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Role of Diacyl Glycerol (DAG) in Caprine Sperm Acrosomal Exocytosis Induced by Progesterone

  • Somanath, P.R.;Gandhi, K.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.8
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    • pp.1091-1097
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    • 2002
  • Capacitated goat spermatozoa generated diacyl glycerol (DAG) when suspended in Krebs-Ringer bicarbonate medium and induced by progesterone or $Ca^{2+}$ ionophore A23187. We have added Sn-1-oleoyl-2-acetyl glycerol externally, to study the effect of DAG in goat sperm acrosomal exocytosis. Addition of neomycin abolished the DAG generating capacity of progesterone in a dose dependent manner, suggesting the involvement of a phosphoinositidase C activated phospholipase C system in the process. The level of increase in phosphatidic acid was considerably low and was produced well after the DAG generation thereby suggesting the involvement of a DAG kinase which phosphorylates DAG to produce PA. The inhibition of progesterone mediated effect by inhibitors of $GABA_A/Cl^{-}$ channel and $Ca^{2+}$ channels further supports the evidence that the events of binding of agonist to the receptor(s), opening of $Ca^{2+}$ channels and the activation of phospholipase C are reconciled to perform the function of acrosome reaction in capacitated goat spermatozoa.

Development of In vitro Technique for Bioavailable Corn Energy Value

  • Kim, I.B.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.11
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    • pp.1645-1646
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    • 2001
  • The objective of this study was to develop an in vitro digestion technique to estimate bioavailable energy values of different corn hybrids in swine and poultry. A total of 21 samples were investigated; 18 normal corn (NC) and 3 high-oil corn (HOC) hybrids. One step-two enzymes digestion system was introduced to develop the in vitro technique. The gross energy (GE) values of NC hybrids were correlated with the in vitro disappearing energy values (IVE; r=0.85, p<0.01), the in vitro energy digestibilities (IVED; r=0.70, p<0.01), and the in vitro DM digestibilities (IVDM; r=0.55, p<0.05). It appears, however, that IVE values of NC and HOC hybrids were not significantly different according to the one step-two enzyme digestion system. Results of in vivo and in vitro estimates suggested that there was no significant correlation between them in poultry. The IVE value was regressed linearly with ME and DE values in swine with low regression coefficient (34 and 41%, respectively).

Early Postmortem Processing Conditions on Meat Quality of Hanwoo (Korean Native Cattle) Beef during Storage

  • Kim, B.C.;Rhee, M.S.;Ryu, Y.C.;Imm, J.Y.;Koh, K.C.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.12
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    • pp.1763-1768
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    • 2001
  • The combined effects of low voltage electrical stimulation (ES) and early short-term temperature conditioning on meat quality of Hanwoo beef (Korean native cattle) during storage were investigated. Shear force was influenced by ES and aging. Combination of ES and the $30^{\circ}C$ conditioning resulted in higher myofibril fragmentation index and improved lightness. There was no substantial difference in drip loss among treatments but ES samples showed higher cooking loss than control. Negative effect on shelf-life was not found by early short-term high temperature conditioning. Therefore, the meat quality of Korean native cattle was effectively improved by the combination of ES and the $30^{\circ}C$ conditioning.

TREATMENT OF ANIMAL MANURE AND WASTES FOR ULTIMATE DISPOSAL - Review -

  • Winter, J.;Hilpert, R.;Schmitz, H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.5 no.2
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    • pp.199-215
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    • 1992
  • Sources of organic waste materials for aerobic and/or anaerobic degradation, or for composting of solid wastes in Germany were estimated. The basic microbiology and the energetics of these processes were compared with special emphasis on anaerobic degradation, for which a general degradation scheme of carbohydrates is presented. Advantages of anaerobic over aerobic treatment processes are pointed out and conditions for maintaining a highly stable anaerobic process as well as producing a sanitized, hygienic product are discussed. Reactor systems suitable for efficient treatment of wastes with a high or low proportion of suspended solids are principally compared and results of laboratory studies on the degradation of several wastes and animal manures summarized. Finally, a piggery slurry treatment factory for an ultimate slurry processing to obtain a dry fertilizer and a harmless, disposable liquid, as it is in operation in Helmond/Holland, is presented and preliminary process data are presented.

COMPARISON OF UTILIZATION OF CELLULOSE AND CORN DIETARY FIBER AS AN ENERGY SOURCE IN CHICKS

  • Muramatsu, T.;Morishita, T.;Furuse, M.;Okumura, J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.5 no.1
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    • pp.151-157
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    • 1992
  • An experiment was conducted to investigate effects of fiber source on growth performance, N and neutral detergent fiber (NDF) digestibility, and utilization of energy in chicks fed an isocaloric low-energy diet from 7 to 21 days of age. Two fiber sources, cellulose and corn dietary fiber (CDF), were included in a diet at 10, 20 and 30% at the expense of kaolin, an inert diluent. The CDF contained 76.5% NDF consisting mainly of hemicellulose. The results showed that growth performance, N and NDF digestibility, dietary DE and ME values, energy deposition, and NE for production in birds fed CDF were inferior to those in birds fed cellulose. It can be concluded, from the present study, that chicks can utilize cellulose more efficiently than CDF up to a level of 30%.

Understanding Starch Utilization in the Small Intestine of Cattle

  • Harmon, David L.
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.7
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    • pp.915-922
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    • 2009
  • Ruminants possess the capacity to digest very large amounts of starch. However, in many cases diets approach 60% starch and even small inefficiencies present opportunities for energetic losses. Ruminal starch digestion is typically 75-80% of starch intake. On average, 35-60% of starch entering the small intestine is degraded. Of the fraction that escapes small-intestinal digestion, 35-50% is degraded in the large intestine. The low digestibility in the large intestine and the inability to reclaim microbial cells imposes a large toll on post-ruminal digestive efficiency. Therefore, digestibility in the small intestine must be optimized. The process of starch assimilation in the ruminant is complex and remains an avenue by which increases in production efficiency can be gained. A more thorough description of these processes is needed before we can accurately predict digestion occurring in the small intestine and formulate diets to optimize site of starch digestion.

Consumers' Awareness of the Risk Elements Associated with Foods and Information Search Behavior Regarding Food Safety (소비자의 식품 위해요인에 대한 인식도 및 식품 안전에 대한 정보탐색 행동)

  • Kim, Hyo-Chung;Kim, Mee-Ra
    • Journal of the East Asian Society of Dietary Life
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    • v.19 no.1
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    • pp.116-129
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    • 2009
  • The study was conducted to evaluate consumer awareness of the risk elements associated with foods and their information search behavior regarding food safety. The data were collected from 504 adult consumers living in Seoul, Busan, Incheon, Daegu, Daejeon, and Gwangju through a self-administered questionnaire on August, 2006. Frequency analyses, t tests, one-way analysis of variance, and Duncan's multiple range comparison tests were conducted to analyze the responses using SPSS v. 14.0. The levels of recognition of consumers regarding each risk element were generally low. Many respondents answered that they obtained information regarding food safety from TV/radio/newspapers and family/relatives/friends/neighbors. The respondents also indicated that they had strong confidence in the information from family/relatives/friends/neighbors. Additionally, most respondents required information regarding heavy metal contamination, endoctrine disruptors, and avian influenza.

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Preparation and Chemical Characteristics of Food Protein Hydrolysates (식품단백질 효소분해물의 제조 및 이화학적 특성)

  • Kim, Jong-Hee;Hong, Soon-Kwang
    • Journal of the East Asian Society of Dietary Life
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    • v.19 no.1
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    • pp.45-51
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    • 2009
  • In this study, food protein hydrolysates were prepared from six types of food protein: purified meat protein, whole egg protein, casein, isolated soy protein, concentrated rice protein, and gluten. Food proteins were hydrolyzed with pepsin and ethanol (80%)-soluble fractions of pepsin hydrolysates were employed for analysis. The products were colorless and odorless powders with low fat content and good solubility. The MW (molecular weight) of the protein hydrolysates was confirmed to be $200{\sim}1,800$ via gel filtration. Free amino acid contents accounted for less than 5% of the samples. The results of our amino acid analysis revealed that all food protein hydrolysates preserved their original amino acid compositions and nutritional values of their source proteins with highly pure oligopeptide mixtures. These results show that the food protein hydrolysates prepared in these investigations should prove excellent dietary nitrogen sources for a variety of applications.

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Quality Characteristics of Snack with Added Dongchimi Powder (동치미 분말을 이용한 스낵의 품질 특성)

  • Choi, Jin Joo;Park, Yoo Ri;Chung, Chang-Ho
    • Journal of the East Asian Society of Dietary Life
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    • v.24 no.6
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    • pp.819-826
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    • 2014
  • Consumer's demand for higher dietary fiber and lower fat in processed foods are increasing. This study developed a low fat and high dietary fiber snack using egg whites and dry fermented radish powder obtained from Dongchimi fermentation. Quality characteristics, such as hardness, color, fat content and total dietary fiber, of the developed snack were conducted along with a hedonic test. As the amount of the fermented powder increased in the formulation, water content and snack hardness decreased. Up to 14.5% radish powder was added (or 9.54% dietary fiber; dry weight, w/w) to the formulation, and hedonic test found snack containing 4.8% (w/w) powder showed the highest scores for overall acceptability.

Research Productivity of Graduate Students in GPF program of Korea

  • Kim, Ki-Hyoung
    • Asian Journal of Innovation and Policy
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    • v.3 no.1
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    • pp.72-93
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    • 2014
  • This study analyzed the factors that influence the research productivity of 236 graduate students who are funded by the Global Ph.D. Fellowship Program of the National Research Foundation of Korea. Research productivity was measured by the number of SCI publications, and the explanatory factors are the demographic factor, the financial factor, the reputation of institutions and the supervisor factor. This study included 2 indices such as the reputation of institutions and the research productivity of supervisors to check the halo effect unlike other studies. Results are as follows: 1) no gender difference, 2) better performance by younger age group, 3) no difference even if the students are additionally funded by other research support programs, 4) no halo effect by the reputation of institution but rather better performance from low ranked universities, 5) and a positive halo effect by supervisors.