzification, we need to select a crossover point. To select the crossover point automatically the K-means algorithm is used. The problem of crossover point selection can be considered as the two-category, object and background, classification problem. The proposed method is applied to an experimental image with 256 gray levels and the result of the proposed method is compared with that of the histogram equalization technique. We used the index of fuzziness as a measure of image quality. The result shows that the proposed method is better than the histogram equalization technique.

• 한국산림과학회지
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• 제96권2호
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• pp.125-130
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• 2007

The principal morphological characters used for identification of hydrangea cultivars are often dependent on agroclimatic conditions. Furthermore, information on the selection or the genetic background of the hydrangea breeding is so rare that a molecular marker system for cultivar identification is needed. Amplified fragment length polymorphism (AFLP) markers were employed for fingerprinting Hydrangea macrophylla cultivars and candidate cultivars of H. macrophylla selected in Korea. One AFLP primer combination was sufficient to distinguish 17 H. macrophylla cultivars and 4 candidate cultivars. The profile of 19 loci that can minimize the error of amplification peak detection was constructed. AFLP markers were efficient for identification, estimation of genetic distances between cultivars, and cultivar discrimination. Based on the observed AFLP markers, genetic relationship was reconstructed by the UPGMA method. Seventeen H. macrophylla cultivars and H. macrophylla for. normalis formed a major cluster, and candidate cultivars selected in Korea formed another cluster.

• 한국문헌정보학회지
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• 제35권2호
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• pp.23-40
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• 2001

디지털 기술은 정보의 생산, 저장, 배포를 위하여 사용된다. 본 연구에서는 기술적, 경제적, 국가적인 관점에서 디지털 아카이브의 현황을 조사하였으며 디지털 정보의 장기 보존과 관련된 배경과 이슈들에 관하여 분석하였다. 또한 본 연구에서는 디지털 아카이브의 선택, 구분, 보존, 관리 등의 구성 요소와 미래의 전망에 관하여 고찰하였다.

• 연구윤리
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• 제5권1호
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• pp.7-11
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• 2024

Purpose: This study seeks to discuss research ethics, not only the academic honesty and sincerity that researchers who study aviation services academically should have, but also the direction of the moral aspects that are fundamentally required as researchers. Additionally, this study seeks to examine the realistic problems of research related to the aviation service industry, a field of social science. Lastly, focusing on research ethics in the aviation service field, we will look at the theoretical background and the problems in the actual research field, and draw implications based on this. Research design, data and methodology: This study conducted an exploratory study through a selection process based on research ethics topics and research ethics related to the aviation service industry. Results: Efforts to systematize research ethics in research areas related to the aviation service industry, which is a field of social science, require efforts to expand the scope of systematization of research ethics related to the aviation service industry by referring to systemization efforts in other academic fields. In addition, specific systemization efforts will be needed through cooperation between universities, research institutes, and academic organizations. Also, concrete systematization efforts will be needed through cooperation between universities and academic organizations.

• Journal of Plant Biotechnology
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• 제46권3호
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• pp.165-171
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• 2019

Marker-assisted backcrossing (MABC)은 marker-assisted selection (MAS)와 함께 다양한 작물에서 여교배 초기세대에서 반복친 게놈의 회복률이 높은 개체선발을 위한 분자육종 기술로 매우 유용하게 사용하고 있다. 본 연구에서는 토마토 MABC 육종 프로그램의 일환으로 저장성이 강한 rin유전자를 주)토마토연구소에서 육성한 핑크계 엘리트 토마토계통에 도입하고자 수행하였다. foreground 선발은 RIN SCAR 분자 마커를 이용하여 100개 $BC_1F_1$ 식물체에서 Rr 유전자형 가진 42개체를 선발하였다. 그리고 이를 이용하여 GBS 분석을 이용하여 background 선발을 하였다. 총 3,086개 SNP를 대상으로 반복친 HK13-1151과 게놈 회복률을 조사한 결과, 56.7%에서 84.5%를 보여 평균 70.5%로 나타났다. 이 중 87.2%을 보인 $BC_1F_1$개체를 이용하여 192개 $BC_2F_1$ 식물체를 육성하여 foreground 선발을 하였다. 선발된 102개 중 88개 식물체를 이용하여 GBS 분석을 수행한 결과 4,868개의 다형 SNP 마커를 얻었으며, 이를 이용하여 RPG 회복률을 조사하였다. $BC_2F_1$ 식물체들에서 HK13-1151 반복친 게놈과 87.8%에서 97.8% 유사하였다. 본 연구에서 $BC_2F_1$ 식물 중 RPG 회복률이 97.8%인 5-1 개체는 반복친인 HK13-1151과 과일특성에서 매우 유사하였다. 따라서 선발된 5-1 개체는 $BC_2F_2$ 세대를 육성하여 계통화 하고자 한다. 본 연구를 통해 MABC는 전통 여교배 육종에 비해 육종연한을 획기적으로 줄일 수 있으며, 원하는 육종모델을 완수할 수 있는 첨단육종 기술로 평가 할 수 있다.

• 대한인간공학회지
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• 제34권3호
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• pp.199-215
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• 2015

Objective: The purpose of this study is to find the level of physical relaxation of individual subject by monitoring psychophysiological biofeedback to different types of mattresses. And, the study also aims to find a protocol to make a selection of the best mattress based on the measured information. Background: In Korea, there are an increasing number of people using western style bed. However, they are often fastidious in choosing the right mattress for them. In fact, people use their past experience with their old mattress as well as the spontaneous experience they encounter in a show room to finally decide to buy a bed. Method: Total five mattresses were tested in this study. After measuring the elasticity of the mattresses, they were sorted into five different classes. Physiological and psychological variables including Electromyography (EMG), heart rates (HR), oxygen saturations (SaO2) were used. In addition, the peak body pressure concentration rate was used to find uncomfortably pressured body part. Finally, the personal factors and subjective satisfaction were also examined. A protocol was made to select the best mattress for individual subject. The selection rule for the protocol considered all the variables tested in this study. Results: The result revealing psychological comfort range of 0.68 to 0.95, dermal comfort range of 3.15 to 6.07, back muscle relaxation range of 0.25 to 1.64 and personal habit range of 2.0 to 3.4 was drawn in this study. Also a regression model was developed to predict biofeedback with the minimal use of biofeedback devices. Moreover results from the proposed protocol with the regression equation and subjective satisfaction were compared with each other for validation. Ten out of twenty subjects recorded the same level of relaxation, and eight subjects showed one-level difference while two subjects showed two-levels difference. Conclusion: The psychophysiological variables and suitability selection process used in this study seem to be used for selecting and assessing ergonomic products mechanically or emotionally. Application: This regression model can be applied to the mattress industry to estimate back muscle relaxation using dermal, psychophysiology and personal habit values.

• IMMUNE NETWORK
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• 제3권3호
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• pp.219-226
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• 2003

Background: Phage display is the most widely used technique among display methods to produce monoclonal antibody fragment with a specific binding activity. Having a large library for efficient antibody display/selection is quite laborious process to have more than $10^9$ members of transformants. To overcome these limitations, several in vitro selection approaches have been reported. Ribosome display that links phenotypes, proteins, directly to genotype, mRNA, is one of the in vitro display methods. Ribosome display can reach the size of scFv library up to $10^{14}$ molecules and it can be further diversified during PCR steps. To select the high affinity scFv from one pot library, we established ribosome display technique by modifying the previously reported eukaryotic translation system. Methods: To establish the antibody selection system by ribosome display, we used 3D8, anti-DNA antibody. A 3D8 scFv was synthesized in vitro by an in vitro transcription-translation system. The translated 3D8 scFv and the encoding 3D8 mRNA are connected to the ribosome. These scFv-ribosome-mRNA complexes were selected by binding to their specific antigens. The eluted mRNAs from the complexes are reverse transcribed and re-amplified by PCR. To apply this system, antibody library from immunized mouse with terminal protein (TP)-peptide of hepatitis B virus DNA polymerase TP domain was also used. This TP-peptide encompasses the 57~80 amino acid residues of TP. These mRNA/ribosome/scFv complexes by our system were panned three times against TP-peptide. The enrichment of antibody from library was determined by radioimmunoassay. Results: We specifically selected 3D8, anti-DNA antibody, against ssDNA as a model system. The selected 3D8 RNAs sequences from translation complexes were recovered by RT-PCR. By applying this model system, we enriched TP-peptide-specific scFv pools through three cycles of panning from immunized library. Conclusion: We show that our translating ribosome complexes are well maintained and we can enrich the TP-specific scFv pools. This system can be applied to select specific antibody from an antibody library.

• 한국조리학회지
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• 제21권1호
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• pp.250-266
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• 2015

오늘날 실버세대는 독립된 소비세대로 사회 여러 분야에서 실버외식산업을 급성장시키는 세대이기도 하다. 본 연구에서는 새로운 소비의 주체세력으로 부상하고 있는 실버세대를 대상으로 실버세대에 대한 외식 소비행동에 따른 외식 선택속성과 식습관 변화를 살펴보고자 하였다. 서울에 거주하는 55세 이상의 실버 연령인 216명을 대상으로 하여 식습관 수준과 인구사회학적 특성에 따른 변인분석은 독립표본 t-검증과 F-검증을 실시하였으며, 실버세대의 식습관에 따른 소비태도와 외식소비행동 차이를 알아보기 위하여는 ${\chi}^2$검증을 실시하였다. 분석한 연구 결과를 요약하여 다음과 같은 결과를 얻었다. 첫째, 실버세대의 식습관 수준은 3점 만점에 평균 2.02점으로 나타나, 식습관이 어느 정도 양호함을 알 수 있었다. 둘째, 실버세대의 식습관에 따른 외식소비태도 차이를 분석한 결과, 식습관 태도가 비교적 양호한 사람들이 불량한 집단보다 물리적인 환경, 메뉴의 다양성, 업체의 품질과 서비스를 찾아서 외식업체를 선택하는 것으로 나타났다. 셋째, 실버세대의 식습관에 따른 외식소비행동 변화를 분석한 결과, 식습관 수준에 따른 외식업체 정보 출처와 외식업체 선택 선정기준 변수에 대해서만 집단 간 차이가 있는 것으로 나타났다.