• Biotechnology and Bioprocess Engineering:BBE
• /
• v.8 no.3
• /
• pp.183-186
• /
• 2003

The artificial gene coding for anticoagulant hirudin was placed under the control of the GAL 10 promoter and expressed in the galactokinase-deficient strain (Δgal1) of Saccharomyces cerevisiae, which uses galactose only as a gratuitous inducer in order to avoid its consumption. For efficient production of recombinant hirudin, a carbon source other than galactose should be provided in the medium to support growth of the Δgal1 strain. Here we demonstrate the successful use of glucose in the fed-batch fermentation of the Δgal1 strain to achieve efficient production of recombinant hirudin, with a yield of up to 400 mg hirudin/L.

• The Korean Journal of Mycology
• /
• v.44 no.3
• /
• pp.166-170
• /
• 2016

Ectomycorrhizal fungi are associated with plants roots and acquire significant amounts of nitrogen sources from the soil. For artificial cultivation, mass production of ectomycorrhizal fungi in liquid media is required. We studied the edible ectomycorrhizal mushrooms Hygrophorus russula, Ramaria fumigata, Sarcodon aspratus, and Tricholoma matsutake. All strains except S. aspratus NIFoS 2031 grew generally well on modified Melin-Norkran's (MMN) medium compared to on other media. All strains analyzed in this study showed significantly higher growth on organic nitrogen. Specifically, two strains of H. russula significantly responded to both tryptone and neopeptone media. Among different species and strains, there were clear differences in the capacity to grow on animal-based organic nitrogen sources.

• Journal of the Korea Safety Management & Science
• /
• v.19 no.1
• /
• pp.227-236
• /
• 2017

Smart Manufacturing Factory is a paradigm of the future lead to the fourth industrial revolution that led Germany and the United States. Now the automation of the production facility and won a certain degree, and through the process of integrating the entire process, including planning, design, distribution of information and communication technology products in emerging as a core competitiveness of the national economy. In particular, the company accelerated the smart factory building in order to improve the manufacturing industry, cost savings and productivity simply to incorporate internet of things(IoT),Robot, artificial intelligence, big data technology as a factory automation level of sophistication of the system and out to progress to the level that replaces human labor have. In this we should look at the trend of promoting domestic and foreign factories want to present these smart strategies for Korea.

• Proceedings of the KSR Conference
• /
• 2006.11b
• /
• pp.1391-1404
• /
• 2006

Recently, medium or slight earthquakes was occurred in the Korea Peninsula and seismic design is considered seriously in the railroad facilities as case of other civil engineering facilities. In this study, it selected the Seongnam-Yeoju railroad 6th section and seismic analysis was accomplished. Specially, unlike existing seismic analysis using the artificial earthquake and the real earthquake, seismic analysis using a seismic vibration and the train vibration was accomplished. 1-D and 2-D ground response analysis of the railroad bed and 3-D Finite element analysis in the bridge connection section of high landfill slope was accomplished. Also, slope stability analysis and the evaluation of liquefaction was accomplished.

• Journal of Intelligence and Information Systems
• /
• v.1 no.1
• /
• pp.123-134
• /
• 1995

인공신경망을 이용한 기업도예측에 관한 연구는 일반적으로 대기업을 대상으로 수행되고 있으며, 분석자료로는주로 재무제표에서 얻어지는 재무정보를 사용하고 있다. 이들 대기업의 재무정보들은 비교적양이 풍부하고 신뢰성이 높기 때문에 인공신경망을 이용한 도산예측의 적중률이 80%∼85%의 높은 수준을 보이고 있다. 하지만, 중소기업이 재무정보는 불충분할 뿐만 아니라 신뢰성이 낮을 가능성이 높기 때문에, 중소기업의 도산예측에 있어서 재무정보만을 사용하게 되면 그 정확도가 떨어지게 된다. 본 연구에서는 인공신경망을 이용한 중소기업의 도산예측에 있어서, 재무정보를 보완할 수 있는 비재무정보의 유용성을 검증하였다. 연구결과 본 연구에서 사용한 비재무정보가 획득가능한 비재무정보중 극히 일부에 지나지 않았음에도 불고하고, 재무정보만을 사용하였을 때보다 예측력이 10%정도나 향상되었다.

• Plant Disease and Agriculture
• /
• v.1 no.2
• /
• pp.18-21
• /
• 1995

Mycosphaerella nawae, the causal organism, of spotted leaf casing disease of persimmon, was isolated from infected leaves showing typical symptom. The cultural characteristics of the fungus were compared on artificial media. Among 24 different combinations of culture media and supplements, oatmeal agar+persimmon leaf extract (PLE) and PAD+ PLE+streptomycin showed the highest rate of isolating as 57.1%. The best medium for mycelial growth of the fungus was PDA+persimmon leaf powder (PLP). The colony diameter was reached 47mm for 30 days at 2$0^{\circ}C$. PDA+PLE also supported good mcyelial growth showing 46mm of diameter in same condition. The optimum growth temperature of this fungus in PDA was recognized fairly low. The mycelial growth was higher at 2$0^{\circ}C$ than 15$^{\circ}C$. The variation of pH between pH 4 to pH 8 did not affect to the mycelial growth of the pathogen.

• Journal of Korea Multimedia Society
• /
• v.25 no.10
• /
• pp.1433-1447
• /
• 2022

Recently, as the demand for physical security technology to prevent leakage of technical and business information of companies and public institutions increases, the high tech companies are operating X-ray security checkpoints at building entrances to protect their intellectual property and technology. X-ray security checkpoints are operated to detect cameras and storage media that may store or leak important technologies in the bags of people entering and leaving the building. In this study, we propose an X-ray security checkpoint system that automatically detects a storage medium in an X-ray image using a deep learning based object detection method. The proposed system consists of an edge computing unit and a cloud-computing unit. We employ the RetinaNet for automatic storage media detection in the X-ray security checkpoint images. The proposed approach achieved mAP of 95.92% on private dataset.

• International Journal of Advanced Culture Technology
• /
• v.12 no.1
• /
• pp.123-127
• /
• 2024

In the early 20th century, Walter Benjamin analyzed the changes in the value of traditional art forms under the industrial era and the changes in the aesthetic attitude of the masses. A century later, in the contemporary multi-art world, the traditional medium of shelf painting is once again experiencing a similar situation as the last century. Emerging technology display modes such as digital virtual reality and digital immersive experience can achieve digital reproduction of paintings on shelves and reach a certain level of performance, which once again shocks the public's aesthetic perception. This paper attempts to illustrate the outstanding characteristics of the new art form after digital reconstruction by exploring the transformation and sublimation of digital technology to shelf painting. We predict that art research on future reality and augmented reality according to the artificial intelligence era will be conducted in depth in the future.

• Journal of Biomedical Engineering Research
• /
• v.16 no.4
• /
• pp.463-470
• /
• 1995

Complete prelining of artificial vascular grafts with autologous endothelial cells may be one of the ideal solutions to obtain a nonthrombogenlc blood-contacting surface. To establish an intact endothelial cell monolayer on a prosthetic surface at the time of implantation,a sufficient number of endothelial cells and adequate propagation condition In cell culture are prerequisites. In this experimental study, endothelial cells from microvessels of adult human oriental adipose tissue were enzymatically harvested, and optimal culture conditions for proliferation of the endothelial cells in cell culture were examined. Human oriental adipose tissue was digested with collagenase and endothelial cells were separated from other stromal elements by mesh filtration method. Cultured cells were identified as endothelial cells by immunofluorescent staining for factor VIII-related antigen. Proliferation in usual 20% fetal bovine serum (FBS) medium or medium containing endothelial cell growth factor (ECGF)(5 ng/ml) and heparin (HEP)(1,000 units/ml) were compared,and the effects of adding compounds that increase intracellular cyclic adenosine monophosphate levels, that is,cholera toxin (CT)(1 $\mu\textrm{g}$/ml) and isobutylmethylxanthine (IBMX)(0.2 ml),were also analyzed. In total,following eight media groups were examined. 1) FBS medium + ECGF + HEP, 2) FBS medium + ECGF + HEP+CT, 3) FBS medium+ECGF+HEP+lBMX, 4) FBS medium+ECGF+HEP+CT+ IBMX, 5) FBSmedium, 6) FBS medium +CT, 7) FBS medium + IBMX, 8) FBS medium + CT + IBMX. It was shown that the medium containing ECGF + HEP with or without cholera toxin was most efficient in Stimulating cell proliferation. IBMX was considered to have antagonistic effect to ECGF. Among experimental groups without ECGF and HEP, the addition of cholera toxin and IBMX was shown to significantly potentiate cell proliferation. This results could provide a practical method for use of cultured human endothelial cells for endothelial cell seeding of cardiovascular prosthetic device, particularly in small-diameter vascular grafts.

• Korean Journal of Ecology and Environment
• /
• v.40 no.1
• /
• pp.103-109
• /
• 2007

To investigate the effects of artificial vegetation island (AVI) on fish distribution, we compared fish fauna from artificial vegetation island (AVI) area, which installed in 2000, natural vegetation area (NVA) and vegetation-free area (VFA) at Kyungan Stream area of Lake Paldang from Jul. to Nov., 2005. Results showed that 11 families 23 genera 24 fish species were distributed in the AVI and NVA. Squalidus japonicus coreanus, a small-size fish which generally lives at the downstream, dominated absolutely in the individual numbers. However, only 6 families 11 genera 12 species of fishes caught at the VFA, and dominant fishes were Hemibarbus labeo and Erythroculter erythropterus, a medium to large-size migratory fishes which live in mid-depth of water column. The dominance index was high at the AVI (0.778) and NVA (0.868), whereas the diversity index and evenness index were high at the VFA. Fish distribution at AVI was similar to that of the NVA in numbers of species, indicating that the AVI could playa role as spawning and inhabitation zone to a variety of fishes. We believe that AVI may be used for a restoration of the damaged and disturbed littoral ecosystem.