• Journal of Microbiology and Biotechnology
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• v.31 no.9
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• pp.1305-1310
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• 2021

Shikimate is a key high-demand metabolite for synthesizing valuable antiviral drugs, such as the anti-influenza drug, oseltamivir (Tamiflu). Microbial-based strategies for shikimate production have been developed to overcome the unstable and expensive supply of shikimate derived from traditional plant extraction processes. In this study, a microbial cell factory using Corynebacterium glutamicum was designed to overproduce shikimate in a fed-batch culture system. First, the shikimate kinase gene (aroK) responsible for converting shikimate to the next step was disrupted to facilitate the accumulation of shikimate. Several genes encoding the shikimate bypass route, such as dehydroshikimate dehydratase (QsuB), pyruvate kinase (Pyk1), and quinate/shikimate dehydrogenase (QsuD), were disrupted sequentially. An artificial operon containing several shikimate pathway genes, including aroE, aroB, aroF, and aroG were overexpressed to maximize the glucose uptake and intermediate flux. The rationally designed shikimate-overproducing C. glutamicum strain grown in an optimized medium produced approximately 37.3 g/l of shikimate in 7-L fed-batch fermentation. Overall, rational cell factory design and culture process optimization for the microbial-based production of shikimate will play a key role in complementing traditional plant-derived shikimate production processes.

• Food Science of Animal Resources
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• v.41 no.2
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• pp.261-273
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• 2021

Leuconostoc mesenteroides H40 (H40) was isolated from kimchi, and its probiotic properties and neuroprotective effect was evaluated in oxidatively stressed SH-SY5Y cells. H40 was stable in artificial gastric conditions and can be attached in HT-29 cells. In addition, H40 did not produce β-glucuronidase and showed resistant to several antibiotics. The conditioned medium (CM) was made using HT-29 cells refined with heat-killed probiotics (Probiotics-CM) and heated yogurts (Y-CM) to investigate the neuroprotective effect. Treatment with H40-CM not only increased cell viability but also significantly improved brain derived neurotropic factor (BDNF) expression and reduced the Bax/Bcl-2 ratio in oxidatively stress-induced SH-SY5Y cells. Besides, probiotic Y-CM significantly increased BDNF mRNA expression and decreased Bax/Bcl-2 ratio. The physicochemical properties of probiotic yogurt with H40 was not significantly different from the control yogurt. The viable cell counts of lactic acid bacteria in control and probiotic yogurt with H40 was 8.66 Log CFU/mL and 8.96 Log CFU/mL, respectively. Therefore, these results indicate that H40 can be used as prophylactic functional dairy food having neuroprotective effects.

• International journal of advanced smart convergence
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• v.11 no.2
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• pp.22-29
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• 2022

In this paper, for the purpose of designing an real-time unmanned monitoring system, the YOLOv5s (small) object detection model was applied on the NVIDIA TX2^TM AI (Artificial Intelligence) edge computing platform in order to design the fundamental function of an unmanned monitoring system that can detect objects in real time. YOLOv5s was applied to the our real-time unmanned monitoring system based on the performance evaluation of object detection algorithms (for example, R-CNN, SSD, RetinaNet, and YOLOv5). In addition, the performance of the four YOLOv5 models (small, medium, large, and xlarge) was compared and evaluated. Furthermore, based on these results, the YOLOv5s model suitable for the design purpose of this paper was ported to the NVIDIA TX2^TM AI edge computing system and it was confirmed that it operates normally. The real-time unmanned monitoring system designed as a result of the research can be applied to various application fields such as an security or monitoring system. Future research is to apply NMS (Non-Maximum Suppression) modification, model reconstruction, and parallel processing programming techniques using CUDA (Compute Unified Device Architecture) for the improvement of object detection speed and performance.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.5
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• pp.419-424
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• 1992

To study the capacity of callus and shoot formation on seedling stage in soybean, excised hypocotyl, epicotyl, shoot tip, cotyledonary node and primary leaf were cultured on artificial media (MS and B$_{5}$ medium) supplemented with several hormones. Regeneration of shoots was fairly successful from shoot tip and cotyledonary node tissues in soybean. These shoots could be rooted in vitro through tissue culture technique and transplanted normally into soil. Hypocotyl and epicotyl tissues formed only callus, of which growth and appearance were different according to the kinds of media and additives. A small number of shoots were formed from primary leaf tissues, but they did not develop further.r.

• Journal of KIBIM
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• v.13 no.4
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• pp.85-95
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• 2023

In the process of creating drawings based on Building Information Modeling (BIM), automatically generated annotations can cause interference issues depending on the drawing type. This study aims to develop an algorithm for repositioning annotations using genetic algorithms to minimize such interferences. To achieve this, the Application Programming Interface (API) of BIM software was used to analyze data extractable from BIM drawing files. The process involved defining drawing data related to annotation repositioning, preprocessing this data, and deriving optimal placement coordinates for the annotations. Furthermore, applying the developed algorithm to the preliminary design drawings of small and medium-sized neighborhood facilities resulted in approximately a 95.37% decrease in annotation interference, indicating that the proposed algorithm can significantly enhance productivity in BIM-based drawing tasks.

• International Journal of Computer Science & Network Security
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• v.24 no.8
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• pp.21-31
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• 2024

This study investigates the user experience (UX) of first aid training using virtual reality (VR) technology. As VR continues to be adopted for educational and training purposes, it is important to understand how learners perceive and engage with this medium for developing critical skills, such as first aid. In this study, we developed a VR application called "VR First Aid" that includes training modules on three emergency scenarios: heatstroke, shock, and seizure. The application has both tutorial and hands-on training components. We conducted a UX study by administering a questionnaire to participants. The UX of learning through the VR application was then compared to using a traditional e-book format. Results indicate that participants perceived stronger internal behavior control with the e-book but reported better confirmation, engagement, enjoyment, and intention to use when training with the VR system. Gender differences were also explored, revealing that female participants expressed greater interest in learning through the VR platform compared to male participants. These findings provide insights into the strengths and limitations of VR-based first aid training compared to traditional methods. Implications for the design and deployment of VR training systems are discussed, with a focus on optimizing the learner experience and learning outcomes.

• International Journal of Advanced Culture Technology
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• v.12 no.3
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• pp.434-440
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• 2024

SIZE KOREA updates body measurement data every five years, providing essential information for the fashion industry. This anthropometric data is widely used to diagnose consumer body shapes and develop optimal clothing sizes. Artificial intelligence, particularly machine learning, excels in predicting such body shape classifications. This study seeks to enhance the suitability of clothing design by applying the new analytical methodology of machine learning techniques to better capture and classify the unique body shapes of Korean women. In this study, machine learning techniques such as K-means clustering, Silhouette analysis, and Decision Tree analysis were used to classify the lower body shapes of Korean women in their twenties and identify standard body shapes useful for slacks design. The results showed that the lower body of the age group could be classified into three categories: 'small stature' (the majority), 'tall with an average lower body volume,' and 'medium height with a fuller lower body' (the smallest share). The three-cluster approach is validated through Silhouette analysis, which minimizes misclassification. Decision Tree analysis then further defines the criteria for these clusters, highlighting waist height and hip depth as the most significant factors, achieving a classification accuracy of 90.6%. While this study is not directly related to Robotic Process Automation, its detailed analysis of body shapes for slacks patterns can aid RPA in clothing production. Future research should continue integrating machine learning in human body and fashion design studies.

• Korean Journal of Agricultural Science
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• v.15 no.1
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• pp.9-14
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• 1988

This experiment was conducted to study the effect of some environmental factors on mycerial growth, sporulation and sclerotial formation of Botrytis elliptica cultured on artificial media. Mycerial growth of B. elliptica was the most favorable on V-8 juice agar among the seven different media tested and sporulation of the fungus was favorable on the medium under NUV light irradiation. Abundant conidia could be obtained from V-8 juice agar medium by NUV light irradiation after 3 days of incubation at $23^{\circ}C$ under darkness. The optimum temperature for mycerial growth and conidial germination was $23^{\circ}C$ and the mycerial growth was favorable at relatively lower temperature ranged $19^{\circ}C$ to $23^{\circ}C$. The optimum pH of the medium for mycerial growth of this fungus ranged 4.5 to 5.0 and that was inhibited at higher pH of the media. Mycerial growth of the fungus was not highly influenced by irradiation of fluorescent light, however sporulation was stimulated under NUV light irradiation. Sclerotia of B. elliptica were formed when it was cultured at lower temperature below $19^{\circ}C$.

• Journal of Applied Biological Chemistry
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• v.59 no.3
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• pp.221-225
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• 2016

Tolaasin is a pore-forming peptide toxin produced by Pseudomonas tolaasii and causes a brown blotch disease by disrupting membrane structures of cultivated mushrooms. The mechanism and characteristics of tolaasin pore formation are not known in detail; however, tolaasin pores have been demonstrated in the artificial lipid bilayer. Since the tolaasin pore appeared less frequently and unstable in lipid bilayer, a mismatch between the length of tolaasin pore and the thickness of lipid membrane had been suggested. Therefore, tolaasin-induced hemolyses were measured by the additions of phospholipids composed of various fatty acids with different carbon numbers. When phosphatidylethanolamines made with two decanoic acids (C10:0, 1,2-didecanoyl-sn-glycero-3-phosphoethanolamine; DDPE), myristic acids (C14:0, 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine), and stearic acids (C18:0, 1,2-distearoyl-sn-glycero-3-phosphoethanolamine) were added to the buffer containing RBCs and tolaasin peptides, DDPE facilitated the tolaasin-induced hemolysis while the other two phospholipids showed no effects. At various concentrations of DDPE, the tolaasin-induced hemolysis was stimulated as a dose-dependent manner. The phospholipids composed of mediumchain fatty acids stabilize the tolaasin pore probably by binding between the pore structure and membrane phospholipids and making the membrane thickness thinner around the pore. These results showed that tolaasin molecules make more stable pores in the membrane made with phospholipids composed of medium length fatty acids, suggesting that the length of tolaasin pore is a little shorter than the thickness of RBC membrane.

• Journal of Plant Biotechnology
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• v.36 no.1
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• pp.18-22
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• 2009

This work was conducted to establish an efficient plant regeneration system for genetic transformation and the in vitro conservation of Sedum sarmentosum genetic resources. Effects of glutamine and $AgNO_3$ on plant regeneration between two genotypes were investigated using MS media supplemented with 0.2 mg/L NM and 3.0 mg/L BA. Calluses were formed on leaf explants placed on MS solid media supplemented with 3 mg/L 2,4-D and 1 mg/L BA. Calluses of Keumsan local strain produced shoots at a frequency of up to 100% after 50 days of culture on medium supplemented with glutamine. The highest number of shoots per callus was 17.6 at 350 mg/L glutamine. However, calluses of Wanju local strain gave rise to no shoots under the same culture conditions. Likewise, calluses of Keumsan local strain produced shoots at a frequency of up to 100% after 50 days of culture on medium supplemented with $AgNO_3$ whereas Wanju local strain sporadically produced shoots. The highest number of shoots per callus of Keumsan local stain was 16.1 at $15{\mu}M$ $AgNO_3$. Regenerated shoots were subcultured on hormone-free MS medium for rooting and shoot growth, and then 3-5 cm high plantlets were transplanted to the artificial soils comprising vermiculite and perlite, where they survived at a frequency of 88-100%. After being transplanted into upland soil:sand (1:1, v/v) in a greenhouse, regenerated plants showed a morphologically normal growth.