• Current Applied Physics
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• v.18 no.11
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• pp.1185-1189
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• 2018

Thickness-dependent magnetic domain structure of ultrathin Co wedge films (0.3 nm-1.0 nm) sandwiched by Pt layers was investigated by scanning transmission x-ray microscopy (STXM) employing X-ray magnetic circular dichroism (XMCD), utilizing elliptically polarized soft x-rays and electromagnetic fields, with a spatial resolution of 50 nm. The magnetic domain images measured at the Co $L_3$ edge showed the evolution of the magnetic domain structures from maze-like form to the bubble-like form as the perpendicular magnetic field was applied. The asymmetric domain expansion of a 500 nm-scale bubble domain was also measured when the in-plane and perpendicular external magnetic field were applied simultaneously.

• Biodesign
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• v.7 no.1
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• pp.24-27
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• 2019

Pnc1 converts nicotinamide to nicotinic acid to generate NAD⁺ through the Preiss-Handler pathway that is one of the NAD⁺-salvage pathway. By reducing levels of nicotinamide, an inhibitor of the NAD⁺-dependent histone deacetylase Sir2, yeast Pnc1 contributes gene silencing. In this study, to understand the structural features and molecular mechanism of nicotinamidase Pnc1, we overexpressed, purified, and crystallized the N-terminally His₆-tagged Pnc1 protein from Kluyveromyces lactis and obtained X-ray diffraction data at a resolution of 2.2 Å. The crystals of the K. lactis Pnc1 (KlPnc1) belonged to space group P2₁2₁2₁ with unit cell parameters a=38.5, b=77.3, c=83.3, and α=β=γ= 90°. There is one molecule in the asymmetric unit.

• 한국해양학회지
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• v.27 no.1
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• pp.66-77
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• 1992

Tidal sand ridges and sand waves are well developed in the Yellow Sea off Tae-An Peninsula, Korea. Their transport directions were inferred based on high resolution seismic profiles, surficial sediment characteristics and tidal regime. Tidal sand ridges are asymmetric, with long axes parallel to or slightly oblique to the dominant NE-SW tidal current direction. They show steep south and/or southeast flanks, which are in concordance with the apparent direction of internal cross-bedding in the south. Holocene sediments occur in accordance with distributional patterns of tidal sand ridges. These features indicate that Holocene active tidal sand ridges move toward the open sea in southeast, south and southwest direction. Sand waves which are distributed in flat sea floor with depth of about 40-60m show also asymmetric forms with a steep east-to-northeast face. Surficial sediments in the sand wave field are characterized by well sorted fine sands compared with poorly sorted adjacent areas. The sand waves appear to undergo easterly or northeasterly landward movement.

• Journal of The Korean Astronomical Society
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• v.55 no.5
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• pp.149-172
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• 2022

We present a new method which constructs an Hɪ super-profile of a galaxy which is based on profile decomposition analysis. The decomposed velocity profiles of an Hɪ data cube with an optimal number of Gaussian components are co-added after being aligned in velocity with respect to their centroid velocities. This is compared to the previous approach where no prior profile decomposition is made for the velocity profiles being stacked. The S/N improved super-profile is useful for deriving the galaxy's global Hɪ properties like velocity dispersion and mass from observations which do not provide sufficient surface brightness sensitivity for the galaxy. As a practical test, we apply our new method to 64 high-resolution Hɪ data cubes of nearby galaxies in the local Universe which are taken from THINGS and LITTLE THINGS. In addition, we also construct two additional Hɪ super-profiles of the sample galaxies using symmetric and all velocity profiles of the cubes whose centroid velocities are determined from Hermite h3 polynomial fitting, respectively. We find that the Hɪ super-profiles constructed using the new method have narrower cores and broader wings in shape than the other two super-profiles. This is mainly due to the effect of either asymmetric velocity profiles' central velocity bias or the removal of asymmetric velocity profiles in the previous methods on the resulting Hɪ super-profiles. We discuss how the shapes (𝜎_n/𝜎_b, A_n/A_b, and A_n/A_tot) of the new Hɪ super-profiles which are measured from a double Gaussian fit are correlated with star formation rates of the sample galaxies and are compared with those of the other two super-profiles.

• Journal of the Korean earth science society
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• v.36 no.6
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• pp.553-566
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• 2015

The effects of vertical resolutions and planetary boundary layer (PBL) physics schemes in a numerical simulation with a very high resolution over the metropolitan area were investigated. The numerical experiments using the Weather Research and Forecast model were conducted from 0000 UTC 25 October to 0000 UTC 26 October 2013. We verified the numerical results against with six hourly observation data from the radiosonde at Seolleung, which was located in southern part of Seoul, and forty three auto weather systems in Seoul. In the experiments of vertical resolutions in low level atmosphere with 44, 50, and 60 layers, which are set to be subdivided particularly under 2 km height. The experiment in 60 layers, which has the highest vertical resolution in this study, showed relatively a clear diurnal variation of PBL heights. Especially, the difference of PBL heights and 10-meter wind fields were mainly seen in the area of high altitude lands for the experiments of vertical resolution. In the sensitivity experiment of PBL schemes such as asymmetric convective model-version 2 (ACM2), Yonsei University (YSU), and Mellow-Yamada-Janjic (MYJ) to the temperature, all three PBL schemes revealed lower temperature than observed profile from the radiosonde in the entire period. The experiments with YSU PBL and ACM2 PBL schemes show relatively less biased in comparison with the experiment of the MYJ PBL scheme.

• Progress in Superconductivity
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• v.14 no.2
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• pp.99-101
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• 2012

Fluorescent yield X-ray absorption fine structure (XAFS) spectroscopy is useful for analyzing local structure of specific elements in matrices. We developed an XAFS apparatus with a 100-pixel superconducting tunnel junction (STJ) detector array with a high sensitivity and a high resolution for light-element dopants in wide-gap semiconductors. An STJ detector has a pixel size of $100{\mu}m$ square, and an asymmetric layer structure of Nb(300 nm)-Al(70 nm)/AlOx/Al(70 nm)-Nb(50 nm). The 100-pixel STJ array has an effective area of $1mm^2$. The XAFS apparatus with the STJ array detector was installed in BL-11A of High Energy Accelerator Research Organization, Photon Factory (KEK PF). Fluorescent X-ray spectrum for boron nitride showed that the average energy resolution of the 100-pixels is 12 eV in full width half maximum for the N-K line, and The C-K and N-K lines are separated without peak tail overlap. We analyzed the N dopant atoms implanted into 4H-SiC substrates at a dose of 300 ppm in a 200 nm-thick surface layer. From a comparison between measured X-ray Absorption Near Edge Structure (XANES) spectra and ab initio FEFF calculations, it has been revealed that the N atoms substitute for the C site of the SiC lattice.

• Journal of The Korean Astronomical Society
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• v.38 no.2
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• pp.219-222
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• 2005

We present the high-resolution (2"-4") images of the molecular envelopes surrounding the evolved stars, V Hya, VY CMa, and ${\pi}^1$ Gru observed with the Submillimeter Array. The CO J=2-1 and 3-2 images of the carbon star V Hya show that the circumstellar structure of this star consists of three kinematic components; there is a flattened disk-like envelope that is expanding with a velocity of ${\~}16 km\;s^{-1}$, the second component is the medium-velocity wind having a deprojected velocity of 40-120 km $s^{-l}$ moving along the disk plane, and the third one is the bipolar molecular jet having an extreme velocity of 70-185 km $s^{-l}$. The axis of this high velocity jet is perpendicular to the plane of the disk-like envelope. We found that the circumstellar structure of the S-star ${\pi}^1$ Gru traced by the CO J =2-1 resembles that of V Hya quite closely; the star is surrounded by the expanding disk-like envelope and is driving the medium-velocity wind along the disk plane. We also obtained the excellent images of VY CMa with the CO and $^{13}CO$ J=2-1 and $SO\;6_5-5_4$ lines. The maps of three molecular lines show that the envelope has a significant velocity gradient in the east-west direction, suggesting that the envelope surrounding VY CMa is also flattened and expanding along its radial direction. The high-resolution images obtained with the SMA show that some AGB stars are associated with the asymmetric mass loss including the equatorial wind and bipolar jet.

• Journal of Microbiology and Biotechnology
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• v.18 no.2
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• pp.283-286
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• 2008

The methylenetetrahydrofolate dehydrogenase/cyclohydrolase (MTHFDC) from the thermoacidophilic archaeon Thermoplasma acidophilum is a 30.6kDa molecular-mass enzyme that sequentially catalyzes the conversion of formyltetrahydrofollate to methylenetetrahydrofolate, with a preference for NADP as a cofactor, rather than NAD. In order to elucidate the functional and structural features of MTHFDC from archaeons at a molecular level, it was overexpressed in Escherichia coli and crystallized in the presence of its cofactor, NADP, at 295K using polyethylene glycol (PEG) 4000 as a precipitant. The crystal is a member of the monoclinic space group $P2_1$, with the following unit cell parameters: $a=66.333{\AA},\;b=52.868{\AA},\;c=86.099{\AA},\;and\;{\beta}=97.570^{\circ}$, and diffracts to a resolution of at least $2.40{\AA}$ at the synchrotron. Assuming a dimer in the crystallographic asymmetric unit, the calculated Matthews parameter $(V_M)\;was\;2.44{\AA}^3/Da$ and the solvent content was 49.7%.

• Journal of Microbiology and Biotechnology
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• v.18 no.2
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• pp.295-298
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• 2008

Creatine kinase (CK; E.C. 2.7.3.2) is an important enzyme that catalyzes the reversible transfer of a phosphoryl group from ATP to creatine in energy homeostasis. The brain-type cytosolic isoform of creatine kinase (BB-CK), which is found mainly in the brain and retina, is a key enzyme in brain energy metabolism, because high-energy phosphates are transfered through the creatine kinase/phosphocreatine shuttle system. The recombinant human BB-CK protein was overexpressed as a soluble form in Escherichia coli and crystallized at $22^{\circ}C$ using PEG 4000 as a precipitant. Native X-ray diffraction data were collected to $2.2{\AA}$ resolution using synchrotron radiation. The crystals belonged to the tetragonal space group $P4_32_12$, with cell parameters of a=b=97.963, $c=164.312{\AA},\;and\;{\alpha}={\beta}={\gamma}=90^{\circ}$. The asymmetric unit contained two molecules of CK, giving a crystal volume per protein mass $(V_m)$ of $1.80{\AA}^3\;Da^{-1}$ and a solvent content of 31.6%.

• Journal of Microbiology and Biotechnology
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• v.28 no.4
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• pp.597-605
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• 2018

Acyl-CoA oxidases (ACOXs) play important roles in lipid metabolism, including peroxisomal fatty acid ${\beta}$-oxidation by the conversion of acyl-CoAs to 2-trans-enoyl-CoAs. The yeast Yarrowia lipolytica can utilize fatty acids as a carbon source and thus has extensive biotechnological applications. The crystal structure of ACOX3 from Y. lipolytica (YlACOX3) was determined at a resolution of $2.5{\AA}$. It contained two molecules per asymmetric unit, and the monomeric structure was folded into four domains; $N{\alpha}$, $N{\beta}$, $C{\alpha}1$, and $C{\alpha}2$ domains. The cofactor flavin adenine dinucleotide was bound in the dimer interface. The substrate-binding pocket was located near the cofactor, and formed at the interface between the $N{\alpha}$, $N{\beta}$, and $C{\alpha}1$ domains. Comparisons with other ACOX structures provided structural insights into how YlACOX has a substrate preference for short-chain acyl-CoA. In addition, the structure of YlACOX3 was compared with those of medium- and long-chain ACOXs, and the structural basis for their differences in substrate specificity was discussed.