• Journal of the Korean Society of Costume
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• v.54 no.8
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• pp.75-86
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• 2004

This study aims at considering and analyzing the stature of denim clothing as an ornament as well ai how aesthetic meaning and human being's mental side indwell in the development of denims. which will be proceeded in the future, from the view of contemporary culture of dressing. It is found that the stylishness expressed through denim clothing is formed on the basis of the cultures of party, drawing and disorganization, and the result of the study was as follows. Firstly, the culture of party became the source of for decoration of denim clothing, and denim clothing more glamorize women as a party-look which makes the most use of its advantage to be comfortable and able to display in various ways with splendid artificial jewelry, patchwork, dyed pattern which is elaborately embroidered. Secondly, Such culture of drawing is applied to denim clothing so that denims are expressed to make people feel more human being's warmth as being free from the existing stereotype and formality. Thirdly, the most outstanding feature of denim clothing showed in the culture of disorganization is to make the most use of vintage style as it is. This reflects an image of the culture of disorganization under postmodernism, which is free from the traditional conception of the existing dressing by destroying the original form, in the way of slashing, making a hole and tearing. That is, people can sufficiently express not only free sense of release based on postmodernism by wearing denim clothing, but also human being's intrinsic desire for restoration of humanism or human warmth with splendid decoration or various techniques such as handicraft. It can be recognized these features as the reasons, that make denim clothing place themselves as an original fashion item, by giving denim clothing technical decoration in recent years.

• Journal of Life Science
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• v.33 no.8
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• pp.612-622
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• 2023

T-cell deficiency may occur in various clinical conditions including congenital defects, cell/organ transplantation, HIV infection and aging. In this regard, the development of artificial thymus has recently been attracting much attention. To achieve this aim, the development of techniques for 3D culture of thymic stromal cells is necessary because thymocytes grown only in a 3D thymic microenvironment can be differentiated fully to become mature, immunocompetent T cells; the same cannot be achieved for thymocytes grown in 2D. This study aimed to develop a nanotechnology-based 3D culture technique using polymeric scaffolds for thymic epithelial cells (TECs), the main component of thymic stromal cells. Scanning electron microscopic observation revealed that the pores of both PCL and PCL/PLGA scaffolds were filled with TECs. Interestingly, TECs grown in 3D on polydopamine-coated scaffolds exhibited enhanced cell attachment and proliferation compared to those grown on non-coated scaffolds. In addition, the gene expression of thymopoietic factors was upregulated in TECs cultured in 3D on polydopamine-coated scaffolds compared to those cultured in 2D. Taken together, the results of the present study demonstrate an efficient 3D culture model for TECs using polymeric scaffolds and provide new insights into a novel platform technology that can be applied to develop functional, biocompatible scaffolds for the 3D culture of thymocytes. This will eventually shed light on techniques for the in vitro development of T cells as well as the synthesis of artificial thymus.

• Proceedings of the Korea Information Processing Society Conference
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• 2021.11a
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• pp.391-392
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• 2021

해마다 실내 냉방기 냉매누설 문제가 고질적으로 반복되며 소비자들의 피해도 커져가고 있다. 특히 제조사와 설치 업체가 다른 경우 냉매 누수의 원인이 제품인지, 설치하자인지 책임소재를 두고 갈등을 빚는 경우가 빈번하다. 이에 더 이상 소비자들의 피해를 막기 위해 냉매누설 검출 방안 마련이 필요해 보인다. 본 연구에서는 실내 냉방기 설치 후 냉매누설 검출을 위한 별도의 하드웨어 장치 추가 없이 냉방기의 운영을 위해 설치된 센서들의 값을 이용하여 냉매누설의 유무를 판단할 수 있는 방안을 제안하는 것을 목적으로 한다. 데이터 분석을 위하여 제조사의 제품 출하 전 현장 테스트 단계에서 측정한 온도값, 전류값, 습도값을 취합하여 데이터 셋을 구축하였다. 이때 자동화된 머신러닝(AutoML)을 이용하여 데이터의 80%를 훈련 데이터로 20%를 테스트 데이터로 사용하여 냉매량 80%는 1, 그 이하는 0으로 훈련시켰다. 구축한 데이터 셋을 이용하여 훈련시킨 결과 99% 정확도로 냉매누설 검출을 분별할 수 있었다. 또한 냉매누설과 관련성이 높은 중요 특징 4개를 추출할 수 있었다. 본 연구를 통하여 별도의 하드웨어 장치 추가 없이 소프트웨어적인 접근 방법으로 문제를 해결할 수 있는 feasibility를 확인할 수 있었다.

• The Journal of the Convergence on Culture Technology
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• v.10 no.4
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• pp.669-674
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• 2024

Up to now, domestic festival culture has been continued by traditional way, so it has been continued to the thema of local traditional culture after the stage of promoting the local agricultural products and marine products. Recently, a newscaster was replaced by the AI newscaster of using AI technology, and what was more AI technology is used to the election campaign. Also, in the university and local festival, VR and XR technologies have been used. To prove the fact that XR technologies could be used in the military training, XR technologies were applied to the military training program. It is expected that these VR and XR technologies will be applied to not only the military training, but also various areas, and change the festival culture with the new trend. In this study, we proved that the XR technologies could be applied to the festival culture.

• Journal of Plant Biotechnology
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• v.30 no.3
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• pp.269-273
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• 2003

In order to develop an efficient micropropagation protocal for Eucalyptus pellita, on in vitro culture system has been was established by inducing axillary buds from greenhouse stock materials. Among 6different media tested, DKW medium was the best ot induce bast induce both shoot proliferation and growth. Average number of proliferated shoots of 403per explant was obtained at the concentration of 0.1mg/LBA. Most of the stem materials excreted phenolic compounds at the proximal part of the explant and caused darking of the media. Therefore, it was necessary to transfer frequently to a fresh medium and/or to add activated charcoal at the concentration of 0.02％(w/v). Generally on vitro roots were formed easily on 1/2DKW medium with NAA treatment. All the explants rooted at the medium containing 0.2mg/L NAA and displayed vigorous root growth in vitro culture conditions. After transferred to an artificial soil mixture (peatmoss: vermiculrite: perlite, 1:1:1, v/v/v) in the greenhouse, most rooted plantlets survived well without any morphological abnormalities. The results show that the species can be micropropagated effectively by the application of axillary bud culture system.

• Microbiology and Biotechnology Letters
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• v.22 no.4
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• pp.415-422
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• 1994

The biodegradation of aromatic compounds by a mixed culture GE1 was investigated in an artificial wastewater containing 250 mg/l of benzene, toluene, and phenol in semicontinuous culture. In the control group (no strains) with an aeration rate of 75 ml/l/min, 37% of phenol and 83% of benzene were volatilized during early 24 hrs and toluene was disappeared from the medium within 12 hrs. The biodegradation of benzene and toluene was effective in SB (strains + biofilm) treatment, while phenol was degraded more quickly in SG (strains + glucose) treatment including glucose as an additional carbon source. aromatic compounds added at a concentration of 250 mg/l were completely removed by SG treatment after 16 hrs or 32 hrs, respectively. The removal rate of COD was high as much as 80 mg/l/h in SG treatment during early period, but COD revealed a stable value of 116~140 mg/l after 12 hrs caused by increased biomass. Therefore, it is concluded that the mixed GE1 could be used for the wastewater treatment including aromatic compounds such as benzene, toluene, and phenol.

• Clinical and Experimental Reproductive Medicine
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• v.49 no.4
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• pp.225-238
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• 2022

The ultimate goal of human assisted reproductive technology is to achieve a healthy pregnancy and birth, ideally from the selection and transfer of a single competent embryo. Recently, techniques for efficiently evaluating the state and quality of preimplantation embryos using time-lapse imaging systems have been applied. Artificial intelligence programs based on deep learning technology and big data analysis of time-lapse monitoring system during in vitro culture of preimplantation embryos have also been rapidly developed. In addition, several molecular markers of the secretome have been successfully analyzed in spent embryo culture media, which could easily be obtained during in vitro embryo culture. It is also possible to analyze small amounts of cell-free nucleic acids, mitochondrial nucleic acids, miRNA, and long non-coding RNA derived from embryos using real-time polymerase chain reaction (PCR) or digital PCR, as well as next-generation sequencing. Various efforts are being made to use non-invasive evaluation of embryo quality (NiEEQ) to select the embryo with the best developmental competence. However, each NiEEQ method has some limitations that should be evaluated case by case. Therefore, an integrated analysis strategy fusing several NiEEQ methods should be urgently developed and confirmed by proper clinical trials.

• International Journal of Advanced Culture Technology
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• v.12 no.1
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• pp.175-181
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• 2024

The paper examines the impact of digital media art on urban creative products, analyzing opportunities and challenges in the digital era. It emphasizes the development of urban cultural and creative products, highlighting their significance and future growth potential. The digital media era provides unprecedented innovation opportunities, utilizing advanced tools for efficient design, production, and marketing. Trends like personalization, customization, AI, and big data offer new expressions and market prospects. Cultural products evolve in design, marketing, and sales channels due to digital media, with tools like social media and e-commerce platforms opening new promotion avenues. Case studies illustrate digital media's role in driving innovation and enhancing user experiences. The paper addresses challenges in market competition, copyright, and technological renewal, while recognizing opportunities from AI and big data. The creative industries must adapt and innovate to remain relevant. Looking ahead, urban creative products will evolve under digitalization, relying on digital means to attract consumers and enhance brand value. Cultural products, beyond economic entities, disseminate urban culture and creative spirit. In the digital era, urban creative products demonstrate potential and necessity, prompting a reevaluation of digital technology's role. Through continuous innovation, this field contributes to cultural and economic levels, impacting urban characteristics and heritage. Urban creative products play an increasingly vital role in the global cultural and creative economy.

• Macromolecular Research
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• v.15 no.4
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• pp.370-378
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• 2007

Mechanical stimulation is known to activate several cellular signal transduction pathways, leading to the induction of signaling molecules and extracellular matrix (ECM) proteins, thereby modulating cellular activities, such as proliferation and survival. In this study, primary human dermal fibroblasts (HDFs) were seeded onto chitosan-based scaffolds, and then cultured for 3 weeks in a bioreactor under a cyclic strain of 1 Hz frequency. Compared to control samples cultured under static conditions, the application of a cyclic strain stimulated the proliferation of HDFs in I week, and by week 3 the thickness of the cell/scaffold composites increased 1.56 fold. Moreover, immunohistochemical staining of the culture media obtained from the cell/scaffold samples subjected to the cyclic strain, revealed increases in the expression and secretion of ECM proteins, such as fibronectin and collagen. These results suggest that the preconditioning of cell/scaffold composites with a cyclic strain may enhance the proliferation of HDFs, and even facilitate integration of the engineered artificial dermal tissue into the host graft site.

• Fisheries and Aquatic Sciences
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• v.1 no.1
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• pp.30-34
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• 1998

Rotifer culture fed on five types of artificial microparticle diets were evaluated to substitute the natural diets such as Chlorella or w-yeast. These microparticle diets including solidified blood using squid oil (SBSO), solidified blood using soybean oil (SBSB), nylon protein walled particle (NPW) simple coacervation oil capsule (SCO), complex coacervation oil capsule(CCO), were tested for the evaluation of feeding efficiency. The prepared micro particle diets had diameters ranging from 3 to 30 Jim. Rotifer culturing experiments were carried out in 3-liter beakers for 13-16 days. The initial inoculum density of rotifers was 10 ind./ml. The rotifers fed on Chlorella or $\omega-yeast$ showed maximal densities of 2,000 ind./ml in 9 days or 500 ind./ml in 7 days, respectively. Those fed on SBSO, SBSB or NPW showed maximal densities of 1568 ind./ml, 586 ind./ml or 503 ind./ml, respectively and the reproductive rates for those diets were equivalent to or better than w-yeast. However, the coacervated oil capsule showed lower maximal densities of 400 ind./ml for SCO and less than 100 ind./ml for CCO due to the unbalanced diet formulation and indigestibility.