• 한국토양비료학회지
• /
• 제37권3호
• /
• pp.149-155
• /
• 2004

벼 양파 작부체계를 갖는 평택통의 미사질 식양토에서 화학비료의 시용량 절감을 목적으로 돈분뇨액비의 활용 가능성을 검토하였다. 벼 재배지에 사용된 액비는 $N\;3.8g\;kg^{-1}$, $P_2O_5\;1.8g\;kg^{-1}$, $K_2O\;2.7g\;kg^{-1}$을 함유하였고 양파 재배지에 사용된 액비는 $N\;4.9g\;kg^{-1}$, $P_2O_5\;1.4g\;kg^{-1}$, $K_2O\;2.1g\;kg^{-1}$을 함유하였다. 토양 pH는 모든 처리구에서 벼 수확 후 0.2-0.3 정도 상승하였다. 벼 생육과 수량은 화학비료구와 돈분뇨액비 시용구 간에 유의적인 차이가 없었다. 돈분뇨액비를 전량 기비로 시용하였을 때 양파의 중 후기 생육이 불량하였으나 액비기비 + 화학비료추비구와 화학비료구에서의 생육 차이는 없었다. 액비기비 + 화학비료추비구에서 수확기 양파의 양분흡수량는 화학비료표준시비구와 유의적인 차이가 없었다. 양파 수량은 앞그루 벼의 재배 시 화학비료를 시용한 조건에서 액비기비 및 화학비료추비 처리한 구에서 $58.5Mg\;ha^{-1}$로 가장 많았으나 액비전량기비구와 무비구를 제외한 다른 처리구와의 유의적인 차이는 없었다. 이상의 결과를 볼 때 벼 양파 작부체계에서 돈분뇨액비를 활용할 경우 벼 재배 시에는 화학비료를 전량 절감할 수 있었으나 양파는 수량성을 유지하기 위해 돈분뇨액비를 기비기준량으로 시용하고 월동 후 화학비료의 추비가 필요하였다.

• 농약과학회지
• /
• 제4권3호
• /
• pp.68-74
• /
• 2000

Penicillium oxalicum (PENOX)은 잔디밭 토끼풀(Trifolium repens L.)의 방제를 위한 생물제제(biocontrol agent, BCA)로서의 잠재성을 보여왔다. 미생물제초균류에 의한 제초활성은 다양한 환경조건하에서 이들의 발아율, 생장력 및 기질이용성 등에 크게 영향을 받는다. 따라서 본 연구는 PENOX의 다양한 수분확성($0.94{\sim}0.995\;a_w$) 및 온도조건($18{\sim}30^{\circ}C$)하에서의 균사생장, 포자발아율 및 포자생성량을 glycerol로 조정된 MEA(malt extract agar) 배지를 사용하여 in vitro상에서 조사하였다. PENOX의 균사생장은 높은 수분활성조건($0.995\;a_w$)에서 가장 좋았으며 $30^{\circ}C$에서 가장 양호하였다. 또한 $0.995\;a_w$에서는 저온($20^{\circ}C$)에서도 균사생장이 대체로 양호하여 수분활성이 약간 낮은 조건($0.97\;a_w$)에서의 최대 균사생장율과 비슷한 균사생장율을 나타냈다. $0.97\;a_w$에서의 균사생장율은 $25^{\circ}C$와 $30^{\circ}C$조건간에 대체로 비슷한 결과를 나타냈으며 저온($20^{\circ}C$)에서는 매우 감소하였다. PENOX의 포자발아율도 $0.995\;a_w/30^{\circ}C$에서 가장 높았으며 수분활성이 감소할수록 낮아지는 경향을 나타냈다. 저온($18^{\circ}C$)에서의 발아전 지체시간(lag phase time)은 전 $a_w$ 조건하에서 6시간 이상 그리고 낮은 수분활성조건($0.94\;a_w$)하에서는 18과 $25^{\circ}C$에서 각 18, 12시간 이상으로 나타났다. 그러나 포자생성은 $0.995\;a_w$의 높은 수분조건에 비해 약간 낮은 $0.97\;a_w$ 조건에서, 그리고 고온($30^{\circ}C$)에 비해 저온조건($20^{\circ}C$) 하에서 훨씬 양호하였다. 한편, GN MicroPlate를 이용해 PENOX의 기질로서의 95개 탄소원에 대한 이용성을 조사한 결과 $0.995\;a_w/25^{\circ}C$에서의 생태적 생식역값(niche size)은 86으로 가장 높았으며 수분스트레스조건($0.955\;a_w$)에서는 그 이용성이 낮아져 그 값은 65를 나타냈다. 그리고 고온($30^{\circ}C$)에서는 $0.995\;a_w$ 및 $0.955\;a_w$에서는 각각 84와 50을 나타내 $0.995\;a_w$에서 보다 $0.955\;a_w$ 조건에서 탄소원 이용시 온도스트레스에 더 민감하게 반응하는 것으로 나타났다. 이러한 균류생태생리학적인 정보는 효과적인 BCA의 개발을 위한 기초자료로서 유용하게 활용될 수 있을 것이다.

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제8권6호
• /
• pp.338-348
• /
• 2003

In the past decade, considerable progress has been made in developing the appropriate biotechnology for microalgal mass cultivation aimed at establishing a new agro-industry. This review points out the main biological constraints affecting algal biotechnology outdoors and the requirements for making this biotechnology economically viable. One of them is the availability of a wide variety of algal species and improved strains that favorably respond to varying environmental conditions existing outdoors. It is thus just a matter of time and effort before a new methodology like genetic engineering can and will be applied in this field as well. The study of stress physiology and adaptation of microalgae has also an important application in further development of the biotechnology for mass culturing of microalgae. In outdoor cultures, cells are exposed to severe changes in light and temperature much faster than the time scale re-quired for the cells to acclimate. A better understanding of those parameters and the ability to rapidly monitor those conditions will provide the growers with a better knowledge on how to optimize growth and productivity. Induction of accumulation of high value products is associated with stress conditions. Understanding the physiological response may help in providing a better production system for the desired product and, at a later stage, give an insight of the potential for genetic modification of desired strains. The potential use of microalgae as part of a biological system for bioremediation/detoxification and wastewater treatment is also associated with growing the cells under stress conditions. Important developments in monitoring and feedback control of the culture behavior through application of on-line chlorophyll fluorescence technique are in progress. Understanding the process associated with those unique environmental conditions may help in choosing the right culture conditions as well as selecting strains in order to improve the efficiency of the biological process.

• Asian-Australasian Journal of Animal Sciences
• /
• 제16권4호
• /
• pp.625-634
• /
• 2003

Oil palm fronds (OPF) are one of the main by-products of the oil palm industry in Malaysia. It contains about 38.5 % crude fibre with ME values of about 5.65 MJ/kg dry matter. OPF has great potential to be utilized as a roughage source or as a component in a complete feed for ruminant animals. This paper briefly reviews the availability of OPF in Malaysia and its importance in the local beef and dairy industry. About 26 million metric tonnes of OPF are produced on dry matter basis annually during pruning and replanting operations in the plantations. The nutritive value of OPF and studies to improve its feeding value is highlighted. The optimum level of inclusion for ruminant feeding is 30 % and improvement to intake and digestibility can be further enhanced with addition of other oil-palm by-products. Performances of beef and dairy cattle fed fresh OPF or as silage, pellets and cubes are shown. Good quality OPF silage can be produced without using any additive and the significant improvement on the rate of growth and milk yield were shown. With good formulations, OPF based diets can allow live weight gains of between 600-850 g/day and for local crossbred dairy animals, milk yield of about 11.1 to 20.3 liter/day can be obtained. Pellet based on ground OPF seemed to be less well utilized for ruminant feeding due to its smaller particle size. OPF based cubes which have longer particle size is more suitable for beef and dairy cattle. Long-term feeding of OPF based feeds have been shown to produce good quality carcasses, and the meat is safe for consumption.

• Asian-Australasian Journal of Animal Sciences
• /
• 제20권1호
• /
• pp.41-44
• /
• 2007

The callipyge mutation in sheep is a polar overdominant mutation that results in post-natal muscle hypertrophy in the loin and hindquarters of paternal heterozygotes (+/CLPG). Sheep that are homozygous for the callipyge allele (CLPG/CLPG) do not express the muscle hypertrophy phenotype, but serve as carriers for the mutation. Callipyge sheep are characterized by improved feed efficiencies and leaner carcasses. Leptin is a protein hormone secreted from adipose tissue and has been found to affect appetite and serve as an indicator of body fat mass. To date, very little knowledge is available as to the effect of the callipyge mutation on circulating leptin levels. Due to the interaction of leptin with feed intake and energy availability, and the fact that the majority of fetal growth occurs in late gestation, it is important to understand if the callipyge mutation interacts with leptin production in late gestational ewes. Therefore, our objective was to characterize serum concentrations of leptin in late gestational callipyge ewes vs. non-callipyge ewes. We evaluated genetically verified callipyge (n=6), homozygous (n=8) and normal (n=8) ewes weekly during the last eight wks of gestation through one wk post-partum. Weights were taken and body condition scores were assigned by trained personnel weekly. Blood was collected via jugular venipuncture on each sampling date and subjected to an ovine-specific leptin RIA. Genotype influences on peripheral concentrations of leptin were found to be highly significant (p=0.0005). Total leptin means for +/CLPG were 5.41${\pm}$0.40 ng/ml, CLPG/CLPG 8.11${\pm}$0.70 ng/ml, and +/+ 9.13${\pm}$0.93 ng/ml. Sampling date was also significant (p=0.0098) with all ewes showing a decrease in leptin levels throughout gestation and parturition. Using repeated measures, we were able to detect lower levels of plasma leptin in callipyge ewes, which may be indicative of their lower overall body fat content. These results indicate that the callipyge phenotype decreases the levels of adipose tissue and leptin production in gestating ewes.

• 한국중재학회지:중재연구
• /
• 제20권2호
• /
• pp.27-47
• /
• 2010

It is well recognized that the availability of prompt, effective and economical means of dispute resolution is an important element in the orderly growth and encouragement of international trade and investment. Increasingly, arbitration, instead of litigation in national courts, has become the preferred means of resolving private international commercial disputes. Under the situation, it will be important thing for arbitral institutions to reach an agreement to promote the dispute settlement of the commercial disputes, for which efforts have been made between the Korean Commercial Arbitral Board(KCAB) and principal arbitration institutions of the foreign countries. Since 1973, the KCAB has entered into many arbitration agreements with well-known foreign institutions of arbitration. If the place of arbitration is not so designated by the parties, it, as a general rule, shall be the country of the respondent(s) under the Korea-Japanese Arbitration Agreement. On the other hand, the U.S.-Korean Commercial Arbitration Agreement maintains 'Joint Arbitration Committee which finally decide the place of arbitration. In 1996, the Korea-Austria Agreement of Cooperation was concluded for the prompt and equitable settlement on an amicable basis of commercial disputes. Under this Agreement, arbitral institutions between Korea and Austria agreed to act as an appointing authority in accordance with the UNCITRAL Arbitration Rules. It is also very important for Korea and China including North Korea to cooperate each other for the settlement of the commercial disputes within the Pan Yellow Sea Economic Bloc(PYSEB). The PYSEB is quickly becoming a distinctive and crucial region in the world sharing geographical proximity, many common historical experiences, and similar cultural norms and values although they have disparities in stages of development, trade and economic policies, and financial and legal frameworks. Finally, it should be considered to establish a central common system for settlement promotion of the commercial disputes within the PYSEB through the arbitration agreement. Such a dispute resolution system was already introduced and established within the area of the NAFTA, and it is called the Commercial Arbitration and Mediation Center for the Americas(CAMCA).

• 한국토양비료학회지
• /
• 제46권5호
• /
• pp.365-372
• /
• 2013

Crop development and nutrient availability are strongly influenced by soil salinity levels. This study was conducted to investigate the effect of rice straw and nitrogen (N) fertilizer for silage barley under various soil salinity levels at Saemangeum reclaimed tidal land. Three levels of rice straw (0, 2.5, 5.0 ton rice straw $ha^{-1}$) and N (0, 150, 225 kg N $ha^{-1}$) were applied at 0.04, 0.23, 0.35% soil salinity levels. Biomass yield of silage barley was influenced by the interactions between rice straw application and N fertilization. Although there was no single effect of rice straw application on biomass yield, it was significantly increased with N application and a rice straw application of 5.0 ton $ha^{-1}$. Sodium content in silage barley was significantly lower at 0.04% salinity level, and but it was statistically increased with increasing soil salinity levels. Forage qualities such as total digestible nutrients and relative feed value of silage barley were significantly higher with N application at 0.04% salinity level, but there was no effect of rice straw application. Soil organic matter content was increased with N and rice straw application regardless of soil salinity level. The results of this study showed that the effect of rice straw and N fertilization on silage barley was influenced by soil salinity levels, which indicates that the management practice of silage barley at Saemangeum reclaimed tidal land should consider soil salinity levels.

• Asian-Australasian Journal of Animal Sciences
• /
• 제30권8호
• /
• pp.1183-1189
• /
• 2017

Objective: Owing to the public availability of complete genome sequences, including avian species, massive bioinformatics analyses may be conducted for computational gene prediction and the identification of gene regulatory networks through various informatics tools. However, to evaluate the biofunctional activity of a predicted target gene, in vivo and in vitro functional genomic analyses should be a prerequisite. Methods: Due to a lack of quail genomic sequence information, we first identified the partial genomic structure and sequences of the quail SH3 domain containing ring finger 2 (SH3RF2) gene. Subsequently, SH3RF2 was knocked out using clustered regularly interspaced short palindromic repeat/Cas9 technology and single cell-derived SH3RF2 mutant sublines were established to study the biofunctional activity of SH3RF2 in quail myoblast (QM7) cells during muscle differentiation. Results: Through a T7 endonuclease I assay and genotyping analysis, we established an SH3RF2 knockout (KO) QM7#4 subline with 61 and 155 nucleotide deletion mutations in SH3RF2. After the induction of myotube differentiation, the expression profiles were analyzed and compared between regular QM7 and SH3RF2 KO QM7#4 cells by global RNA sequencing and bioinformatics analysis. Conclusion: We did not detect any statistically significant role of SH3RF2 during myotube differentiation in QM7 myoblast cells. However, additional experiments are necessary to examine the biofunctional activity of SH3RF2 in cell proliferation and muscle growth.

• 대한한방부인과학회지
• /
• 제30권2호
• /
• pp.37-48
• /
• 2017

Objectives: Artemisiae Annuae Herba is the dried aerial part of Artemisia annua L. (AAL). In Oriental medicine, Artemisiae Annuae Herba (AAH) is traditionally used to treat fever. AAH clears summerheat or damp-Heat, clears deficiency fevers, cools the blood and stops bleeding, stops malarial disorders and relieves heat, clears liver heat and brightens the eyes. Recently, there were many studies about effects of AAH on anti-oxidative, anti-inflammatory, anti-cancer, hair growth and plasma lipid composition. So, we expected AAH has an availability that can effect on skin whitening and elasticity. Methods: The present study was designed to investigate the effects of AAH on skin whitening and elasticity in SK-MEL-2 cells. In this experiment, the effects of AAH on proliferation rates, melanin synthesis, tyrosinase activities and production levels of tyrosinase, MMP-1 and MMP-9 in vitro were examined. Results: AAH did not affect viability of SK-MEL-2 cells and inhibited melanin synthesis induced by ${\alpha}$-Melanocyte-stimulating hormone (${\alpha}$-MSH) significantly. In addition, AAH also inhibited tyrosinase activity and lowered tyrosinase level in SK-MEL-2 cells. Finally, AAH inhibited productions of Matrix metalloproteinase-1 (MMP-1) and Matrix metalloproteinase-9 (MMP-9). Conclusions: These data suggest that AAH can be used to treat patients with skin diseases such as freckled face and also used as skin whitening agent.

• Asian-Australasian Journal of Animal Sciences
• /
• 제14권7호
• /
• pp.979-985
• /
• 2001

Two experiments on commercial broiler chickens (3-37 d) and WL layers (252-364 d) were conducted to study the relative bioavailability of phosphorus (P) from different P supplements in comparison to tricalcium phosphate (TCP), at constant dietary calcium (Ca):P ratio. The P sources tested were dicalcium phosphate (DCP), steam sterilized bone meal (SSBM), commercial mineral mixture (CMM), phosphoric acid (PA) and a combination of CMM + PA. Fluorine (F) content in CMM and SSBM was 13.12 and 0.14 g/kg, respectively. In commercial broiler diets, DCP, SSBM or PA could be used as supplemental P sources without affecting (p<0.05) weight gain, feed intake, tibia ash and, P and Ca contents in tibia ash when compared to TCP. Severity of leg abnormality and deposition of F in bone were higher (p<0.05) in group fed CMM. P retention and serum inorganic P content was significantly reduced (p<0.05) in CMM fed birds compared to those fed TCP, SSBM, PA or CMM+PA. Among other P sources (TCP, SSBM, PA and CMM+PA) the serum inorganic P levels did not vary significantly (p<0.05). The P retention also significantly reduced in CMM fed groups compared to those fed DCP or PA. The P retention significantly increased from 0.183 to 0.216 units by supplementation of PA to CMM diet. In layers, egg production was not affected by replacing TCP with DCP, SSBM, PA or CMM+PA, but significantly (p<0.05) reduced with CMM. Feed (kg)/kg egg mass, egg weight, shell quality (shell weight and shell thickness) and serum Ca levels were not influenced by dietary variation in P source. The poor performance of both broilers and layers fed on CMM based diets could be attributed to the presence of hlgher levels of F (647.8 and 630.1 mg/kg, respectively) and low P utilization. Based on growth, bone mineralization and P retention it is concluded that DCP, SSBM or PA can be used as alternatives to TCP in broiler diets. In WL layer diets, in addition to above P sources, CMM can also be used as supplemental P source by replacing one half of P from CMM with PA without affecting egg production and shell quality.