• The Plant Pathology Journal
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• 제26권4호
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• pp.313-320
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• 2010

Genetic instability of the rice blast fungus Magnaporthe oryzae has been suggested as a major factor underlying the rapid breakdown of host resistance in the field. However, little information is available on the mechanism of genetic instability. In this study, we assessed the stability of repetitive DNA elements and several key phenotypic traits important for pathogenesis after serially transferring two isolates though rice plants and an artificial medium. Using isolate 70-15, we obtained a total of 176 single-spore isolates from 10 successive rounds of culturing on artificial medium. Another 20 isolates were obtained from germ tubes formed at the basal and apical cells of 10 three-celled conidia. Additionally, 60 isolates were obtained from isolate KJ201 after serial transfers through rice plants and an artificial medium. No apparent differences in phenotypes, including mycelial growth, conidial morphologies, conidiation, conidial germination, appressorium formation, and virulence, or in DNA fingerprints using MGR586, MAGGY, Pot2, LINE, MG-SINE and PWL2 as probes were observed among isolates from the same parent isolate. Southern hybridization and sequence analysis of two avirulence genes, AVR-Pita1 and AVR-Pikm, showed that both genes were also maintained stably during 10 successive generations on medium and plants. However, one reversible loss of restriction fragments was found in the telomere-linked helicase gene (TLH1) family, suggesting some telomere regions may be more unstable than the rest of the genome. Taken together, our results suggest that phenotype and genotype of M. oryzae isolates do not noticeably change, at least up to 10 successive generations on a cultural medium and in host plants.

• 한국중재학회지:중재연구
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• 제30권3호
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• pp.95-113
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• 2020

The worldwide diffusion of COVID-19 contributes to electronic commerce all over the world. The proliferation of high volume and small value electronic commerce naturally has combined artificial intelligence with online dispute resolution (ODR). This paper investigates the age effect on Artificial Intelligence acceptability in online dispute resolution and its empirical findings are as follows. First, seven measures out of the nine employed in this case study shows a coherent dynamic pattern over the age spectrum. In other words, the total samples are a heterogenous group rather than a homogeneous one. Second, medium answer occupies a non-negligible portion across answers from nine research questions. It seems to indicate that a considerable portion of Korean respondents are hesitant to make a choice on artificial intelligence at this juncture. Third, all of the respondents agree that the introduction of AI to the dispute resolution could contribute to the hastening of the dispute resolution process. Fourth, most of the respondents agree that artificial intelligence might have the cognitive ability but not the sympathetic or affective ability to handle the electronic commerce disputes.

• 정보화정책
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• 제30권1호
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• pp.67-88
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• 2023

제조물책임 범위 확장이 필요한 것은 제조물책임법 제정 시 산업 환경이 변했기 때문이다. 사람이 코딩한 알고리즘과 다르게, 인공지능은 기계학습에 따라 블랙박스화 되면서 개발자도 결과를 설명하지 못한다. 특히, 인공지능으로 인하여 발생하는 문제의 원인을 알 수 없기 때문에 책임소재도 불분명할뿐더러 피해자 배상도 쉽지 않다. 동산 등으로 한정된 제조물책임법에 따라 소프트웨어(SW)나 인공지능은 무체물로 제조물성이 인정되지 않기 때문이다. 고육지책으로 매체에 저장되거나 내장된 경우에는 제조물성이 인정될 수 있다고 한다. 그러나 매체에 따라 달리 적용되는 것은 타당하지 않다. EU는 인공지능이 포함된 경우, 제조물책임을 인정하는 제조물책임지침 개정을 추진 중이다. 피해자에 대한 보상이 제조물책임법이 추구하는 가치임에도 제조물성에 치중하여 본질을 간과해왔다. 다만, 인공지능이 채택된 서비스를 이용하여 발생한 사고라도 무조건적으로 제조물책임을 지우는 것이 아닌 실질적인 위험성에 따른 기준이 제시되는 것이 바람직하다.

• 한국균학회지
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• 제29권1호
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• pp.52-60
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• 2001

This study was carried out to obtain the basic data on artificial culture of Hohenbuehelia petaloides. The optimum medium are glucose peptone medium (GP), Hennerberg medium, Phellinus igniarius medium (PIM), Lentinus edodes medium (LEM), Czapek dox medium. The optimum condition for the mycelial growth was $30^{\circ}C$ and pH 6.0. The carbon sources such as dextrine, fructose and lactose were favorable to mycelial growth. The optimal concentrations of carbon sources are 10% dextrin and fructose. As nitrogen sources, tryptone, casamino acid and histidine appeared to be favorable. The optimal concentrations of nitrogen sources are 1% soy tone and 0.3% ammonium nitrate. The optimal concentration of yeast extract is 0.4%. The mineral nutrients of $KH_2PO_4$, $K_2HPO_4\;and\;MgSO_4{\cdot}7H_2O$ were effective and the optimal concentrations were 0.046, 0.1 and 0.05%, respectively.

• 한국지하수토양환경학회지:지하수토양환경
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• 제20권4호
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• pp.22-30
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• 2015

For the preliminary investigations of the bioclogging on groundwater artificial recharge system, studies for conditions and analytical methods of biofilm formation on sediments were performed. Based on the tested results, following conditions were determined for biofilm formation on batch process: optimum period for biofilm formation (30 days), the proper inoculating water (pond water), medium (minimum salt medium with 0.1% yeast extract). Procedures for the measurement of ATP and DHA were also determined. Biomass extract was used for ATP measurement, while sediment itself for DHA. Effects of metals on the biofilm formation were investigated under the determined conditions. Different sensitivities and orders were found depending on tested metals and measurement methods. In general, biomass measurement by ATP and viable cell count showed higher sensitivity than that of DHA. Following toxicity orders were also appeared for ATP and viable cell: Cu ≈ Cd > As(III).

• Journal of Microbiology and Biotechnology
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• 제22권7호
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• pp.939-946
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• 2012

Antibiotic production with Streptomyces sindenensis MTCC 8122 was optimized under submerged fermentation conditions by artificial neural network (ANN) coupled with genetic algorithm (GA) and Nelder-Mead downhill simplex (NMDS). Feed forward back-propagation ANN was trained to establish the mathematical relationship among the medium components and length of incubation period for achieving maximum antibiotic yield. The optimization strategy involved growing the culture with varying concentrations of various medium components for different incubation periods. Under non-optimized condition, antibiotic production was found to be $95{\mu}g/ml$, which nearly doubled ($176{\mu}g/ml$) with the ANN-GA optimization. ANN-NMDS optimization was found to be more efficacious, and maximum antibiotic production ($197{\mu}g/ml$) was obtained by cultivating the cells with (g/l) fructose 2.7602, $MgSO_4$ 1.2369, $(NH_4)_2PO_4$ 0.2742, DL-threonine 3.069%, and soyabean meal 1.952%, for 9.8531 days of incubation, which was roughly 12% higher than the yield obtained by ANN coupled with GA under the same conditions.

• 한국잠사곤충학회지
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• 11호
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• pp.55-57
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• 1970

1. 교잡종의 냉장침산잠종 재냉장 적기는 침산 후 48∼72 시간 즉 2∼3일이다. 2. 원종의 냉장침산잠종 재냉장적기는 침산후 72시간 즉 3일이다. 3, 교잡종이나 원종을 막논하고 냉장침산잠종의 재냉장적기는 일언으르써 침산후 72시이라고 판단되며 입고방법은 중간온도 보호의 영향력이 있다고 판단된다.

• 융합정보논문지
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• 제9권3호
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• pp.16-21
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• 2019

우리나라 중소기업은 현재 국내 외 다양한 환경 요인(경쟁력 확보 및 우수 제품 개발 등)으로 인하여 산업 구조가 과거에 비해 빠르게 변화하고 있는 상황이다. 특히, 인공지능과 관련된 다양한 장비가 제조현장에 투입되면서 스마트팩토리 환경에서 생산되는 데이터 수집 및 활용의 중요성이 점점 증가하고 있다. 본 논문에서는 최근 중소기업 제조 현장이 스마트팩토리화 되면서 제조 현장에서 생산되는 제품의 프로세스를 향상시키기 위한 인공지능 기반 스마트팩토리 모델을 제안한다. 제안 모델은 갈수록 치열해지는 제조 환경의 경쟁력 확보 및 생산 비용 절감을 최소화시키는 것이 목적이다. 제안 모델은 인공지능 기반의 스마트팩토리 현장에서 생산되는 제품의 정보뿐만 아니라 제품 생산에 소비되는 노동력, 노동 근무 시간 및 가동 공장기계 상태 등을 모두 고려하여 관리한다. 또한, 제안 모델에서 생산되는 데이터는 유사 기업과 시스템 연계 및 정보 공유가 가능하기 때문에 제조 현장 운영의 기업간 전략적 협력이 가능하다.

• 한국가축번식학회지
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• 제12권1호
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• pp.42-47
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• 1988

This experiment was carried out to investigate the sex ratio of produced lamb after artificial insemination into the cervix with spermatozoa from the top and bottom portion of ram semen separated by diluting semen a column of protein, and after implantation into the uterus with normal morulae and blastocyst cultured in BMOG-3 and Ham F-10 medium containing H-Y anbibody and complement treated with spleen and testis, respectively. All embryos developed to morulae and blastocyst were cultrued in medium under gas phase of 5% CO2 in air at 37$^{\circ}C$, 24 hrs. Estrus of ewes induced by a MAP vaginal sponge and 750 IU PMSG during the non-breeding season. The result obtained in these experiments were summarized as follows: 1. The ratio of heating, lambing, and prolificacy after artificial inseminatin into the cervix of ewes induced by a MAP vaginal sponge and 750 IU PMSG during the non-breeding season were 100%, 40% and 2.74 heads, respectively. 2. Involving 44 ewes, spermatozoa from the top of the protein column produced 23.1% male and 76.9% female lambs, while spermatozoa from the bottom of the column produced 81.3% male and 18.8% female offspring, respectively. 3. The sex ratio (male-to-female) of progeny produced after implantation with normal morulae and blastocyst cultured in medium containing H-Y antibody and complement treated with spleen and testis were 21.0%, 79.0% and 17.4%, 82.6%, respectively.

• 한국균학회지
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• 제35권2호
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• pp.76-80
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• 2007

잎새버섯의 우량계통을 선발하여 농가에 보급하기 위하여 수집균주들의 균사체 생육 최적조건을 검토한 결과는 다음과 같다. 잎새버섯 수집균주의 생장에 적합한 배지는 PDA배지가 선발되었으며, 균사배양 적온은 $25^{\circ}C$로 나타났다. 잎새버섯의 균사생장에 적합한 수소이온 농도는 pH $4{\sim}5$로 약산성에서 균사생장이 우수하였다. 균사생장에 적합한 탄소원으로는 glucose와 fructose, 질소원으로는 peptone이 우수하였으며 C/N율은 $10{\sim}20$으로 질소 요구도가 다소 높은 것으로 나타났다.