• 한국식품과학회지
• /
• 제28권5호
• /
• pp.801-805
• /
• 1996

알루미늄 화합물이 흰쥐의 효소활성에 미치는 영향을 알아보고자 흰쥐(Sprague-Dawley계 male) 75마리를 5개 그룹 즉, 대조군, $250\;ppm\;AlCl_3\;500\;ppm\;AlCl_3,\;250\;ppm\;Al_2(SO_4)_3,\;500\;ppm\;Al_2(SO_4)_3$로 나누어 2주동안 투여하여 asparate amino transaminase, alanine amino transminase, lactate dehydrogenase, 그리고 cholinesterase의 변화를 조사하였다. 체중은 대조군에 비하여 $Al_2(SO_4)_3$ 투여군은 2.82-6.16% 증가한 반면에 $AlCl_3$ 투여군에서는 0.53-3.35%로 감소하였다. 효소활성은 대조군에 비하여 $AlCl_3$ 또는 $Al_2(SO_4)_3$ 투여후에 AST는 0.68-9.97%, 21.04-24.79%, LDH는 29.43-57.56%, 74.60-29.33% 증가하였으나, ALT는 12.69-25.42%, 24.32-39.62%로 각각 감소하였다. ChE는 대조군에 비하여 $Al_{2}(SO_4)_3$ 투여군은 28.0-12.73% 증가하였으나 $Al_2(SO_4)_3$ 투여군은 3.93-14.48% 감소하였다.

• Journal of Microbiology and Biotechnology
• /
• 제15권1호
• /
• pp.136-140
• /
• 2005

The hypoglycemic effect of Collybia confluens exobiopolymer was investigated in streptozotocin (STZ)-induced diabetic rats. In a dose-dependent study, the exobiopolymer, at 150 mg/kg body weight (BW) dose substantially lowered the plasma glucose level by 29.3%, as compared to the control group. It also lowered the plasma total cholesterol and triglyceride levels by 23.3 and 30.7%, respectively, and reduced the liver total cholesterol and triglyceride levels by 23.0 and 33.5%, respectively. The activity of alanine transaminase (ALT) and aspartate transaminase (AST) was reduced 34.6% and 23.6% respectively, by the exobiopolymer administration, compared to the control group. The exobiopolymer was found to contain 83.2% carbohydrate and 16.8% protein. The sugar and amino acid of the exobiopolymer were also analyzed in detail.

• 한국토양비료학회지
• /
• 제7권4호
• /
• pp.209-213
• /
• 1974

수도근(水稻根)의 Glutamic oxalacetic transaminase isozyme을 DEAE-cellulose column chromatography에 의(依)해서 분리(分離)하고 그에 대(對)한 몇가지 성질(性質)을 구명(究明)하였든 바 얻어진 결과(結果)를 요약(要約)하면 다음과 같다. 1. 수도근(水稻根)의 GOT 활성(活性)은 93%가 Supernatant 분획(分劃)에 존재(存在)하고 Mitochondria에는 5%정도(程度)의 활성(活性)을 보이고 비활성도(比活性度)도 같은 경향(傾向)으로 거의 대부분(大部分)이 Supernatant 분획(分劃)에 국재(局在)해 있다. 2. 수도근(水稻根)의 GOT isozyme은 Cationic GOT로 존재(存在)하나 모두 Sepernatant 분획(分劃)에 국재(局在)하는 것 같다. 3. GOT isozyme의 pH에 대(對)한 반응성(反應性)은 모두 7.4에서 최고(最高)의 활성도(活性度)를 나타내었다. 4. GOT isozmye의 열안정성(熱安定性)은 $70^{\circ}C$에서 Anionic GOT가 더 불안정(不安定)하다. 5. 수도근(水稻根)의 GOT isozyme의 L-aspartic acid에 대(對)한 Km치(値)는 Cationic GOT에서 14mM, Anionic GOT는 16mM을 나타내고 있다.

• 한국식생활문화학회지
• /
• 제30권4호
• /
• pp.468-474
• /
• 2015

This study was designed to investigate the effects of Korean Lycii fructus water extract in Al (Aluminum) administered rats. Forty-eight male Sprague-Dawley rats were divided into six groups: Control group, water extract group with 3% Lycii fructus, 1000 and 2000 ppm of Al groups, and 1000 and 2000 ppm of Al with 3% Lycii fructus water extract group. The Al content of rat tissue in the Al administered group was lower than that in rat tissue in the Al with 3% Lycii fructus water extract group. Plasma levels of renin and aldosterone activity was higher in the Al administration group, compared with the 3% Lycii fructus water extract group and Al administered group. Aspartate amino transaminase and alanine amino transaminase activities were elevated in the Al administered group and lower in the 3% Lycii fructus water extract group. Lactate dehydrogenase was lower in the 3% Lycii fructus water extract Al group than in the Al group. Choline acetyltransferase was higher in the 3% Lycii fructus water extract Al group than in the Al group.

• Archives of Pharmacal Research
• /
• 제29권1호
• /
• pp.73-79
• /
• 2006

This investigation was undertaken to study the effects of oral administration (3 weeks) of Collybia confluens mycelial powder (CCMP) produced by a submerged culture on plasma glucose and other biochemical parameters in streptozotocin (STZ)-induced diabetic rats. Antidiabetic and hypolipidemic effects were proportionally increased with the increasing concentration of the CCMP for oral administration. The CCMP, at the dose of 400 mg/kg BW, substantially reduced the plasma glucose level by as much as 33.1 % as compared to the STZ-induced diabetic rats group. It also lowered the plasma total cholesterol, triglyceride, and low density lipoprotein (LDL) cholesterol by 22.9%, 19.9%, and 37.3%, respectively. The levels of total cholesterol and triglyceride in liver were reduced to the extent of by 13.5% and 18.8%, and the activity of alanine transaminase (ALT) and aspartate transaminase (AST) was decreased by 48.8% and 37.2%, respectively, under the influence of CCMP. The general components of CCMP were found to contain 26.18% carbohydrate, 3.67% crude ash, 4.02% crude fat, 22.55% crude protein, and 43.58% dietary fiber. The amino acid composition of the CCMP was also analyzed in detail.

• 한국식품영양학회지
• /
• 제20권2호
• /
• pp.173-178
• /
• 2007

In this study we manufactured mulberry fruit tea(MFT) to be used as an anti-inflammatory agent. Here, MFT was consumed by thirty middle-aged women with symptoms of rheumatoid arthritis for 4 weeks. The anthropometric measurements and serum inflammatory factors were measured before and after consumption of the MFT. For the anthropometric measurements, BMI and body fat decreased after 4 weeks of tea consumption although there were no significant differences. The serum inflammatory factors(uric acid and homocysteine) as well as serum amino transferase activity(aspartate trans-aminase and alanine transaminase) showed a decreasing trend. In addition, serum carbon reactive protein(CRP) decreased significantly decreased after MFT consumption 4 weeks. It's concluded that this MFT is helpful to for rheumatyroid arthritis patients in order to decrease serum inflammatory factors such as CRP.

• Asian-Australasian Journal of Animal Sciences
• /
• 제31권1호
• /
• pp.106-115
• /
• 2018

Objective: The goal of this study was to investigate the effects of dietary standard ileal digestible (SID) valine:lysine ratios on performance, intestinal morphology, amino acids of liver and muscle, plasma indices and mRNA expression of branched-chain amino acid (BCAA) metabolism enzymes. Methods: A total of 144 crossbred pigs (Duroc${\times}$Landrace${\times}$Large White) weaned at $28{\pm}4days$ of age ($8.79{\pm}0.02kg$ body weight) were randomly allotted to 1 of 4 diets formulated to provide SID valine:lysine ratios of 50%, 60%, 70%, or 80%. Each diet was fed to 6 pens of pigs with 6 pigs per pen (3 gilts and 3 barrows) for 28 days. Results: Average daily gain increased quadratically (p<0.05), the villous height of the duodenum, jejunum and ileum increased linearly (p<0.05) as the SID valine:lysine ratio increased. The concentrations of plasma ${\alpha}-keto$ isovaleric and valine increased linearly (p<0.05), plasma aspartate, asparagine and cysteine decreased (p<0.05) as the SID valine:lysine ratio increased. An increase in SID lysine:valine levels increased mRNA expression levels of mitochondrial BCAA transaminase and branched-chain ${\alpha}-keto$ acid dehydrogenase in the longissimus dorsi muscle (p<0.05). Conclusion: Using a quadratic model, a SID valine:lysine ratio of 68% was shown to maximize the growth of weaned pigs which is slightly higher than the level recommended by the National Research Council.

• Journal of Acupuncture Research
• /
• 제31권4호
• /
• pp.33-43
• /
• 2014

This study was performed to investigate the protective effects of acupuncture on the liver in the oxidative stress caused by cadmium accumulation. Sprague-Dawley male($150{\pm}30g$) rats were stabilized for a week and divided into 5 groups which is normal, control, $LR_3$ acupuncture, $BL_{23}$ acupuncture and sham acupuncture group. For three days experimental groups were received oral doses of cadmium 2 mg/kg twice a day. Acupuncture was given bilaterally at each point 10 times for two weeks. The depth of stimulation was 1 mm at right angles and torsion of acupuncture was produced 2 times per second for 1 minutes. The liver was shipped off and taken weight at the last day of two weeks, and hepatic functions was confirmed through alanine transaminase(ALT) and aspartate amino-transferase(AST). We measured reactive oxygen species of serum, liver and kidney, and compared expression levels of superoxide dismutase(SOD), catalase, glutathione peroxidase(Gpx), nuclear factor erythroid derived 2-related factor 2(Nrf-2), heme oxygenase-1(HO-1), nuclear factor-${\kappa}B$ (NF-${\kappa}B$), cyclooxygenase-2(COX-2), inducible nitric oxide synthase(iNOS), Bax and Cytochrome c. $BL_{23}$ acupuncture group significantly increased liver weight and decreased ALT compared to control group. For the oxidative stress, $LR_3$ acupuncture group significantly reduced reactive oxygen species, and $BL_{23}$ acupuncture group significantly reduced reactive oxygen species and inflammation-related protein compared to control group. But $LR_3$ acupuncture group and $BL_{23}$ acupuncture group didn't significantly reduce apoptosis-related protein. Therefore $LR_3$ and $BL_{23}$ acupuncture showed the effects of antioxidant and anti-inflammatory, especially $BL_{23}$ acupuncture was more effective than $LR_3$ acupuncture on the protection of liver in the oxidative stress.