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http://dx.doi.org/10.5851/kosfa.2020.e97

Physicochemical Analysis of Yogurt Produced by Leuconostoc mesenteroides H40 and Its Effects on Oxidative Stress in Neuronal Cells  

Lee, Na-Kyoung (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
Lim, Sung-Min (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
Cheon, Min-Jeong (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
Paik, Hyun-Dong (Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
Publication Information
Food Science of Animal Resources / v.41, no.2, 2021 , pp. 261-273 More about this Journal
Abstract
Leuconostoc mesenteroides H40 (H40) was isolated from kimchi, and its probiotic properties and neuroprotective effect was evaluated in oxidatively stressed SH-SY5Y cells. H40 was stable in artificial gastric conditions and can be attached in HT-29 cells. In addition, H40 did not produce β-glucuronidase and showed resistant to several antibiotics. The conditioned medium (CM) was made using HT-29 cells refined with heat-killed probiotics (Probiotics-CM) and heated yogurts (Y-CM) to investigate the neuroprotective effect. Treatment with H40-CM not only increased cell viability but also significantly improved brain derived neurotropic factor (BDNF) expression and reduced the Bax/Bcl-2 ratio in oxidatively stress-induced SH-SY5Y cells. Besides, probiotic Y-CM significantly increased BDNF mRNA expression and decreased Bax/Bcl-2 ratio. The physicochemical properties of probiotic yogurt with H40 was not significantly different from the control yogurt. The viable cell counts of lactic acid bacteria in control and probiotic yogurt with H40 was 8.66 Log CFU/mL and 8.96 Log CFU/mL, respectively. Therefore, these results indicate that H40 can be used as prophylactic functional dairy food having neuroprotective effects.
Keywords
probiotics; Leuconostoc mesenteroides; neuroprotective effect; probiotic yogurt; oxidative stress;
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1 Yu HS, Lee NK, Choi AJ, Choe JS, Bae CH, Paik HD. 2019c. Anti-inflammatory potential of probiotic strain Weissella cibaria JW15 isolated from kimchi through regulation of NF-κB and MAPKs pathways in LPS-induced RAW 264.7 cells. J Microbiol Biotechnol 29:1022-1032.   DOI
2 Zhao LL, Wang XL, Tian Q, Mao XY. 2016. Effect of casein to whey protein ratios on the protein interactions and coagulation properties of low-fat yogurt. J Dairy Sci 99:7768-7775.   DOI
3 Lee NK, Kim SY, Han KJ, Eom SJ, Paik HD. 2014. Probiotic potential of Lactobacillus strains with anti-allergic effects from kimchi for yogurt starters. LWT-Food Sci Technol 58:130-134.   DOI
4 Lee NK, Han KJ, Son SH, Eom SJ, Lee SK, Paik HD. 2015. Multifunctional effect of probiotic Lactococcus lactis KC24 isolated from kimchi. LWT-Food Sci Technol 64:1036-1041.   DOI
5 Lee SY, Hur SJ. 2019. Mechanisms of neuroprotective effects of peptides derived from natural materials and their production and assessment. Compr Rev Food Sci Food Saf 18:923-935.   DOI
6 Liu L, Cavanaugh JE, Wang Y, Sakagami H, Mao Z, Xia Z. 2003. ERK5 activation of MEF2-mediated gene expression plays a critical role in BDNF-promoted survival of developing but not mature cortical neurons. Proc Natl Acad Sci USA 100:8532-8537.   DOI
7 Lobo V, Patil A, Phatak A, Chandra N. 2010. Free radicals, antioxidants and functional foods: Impact on human health. Pharmacogn Rev 4:118-126.   DOI
8 Martin CR, Osadchiy V, Kalani A, Mayer EA. 2018. The brain-gut-microbiome axis. CMGH Cell Mol Gastroenterol Hepatol 6:133-148.   DOI
9 Numakawa T, Suzuki S, Kumamaru E, Adachi N, Richards M, Kunugi H. 2010. BDNF function and intracellular signaling in neurons. Histol Histopathol 25:237-258.
10 O'Toole PW, Marchesi JR, Hill C. 2017. Next-generation probiotics: The spectrum from probiotics to live biotherapeutics. Nat Microbiol 2:17057.   DOI
11 Pamplona R, Barja G. 2006. Mitochondrial oxidative stress, aging and caloric restriction: The protein and methionine connection. Biochim Biophys Acta 1757:496-508.   DOI
12 Park J, Lee J, Yeom Z, Heo D, Lim YH. 2017. Neuroprotective effect of Ruminococcus albus on oxidatively stressed SHSY5Y cells and animals. Sci Rep 7:14520.   DOI
13 Pardillo-Diaz R, Carrascal L, Munoz MF, Ayala A, Nunez-Abades P. 2016. Time and dose dependent effects of oxidative stress induced by cumene hydroperoxide in neuronal excitability of rat motor cortex neurons. Neurotoxicology 53:201-214.   DOI
14 Quigley EM. 2017. Microbiota-brain-gut axis and neurodegenerative diseases. Curr Neurol Neurosci Rep 17:94.   DOI
15 Ranuh R, Athiyyah AF, Darma A, Risky VP, Riawan W, Surono IS, Sudarmo SM. 2019. Effect of the probiotic Lactobacillus plantarum IS-10506 on BDNF and 5HT stimulation: Role of intestinal microbiota on the gut-brain axis. Iran J Microbiol 11:145-150.
16 Shao X, Fang K, Medina D, Wan J, Lee JL, Hong SH. 2019. The probiotic, Leuconostoc mesenteroides, inhibits Listeria monocytogenes biofilm formation. J Food Saf 2019:e12750.
17 Son SH, Jeon H, Yang SJ, Sim MH, Kim YJ, Lee NK, Paik HD. 2018. Probiotic lactic acid bacteria isolated from traditional Korean fermented foods based on β-glucosidase activity. Food Sci Biotechnol 27:123-129.   DOI
18 Sun J, Ling Z, Wang F, Chen W, Li H, Jin J, Zhang H, Pang M, Yu J, Liu J. 2016. Clostridium butyricum pretreatment attenuates cerebral ischemia/reperfusion injury in mice via anti-oxidation and anti-apoptosis. Neurosci Lett 613:30-35.   DOI
19 Elahi MM, Kong YX, Matata BM. 2009. Oxidative stress as a mediator of cardiovascular disease. Oxid Med Cell Longev 2:920580.
20 Egan MF, Kojima M, Callicott JH, Goldberg TE, Kolachana BS, Bertolino A, Zaitsev E, Gold B, Goldman D, Dean M, Lu B, Weinberger D. 2003. The BDNF val66met polymorphism affects activity-dependent secretion of BDNF and human memory and hippocampal function. Cell 112:257-269.   DOI
21 Ghaisas S, Maher J, Kanthasamy A. 2016. Gut microbiome in health and disease: Linking the microbiome-gut-brain axis and environmental factors in the pathogenesis of systemic and neurodegenerative diseases. Pharmacol Ther 158:52-62.   DOI
22 Herrera A, Pineda J, Antonio MT. 2013. Toxic effects of perinatal arsenic exposure on the brain of developing rats and the beneficial role of natural antioxidants. Environ Toxicol Pharmacol 36:73-79.   DOI
23 Ishimwe N, Daliri EB, Lee BH, Fang F, Du G. 2015. The perspective on cholesterol‐lowering mechanisms of probiotics. Mol Nutr Food Res 59:94-105.   DOI
24 Jang HJ, Lee NK, Paik HD. 2019. Probiotic characterization of Lactobacillus brevis KU15153 showing antimicrobial and antioxidant effect isolated from kimchi. Food Sci Biotechnol 28:1521-1528.   DOI
25 Jang HJ, Song MW, Lee NK, Paik HD. 2018. Antioxidant effects of live and heat-killed probiotic Lactobacillus plantarum Ln1 isolated from kimchi. J Food Sci Technol 55:3174-3180.   DOI
26 Kennedy PJ, Murphy AB, Cryan JF, Ross PR, Dinan TG, Stanton C. 2016. Microbiome in brain function and mental health. Trends Food Sci Technol 57:289-301.   DOI
27 Klippel BF, Duemke LB, Leal MA, Friques AGF, Dantas EM, Dalvi RF, Gava AL, Pereira TMC, Andrade TU, Meyrelles SS, Campagnaro BP, Vasquez EC. 2016. Effects of kefir on the cardiac autonomic tones and baroreflez sensitivity in spontaneously hypertensive rats. Front Physiol 7:211.
28 Wang H, Lee IS, Braun C, Enck P. 2016. Effect of probiotics on central nervous system functions in animals and humans: A systematic review. J Neurogastroenterol Motil 22:589-605.   DOI
29 Dussert S, Davey MW, Laffargue A, Doulbeau S, Swennen R, Etienne H. 2006. Oxidative stress, phospholipid loss and lipid hydrolysis during drying and storage of intermediate seeds. Physiol Plant 127:192-204.   DOI
30 Ton AMM, Campagnaro BP, Alves GA, Aires R, Côco LZ, Arpini CM, Oliveira TG, Campos-Toimil M, Meyrelles SS, Pereira TMC, Vasquez EC. 2020. Oxidative stress and dementia in Alzheimer's patients: Effects of synbiotic supplementation. Oxid Med Cell Longev 2020:2638703.
31 Wang X, Michaelis EK. 2010. Selective neuronal vulnerability to oxidative stress in the brain. Front Aging Neurosci 2:12.
32 Chambers L, Avery A, Dalrymple J, Farrell L, Gibson G, Harrington J, Rikjers G, Rowland I, Spiro A, Varela-Moreiras G, Vokes L, Younge L, Whelan K, Stanner S. 2019. Translating probiotic science into practice. Nutr Bull 44:165-173.   DOI
33 AOAC International. 1999. Caseins and casein micelle structure. 16th ed. AOAC International, Washington, DC, USA.
34 Azmi NH, Ismail N, Imam MU, Ismail M. 2013. Ethyl acetate extract of germinated brown rice attenuates hydrogen peroxide-induced oxidative stress in human SH-SY5Y neuroblastoma cells: Role of anti-apoptotic, pro-survival and antioxidant genes. BMC Complemt Altern Med 13:177.   DOI
35 Campedelli I, Mathur H, Salvetti E, Clarke S, Rea MC, Torriani S, Ross RP, Hill C, O'Toole PW. 2019. Genus-wide assessment of antibiotic resistance in Lactobacillus spp. Appl Environ Microbiol 85:e01738-18.
36 Cheon MJ, Lim SM, Lee NK, Paik HD. 2020. Probiotic properties and neuroprotective effects of Lactobacillus buchneri KU200793 isolated from Korean fermented foods. Int J Mol Sci 21:1227.   DOI
37 Clinical and Laboratory Standards Institute. 2012. Performance standards for antimicrobial disk susceptibility testing; approved standard. 11th ed. CLSI Document M02-A11. Clinical and Laboratory Standards Institute, Wayne, PA, USA.
38 Dabek M, McCrae SI, Stevens VJ, Duncan SH, Louis P. 2008. Distribution of β-glucosidase and β-glucuronidase activity and of β-glucuronidase gene gus in human colonic bacteria. FEMS Microbiol Ecol 66:487-495.   DOI
39 Yang SJ, Lee JE, Lim SM, Kim YJ, Lee NK, Paik HD. 2019. Antioxidant and immune-enhancing effects of probiotic Lactobacillus plantarum 200655 isolated from kimchi. Food Sci Biotechnol 28:491-499.   DOI
40 Wang Y, Kasper LH. 2014. The role of microbiome in central nervous system disorders. Brain Behav Immun 38:1-12.   DOI
41 Yi YJ, Lim JM, Lim JM, Gu S, Lee WK, Oh E, Lee SM, Oh BT. 2017. Potential use of lactic acid bacteria Leuconostoc mesenteroides as a probiotic for the removal of Pb (II) toxicity. J Microbiol 55:296-303.   DOI
42 Yoon JY, Kim D, Kim EB, Lee SK, Lee M, Jang A. 2018. Quality and lactic acid bacteria diversity of pork salami containing kimchi powder. Korean J Food Sci Anim Resour 38:912-926.   DOI
43 Yu HS, Jang HJ, Lee NK, Paik HD. 2019a. Evaluation of the probiotic characteristics and prophylactic potential of Weissella cibaria strains isolated from kimchi. LWT-Food Sci Technol 112:108229.   DOI
44 Yu HS, Lee NK, Choi AJ, Choe JS, Bae CH, Paik HD. 2019b. Antagonistic and antioxidant effect of probiotic Weissella cibaria JW15. Food Sci Biotechnol 28:851-855.   DOI