• 한국균학회지
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• 제28권2호
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• pp.73-80
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• 2000

공시배지 중 MYPA 배지에서 큰느타리의 균사생장 및 밀도가 가장 양호하였으며, 균사생장 최적온도는 $25^{\circ}C$이고, 최적 pH는 6.0이었다. 최적 회전진탕배양 일수는 7일이었고, 큰느타리의 균사생장을 위한 최적배지조성은 탄소원 soluble starch 3%(w/v), 질소원 malt extract 0.25%(w/v), yeast extract 0.25%(w/v), 무기염류 $CaCl_2{\cdot}2H_2O$ 0.05%(w/v)이었다. 기본배지인 MYP 배지와 최적 배지로 액체배양하여 균체량을 비교한 결과, 최적배지에서 균체생산량 및 생장수율이 높았다. 큰느타리의 균사생장과 균밀도에 최적인 톱밥수종은 참나무 톱밥이었고, 첨가제는 미강과 밀기울 30%(v/v)이었으며, 최적 수분함량은 70%(v/v)이었다. 반면에 자실체 생산을 위한 최적 첨가제는 발겨 20%(v/v)이었다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2003년도 제10차 국제학술회의 및 추계정기 학술발표회
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• pp.2-3
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• 2003

A method for rapid propagation of mature Jack fruit was developed. Four types of explants (mature embryos, apical meristems of young seedlings, apices from mature plants and nodal segments) were used. It has been found 88% of young apical meristems produced shoots in Campbell and Durzan (CD) medium compared to 60% in Murashige and Skoog (MS) medium. Only 1/3 of them produced multiple shoots. Shoot initiation from nodal segments was very rare. Mature apices produced callus. Although removal of the sheathing cover around mature buds enhanced the shoot initiation but success rate was low in growth regulator free medium. Embryos respond to the CD medium but not to the MS medium. Embryos from seeds soaked in water for 24 hours produced shoots after 8 weeks of incubation and the success rate was 70% while embryos from dry seeds only produced roots. There was no significant effect of cold storage (refrigeration) for 7 days on shoot initiation from mature embryos (65%) but the ability for shoot induction declines with storage time (55% after 21 days of cold storage). Mature axillary buds were established in Modified Campbell and Durzan (CD) medium supplemented with 0.5mg/1 and IBA. There was a significant difference in the growth performance of shoots according to the period of the year in which explants were collected. Highest (60%) was observed in November-January period. It was only 30% when the explants were collected in February-April or May-July and decreased to 20% in August-October. The shoots produced in November-January showed a higher vigor than those produced in other months. Since Jak fruit show seasonal changes in fruit bearing and shedding of leaves, it can be suggested that the difference in growth performances of tissues cultured in artificial culture media would have been affected by endogenous rhythms.

• Journal of Plant Biotechnology
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• 제33권4호
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• pp.243-248
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• 2006

A optimal procedure for plant production via repetitive secondary somatic embryogenesis in Tilia amurensis is described. Somatic embryos were induced directly from the culture of zygotic embryos on medium with 1.0 mg/l 2,4.-D. Repetitive secondary somatic embryos formed on the surface of the cotyledons and hypocotyls except for the radicles when explants of somatic embryos were cultured on medium with 4.0 mg/l 2,4-D. The highest frequency of secondary embryo-genesis was obtained in the cotyledons (90%) and hypocotyls(83.33%) on MS medium with 1.0 mg/L 2,4-D. The average number of secondary embryos per explant was 25.74 in cotyledon and 24.92 in hypocotyl. When the cotyledon and hypocotyl segments from somatic embryos at different developmental stages were cultured on MS medium with 1.0 mg/L 2,4-D, the highest frequency of secondary embryogenesis was obtained from late cotyledonary secondary embryos. Somatic embryos were transferred to MS basal medium and then they germinated within 2 to 4 weeks of culture. Germinated somatic embryos grew normally into plantlets on WPM medium, producing new shoots. The converted plantlets were acclimatized on artificial soil mixture. These results indicate that the repetitive secondary somatic embryogenesis in T amurensis can offer the possibility to use in vitro culture system for the micropropagation.

• Plant Biotechnology Reports
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• 제3권4호
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• pp.277-283
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• 2009

One of the limitations to conducting maize Agrobacterium-mediated transformation using explants of immature zygotic embryos routinely is the availability of the explants. To produce immature embryos routinely and continuously requires a well-equipped greenhouse and laborious artificial pollination. To overcome this limitation, an Agrobacterium-mediated transformation system using explants of type II embryogenic calli was developed. Once the type II embryogenic calli are produced, they can be subcultured and/or proliferated conveniently. The objectives of this study were to demonstrate a stable Agrobacterium-mediated transformation of maize using explants of type II embryonic calli and to evaluate the efficiency of the protocol in order to develop herbicide-resistant maize. The type II embryogenic calli were inoculated with Agrobacterium tumefaciens strain C58C1 carrying binary vector pTF102, and then were subsequently cultured on the following media: co-cultivation medium for 1 day, delay medium for 7 days, selection medium for $4{\times}14$ days, regeneration medium, and finally on germination medium. The T-DNA of the vector carried two cassettes (Ubi promoter-EPSPs ORF-nos and 35S promoter-bar ORF-nos). The EPSPs conferred resistance to glyphosate and bar conferred resistance to phosphinothricin. The confirmation of stable transformation and the efficiency of transformation was based on the resistance to phosphinothricin indicated by the growth of putative transgenic calli on selection medium amended with $4mg\;1^{-1}$ phosphinothricin, northern blot analysis of bar gene, and leaf painting assay for detection of bar gene-based herbicide resistance. Northern blot analysis and leaf painting assay confirmed the expression of bar transgenes in the $R_1$ generation. The average transformation efficiency was 0.60%. Based on northern blot analysis and leaf painting assay, line 31 was selected as an elite line of maize resistant to herbicide.

• 한국IT서비스학회지
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• 제20권1호
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• pp.131-143
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• 2021

Commercial transactions, one of the pillars of the capitalist economy, are occurring countless times every day, especially small and medium-sized businesses. However, small and medium-sized enterprises are bound to be the legal underdogs in contracts for commercial transactions and do not receive legal support for contracts for fair and legitimate commercial transactions. When subcontracting contracts are concluded among small and medium-sized enterprises, 58.2% of them do not apply standard contracts and sign contracts that have not undergone legal review. In order to support small and medium-sized enterprises' fair and legitimate contracts, small and medium-sized enterprises can be protected from legal threats if they can reduce the risk of signing contracts by analyzing various risks in the contract and analyzing and informing them of toxic clauses and omitted contracts in advance. We propose a risk prediction model for the machine reading-based legal contract to minimize legal damage to small and medium-sized business owners in the legal blind spots. We have established our own set of legal questions and answers based on the legal data disclosed for the purpose of building a model specialized in legal contracts. Quantitative verification was carried out through indicators such as EM and F1 Score by applying pine tuning and hostile learning to pre-learned machine reading models. The highest F1 score was 87.93, with an EM value of 72.41.

• Current Research on Agriculture and Life Sciences
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• 제33권1호
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• pp.7-14
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• 2015

This study identifies the complementarity or substitutability among apple production technology bundles. To identify the production technology bundle relationships, this paper focuses on the 8 most commonly used technology groups, including a high density plant, normal density plant, M9, M26, more than 70% adoption ratio of a medium-maturing variety, the adoption of a new variety, artificial pollination, and low-temperature storage. The results show that M9 has a complementary relationship with a high density plant, yet a substitute relationship with a normal density plant. Meanwhile, M26 has a complementary relationship with both a high and normal density plant. No substitute relationship is found between M9 and M26, yet a complementary relationship is identified between the adoption of artificial pollination and low-temperature storage and M9 or M26.

• 한국임상수의학회지
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• 제17권2호
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• pp.420-430
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• 2000

These studies were preformed to investigate the freezing conditions to achieve good post-thaw viability of spend and the practical methods of artificial insemination frozen canine semen. Semen were collected from nine male dogs which had been proved to be fertile in the past and the semen were treated for freezing procedure. Post-thaw motility and viability of canine sperm were evaluated to investigate individual tolerance of freezing, difference among freezing extenders, dif-ference among freezing equipments and freezing conditions, difference between fast and slow cooling rate, difference according to different glycerol concentration, effect of seeding on post-thaw viability, difference according to cutting part of straw, difference according to thawing temperatures, and dif-ference according to media added to thawed semen. Thawed semen for insemination were added with equal volnme of canine capacitation medium (CCM) and the volume of semen and the number per insemination were adjusted as 2-3 ml and $20-30 {\times}10^7,$ respectively. The semen were inseminated in vagina using balloon catheter and en17ryos were cellected from 9 to 11 days after the second Al to d determine fertilization.

• Journal of Microbiology and Biotechnology
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• 제4권4호
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• pp.270-273
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• 1994

Pseudomonas putida 3SK, which was constructed by the conjugal transfet of the $CAM::TOL^{*}$ plasmid of Pseudomonas putida CSnA and the NAH plasmid of Pseudomonas putida KCTC 2403 into n-alkane assimilating Pseudomonas putida KCTC 2405, showed a broad degradation spectrum and floc-forming ability. This strain degraded m-toluic acid, naphthalene, camphor and decane simultaneously. $Hg^{2+}$ at the concentration of 1 ppm in the minimal medium could not inhibit the growth of this strain. The degradation of m-toluic acid by Pseudomonas putida 3SK was not repressed by the easily utilizable compounds, such as glucose and succinate. But, the addition of formalin inhibited the growth of Pseudomonas putida 3SK. After the cultivation of this strain on the artificial wastewater containing m-toluic acid, naphthalene, camphor and decane for 24 hr, the initial COD value (1500) of the artificial wastewater was declined to 300.

• 예술경영연구
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• 제59호
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• pp.5-31
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• 2021

본 연구는 인공지능 시대의 미술창작에 관한 사회적 인식 및 주요 담론을 분석하여, 인공지능 등장에 따른 예술계의 대응 방안을 모색하는 것에 그 목적이 있다. 이에 본 논문은 인공지능을 통한 창작원리와 한계를 개념적으로 이해하고, 예술사회학적 관점을 바탕으로 인공지능 미술창작을 사회적 맥락에서 해석했다. 본고는 인공지능 미술창작 관련 기사 472건을 주요 자료로 삼고 언어 네트워크 분석을 진행하였다. 연구결과, 인공지능 미술창작의 주체에 대한 혼재된 관점이 언어 네트워크상에서 나타났다. 그러나 지식재산권의 인정을 표상하는 단어군집의 지배적 영향력을 미루어보아, 인공지능을 미술창작의 주체로서 간주하는 관점 중심으로 사회적 인식이 형성됨을 포착하였다. 또한 해당 군집과 제도적 지원을 반영하는 군집의 밀접한 관계를 바탕으로 인공지능 미술에 대한 핵심 담론이 기술 발전과 법적 체제 정비에 한정되어 있음을 확인하였다. 이에, 본 연구는 매체로서의 인공지능의 규정 및 장르로서의 인공지능 미술에 대한 정책적 담론 형성의 필요성을 시사한다.

• 한국국방경영분석학회지
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• 제37권1호
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• pp.1-9
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• 2011

현대 국방획득 업무에서 계약업체의 관리는 중요한 요소라고 할 수 있다. 국방조달에 있어서 부정당업자의 발생은 무기체계의 전력화에 큰 차질을 유발시켜 획득사업이 원활히 이행되지 못하는 원인을 야기시킨다. 본 논문에서는 인공신경망 모형을 이용하여 국방조달에서 부정당업자를 판별할 수 있는 예측모형을 개발하고자 한다. 모형에 사용된 데이터는 국내 중소 제조기업을 대상으로 구축된 학습용 및 검증용 자료를 활용하여 실증분석 하였다. 본 연구를 통해 제시된 모형의 결과로 사업관리자들의 사업관리 능력을 향상시키고, 견실한 국방조달업체의 진입을 통한 조달질서 확립에 기여를 할 수 있으리라 기대된다.