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Application of Biosynthesized Silver Nanoparticles Against a Cancer Promoter Cyanobacterium, Microcystis aeruginosa

  • El-Sheekh, Mostafa Mohamed;El-Kassas, Hala Yassin
    • Asian Pacific Journal of Cancer Prevention
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    • 제15권16호
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    • pp.6773-6779
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    • 2014
  • Background: Nanotechnology opens new applications in many fields including medicine. Among all metallic nanoparticles, silver nanoparticles (silver NPS) have proved to be the most effective against a large variety of organisms including toxic cyanobacteria. Materials and Methods: Silver NPs were biosynthesized in vivo with different alga species namely, Spirulina piatensis, Chlorella vulgaris and Scenedesmus oh/iquus following two scenarios. First: by suspending a thoroughly washed algae biomass in 1 mM aqueous $AgN0_3$ solution. Second: by culturing them individually in culture media containing the same concentration of $AgN0_3$. Silver NPs were characterized using UV-Vis spectroscopy, transmission electron microscopy (TEM), energy dispersive analysis (EDX) and Fourier transform infra-red (FfIR) spectroscopy. The biosynthesized silver NPs were tested for cytotoxic activity against a cancer promoter cyanobacteruim Microcystis aeruginosa, considering effects on cell viability and chlorophyll content. Results: The surface plasmon band indicated the biosynthesis of silver NPs at ~400 nm. Transmission electron microscopy (TEM) revealed that the silver NPs had a mean average size below 100 nm. Energy-dispersive analysis X-ray (EDX) spectra confirmed the presence of silver element. FfIR spectral analyses suggested that proteins and or polysaccharides may be responsible for the biosynthesis of silver NPs and (-COO-) of carboxylate ions is responsible for stabilizing them. The toxic potentialities ofthe biosynthesized silver NPs against the cancer promoter cyanobacterium, Microcystis aeruginosa showed high reduction in viable cells count and the total chlorophyll content. Conclusions: The potential activity of the biosynthesized silver NPs from the studied algae species against Microcystis aernginosa cells is expected to be mainly mediated by the release of silver ions (Ag+) from the particle surface and bioactive compounds as indicated by FfIR analysis.

화농성 주관절염의 관절경적 처치 (Arthroscopic Management of Septic Arthritis of the Elbow)

  • 문영래;박준광;오서진
    • 대한관절경학회지
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    • 제3권2호
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    • pp.138-141
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    • 1999
  • 목 적 : 화농성 주관절염의 치료 방식 중의 하나인 관절경적 처치를 제시하고, 경과를 평가하고자 하였다. 대상 및 방법 : 급성 화농성 주관절염 환자 7례를 대상으로 하였으며, 연령 분포는 6세에서 32세로 다양한 양상을 보였다. 관절 천자와 임상 검사에 의해 확진되면 즉시 응급으로 관절경적 변연 절제술 및 세척술을 시행하였고, 환자의 경과 관찰을 시행하였다. 결 과 : 혈액 검사상 백혈구 수치 및 적혈구 침강 속도와 c-반응성 단백의 정상화는 소아에서 8.4일, 성인에서 12.3일 소요되었다. 1년 추시 결과 전례에서 주관절 운동 제한은 없었고, 혈액 검사상 정상 소견을 보였으며, 그 외 후유증이나 합병증은 관찰되지 않았다. 결 론 : 화농성 관절염의 치료는 적절한 배농과 항생제 처치가 원칙이며, 이러한 관점에서 본다면 관절경적 변연 절제술 및 세척술은 다른 방식에 비해 관절 연골을 보호하고 전체적인 병리 상태를 관찰하면서 시행할 수 있는 유용한 방식 중의 하나로 사료된다.

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'노상주점의 위생상태에 관한 미생물학적 조사' ('Survey on Bacteriological Contamination of Moving Tavern in Seoul Area')

  • 유병태
    • 환경위생공학
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    • 제1권1호
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    • pp.59-67
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    • 1986
  • This sanitary survey was carried out to investigate the bacteriological contamination of cooking utensils and foods of moving tavern in eight sample sites of Seoul area. The results of survey were as follows: 1. The counts by means of total bacteria in cooking utensils and food samples by standard plate count method were as follow: $5.6\times10^5$ per gm in dishcloth, $3.1\times10^6$ per ml in dishwater. In food samples, $5.4\times10^5$ per gm in meat was higher than other samples. 2. The average counts total coliform and fecal coliform in samples by MPN method were as follow: $3.4\times10^4$ MPN per 100ml, and $1.3\times10^2$ MPN per 100ml in chopping board, $6.1\times10^4$MPN per gm and $1.0\times10^2$ MPN per gm in dishcloth, $1.8\times10^5$ MPN per 100ml and $6.1\times10^2$ MPN per 100ml in dishwater. In food samples, $3.1\times10^4$MPN per gm and $2.0\times10^2$ MPN per gm in meat was higher than other samples. 3. The counts by means of Pseudomonas in samples by MPN method were as follow: $2.8\times10^3$ MPN per 100ml in chopping board, $4.7\times10^3$ MPN per gm in dishcloth $5.6\times10^3$ MPN per 100ml in dishwater. In food samples, $2.4\times10^3$ MPN per gm in shellfish was higher than other samples. 4. Isolation cases of Food poisoning organisms from samples were as follow: Staphylococci was detected 9 cases $(17.6\%)$ in chopping board, 7 cases $(13.6\%)$ in dishcloth. In food samples, 9 cases $(25.7\%)$ in meat, 1 case $(4\%)$ in fish samples. Salmonella was detected 2 cases $(3.9\%)$ in dishwater, 1 case in meat samples.

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경상남도(慶尙南道) 밀양군(密陽郡) 삼랑진읍(三浪津邑)에 있어서의 간흡충(肝吸虫) 감염실태조사(感染實態調査) (Epidemiological Note on the Clonorchiasis in Samrangjin Eup, Milyang Gun, Kyongsang Nam Do (=province))

  • 임한종;주경환;엄기선;박수배
    • 농촌의학ㆍ지역보건
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    • 제7권1호
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    • pp.80-89
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    • 1982
  • In order to observe the endemic status of Clonrchis sinensis infection in Samrangjin eup, Milyang gun, Kyongsang nam do, a total number of 294 stool specimens were collected from the inhabitants who lived in the basin of the Nakdong river. The specimens were examined by cellophane thick smear technique and Stoll's egg dilution technique during the period from May to June 1983. The epidemiological status was analysed statistically by the regression equation and catalytic curv with the results obtained from this area. The results are as follows : 1) The infestation rate of Clonorchis sinensis in 294 was 49.0%. 2) No remarkable difference was observed between male (52.5%) and female (44.8%). 3) Average E.P.G. by Stoll's egg dilution technique in this area was 9,597. 4) The degree of the intensity of Clonorchis infection by E.P.G. count was distributed as 10.5% in very heavy infection, 14.7% in heavy infection, 47.4% in moderate infection, and 29.3% in light infection. 5) The intensity of endemicity in this area was represented with the regression equation calculated with the cumulative percentages of E.P.G. counts. Regression equation was y=3.40+1.23 log x and Cs. $D._{50}$ was 19.99. 6) The two stage catalytic model was applied and the calculation lead to the equation $y=1296(e^{-0.008t}-e^{-0.035t})$ ; a=0.035 > b=0.008 in this surveyed area. 7) Other helminthic infection rate in this area was 7.1% in A. lumbricoides, 9.5% in T. trichiura and 4.1% in E. vermicularis respectively.

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H.264/AVC를 위한 고성능 CAVLC 부호화기 하드웨어 설계 (Hardware Design of High Performance CAVLC Encoder)

  • 이양복;류광기
    • 대한전자공학회논문지SD
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    • 제49권3호
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    • pp.21-29
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    • 2012
  • 본 논문에서는 H.264/AVC 부호화기의 성능 향상을 위해 고성능 CAVLC 부호화기의 하드웨어 구조를 제안한다. 기존의 CAVLC 부호화기는 변환계수의 재정렬 과정이 포함되어 변환계수를 저장해야 할 버퍼와 버퍼제어를 위한 추가적인 사이클이 필요하므로 하드웨어 면적이 증가하고 불필요한 사이클이 수행된다. 제안한 CAVLC는 CAVLC의 파라미터 중에 Runbefore를 순방향 탐색기법으로 계산하고 그 외 파라미터들은 역방향 탐색기법으로 계산하여 변환계수의 재정렬 과정을 수행하지 않는다. 또한, 제안한 CAVLC 부호화기에 조기 종료 모드를 적용하고 2단 파이프라인 구조를 사용하여 CAVLC의 수행 사이클 수를 감소시켰다. 제안한 CAVLC 부호화기의 하드웨어 구조를 매그나칩 공정 $0.18{\mu}m$ 셀 라이브러리로 합성한 결과, 최대동작 주파수는 125MHz이며 게이트 수는 17k이다. 제안한 CAVLC 부호화기의 하드웨어 구조를 H.264/AVC 표준 참조 소프트웨어 JM13.2에서 추출한 데이터를 이용하여 테스트한 결과, $16{\times}16$ 매크로블록을 처리하는데 평균적으로 36.0사이클이 소요되어 기존의 CAVLC 부호화기보다 성능이 57.8% 향상됨을 확인하였다.

3,4호선 도시철도 전동차 도어엔진의 개발 역사에 관한연구 (A Study on History of Rolling Stock Door Engines of Seoul Metro Line No.3 and No.4)

  • 장성철;이찬희;전관수;손영진
    • 한국철도학회:학술대회논문집
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    • 한국철도학회 2010년도 춘계학술대회 논문집
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    • pp.1352-1359
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    • 2010
  • It is said that the number of people using public transportation has risen about 220 thousand over 3 years which was 1019 people average a day in 2006 and 1041 people last year. It is also said that the number of people using subway has increased by 198 thousand and the number of people using bus by 22 thousand. Can you imagine how many door engines work at the same time if we count Metro line no.1 to no.4 which consist of total 120 subway stations? A train has 80 door engines and Metro line trains have 9600 door engines all together. Which explains it quite simple how much Satefy, Durability and Sustainability need to be focused in Door Engines. Although it's not the whole part of door engines in Seoul Metro Line, And Metro No.4's door engines are operated by the method called mixture of mechanical type and belt type. And the last one is being used in the brand-new Metro train line No.3 which is being operated by electricity motor, instead of the old methods which use air pressure to operate a door engine. I'm sure you will agree that Safety is the first priority of Metro train and next follow comfortability and quickness. I think all I've talked so far make it the first step for the Safety of Metro train for you to understand the unit of a Door Engine.

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핵청진기를 이용한 좌심실 부피변화 측정에 관한 연구 (A Study Left Ventricular Volume Change Measurement using Nuclear Stethoscope)

  • 민병구;김영호;고창순
    • 대한의용생체공학회:의공학회지
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    • 제5권2호
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    • pp.167-172
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    • 1984
  • In the present study, we developed a nuclear stethoscope interfaced with a personal computer (APPLE- II) and evaluated it's performances as compared with the gamma camera. This single-probe, ECG-gated system has been used for displaying the left ventricular time-activity curves, the peak ejection time and its rate, the peak filling time and its rate, and the R-R interval distribution. The radioactivity was measured at every 10msec around the ventricular region, where the activity curves shows the maximal peak to peak variations. The background activity was measured around the lung area showing its counts approximately 50% of the end-diastolic count with minimal variations. The average time-activity curves of 100 beats were used for analysis in the equilibrium study after intravenous injection of 15-20mCi of Tc. The ejection fractions measured by the nuclear stethoscope(Y) were compared with those measured by gamma camera(X) in 47 patients with various heart diseases. The correlation coefficient between two measurements was 0.766 with a relation of Y=1.04 x-8.48. Also, the high reproducibility was obtained for the same patient. Also, the high reproducibility was obtained for the same patient. From this study, we conclude that this device is useful for continuous monitoring in the intensive care unit, as it is portable, compact, and inexpensive.

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서울시내 수산시장에 유통중인 젓갈류의 세균 분포 (Bacterial Distribution of Salt-Fermented Fishery Products in Seoul Garak Wholesale Market)

  • 함희진;진영희
    • 한국식품위생안전성학회지
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    • 제17권4호
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    • pp.173-177
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    • 2002
  • 2002년 3-5월 서울시 가락농수산물시장에서 구입한 젓갈류 72건(새우젓류 21건, 명태젓류 9건 및 조개젓류 9건, 오징어젓, 밴댕이젓 및 멸치젓 각각 7건, 황세기젓 5건, 꼴뚜기젓 3건 그리고 굴젓 및 갈치젓 각각 2건)을 대상으로 세균분포를 시험한 결과, 평균 염도는 17.2%, 1 ml당 일반세균수는 4,900, 대장균군수 44, 비브리오속균수 160, 포도상구균속균수 3,000 CFU/m1로 각각 집계되었고, 젓갈별 세균 분리 동정결과, 오징어젓에서 13주, 꼴뚜기젓과 추젓에서 각각 11주, 명란젓과 조개젓에서 각각 9주, 멸치젓에서 7주 등 총 93주의 세균이 분리되었다. 세균의 생화학적 분리 동정 결과 대장균군 35.5%(33/93), 비브리오속균 8.6%(8/93) 그리고 포도상구균속균 12.9%(12/93)로 분리되었고, 대장균군의 경우 E. cloacae가 15주로 가장 많았으며, 비브리오속균의 경우 V. alginolyticus가 5주, V. fluvialis가 3주, 포도상구균속균의 경우 S. lentus가 5주로 가장 많았다.

Using Artificial Neural Networks for Forecasting Algae Counts in a Surface Water System

  • Coppola, Emery A. Jr.;Jacinto, Adorable B.;Atherholt, Tom;Poulton, Mary;Pasquarello, Linda;Szidarvoszky, Ferenc;Lohbauer, Scott
    • 생태와환경
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    • 제46권1호
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    • pp.1-9
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    • 2013
  • Algal blooms in potable water supplies are becoming an increasingly prevalent and serious water quality problem around the world. In addition to precipitating taste and odor problems, blooms damage the environment, and some classes like cyanobacteria (blue-green algae) release toxins that can threaten human health, even causing death. There is a recognized need in the water industry for models that can accurately forecast in real-time algal bloom events for planning and mitigation purposes. In this study, using data for an interconnected system of rivers and reservoirs operated by a New Jersey water utility, various ANN models, including both discrete prediction and classification models, were developed and tested for forecasting counts of three different algal classes for one-week and two-weeks ahead periods. Predictor model inputs included physical, meteorological, chemical, and biological variables, and two different temporal schemes for processing inputs relative to the prediction event were used. Despite relatively limited historical data, the discrete prediction ANN models generally performed well during validation, achieving relatively high correlation coefficients, and often predicting the formation and dissipation of high algae count periods. The ANN classification models also performed well, with average classification percentages averaging 94 percent accuracy. Despite relatively limited data events, this study demonstrates that with adequate data collection, both in terms of the number of historical events and availability of important predictor variables, ANNs can provide accurate real-time forecasts of algal population counts, as well as foster increased understanding of important cause and effect relationships, which can be used to both improve monitoring programs and forecasting efforts.

Seasonal and Regional Effects on Milk Composition of Dairy Cows in South Korea

  • Nam, Ki-Taeg;Kim, Ki-Hyun;Nam, In-Sik;Abanto, Oliver D.;Hwang, Seong-Gu
    • Journal of Animal Science and Technology
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    • 제51권6호
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    • pp.537-542
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    • 2009
  • For a period of over 6 years, more than 160,000 milk samples were collected and analyzed to determine the influence of different seasonal temperatures and geographic regional location on milk composition in South Korea. Fat, protein, lactose, non fat milk solids (NFMS) and total solids (TS) contents were significantly higher among dairy cows milked in winter season than other seasons (p<0.05). In contrast, freezing point (FP), milk urea nitrogen (MUN) and somatic cell count (SCC) were significantly higher in summer season than other seasons (p<0.05). The average SCC in the autumn season was $358{\times}10^3$/ml, which was lower than any other seasons (p<0.05). These results may be due to the changes in temperature during different seasons. Meanwhile, milk produced by dairy cows in central region had higher fat, protein, lactose, NFMS, TS and MUN and had lower SCC compared to other regions (p<0.05). Fat, TS, FP, MUN and citric acid in northeast region were lower than other regions (p<0.05). The SCC was significantly higher in southeast region than those of other regions (p<0.05). As a result, it might be possible that the differences in feeding management in each different region may affect the milk composition. In conclusion, present results indicated that milk composition is clearly influenced by both season and regional location. Therefore, based on these results, development of different feeding systems, according to season and region is needed to produce high quality and satiable milk production.