Effects of Some Environmental Factors on Mycerial Growth, Sporulation and Sclerotial Formation of Botrytis elliptica (Berk.) Cooke

백합(百合) 잎마름병균(病菌)의 균사생장(菌絲生長), 포자형성(胞子形成) 및 균핵형성(菌核形成)에 미치는 몇가지 환경요인(環境要因)의 영향(影響)

This experiment was conducted to study the effect of some environmental factors on mycerial growth, sporulation and sclerotial formation of Botrytis elliptica cultured on artificial media. Mycerial growth of B. elliptica was the most favorable on V-8 juice agar among the seven different media tested and sporulation of the fungus was favorable on the medium under NUV light irradiation. Abundant conidia could be obtained from V-8 juice agar medium by NUV light irradiation after 3 days of incubation at $23^{\circ}C$ under darkness. The optimum temperature for mycerial growth and conidial germination was $23^{\circ}C$ and the mycerial growth was favorable at relatively lower temperature ranged $19^{\circ}C$ to $23^{\circ}C$. The optimum pH of the medium for mycerial growth of this fungus ranged 4.5 to 5.0 and that was inhibited at higher pH of the media. Mycerial growth of the fungus was not highly influenced by irradiation of fluorescent light, however sporulation was stimulated under NUV light irradiation. Sclerotia of B. elliptica were formed when it was cultured at lower temperature below $19^{\circ}C$.

백합(百合) 잎마름병균(病菌)(Botrytis elliptica)의 균사생장(菌絲生長), 포자형성(胞子形成) 및 균핵형성(菌核形成)에 미치는 주요(主要)한 환경요인(環境要因)의 영향(影響)을 밝히고자 실험(實驗)을 실시(實施)한 결과는 다음과 같다. 1. B. elliptica는 V-8 juice agar 배지(培地)에서 균사생장(菌絲生長)이 가장 양호(良好)하였으며 NUV light 조사시(照射時) 다른 배지(培地)에 비해 분생포자(分生胞子)의 형성(形成)도 빨랐고 형성량(形成量)도 많았으며 3일간(日間) $23^{\circ}C$에 배양(培養)한 후 NUV light를 조사(照射)하면 보다 다량(多量)의 분생포자(分生胞子)를 얻을 수 있었다. 2. 균(菌)의 생장(生長) 및 포자발아적온(胞子發芽適溫)은 $23^{\circ}C$였으며 고온(高溫)보다는 $19{\sim}23^{\circ}C$의 비교적(比較的) 저온(低溫)에서 균(菌)의 생장(生長)이 빨랐다. 3. pH 4.5~5.0에서 균(菌)의 생육(生育)이 좋았으나 pH가 높아질수록 생장(生長)이 억제(抑制)되었다. 4. 광(光)은 균사(菌絲)의 생육(生育)에는 큰 영향이 없었으나 포자(胞子)의 형성(形成)에는 광(光)의 종류(種類)가 매우 크게 작용(作用)하여 NUV light 조사(照射)에 의(依)해서만 분생포자(分生胞子)가 형성(形成)되었다. 균핵(菌核)은 $19^{\circ}C$이하(以下)의 배양(培養)에서만 형성(形成)되었다.