Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
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The Protective Effects of Acupuncture on the Liver in the Oxidative Stress Caused by Cadmium

카드뮴으로 유발된 산화적 스트레스에 대한 침 자극의 간 보호 효과

  • Shin, Hwa Young (Department of Acupuncture, Moxibustion, Meridian & Acupoint, Colleage of Oriental Medicine, Daegu Hanny University) ;
  • Lee, Hyun Jong (Department of Acupuncture, Moxibustion, Meridian & Acupoint, Colleage of Oriental Medicine, Daegu Hanny University) ;
  • Lim, Seong Chul (Department of Acupuncture, Moxibustion, Meridian & Acupoint, Colleage of Oriental Medicine, Daegu Hanny University) ;
  • Lee, Yun Kyu (Department of Acupuncture, Moxibustion, Meridian & Acupoint, Colleage of Oriental Medicine, Daegu Hanny University) ;
  • Lee, Bong Hyo (Department of Acupuncture, Moxibustion, Meridian & Acupoint, Colleage of Oriental Medicine, Daegu Hanny University) ;
  • Jung, Tae Young (Je-Han Oriental Medical Academy) ;
  • Kim, Jae Soo (Department of Acupuncture, Moxibustion, Meridian & Acupoint, Colleage of Oriental Medicine, Daegu Hanny University)
  • 신화영 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 이현종 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 임성철 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 이윤규 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 이봉효 (대구한의대학교 한의과대학 침구경혈학교실) ;
  • 정태영 (제한동의학술원) ;
  • 김재수 (대구한의대학교 한의과대학 침구경혈학교실)
  • Received : 2014.11.06
  • Accepted : 2014.12.01
  • Published : 2014.12.20
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Abstract

This study was performed to investigate the protective effects of acupuncture on the liver in the oxidative stress caused by cadmium accumulation. Sprague-Dawley male($150{\pm}30g$) rats were stabilized for a week and divided into 5 groups which is normal, control, $LR_3$ acupuncture, $BL_{23}$ acupuncture and sham acupuncture group. For three days experimental groups were received oral doses of cadmium 2 mg/kg twice a day. Acupuncture was given bilaterally at each point 10 times for two weeks. The depth of stimulation was 1 mm at right angles and torsion of acupuncture was produced 2 times per second for 1 minutes. The liver was shipped off and taken weight at the last day of two weeks, and hepatic functions was confirmed through alanine transaminase(ALT) and aspartate amino-transferase(AST). We measured reactive oxygen species of serum, liver and kidney, and compared expression levels of superoxide dismutase(SOD), catalase, glutathione peroxidase(Gpx), nuclear factor erythroid derived 2-related factor 2(Nrf-2), heme oxygenase-1(HO-1), nuclear factor-${\kappa}B$ (NF-${\kappa}B$), cyclooxygenase-2(COX-2), inducible nitric oxide synthase(iNOS), Bax and Cytochrome c. $BL_{23}$ acupuncture group significantly increased liver weight and decreased ALT compared to control group. For the oxidative stress, $LR_3$ acupuncture group significantly reduced reactive oxygen species, and $BL_{23}$ acupuncture group significantly reduced reactive oxygen species and inflammation-related protein compared to control group. But $LR_3$ acupuncture group and $BL_{23}$ acupuncture group didn't significantly reduce apoptosis-related protein. Therefore $LR_3$ and $BL_{23}$ acupuncture showed the effects of antioxidant and anti-inflammatory, especially $BL_{23}$ acupuncture was more effective than $LR_3$ acupuncture on the protection of liver in the oxidative stress.

Keywords

References

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