• Journal of Environmental Impact Assessment
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• v.25 no.4
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• pp.233-247
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• 2016

The purpose of this study is to offer the basic data for management of National Park by surveying and analysing the change of flora of damaged lands in Juwangsan National Park, Korea. In cause of occurring the damaged lands, site-1 was the vegetation damage by stamping, the removing Gwangamsa of site-2, 3, 4 and the removing Naewon village of site-5, 6, 7. Whole sites are caused by the artificial disturbance and interference. The numbers of flora were summarized as 135 taxa including 52 families, 109 genera, 116 species, 2 subspecies and 17 varieties in whole sites. The status of flora by sites, site-6 is the largest number of 52 taxa, site-1 is the lowest of 23 taxa. The rare plant is 1 taxa, 4 taxa of endemic plants, 10 taxa of specific plants by floristic region, 9 taxa of naturalized plants and 1 taxa of invasive alien plant. In the results of analysis about the change of coverage ratio, for this shrub layer, site-7 was changed to the most $0%{\rightarrow}50%$, and the herb layer, site-5 was changed to the most $75%{\rightarrow}95%$. In case of the change of species numbers, most sites tended to increase in the sites introduced different species or generated by the growth. It is contemplated that is similar to the restoration with the surrounding natural vegetation that is in progress is a transition occurs with the passage of time. In the dominant species, the shrub layer is Fraxinus sieboldiana, Lespedeza bicolor, Rhus javanica, Lespedezamaximowiczii, Salix koreensis and Zelkova serrata, and Miscanthus sinensis var. purpurascens, Oplismenus undulatifolius, Artemisia princeps, Bothriochloa ischaemum and Erigeron annuus of herb layer. In the results of analysing Naturalized Index(NI) and Urbanized Index(UI), NI was increased or decreased this phenomenon with the lapse of time, UI was the most increase. In particular, the increase in UI is due to the transition process, the pioneering plant, naturalized plant was imported to continue.

• Asia pacific journal of information systems
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• v.18 no.1
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• pp.79-96
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• 2008

One of the roles of the Semantic Web services is to execute dynamic intra-organizational services including the integration and interoperation of business processes. Since different organizations design their processes differently, the retrieval of similar semantic business processes is necessary in order to support inter-organizational collaborations. Most approaches for finding services that have certain features and support certain business processes have relied on some type of logical reasoning and exact matching. This paper presents our approach of using imprecise matching for expanding results from an exact matching engine to query the OWL(Web Ontology Language) MIT Process Handbook. MIT Process Handbook is an electronic repository of best-practice business processes. The Handbook is intended to help people: (1) redesigning organizational processes, (2) inventing new processes, and (3) sharing ideas about organizational practices. In order to use the MIT Process Handbook for process retrieval experiments, we had to export it into an OWL-based format. We model the Process Handbook meta-model in OWL and export the processes in the Handbook as instances of the meta-model. Next, we need to find a sizable number of queries and their corresponding correct answers in the Process Handbook. Many previous studies devised artificial dataset composed of randomly generated numbers without real meaning and used subjective ratings for correct answers and similarity values between processes. To generate a semantic-preserving test data set, we create 20 variants for each target process that are syntactically different but semantically equivalent using mutation operators. These variants represent the correct answers of the target process. We devise diverse similarity algorithms based on values of process attributes and structures of business processes. We use simple similarity algorithms for text retrieval such as TF-IDF and Levenshtein edit distance to devise our approaches, and utilize tree edit distance measure because semantic processes are appeared to have a graph structure. Also, we design similarity algorithms considering similarity of process structure such as part process, goal, and exception. Since we can identify relationships between semantic process and its subcomponents, this information can be utilized for calculating similarities between processes. Dice's coefficient and Jaccard similarity measures are utilized to calculate portion of overlaps between processes in diverse ways. We perform retrieval experiments to compare the performance of the devised similarity algorithms. We measure the retrieval performance in terms of precision, recall and F measure? the harmonic mean of precision and recall. The tree edit distance shows the poorest performance in terms of all measures. TF-IDF and the method incorporating TF-IDF measure and Levenshtein edit distance show better performances than other devised methods. These two measures are focused on similarity between name and descriptions of process. In addition, we calculate rank correlation coefficient, Kendall's tau b, between the number of process mutations and ranking of similarity values among the mutation sets. In this experiment, similarity measures based on process structure, such as Dice's, Jaccard, and derivatives of these measures, show greater coefficient than measures based on values of process attributes. However, the Lev-TFIDF-JaccardAll measure considering process structure and attributes' values together shows reasonably better performances in these two experiments. For retrieving semantic process, we can think that it's better to consider diverse aspects of process similarity such as process structure and values of process attributes. We generate semantic process data and its dataset for retrieval experiment from MIT Process Handbook repository. We suggest imprecise query algorithms that expand retrieval results from exact matching engine such as SPARQL, and compare the retrieval performances of the similarity algorithms. For the limitations and future work, we need to perform experiments with other dataset from other domain. And, since there are many similarity values from diverse measures, we may find better ways to identify relevant processes by applying these values simultaneously.

• Korean Journal of Veterinary Research
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• v.36 no.3
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• pp.681-692
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• 1996

The present study was conducted to isolate Babesia gibsoni by culture method of the microaerophilous stationary phase(MASP) and analyse the antigenic properties of the parasite by SDS-PAGE and immunoblot. The results obtained were summarized as follows. The protozoan parasite Babesia gibsoni multiplied in canine erythrocytes in RPMI 1640 medium(pH7.0) containing 20 40% normal canine serum under the MASP condition of 5% CO2 and 95% air at $37^{\circ}C$ incubator. The levels of parasitaemia in the erythrocytes were shown more higher by exchanging the medium at 24 hours interval. Under the above condition of MASP, the percentage of parasitized erythrocytes(PPE) after incubation for 8 days increased about 14 times more than that in the initiation of the 1% infected canine erythrocyte culture. The parasites were purely isolated from the MASP culture of red blood cells collected from dogs infected with Babesia gibsoni naturally or artificially. Among the total of 36 canine(Pit-bullterier) blood samples the parasites were isolated from 17 cases(47.2%) in the MASP culture while the parasites were detected from 20 cases(56%) and 12 cases(33.3%), respectively, by indirect fluorescent antibody(IFA) test and direct light microscopy(DLM). On the other hand, Babesia gibsoni was isolated by MASP culture from 15 cases(75%) and 11 cases(92%) of positive cases of IFA and DLM, respectively. In the analysis of the erythrocytic merozoite(AEOM) antigen derived from infected dog approximately 11 antigenic bands in molecular weight of 130, 120, 97.4, 92, 80, 52, 50, 42, 36, 30 and 29 KDa were observed on SDS-PAGE. Antigenic bands in the endoerythrocytic merozoite(CEOM) antigen derived from infected erythrocyte (sediment) in MASP culture were much similar to those of AEOM bands. In the exoerythrocytic merozoite(CEEM) antigen derived from supernatant of the infected erythrocyte culture approximately 20 antigenic bands were observed and the molecular weight of the major bands among these were 140, 120, 114, 105, 96, 93, 92, 80, 60, 52, 50, 38, 36, 30, 24, 18.5 and 16 KDa. In the protein patterns of AEOM and CEOM antigen by immunoblot 15 bands were observed and these patterns were much similar between each other. The molecular weight of the major bands in the both antigens were 130, 120, 80, 60, 52, 50, 42, 30, 29, 18.5 and 16 KDa. Approximately 21 bands were observed in CEEM antigen and the molecular weight of the major bands were 140, 120, 96, 92, 85, 80, 76, 60, 52, 50, 37, 30, 24, 16 and 15 KDa. The specific antigenic bands in the artificially infected dogs were firstly observed at 3 weeks afrer inoculation of infected blood and these antigenic bands were maintained up to 18 months after inoculation. In the immunoblot of the sera of the splenectomized dogs the specific antigenic bands with the molecular weight of 93 KDa and 52 KDa, respectively, were observed weakly comparing to those of non-splenectomized dog. In immunoblot of the sera collected from the naturally infected dogs the antigenic bands were observed as same as those of artificially infected dogs while antigenic band of 29 KDa in some individual dog showed strongly. In comparison of immunoblot of the sera collected from dogs non-treated and treated with diminazene aceturate(7mg/kg, IM) after artificial infection no differences of antigenic bands were observed. In analysis of antigenic bands by digoxigenin glycan/protein double labeling, antigenic bands in the molecular weight of 106, 60 58, 36, 30 and 29 KDa were determined as glycoproteins.

• Journal of Mushroom
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• v.18 no.1
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• pp.1-9
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• 2020

Mycorrhiza refers to the association between a plant and a fungus colonizing the cortical tissue of the plant's roots during periods of active plant growth. The benefits afforded by plants from mycorrhizal symbioses can be characterized either agronomically, based on increased growth and yield, or ecologically, based on improved fitness (i.e., reproductive ability). In either case, the benefit accrues primarily because mycorrhizal fungi form a critical linkage between plant roots and the soil. The soilborne or extramatrical hyphae take up nutrients from the soil solution and transport them to the root. This mycorrhizae-mediated mechanism increases the effective absorptive surface area of the plant. There are seven major types of mycorrhizae along with mycoheterotrophy: endomycorrhizae (arbuscular mycorrhizae, AM), ectomycorrhizae (EM), ectendomycorrhizae, monotropoid, arbutoid, orchid, and ericoid. Endomycorrhizal fungi form arbuscules or highly branched structures within root cortical cells, giving rise to arbuscular mycorrhiza, which may produce extensive extramatrical hyphae and significantly increase phosphorus inflow rates in the plants they colonize. Ectomycorrhizal fungi may produce large quantities of hyphae on the root and in the soil; these hyphae play a role in absorption and translocation of inorganic nutrients and water, and also release nutrients from litter layers by producing enzymes involved in mineralization of organic matters. Over 4,000 fungal species, primarily belonging to Basidiomycotina and to a lesser extent Ascomycotina, are able to form ectomycorrhizae. Many of these fungi produce various mushrooms on the forest floor that are traded at a high price. In this paper, we discuss the benefits, nutrient cycles, and artificial cultivation of mycorrhizae in Korea.

• Journal of Mushroom
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• v.18 no.1
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• pp.45-52
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• 2020

Termitomyces albuminosus has been recognized to have the best mushrooms in China, in terms of taste and aroma. The efficacy of these mushrooms has been recorded in the botanical list. However, research on the development of their artificial culture methods is necessary. In this study, we prepared an organic solvent extract and a hot water extract to understand the development of compounds and functional foods with antioxidant and anti-inflammatory activities. The IC₅₀ value of DPPH radical scavenging activity of the hot water extract (TA4) was 1.5 mg/mL and the IC₅₀ value of the MeOH fraction (TA2) was 1.93 mg/mL. The anti-inflammatory activity was investigated by the inhibition of NO production. EtOAc fraction (TA1) is a crude extract, but 79% of NO production was inhibited at 100 ㎍/mL. NO was not produced at 200 ㎍/mL. TA1-5-6, from TA1 inhibited NO production by 15% as compared to the positive control at 15 ㎍/mL, and completely inhibited NO production at 30 ㎍/mL. No cytotoxicity was observed at 50 ㎍/mL. TA2-1-5 from the MeOH fraction (TA2) inhibited more than 75% of NO production at 30 ㎍/mL; cytotoxicity was very low even at 50 ㎍/mL. In conclusion, by selective solvent selection, it was possible to manufacture an extract with no cytotoxicity and excellent biological activities. Furthermore, the extracts showed potential for developing various functional foods and drugs.

• Journal of The Korean Society of Grassland and Forage Science
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• v.6 no.1
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• pp.38-43
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• 1986

This field experiment was conducted to determine the effects of shading degrees (0 : full sunlight, 25, 50 and 75%) on the early growth characteristics and winter survival of grasses. For the test different artificial shading houses were established and pasture species used were orchardgrass, timothy and perennial ryegrass. The experiment was performed at Livestock Experiment Station in Suweon, and sowing date was Aug. 25, 1984. The results are summarized as follows : 1. Generally, root length, new root length, number of new root and tillers of grasses were increased at 0% (full sunlight) of shading, followed by 25, 50 and 75% of shading degrees, in that order. Plant length and leaf elongation, however were increased at 50% of shading, followed by 75, 25 and 0%. 2. The percentage of winter killing of grasses was significantly (P<0.05) increased as the shading degrees increased. Also the percentages of winter killing were 10.1% in orchardgrass, 9.5% in timothy and 14.2% in perennial ryegrass, respectively. 3. A significant negative correlations were observed in the relationship between winter killing and tillers, new root length and number of new root of grasses. However, there were positive correlations between winter killing and plant length or leaf elongation under shading conditions. 4. As the shading degrees are increased by 0, 25, 50 and 75%, the NAR of grasses was tended to be decrease to 10.98, 11.52, 9.81 and 6.12 g/$m^2$/day, in that order. Also there was a negative correlation (r=-0.5943*) between winter killing and NAR of grasses, and NAR of orchardgrass, timothy and perennial ryegrass were 8.58, 8.53 and 11.71 g/$m^2$/day, respectively.

• Korean Journal of Ecology and Environment
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• v.48 no.4
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• pp.253-262
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• 2015

The sustainability of Cypripedium japonicum, a rare plant designated by the Korea Forest Service, is threatened due to artificial factors such as habitat loss and climate change etc. and internal factors such as changes in biological properties of the habitat etc. but conservation research has not been performed in South Korea. The objective of this study is to establish the species conservation strategies by analyzing the characteristics of their habitats, including: 1) Population characteristics, and 2) habitat analysis of the vegetation and abiotic environments. From April to September, 2014, population characteristics [density (stems $m^{-2}$), flowering rate (%), and leaf area ($cm^2$)] in Cypripedium japonicum habitats such as Chuncheon (CC), Hwacheon (HC), Muju (MJ), and Gwangyang (GY) and vegetation characteristics (plant sociological research and ordination analysis), and abiotic environments [temperature ($^{\circ}C$), relative humidity (%), transmitted light ($mol{\cdot}m^{-2}{\cdot}d^{-1}$) and canopy openness (%)] were measured. Cypripedium japonicum was mainly distributed at elevation 450 to 990 m and 5 to $30^{\circ}$ slope. Slope direction was shown as 0 to $110^{\circ}$. Habitats temperature (mean $18.94^{\circ}C$) was well matched to seasonal changes. Differences among sites showed greater level according to latitude difference. It showed the highest in habitat, GY located in the South. On the other hand, relative humidity (77.38%) didn't show much difference among sites. The average degree of canopy openness was 18.17%. It showed the highest at HC (22.1%) and the lowest at MJ (16.1%). The average degree of transmitted light was $9.1mol{\cdot}m^{-2}{\cdot}d^{-1}$. It showed the highest at CC ($10.6mol{\cdot}m^{-2}{\cdot}d^{-1}$) and the lowest at GY ($6.87mol{\cdot}m^{-2}{\cdot}d^{-1}$). Chlorophyll content showed average 26.12 SPAD. It showed the highest at MJ (30.64 SPAD value) and the lowest at HC (23.69 SPAD value). Leaf area was average $253.35cm^2$. It showed the highest at CC ($281.51cm^2$) and the lowest at HC ($238.23cm^2$).

• Korean Journal of Ecology and Environment
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• v.46 no.3
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• pp.449-459
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• 2013

Forest vegetation of Geochilbong (1,177 m) in Deogyusan National Park is classified into mountain forest vegetation and flatland forest vegetation. Mountain forest vegetation is subdivided into deciduous broad-leaved forest, valley forest, coniferous forest, afforestation and etc., while riparian forest was found under the category of flatland forest vegetation. Including 89 communities of mountain forest vegetation and 4 communities of other vegetation, the total of 93 communities were researched; the distributed colonies classified by physiognomy classification are 32 communities deciduous broadleaved forest, 21 communities of valley forest, 12 communities of coniferous forests, 24 afforestation and 4 other communities. As for the distribution rate for surveyed main communities, Quercus mongolica, Quercus serrata, Quercus variabilis communities account for 56.54 percent of deciduous broad-leaved forest, Fraxinus mandshurica, Cornus controversa community takes up 46.58 percent of mountain valley forest, Pinus densiflora community holds 74.98 percent of mountain coniferous forest holds. In conclusion, minority species consisting of Quercus mongolica, Quercus serrata, Quercus variabilis, Fraxinus mandshurica, Cornus controversa, Pinus densiflora are distributed as dominant species of the uppermost part in a forest vegetation of Geochilbong in Deogyusan National Park. In addition, because of vegetation succession and climate factors, numerous colonies formed by the two species are expected to be replaced by Quercus mongolica, Carpinus laxiflora and Fraxinus mandshurica which are climax species in the area. However, the distribution rate of deciduous broad-leaved forest seems to increase gradually due to global warming and artificial disturbance.

• Journal of Embryo Transfer
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• v.18 no.2
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• pp.115-124
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• 2003

The objective of this study was to compare the effect of four different estrus induction methods in anestrus cows on the estrus induction and pregnancy following artificial insemination (AI). Sixty-five cows (3∼4 years old) were selected and divided into four different estrus induction treatment groups. Group 1, 12 cows were treated by Ovsynch program combined with GnRH and PGF$_2$a. Group 2, 12 cows were treated by "Tow plus Two" program with GnRH and PGF$_2$a. Group 3, 20 cows were treated by "Tow plus Two" program following intravaginal progesterone implantation (CIDR). Twenty one cows in Group 4 were treated by "Tow plus Two" program following follicular rupture and intravaginal progesterone implantation. Cows were then observed estrus induction and inseminated artificially at 12 h and 24 h after standing estrus. The rates of estrus induction in Group 4 (18/21, 86%) was significantly (P<0.05) higher than those in groups 1, 2 and 3 (8/12, 67%; 9/12, 75%; 14/20, 70%). In the mean time of onset of estrus after final administration of GnRH in different hormone-treated cows, the cows in Group 3 (24.2$\pm$2.2) and Group 4 (23.4$\pm$2.0) were significantly (P<0.05) shorter than that in Group 1 (28.5$\pm$4.6) and Group 2 (26.4$\pm$3.3). The rates of pregnancy diagnosed on Day 28 were significantly different between treatment groups. Significantly (P<0.05) higher rate of pregnancy was observed in Group 4 (17/20, 85.0%) than those in Groups 1, 2 and 3 (7/11, 63.6%; 8/12, 66.7%; 15/20, 75.0%, respectetively). The rate of abortion diagnosed on 49 days of gestation was significantly (P<0/05) lower in Group 4 (1/17, 5.9%) than those in Groups 1, 2 and 3 (2/7, 28.7%; 2/8, 25% and 3/15, 20%, respectively). In conclusion, combined treatments with GnRH and PGF$_2$a following follicular rupture and progesterone implant in anestrus cows was considered to be most effective in estrus induction and maintenance of pregnancy. Further studies are needed to verify the functional mechanisms of residual follicles in anestrus ovaries on retarding the response of hormonal treatments.sponse of hormonal treatments.

• Korean Journal of Clinical Laboratory Science
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• v.49 no.4
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• pp.350-358
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• 2017

The hemolysis index (HI) is semi-quantitative marker for hemolysis. Because the characteristics of the HI vary from one commercial platform to another, no standardization or harmonization of the HI is currently available. Specimens (N=40) randomly selected from clinical patients were artificially hemolyzed in vitro. The serum of the specimens was then diluted with a 20 mg/dL difference between 0~300 mg/dL based on serum hemoglobin measured using the XE-2100 hematology automation equipment (Sysmex, Japan). Diluted serum was measured using the Hitachi-7600 biochemical automation equipment (Hitachi, Japan) to differentiate between HI and serum hemoglobin. The data showed linearity between HI and serum hemoglobin and that HI 1 contained approximately 20 mg/dL of serum hemoglobin. To determine the blood rejection threshold, the HI was divided into three groups: HI 0~1, HI 4~6, HI 9~15. After another batch of clinical specimens (N=40) was measured using a Hitachi-7600 (Hitachi, Japan), each specimen was moved forward and backward with the piston of the syringe to induce an artificial in vitro hemolysis, then measured again with a Hitachi-7600 (Hitachi, Japan). The percentage difference between the three groups was analyzed by ANOVA or the Kruskal-Wallis test. In the post-test, there were significant differences between the HI 0~1 and the HI 5~6: Glucose, creatinine, total protein, AST, direct bilirubin, uric acid, phosphorus, triglyceride, LDH, CPK, Magnesium, and potassium levels. Because many clinical tests differed significantly, the threshold for hemolysis could be appropriate for HI 5 (serum hemoglobin 100 mg/dL).