• Title/Summary/Keyword: artificial seedling

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Effects of Simulated Acid Rain on Growth and Contents of Chemical Substances in Needles of Pinus koraiensis Seedlings and on Chemical Properties of the Tested Soil (인공산성우(人工酸性雨)가 잣나무 유묘(幼苗)의 생장(生長), 엽내함유성분(葉內含有成分) 및 토양(土壤)의 화학적(化學的) 성질(性質)에 미치는 영향(影響))

  • Cheong, Yong Moon
    • Journal of Korean Society of Forest Science
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    • v.76 no.1
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    • pp.33-40
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    • 1987
  • Simulated acid rain (pH 4.0, pH 2.0) containing sulfuric and nitric acid in the ratio of 60:40 (chemical equivalent basis) diluted with underground water, and underground water (pH 6.5) as control were treated on potted Pines koraiensis seeds during the growing season (May 1 to August 31) in 1985. The regime of artificial acid rain, in terms of spray frequency and amount per plot, was simulated on the basis of climatological data averaged for 30 years of records. The seedling growth, contents of chemical substances in needles and chemical properties of the tested soil were compared among the various pH levels of acid rain on October 31, 1985. Following results were obtained. 1. With decreasing pH levels of acid rain, S and $K_2O$ contents in leaf tissue were increased, but MgO and $P_2O_5$ contents were decreased. 2. Soil pH was dropped, and exchangeable aluminum content in the tested soil was dramatically increased as the pH levels of acid rain decreased. 3. Exchangeable calcium, magnesium, potassium contents, and base saturation degree of the soil were significantly decreased with decreasing pH levels of acid rain. 4. Sulfate concentrations in the soil were significantly increased as rain pH decreased, but total nitrogen and available phosphate contents were not influenced by acid rain.

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Studies on the Artificial Seedling Production of Geoduck Clam, Panope japonica II. Development of Egg and larvae (코끼리조개의 인공종묘생산에 관한 연구 II. 난발생과 유생의 발달)

  • Lee, Chae-Sung;Rho, Sum
    • Journal of Aquaculture
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    • v.10 no.1
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    • pp.25-32
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    • 1997
  • Develoment precess and characteristics of eggs of the geoduck clam, Panope japonica are reporting in this study. Eggs and sperm were excised from gonad, artificially fertilized in an aquarium, reared under various temperature regimes, and record and record the larval period and the time need to reach a certain larval stage from ferilization. Unfertilized eggs of P. japonica appeared to be oval with a mean diameter of $70\mu$m and they became spherical after fertilization. The eggs of P. japonica can be classified as demersal. At a constant water temperature of $ 11^{citc}C$, it took 4 hours form fertilization to become four-cell stage, two days to become trochophore larvae, three days to become D-shape larvae, twenty-three days to become umbo stage, and thirty-six days to become fully grown veliger ready form settlement. A negative correlation was observed between the water temperature and the larval period of P. japonica. From fertilization to D-shape larvae, it took five days at 8$^{\circ}C$, while it was only two days to become D-shape larvae at $ 17^{citc}C$. Time required to D-shape larvae from fertilization was proportional to temperature, and the relationships were expressed as follows : To 8-cell stage, 1/t=0.0209 w-0.1167 (r=0.9967) To blastula stage, 1/t=0, 0055 w-0.0192 (r=0.9825) To trochophore stage, 1/t=0.0034 w-0.0155 (r=0.9907) To D-shape larvae stage, 1/t=0.0014 w-0.0023 (r=0.9843) (t, time in hours ; w, water temperature) Bioligical minimum temperature for egg development was calculated as 3.82$^{\circ}C$ in average.

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Characteristics of mulberry cultivar 'palchung' (Morus Lhou(Ser.) Koidz.) for mulberry breeding as pollen parent (오디 생산용 뽕 품종 육성을 위한 교배모본 '팔청'의 특성)

  • Sung, Gyoo Byung;Kim, Yong Soon;Kim, Kee Young;Kim, Hyun-Bok;Ji, Sang;Kang, Pil Don
    • Journal of Sericultural and Entomological Science
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    • v.52 no.2
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    • pp.147-154
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    • 2014
  • We bred a mulberry cultivar named Palcheong, through local adaptability test, which is under registration as a new cultivar for fruit production. Local adaptability test had been carried out at four places (Suwon, Kongju, Wanju, and Jinju) for seven years from 2007. This is belonging to (Morus Lhou(Ser.) Koidz.) selected among mulberry genetic resources preserved in Suwon. Its sex expression was hermaphrodite, which can be used for the production of seedling for fruit production by artificial crossing as pollen parent Palchung showed lower yielding in fruit productivity by 16% compared to control cultivar 'Chungil (Morus alba L.)' for five years. Although Palchung was higher in sugar content of mulberry fruits, it was bigger than that of 'Chungil'. It is adaptable to every where except the places where cold damage and sclerotic disease happen frequently.

'Picnic', a New Mid-season Apple Cultivar with Medium Size and Good Taste (식미가 우수한 중과형 사과 '피크닉' 육성)

  • Kwon, Soon-Il;Park, Jong-Taek;Lee, Jung-Woo;Kim, Mok-Jong;Kim, Jeong-Hee
    • Horticultural Science & Technology
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    • v.33 no.5
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    • pp.784-788
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    • 2015
  • A new cultivar 'Picnic' originated from an artificial cross between 'Fuji' and 'Sansa' at National Institute of Horticultural & Herbal Science in 1994. The cultivar was preliminarily selected among the elite siblings for its high fruit quality in 2003. After regional adaptability tests in five districts for four subsequent years as 'Wonkyo Ga-34', it was ultimately selected in 2008. Optimum harvest time is late September. Mature fruit has mean weight of 233 g and is conic with light red skin on a greenish yellow ground and yellowish white flesh. The fruit contains a favorable total soluble solids content at $13.8^{\circ}Brix$ and titratable acidity at 0.43%, which results in gustatory harmony between sugars and acids. It is not resistant to bitter rot or Marssonina blotch. 'Picnic' exhibits a physiological cross compatibility with leading cultivars such as 'Fuji', 'Hongro' and 'Tsugaru'. Tree topology is semi-spreading with a weak growth habit.

'Arihyang', a Strawberry Variety with Highly Firm and Large-Sized Fruit for Forcing Culture (촉성재배용 고경도 대과성 딸기 품종 '아리향')

  • Kim, Dae-Young;Kim, Seung Yu;Huh, Yun-Chan;Yoon, Moo Kyung;Lee, Sun Yi;Moon, Ji-Hye;Kim, Dae Hyun
    • Korean Journal of Breeding Science
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    • v.50 no.4
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    • pp.497-503
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    • 2018
  • A strawberry variety 'Arihyang' was derived as an artificial cross between 'Tochiotome' and 'Seolhyang' in 2014. The seedling and line selections were conducted from 2014 to 2015. Preliminary and advanced yield trials of '14-5-5,' which was the final selected line, were conducted from 2015 to 2017. 'Arihyang' is suitable for forced cultivation and has strong plant vigor, uniformly large-sized fruit, and a high yield compared to those of the check variety, 'Seolhyang' and 'Maehyang.' Especially, vitamin C was at a significant level, which was approximately 15% higher than that of 'Seolhyang.' The average number of flowers per first flower cluster was 10.5, which could reduce the labor of thinning fruit. Its fruit has a conical shape, dark red color, and glossy skin. The fruit was of good quality but has recommendations for harvest at the fully ripened stage. 'Arihyang' has intermediate resistant to phytophthora crown rot, but is susceptible to powdery mildew, gray mold, anthracnose, and fusarium wilt. It is reguired to manage major diseases and pests using optimum cultivation techniques and chemical control.

Status of Fusarium Wilt Incidence on Summer Radish and Etiological Characteristics of the Causal Fungus in Korea (고랭지 여름 무에서 시들음병 발생 현황과 병원균의 병원학적 특성 연구)

  • Hong, Sung Kee;Ko, Hyoungrai;Choi, Hyo-Won;Lee, Youngkee;Kim, Jeomsoon
    • Research in Plant Disease
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    • v.26 no.4
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    • pp.256-263
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    • 2020
  • Incidence of Fusarium wilt was surveyed in fields of summer radish in Gangwon province in Korea in 2018 and 2019. The disease started in early July and spread rapidly in hot summer of late July and August and in severe case, reached up to 80% in a field in Gangneung area. Symptoms in the seedling stage include poor growth and browning of internal tissue of root. During mid-growth, the leaves of diseased plant turned yellow over time, the surface of the roots changed from white to blackish, and the vascular tissues turned brown. A total of 23 isolates was obtained from the diseased plants and identified as Fusarium oxysporum f. sp. raphani by elongation factor-1α and intergenic spacer sequence analysis. Pathogenicity of the isolates was tested by artificial inoculation to the radish and other plants. All the isolates tested were pathogenic to radish plant, although there were differences in virulence on radish 11 cultivars. However, the isolates were not virulent to other plants except some cruciferous vegetables including Brussels sprouts, rocket, stock, and turnip. The results of pathogenicity test showed that it is necessary to rotate with crops other than cruciferous vegetables in order to prevent Fusarium wilt from radish fields.

Hydroponic Nutrient Solution and Light Quality Influence on Lettuce (Lactuca sativa L.) Growth from the Artificial Light Type of Plant Factory System (인공광 식물공장에서 수경배양액 및 광질 조절이 상추 실생묘 생장에 미치는 영향)

  • Heo, Jeong-Wook;Park, Kyeong-Hun;Hong, Seung-Gil;Lee, Jae-Su;Baek, Jeong-Hyun
    • Korean Journal of Environmental Agriculture
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    • v.38 no.4
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    • pp.225-236
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    • 2019
  • BACKGROUND: Hydroponics is one of the methods for evaluating plant production using the inorganic nutrient solutions, which is applied under the artificial light conditions of plant factory system. However, the application of the conventional inorganic nutrients for hydroponics caused several environmental problems: waste from culture mediums and high nitrate concentration in plants. Organic nutrients are generally irrigated as a supplementary fertilizer for plant growth promotion under field or greenhouse conditions. Hydroponic culture using organic nutrients derived from the agricultural by-products such as dumped stems, leaves or immature fruits is rarely considered in plant factory system. Effect of organic or conventional inorganic nutrient solutions on the growth and nutrient absorption pattern of green and red leaf lettuces was investigated in this experiment under fluorescent lamps (FL) and mixture Light-Emitting Diodes (LEDs). METHODS AND RESULTS: Single solution of tomatoes (TJ) and kales (K) deriving from agricultural by-products including leaves or stems and its mixed solution (mixture ration 1:1) with conventional inorganic Yamazaki (Y) were supplied for hydroponics under the plant factory system. The Yamazaki solution was considered as a control. 'Jeockchima' and 'Cheongchima' lettuce seedlings (Lactuca sativa L.) were used as plant materials. The seedlings which developed 2~3 true leaves were grown under the light qualities of FL and mixed LED lights of blue plus red plus white of 1:2:1 mixture in energy ratio for 35 days. Light intensity of the light sources was controlled at 180 μmol/㎡/s on the culture bed. The single and mixture nutrient solutions of organic and/or inorganic components which controlled at 1.5 dS/m EC and 5.8 pH were regularly irrigated by the deep flow technique (DFT) system on the culture gutters. Number of unfolded leaves of the seedlings grown under the single or mixed nutrient solutions were significantly increased compared to the conventional Y treatment. Leaf extension of 'Jeockchima' under the mixture LED radiation condition was not affected by Y and YK or YTJ mixture treatments. SPAD value in 'Jeockchima' leaves exposed by FL under the YK mixture medium was approximately 45 % higher than under conventional Y treatment. Otherwise, the maximum SPAD value in the leaves of 'Cheongchima' seedlings was shown in YK treatment under the mixture LED lights. NO3-N contents in Y treatment treated with inorganic nutrient at the end of the experiment were up to 75% declined rather than increased over 60 % in the K and TJ organic treatment. CONCLUSION: Growth of the seedlings was affected by the mixture treatments of the organic and inorganic solutions, although similar or lower dry weight was recorded than in the inorganic treatment Y under the plant factory system. Treatment Y containing the highest NO3-N content among the considered nutrients influenced growth increment of the seedlings comparing to the other nutrients. However effect of the higher NO3-N content in the seedling growth was different according to the light qualities considered in the experiment as shown in leaf expansion, pigmentation or dry weight promotion under the single or mixed nutrients.

Effects of Size and Environmental Condition on Burrowing of Artificial Seedling of Ark Shell, Scapharca broughtonii (Schrenck) (피조개, Scapharca broughtonii (Schrenck) 인공치패의 크기 및 환경조건이 잠입에 미치는 영향)

  • Kim, Byeong-Hak;Shin, Yun-Kyung;Choi, Nack-Joong;Oh, Bong-Se;Sohn, Sang-Gyu;Jung, Choon-Goo;Son, Tai-Sun;Kang, Kyoung-Ho
    • The Korean Journal of Malacology
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    • v.23 no.1
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    • pp.1-8
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    • 2007
  • The influence of individual size, sediment, gain size, water temperature, salinity and air exposure on burrowing rate was investigated in order to obtain the basic biological data on applying shellfish farm for a sustainable production of ark shell, Scapharca broughtonii (Schrenk). The burrowing rate on individual size 300 minutes after starting the experiment was the highest in the shell length $16.3\;{\pm}\;1.2\;mm$, 97.7%. The highest burrowing rates were 97.0% in $12.8\;{\pm}\;0.8\;mm$, 96.7% in $9.2\;{\pm}\;1.0\;mm$, and 96.3% in $5.9\;{\pm}\;0.7\;mm$. The clams over 6 mm of shell length had burrowing ability and the burrowing rate was not related to the shell size. The burrowing rate depending on the kind of grain at the bottom after 300 minutes was the highest, 98.3%, in the mixture of sand and silt with a ratio of 75:25. The rates were 98% in silt (100%), 97.3% in mixture sand and silt with a ratio of 50:50, 97.3% in sand and silt ratio of 25:75, and 86.3% in sand (100%) in this specific order. On grain size of the soil in the seafloor, the burrowing rates after 300 minutes was at its highest in the group of sand in pore size 1 mm with 85.0%, and the $12\;{\mu}m$ to 1 mm in the grain size was fitted to burrowing of artificial seed. In the case of water temperature, the burrowing rates were at its highest after 300 minutes. In $30^{\circ}C$ group, the rate was 96.7% and in $25^{\circ}C$ and $20^{\circ}C$, 90.0%. The rates decreased as the water temperature decreased below $15^{\circ}C$. The burrowing rates on salinity were the highest in 30 psu with 93.3% and at 15 psu and below, there was no noticeable change in the burrowing rate. On air exposure, the burrowing rates after 300 minutes were the highest in 1 hour with 93.3%, and remarkably decreased as air exposure time is longer after 12 hours of air exposure.

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Response of the Growth Characteristics and Phytochemical Contents of Pepper (Capsicum annuum L.) Seedlings with Supplemental LED Light in Glass House (LED 보광처리가 고추(Capsicum annuum) 묘의 생장과 Phytochemical 함량에 미치는 영향)

  • Azad, Md. Obyedul Kalam;Chun, Ik-Jo;Jeong, Jeong-Hak;Kwon, Soon-Tae;Hwang, Jae-Moon
    • Journal of Bio-Environment Control
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    • v.20 no.3
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    • pp.182-188
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    • 2011
  • This research was conducted to evaluate the effect of supplemental light-emitting diode (LED) light on growth characteristics and phytochemical content of pepper (Capsicum annuum L.) seedling using LED blue (470 nm, B), red (660 nm, R), blue + red (BR), far red (740 nm, FR) and UV-B (300 nm) light treatment, and without artificial light. Photon flux of LED light was 49, 16, 40, 5.0 and $0.82{\mu}mol\;m^{-2}s^{-1}$ for B, R, BR, FR, and UV-B light, respectively, during experiment. Supplemental LED light duration was $16hr\;day^{-1}$ and UV-B light duration was 10 min. per day after sunset up to 15 days (12 days after germination) of plants age. In our research, growth characteristics and phytochemical content of pepper seedlings were greatly influenced by supplemental LED light compare to control treatment. Red light increased the number of leaves, number of nodes, leaf width and plant fresh weight by 34%, 27%, 50% and 40%, respectively. Blue light increased the leaf length by 13%, and stem length and length of inter node were increased by 17% and 34%, respectively under grown far red light. After 15 days of light treatments phytochemical concentrations of pepper plants were significantly changed. Blue light enhanced the total anthocyanin and chlorophyll concentration by 6 times and 2 times, respectively. Red light increased the total phenolic compound at least two folds meanwhile far red light reduced the ascorbic acid and antioxidant activity 31% and 66%, respectively compared to control treatment.

Identification of Feeding Stimulants for Juvenile Pacific Bluefin Tuna, Thunnus orientalis in Muscle Extract of Horse Mackerel, Trachurus japonicus (참다랑어(Thunnus orientalis) 치어에 있어서 전갱이(Trachrus japonicus) 근육 엑기스 중의 섭이촉진물질 검색)

  • Ji, Seung-Cheol;Takaoka, Osamu;Seoka, Manabu;Kohbara, Jun;Hosokawa, Hidetuyo;Shimeno, Sadao;Jeong, Gwan-Sik;Lee, Si-Woo;Takii, Kenji
    • Journal of Aquaculture
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    • v.20 no.1
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    • pp.19-25
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    • 2007
  • For establishing a basal diet for the Pacific bluefin tuna Thunnus orientalis (PBT), feeding stimulants were initially identified by omission test using the synthetic extract of horse mackerel, Trachurus japonicus. Four feeding trials were conducted using juvenile PBT weighing $9.0{\pm}0.91\;g$ (trial 1, 2 and 3) and $1.6{\pm}0.23\;g$ (trial 4), which were originated from an artificial seedling production. The fish fed the casein diet with each test solution were added at the ratio of 100 g casein diet to 100 g jack mackerel muscle. A complete synthetic extract of jack mackerel containing all 3 fractions, amino acid, nucleotide and organic nitrogenous base, exhibited a comparable feeding stimulant activity compared to that of natural extract. The omission of nucleotide or amino acid fraction showed lower feeding activity, but the omission of other nitrogenous fraction maintained a similar feeding stimulant activity compared to that of the synthetic extract (trial 1). Inosine-5' monophosphate $Na_2$ (IMP) was identified as a major constituent for maintaining feeding activity. The mixture of L-alanine, L-glutamic acid, L-histidine, L-lysine, taurine and IMP induced a similar feeding activity compared to that of the synthetic extract (trial 2 and 3). In trial 4, the highest feeding activity was finally obtained in the mixture of L-histidine, L-glutamine and IMP, followed by the synthetic extract, the mixture of L-lysine, L-alanine and IMP, IMP and the mixture of L-histidine, L-glutamic acid, L-lysine and L-alanine. These results revealed that the mixture of L-histidine, L-glutamic acid and IMP for the proper feeding stimulant of PBT in this study.