• 한국약용작물학회지
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• 제20권5호
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• pp.339-344
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• 2012

This study was carried out to research microorganisms having the antifungal activity against ginseng Alternaria blight pathogen Alternaria panax and ginseng anthracnose pathogen Colletotrichum gloeosporioides. Eleven Bacillus strains. were isolated from Korean traditional soybean paste and Kimchi. Among the 11 isolates, DJ5, KC1, KC2 and KC4 showing antagonistic activity on the mycelial growth of A. panax and C. gloeosporioides in pairing culture were finally selected as the antagonistic microorganisms. Based on 16s rRNA sequence and phylogenetic tree analysis, they were identified as Bacillus spp.. The selected microorganisms were investigated antagonistic activity by measured leaf-segment colonization in pot test. When Bacillus sp. were injected after A. panax treatment, KC1, KC2 and KC4 showed similar effect to chemical pesticides treated control. To measure preventive effect of Bacillus sp, antagonistic microorganisms were injected and C. gloeosporioides was treated in pot. When measuring the effectiveness for the prevention of Anthracnose, All Bacillus spp. showed approximately 83~90 % degree of superior preventive effect. In general, The four Bacillus spp. isolated from Korean traditional fermented foods showed therapeutic effect of Alternaria blight and preventive effect of Anthracnose.

• 한국식품영양학회지
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• 제26권2호
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• pp.280-286
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• 2013

본 연구에서는 단백질이 풍부한 원료 콩에 청국장에서 분리한 Bacillus subtilis와 김치에서 분리한 Lactobacillus plantarum을 이용한 혼합 발효를 통해 대두 발효 제품의 개발을 목표로 한 연구결과는 다음과 같은 분석 결과를 얻었다. Bacillus subtilis 단독 배양 발효 두유, Lactobacillus plantarum 단독 배양 발효 두유, 또한 두 균을 혼합 배양한 발효 두유의 항산화 활성을 상호 비교하였다. 그 결과, Bacillus subtilis 단독 배양 발효 두유의 총 폴리페놀 함량, DPPH 라디칼 소거능, 환원력, 아질산염 소거능이 각각 3580.91 ${\mu}g/m{\ell}$, 67.14%, 6.54$mg/m{\ell}$, 83.63%로 가장 높았다. 혼합 발효 두유의 기능성을 증진시키기 위하여 토마토 추출물을 첨가하여 발효 두유를 제조하였고, 토마토 에탄올 추출물을 0, 0.25, 0.5, 그리고 1.0% 첨가한 토마토 요구르트의 항산화력은 토마토 첨가량 농도 의존적으로 항산화력이 유의적으로 증가하였다. 특히 DPPH 라디칼 소거능은 토마토 무첨가구는 28.39%인데 비해, 토마토 요구르트는 33.46~57.12%까지 증가하였다.

• Current Research on Agriculture and Life Sciences
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• 제31권1호
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• pp.30-37
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• 2013

Bacillus subtilis and Bacillus amyloliquefaciens are closely related species that share a similar genomic background, and are both known to secrete large amounts of proteins directly into a medium. The extracellular proteomes of two strains of Bacillus subtilis and two strains of Bacillus amyloliquefaciens were compared by 2-D gel electrophoresis during the late exponential growth phase. The relative abundance of some minor protein spots varied among the four strains of Bacillus. Over 123 spots of extracellular proteins were visualized on the gel for B. subtilis CH 97, 68 spots for B. subtilis 3-5, 230 spots for B. amyloliquefaciens CH 51, and 60 spotsfor B. amyloliquefaciens 86-1. 2D gel electrophoresis images of the four Bacillus strains showed significantly different protein profiles. Consistent with the 2D gel electrophoretic analysis, most of the B. subtilis proteins differed from the proteases secreted by the B. amyloliquefaciensstrains. Among the proteins identified from B. subtilis, approximately 50% were cytoplasmic and 30% were canonically extracellular proteins. The secreted protein profiles for B. subtilis CH 97 and B. subtilis 3-5 were quite different, as were the profiles for B. amyloliquefaciens CH 51 and 86-1. The four proteomes also differed in the major protein composition. The B. subtilis CH 97 and B. amyloliquefaciens CH 51 proteomes both contained large amounts of secreted hydrolytic enzymes. Among the four strains, B. subtilis 3-5 secreted the least number of proteins. Therefore, even closely related bacteria in terms of genomic sequences can still have significant differences in their physiology and proteome layout.

• 한국토양환경학회지
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• 제4권2호
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• pp.33-43
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• 1999

흡착제 생산균주를 선별하기 위하여 토양시료에서 분리한 홉착제 생산균주중 가장 뛰어난 흡착물질을 생산하는 균주, SK3l을 선별하였으며 분리균주 SK3l은 Bacilus속으로 동정되었다. Bacillus sp. SK3l에 의하여 생산된 흡착물질(생물흡착제 SK3l)은 ethanol 침전과 cetylpyridinium(CPC)침전을 통하여 정제하여 아연과 납 이온에 대한 흡착특성을 조사하였다. 생물흡착제 SK3l의 아연과 납 이온의 흡착량은 각각 52 mg/g과 112 mg/g이었다. Bacillus sp. SK3l 균주가 흡착제를 생산하기위한 flask수준에서의 배양조건들이 조사되었다. 흡착제 생산을 위한 최적 pH와 최적온도는 각각 7.5와 $30^{\circ}C$로 나타났다. 흡착제 생산시 주요한 탄소원과 질소원은 glucose와 ammonium nitrate이었다. 최적화된 배지에서의 흡착제 생산은 기초배지에서 보다 약 3배 증가하였다. Jar fermentor배양에서 배양 60시간에서 가장 많은 흡착제를 생산하였으며, 흡착제의 생산량은 9.2 g/$\ell$이었다.

• 한국식품저장유통학회지
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• 제10권2호
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• pp.224-229
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• 2003

양파 근권미생물은 양파 뿌리를 균원시료로 하여 약 250여종의 근권미생물을 순수 분리하였고 이들 중에서 길항미생물은 무름병균인 Erwinia carotovora subsp. carotovora와 시들음병균인 Fuarium oxysporium에 각각 서로 대치 배양하여 두 병원균 모두에 가장 강한 길항 효과를 나타내는 YJ-4 균주를 최종 선발하였다. 선발균주 YJ-4는 Bergey's mannual of systematic bacteriology 방법에 따라 조사하여 Bacillus 속으로 판정하였고, 세포벽 지방산을 분석하여 동정하는 Sherlock system에 의해 Bacillus ehimensis와 0.778의 유의성을 확인하였으며, 165 rDNA의 부분 염기서열(609bp)로 Bacillus ehimensis와 98.36%의 높은 유사도를 보여 길항균 YJ-4는 Bacillus ehimensis로 동정하였다. B. ehimensis YJ-4로 명명한길항균은 세균인 무름병균 (Erwinia carotovora subsp. carotovora)과 세균썩음병균 (Pseudomonas sp.)에도 길항력을 나타내었고, 진균인 잘록병균 (Rhizoctonia solani), 잎마름병균 (Stemphylium botryosum), 검은무의병균 (Alternaria porri), 검은점잎마름병균 (Septoria sp.), 잿빛곰팡이병균 (Botrytis cinerea), 흑색썩음균핵병균 (Sclerotium cepivotum), 시들음병균 (Fusarium oxysporium), 푸른곰팡이병균 (Penicillium sp.)등에도 길항 효과를 나타내었다. 특히 시들음병균인 F. oxysporium에 생육억제환의 길이가 24.8mm로 가장 높은 길항력을 나타내었다.

• Journal of Species Research
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• 제7권3호
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• pp.202-209
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• 2018

Eight bacterial strains, 18JY8-13, 18JY13-16, 18JY43-7, 18JY12-7, 18JY1-1, 18JY1-7, 18JY15-3, and 18JY7-2 assigned to the phylum Firmicutes were isolated from a variety of soil samples collected in the Jeju Island, Korea. Cells of the eight strains were Gram-positive, aerobic and showed resistant to UV-radiation. Phylogenetic analysis based on 16S rRNA gene sequence revealed that strains 18JY8-13, 18JY13-16, 18JY43-7, 18JY12-7, 18JY1-1, 18JY1-7, 18JY15-3, and 18JY7-2 were most closely related to Bacillus paranthracis(99.9%), Bacillus paramycoides(99.6%), Bacillus australimaris(99.9%), Bacillus wiedmannii (100%), Bacillus halosaccharovorans(99.6%), Bacillus deserti(98.7%), Bacillus cereus (99.8%), and Bacillus albus(100%), respectively. This is the first report of these eight species in Korea.

• 한국식품과학회지
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• 제28권1호
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• pp.66-71
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• 1996

쌀밥의 저장성을 향상시킬 목적으로 쌀밥 부패 미생물을 분리, 동정하고 이들과 표준 균주에 대한 녹차 물추출물의 항균활성을 측정하였다. 표준균주 Bacillus subtilis ATCC 6633, Salmonella typhimurium ATCC 14028, Bacillus cereus YUFE 2004 및 Staphylococcus aureus YUFE 2087 모두 녹차 물추출물이 500, 1000 ppm 첨가된 broth system에서 생육이 억제되었다. 쌀밥 부패에 관여하는 주요 미생물은 두 균주 모두에서 형태적, 생리적 특성과 API 50 CHB kit 그리고 균체 지방산 분석을 통해 Bacillus subtilis로 동정되었다. 분리, 동정된 균주 중 Bacillus subtilis RHJ-I의 생육은 녹차 물추출물이 500, 1000 ppm 첨가된 broth system에서 그 생육이 억제되었다.

• Journal of Biosystems Engineering
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• 제43권4호
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• pp.394-400
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• 2018

Purpose: This research evaluated five types of nanoparticles to develop a surface-enhanced Raman spectroscopy (SERS) method for the rapid detection of two Bacillus species (Bacillus cereus and Bacillus thuringiensis) that are commonly found on fresh produce, which can cause food poisoning. Methods: Bacterial concentrations were adjusted to a constant turbidity, and a total of $30{\mu}L$ of each Bacillus cell suspension was prepared for each nanoparticle. A point-scan Raman system with laser light source of wavelength 785 nm was used to obtain SERS data. Results: There was no qualitative difference in the SERS data of B. cereus and B. thuringiensis for any of the five nanoparticles. Three gold nanoparticles, stabilized in either citrate buffer or ethanol, showed subtle differences in Raman intensities of two Bacillus species at $877.7cm^{-1}$. Conclusions: Among the three types of nanoparticles, the gold nanoparticles stabilized in citrate buffer showed the lowest standard deviation, followed by gold nanoparticles stabilized in ethanol. This result supports the potential application of gold nanoparticles for SERS-based detection of B. cereus and B. thuringiensis.

• Animal Bioscience
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• 제37권6호
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• pp.1041-1052
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• 2024

Objective: Bacillus subtilis, a kind of probiotic with broad-spectrum antibacterial function, was commonly used in livestock and poultry production. Recent research suggested that Bacillus subtilis may have antioxidant properties and improve immune response. This study aimed to verify the probiotic function of Bacillus subtilis in the production of broiler chickens. Methods: A total of 324 (1-day-old) Arbor Acres broilers were selected and randomly divided into three groups: basal diet group (Ctr Group), basal diet + antibiotic growth promoter group (Ctr + AGP) and basal diet + 0.5% Bacillus subtilis preparation group (Ctr + Bac). The experiment lasted for 42 days. Muscle, serum and liver samples were collected at 42 days for determination. Results: The results showed that Bacillus subtilis could decrease malondialdehyde content in the serum and liver (p<0.05) and increase superoxide dismutase 1 mRNA expression (p<0.01) and total superoxide dismutase (p<0.05) in the liver. In addition, compared with AGP supplementation, Bacillus subtilis supplementation increased interleukin-10 (IL-10) and decreased tumor necrosis factor-α and IL-1β level in the serum (p<0.05). At 45 minutes after slaughter Ctr + Bac presented a higher a^* value of breast muscle than Ctr Group (p<0.05), while significant change in leg muscle was not identified. Moreover, there was no difference in weight, shear force, cooking loss and drip loss of breast and leg muscle between treatments. Conclusion: Our results demonstrate that Bacillus subtilis in diet can enhance antioxidant capacity and optimize immune response of broilers.

• 한국식품영양과학회지
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• 제23권6호
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• pp.980-985
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• 1994

We investigated the quality of soybean paste(Doenjang) fermented by BAcillus brevis. The results obtained were as follows : soybean paste fermented by Bacillus brevis had alkaline pH and yellow ochre color. Dextrinizing activity was about 98 D.P. unit from 5th to 25th day of fermentation at 3$0^{\circ}C$ and after that day somewhat decreased . Saccharifying activity was respectively 6.1, 7.2, 6.8, 6.4 S.P. unit on 5, 15, 25 and 35th day of fermentation. Protease activity suddenly increased after 15th day of fermentation and was 250, 275, 299 unit on15, 25, 35 th day of fermentation , respectively. The most abundant free amino acid was found to be glutamic acid (561.8mg%) in soybean paste fermented by Bacillus brevis. In case of free sugar and non-volatile organic acid, fructose and oxalic acid showed highest content of 10.25mg% on 25th day and 12.20mg% on 15th day. The contents of free amino acids, free sugars, organic acids in soybean paste fermented by B.brevis were most abundant after 25 days of fermentation and this results were similar to that of traditional soybean paste. The odor of soybean paste fermented by Bacillus brevis was improved to be a nice soybean paste odor on 25th day of fermentation. However, sensory evaluation value of the taste of it decreased after 10 th day of fermentation.