한국버섯학회지 (Journal of Mushroom)

  • 제18권1호
  • /
  • Pages.45-52
  • /
  • 2020
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  • 1738-0294(pISSN)
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  • 2288-8853(eISSN)
한국버섯학회 (The Korean Society of Mushroom Science)
권호 표지
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계종버섯 추출물과 분획물의 라디칼 소거 활성과 항염증 활성 분석

Investigation of radical scavenging and anti-inflammatory activity of the extract and fractions of Termitomyces albuminosus

  • 강민재 (부경대학교 미생물학과) ;
  • 김영재 (부산대학교 생명환경화학과) ;
  • 황현정 (부산대학교 생명환경화학과) ;
  • 김문호 (부산대학교 생명환경화학과) ;
  • 신종무 (농업회사법인 (주)힘찬) ;
  • 신평균 (농업회사법인 (주)힘찬) ;
  • 김군도 (부경대학교 미생물학과) ;
  • 김근기 (부산대학교 생명환경화학과)
  • Kang, Min-Jae (Department of Microbiology, College of Natural Sciences, Pukyong National University) ;
  • Kim, Young-Jae (Department of Life Science and Environmental Biochemistry, College of Natural Resource and Life Sciences, Pusan National University) ;
  • Hwang, Hyeon-Jeong (Department of Life Science and Environmental Biochemistry, College of Natural Resource and Life Sciences, Pusan National University) ;
  • Kim, Moon-Ho (Department of Life Science and Environmental Biochemistry, College of Natural Resource and Life Sciences, Pusan National University) ;
  • Sin, Jong-Moo (Himchan Agriculture Co. Ltd.) ;
  • Shin, Pyung-Gyun (Himchan Agriculture Co. Ltd.) ;
  • Kim, Gun-Do (Department of Microbiology, College of Natural Sciences, Pukyong National University) ;
  • Kim, Keun Ki (Department of Life Science and Environmental Biochemistry, College of Natural Resource and Life Sciences, Pusan National University)
  • 투고 : 2020.03.13
  • 심사 : 2020.03.26
  • 발행 : 2020.03.31
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초록

계종버섯은 중국에서 맛과 향에서 최고의 버섯으로 평가받고 있으며, 본초강목에 그 효능이 기록되어 전해지고 있으나 효능과 물질연구 및 인공재배법 개발이 요구되고 있는 상황이다. 항산화 활성과 항염증 활성을 가지는 기능성 물질의 확보와 기능성식품 개발을 위한 기초자료를 확보하기 위하여 유기용매 추출물과 열수 추출물을 제조하고, 활성이 높은 분획물을 얻고자 하였다. CH2Cl2와 MeOH 혼합용매로 추출한 다음 비극성용매와 극성용매로서 용해성에 따라 분획하고, 열수 추출로 추출물을 확보하였다. 분획물과 추출물을 이용한 항산화 활성 조사에서는 열수 추출물(TA4)의 DPPH 라디칼 소거능에 대한 IC50값이 1.5 mg/mL로 나타났고, MeOH 분획물(TA2)에서는 IC50값이 1.93 mg/mL로 높게 나타났다. NO생성 억제율로 조사한 항염증 활성은 EtOAc 분획물(TA1)은 조추출물이지만 100 ㎍/mL 처리농도에서는 79% NO생성이 억제되고, 200 ㎍/mL 농도에서는 NO가 전혀 생성되지 않았다. TA1 물질을 2차례 TLC로 분리한 TA1-5-6물질은 15 ㎍/mL 처리농도에서 대조구와 비교하여 NO생성이 86% 억제되었고, 30 ㎍/mL 농도에서는 NO 생성이 완전히 억제되었다. 그리고 세포독성 실험에서는 50 ㎍/mL 농도에서도 전혀 독성이 나타나지 않았다. MeOH 분획물(TA2) 유래의 TA2-1-5물질은 30 ㎍/mL 처리농도에서 75% 이상 NO생성이 억제되었으며, 세포 독성은 50 ㎍/mL에서도 아주 낮게 나타났다. 선택적 용매의 선별과 추출 방법의 개발을 통하여 세포 독성이 없고, 생리활성이 뛰어난 계종버섯 분획물 확보가 가능하였고, 기능성식품 및 건강 기능성소재로서의 충분한 잠재력을 확인할 수 있었다.

Termitomyces albuminosus has been recognized to have the best mushrooms in China, in terms of taste and aroma. The efficacy of these mushrooms has been recorded in the botanical list. However, research on the development of their artificial culture methods is necessary. In this study, we prepared an organic solvent extract and a hot water extract to understand the development of compounds and functional foods with antioxidant and anti-inflammatory activities. The IC50 value of DPPH radical scavenging activity of the hot water extract (TA4) was 1.5 mg/mL and the IC50 value of the MeOH fraction (TA2) was 1.93 mg/mL. The anti-inflammatory activity was investigated by the inhibition of NO production. EtOAc fraction (TA1) is a crude extract, but 79% of NO production was inhibited at 100 ㎍/mL. NO was not produced at 200 ㎍/mL. TA1-5-6, from TA1 inhibited NO production by 15% as compared to the positive control at 15 ㎍/mL, and completely inhibited NO production at 30 ㎍/mL. No cytotoxicity was observed at 50 ㎍/mL. TA2-1-5 from the MeOH fraction (TA2) inhibited more than 75% of NO production at 30 ㎍/mL; cytotoxicity was very low even at 50 ㎍/mL. In conclusion, by selective solvent selection, it was possible to manufacture an extract with no cytotoxicity and excellent biological activities. Furthermore, the extracts showed potential for developing various functional foods and drugs.

키워드

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