The Journal of Korean Obstetrics and Gynecology (대한한방부인과학회지)

The Society of Korean Medicine Obstetrics and Gynecology (대한한방부인과학회)
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Inhibitory Effect of Artemisiae Annuae Herba Extracts on Melanin Synthesis, Tyrosinase Activities and Production Levels of Tyrosinase, MMP-1 and MMP-9 in SK-MEL-2 Cells

SK-MEL-2 세포에서 청호(靑蒿) 추출물의 Melanin 생성, Tyrosinase 활성과 생성, MMP-1 및 MMP-9 생성 억제 효과

  • Lee, Eun-Kyu (Dep. of Korean Gynecology and Obstetrics, College of Korean Medicine, Dong-Shin University) ;
  • Kim, Soo-Hyeon (Dep. of Korean Gynecology and Obstetrics, College of Korean Medicine, Dong-Shin University) ;
  • Park, Kyung-Mi (Dep. of Korean Gynecology and Obstetrics, College of Korean Medicine, Dong-Shin University) ;
  • Yang, Seung-Jeong (Dep. of Korean Gynecology and Obstetrics, College of Korean Medicine, Dong-Shin University) ;
  • Cho, Seong-Hee (Dep. of Korean Gynecology and Obstetrics, College of Korean Medicine, Dong-Shin University)
  • 이은규 (동신대학교 한의과대학 한방부인과교실) ;
  • 김수현 (동신대학교 한의과대학 한방부인과교실) ;
  • 박경미 (동신대학교 한의과대학 한방부인과교실) ;
  • 양승정 (동신대학교 한의과대학 한방부인과교실) ;
  • 조성희 (동신대학교 한의과대학 한방부인과교실)
  • Received : 2017.04.24
  • Accepted : 2017.05.22
  • Published : 2017.05.26
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Abstract

Objectives: Artemisiae Annuae Herba is the dried aerial part of Artemisia annua L. (AAL). In Oriental medicine, Artemisiae Annuae Herba (AAH) is traditionally used to treat fever. AAH clears summerheat or damp-Heat, clears deficiency fevers, cools the blood and stops bleeding, stops malarial disorders and relieves heat, clears liver heat and brightens the eyes. Recently, there were many studies about effects of AAH on anti-oxidative, anti-inflammatory, anti-cancer, hair growth and plasma lipid composition. So, we expected AAH has an availability that can effect on skin whitening and elasticity. Methods: The present study was designed to investigate the effects of AAH on skin whitening and elasticity in SK-MEL-2 cells. In this experiment, the effects of AAH on proliferation rates, melanin synthesis, tyrosinase activities and production levels of tyrosinase, MMP-1 and MMP-9 in vitro were examined. Results: AAH did not affect viability of SK-MEL-2 cells and inhibited melanin synthesis induced by ${\alpha}$-Melanocyte-stimulating hormone (${\alpha}$-MSH) significantly. In addition, AAH also inhibited tyrosinase activity and lowered tyrosinase level in SK-MEL-2 cells. Finally, AAH inhibited productions of Matrix metalloproteinase-1 (MMP-1) and Matrix metalloproteinase-9 (MMP-9). Conclusions: These data suggest that AAH can be used to treat patients with skin diseases such as freckled face and also used as skin whitening agent.

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References

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