Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Micropropagation of Tilia amurensis via Repetitive Secondary Somatic Embryogenesis

  • Kim, Tae-Dong (Forest Seed Research Center, Korea Forest Research Institute) ;
  • Choi, Yong-Eui (Division of Forest Resources, College of Forest an Environmental Sciences, Kangwon National University) ;
  • Lee, Byoung-Sil (Forest Seed Research Center, Korea Forest Research Institute) ;
  • Kim, Young-Joung (Forest Seed Research Center, Korea Forest Research Institute) ;
  • Kim, Tae-Su (Forest Seed Research Center, Korea Forest Research Institute) ;
  • Kim, In-Sik (Forest Seed Research Center, Korea Forest Research Institute)
  • Published : 2006.12.30
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Abstract

A optimal procedure for plant production via repetitive secondary somatic embryogenesis in Tilia amurensis is described. Somatic embryos were induced directly from the culture of zygotic embryos on medium with 1.0 mg/l 2,4.-D. Repetitive secondary somatic embryos formed on the surface of the cotyledons and hypocotyls except for the radicles when explants of somatic embryos were cultured on medium with 4.0 mg/l 2,4-D. The highest frequency of secondary embryo-genesis was obtained in the cotyledons (90%) and hypocotyls(83.33%) on MS medium with 1.0 mg/L 2,4-D. The average number of secondary embryos per explant was 25.74 in cotyledon and 24.92 in hypocotyl. When the cotyledon and hypocotyl segments from somatic embryos at different developmental stages were cultured on MS medium with 1.0 mg/L 2,4-D, the highest frequency of secondary embryogenesis was obtained from late cotyledonary secondary embryos. Somatic embryos were transferred to MS basal medium and then they germinated within 2 to 4 weeks of culture. Germinated somatic embryos grew normally into plantlets on WPM medium, producing new shoots. The converted plantlets were acclimatized on artificial soil mixture. These results indicate that the repetitive secondary somatic embryogenesis in T amurensis can offer the possibility to use in vitro culture system for the micropropagation.

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References

  1. Agarwal S, Kanwar K, Sharma DR (2004) Factors affecting secondary somatic embryogenesis and embryo maturation in Morus alba L. Scientia Horticulturae 102: 359-368 https://doi.org/10.1016/j.scienta.2004.04.002
  2. Biahoua A, Bonneau L (1999) Control of in vitro somatic embryogenesis of spindle tree (Euonymus europaeus L.) by the sugar type and the osmotic potential of the culture medium. Plant Cell Rep 19: 185-190 https://doi.org/10.1007/s002990050731
  3. Catarina CS, dos Santos Olmedo A, de Andrade Meyer G, Macedo J, de Amorim W, Viana AM (2004) Repetitive somatic embryogenesis of Ocotea catharinensis Mez. (Lauraceae): effect of somatic embryo developmental stage and dehydration. Plant Cell Tiss Org Cult 78: 55-62
  4. Chalupa V (1990) Plant regeneration by somatic embryogenesis from cultured immature embryos of oak (Quercus robur L.) and linden (Tilia cordata MilL). Plant Cell Rep 9: 398-401
  5. Driver JA, Kuniyuki AH (1984) In vitro propagation of paradox walnut rootstock. HortSci 19: 507-509
  6. Gresshoff PM, Doy CH (1972) Development and differentiation of haploid Lycopersicon esculentum (tomato). Planta 107: 161-170 https://doi.org/10.1007/BF00387721
  7. Kim JH, Moon HK, Park JI, Lee BC (1988) Somatic embryogenesis and plant regeneration in callus from hypocotyl segments of Tilia amurensis. In: Genetic Manipulation of Woody Plants. JW Hanover and DE keathley (eds) Plenum Press, New York, pp 473-474
  8. Kim MJ, Hyun JO, Kim SJ, Kim IS (1990) In vitro induction of somatic embryos from primary leaf tissues of Tilia amurensis seedling. Kor J Apiculture 5: 35-42
  9. Karkonen A (2000) Anatomical study of zygotic and somatic embryos of Tilia cordata. Plant Cell, Tiss Org Cult 61: 205-214 https://doi.org/10.1023/A:1006455603528
  10. Lee D K, Suh M H (1989) Propagation of Tilia species by cutting. Kor J Apiculture 4: 56-60
  11. Lloyd E, McCown B (1980) Commercially feasible micropropagation of mountain laurel, Kalmia latirolia, by use of shoot tip culture. Proc Inti Plant Prop Soc 30:421-427
  12. Morsink WAG, Smith VG (1974) Root and shoot development on cuttings of basswood (Tilia americana) as affected by auxin treatments and size cuttings. Can J For Res 4: 246-249 https://doi.org/10.1139/x74-036
  13. Morsink WAG, Smith VG (1975) The effect of some monohydroxybenzoic and dihydroxybenzoic acids as auxin synergists on rooting softwood cuttings of basswood (Tilia americana L.) under mist. Can J For Res 5: 500-502 https://doi.org/10.1139/x75-070
  14. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue. Physiol Plant 15: 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  15. Raemakers CJJM, Jacobsen E, Visser RGF (1995) Secondary somatic ernbryoqenesls and applications in plant breeding. Euphytica 81: 93-107 https://doi.org/10.1007/BF00022463
  16. Sofiari E, Raemakers CJJM, Kanju E, van Lammeren AM, Jacobsen E, Visser RGF (1997) Comparison of NAA and 2,4-D induced somatic embryogenesis in Cassava. Plant Cell Tiss Org Cult 50: 45-56 https://doi.org/10.1023/A:1005844414258
  17. Youn Y, Ohba K (1990) In vitro plantlet regeneration from axillary buds of Tilia amurensis mature trees and clonal variation in tissue culturability. J Kor For Soc 79: 109-114
  18. Youn Y, Ishii K, Saito A, Ohba K (1988) In vitro plantlet regeneration from axillary buds of mature trees of Tilia cordata. J Jpn For Soc 70: 315-317

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