• Title/Summary/Keyword: ars-1

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Transcription level of the ars-1 promoter of Neurospora crassa (Neurospora crassa ars-1 프로모터의 발현율 조사)

  • 이병욱;구상호
    • Journal of Life Science
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    • v.13 no.2
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    • pp.191-196
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    • 2003
  • The ars gene of the Neurospora crassa encodes arylsulfatase and is expressed under sulfur limitation. An ars-1 promoter(Pars) translationally-fused to a lacZ gene was transformed into the N. crassa RLM 35-35, a his-3 inl strain and integrated into the his-S locus by a single crossover homologous recombination. $\beta$-galactosidase specific activity was measured from mycelia grown in sulfur-limited Vogel's medium. Enzyme activity reached its maximum at 14 hour after the shift to derepressing condition. When activity from homokaryon generated by microconidiation was measured, it was 17% a higher than that from heterokaryon.

Isolation and ars Detoxification of Arsenite-Oxidizing Bacteria from Abandoned Arsenic-Contaminated Mines

  • Chang, Jin-Soo;Yoon, In-Ho;Kim, Kyoung-Woong
    • Journal of Microbiology and Biotechnology
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    • v.17 no.5
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    • pp.812-821
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    • 2007
  • The ecosystems of certain abandoned mines contain arsenic-resistant bacteria capable of performing detoxification when an ars gene is present in the bacterial genome. The ars gene has already been isolated from Pseudomonas putida and identified as a member of the membrane transport regulatory deoxyribonucleic acid family. The arsenite-oxidizing bacterial strains isolated in the present study were found to grow in the presence of 66.7 mM sodium arsenate($V;\;Na_2HAsO_4{\cdot}7H_2O$), yet experienced inhibited growth when the sodium arsenite($III;\;NaAsO_2$) concentration was higher than 26 mM. Batch experiment results showed that Pseudomonas putida strain OS-5 completely oxidized 1 mM of As(III) to As(V) within 35 h. An arsB gene encoding a membrane transport regulatory protein was observed in arsenite-oxidizing Pseudomonas putida strain OS-5, whereas arsB, arsH, and arrA were detected in strain OS-19, arsD and arsB were isolated from strain RW-18, and arsR, arsD, and arsB were found in E. coli strain OS-80. The leader gene of arsR, -arsD, was observed in a weak acid position. Thus, for bacteria exposed to weak acidity, the ars system may cause changes to the ecosystems of As-contaminated mines. Accordingly, the present results suggest that arsR, arsD, arsAB, arsA, arsB, arsC, arsH, arrA, arrB, aoxA, aoxB, aoxC, aoxD, aroA, and aroB may be useful for arsenite-oxidizing bacteria in abandoned arsenic-contaminated mines.

Normative Study of the K-ARS(Korean ADHD Rating Scale) for Parents (한국어판 주의력결핍 과잉행동장애 평가척도의 부모용 규준연구)

  • Jang, Su-Jin;Suh, Dong-Su;Byun, Hee-Jung
    • Journal of the Korean Academy of Child and Adolescent Psychiatry
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    • v.18 no.1
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    • pp.38-48
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    • 2007
  • Objectives : The K-ARS (Korean ADHD Rating Scale) is one of the most important assessment tool of attention-deficit hyperactivity disorder (ADHD) in Korea. in this study, we presented detailed normative data on the K-ARS for school-aged children in Seoul metropolitan area to put it to practical use. Methods : The subjects were 2,397 students(1,223 boys and 1,174 girls, aged 6-12) from 4 elementary schools in Seoul, and one caretaker of each child completed the K-ARS for parents. Children who showed high scores of the K-ARS for parents were screened, and 2 child psychiatrists interviewed them to make a clinical diagnosis. We compared the mean scores of the K-ARS for parents between ADHD and normal group, and examined the percentage of correct classification. Results : There were some differences in score of the K-ARS for parents according to sex and age, so we presented continuous normative data with T score and subdivided cut-off points for ADHD screening. Interviews with child psychiatrists using DSM-IV criteria were performed to test diagnostic validity, and the difference in every the K-ARS for parents index between ADHD and normal group was significant(p<.001). Using 3 different cut-of points(80th, 90th, 93rd percentage), the accuracies of ADHD correct classification were 67.9, 72.2, 71.1% and all 3 canonical discriminants were significant (p<.05) between ADHD and normal group. Conclusion : The normative data and cut-off points on the K-ARS for parents are useful in screening ADHD children in Seoul metropolitan area.

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TREATMENT OF CLASS II DIVISION 1 MALOCCLUSION WITH L-ARS(LIGATED ANTERIOR REPOSITIONING SPLINT) (L-ARS를 이용한 Cl II div. 1 부정교합의 치험례)

  • Kim, Jong-Chul;Williamson, Eugene H.
    • The korean journal of orthodontics
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    • v.23 no.4 s.43
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    • pp.447-454
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    • 1993
  • Patient with skeletal class II relationship was treated with L-ARS. L-ARS is fixed functional appliance that could be effective in children and adolescent patient who don't wear activator. The following results were obtained ; 1) Growth of Mandible was stimulated and overjet was decreased, therefore coupling of anterior teeth was established with L-ARS which is fixed functional appliance. 2) These changes were accomplished with Mandibular skeletal growth with no effect on the Maxilla. 3) L-ARS was especially effective on patient who deny to wear the removable functional appliance.

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Selection of optimum medium for mass production of Metarhizium anisopliae ARS 978 (Metarhizium anisopliae ARS 978의 대량 배양을 위한 배지 조건)

  • Moon, Ki-Hyuk;Yoon, Jeong-Weon;Yoon, Cheol-Sik;Kim, Seung-Wook
    • The Korean Journal of Pesticide Science
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    • v.2 no.2
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    • pp.90-96
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    • 1998
  • The purpose of this study is to investigate the liquid culture conditions for mass production of Metarhizium anisopliae var. majus ARS 978 which is a potential microbiological pesticide. The temperature and pH range for optimal cultivation were $28^{\circ}C$ and pH 7.0, respectively. For M. anisopliae ARS 978, 1.0 %(v/v) molasses, 1.0 %(w/v) distiller's dry solubles, and 0.3 %(w/v) $CaCO_{3}$ were found to be the proper nutrients, considering cell mass, enzyme activities and spore concentration.

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Performance Improvement of a Temperature and Humidity Measuring System for Strawberry Cultivation Greenhouse (딸기재배 온실용 온습도 계측시스템의 성능개선)

  • Jeong, Young Kyun;Lee, Jong Goo;Ahn, Enu Ki;Seo, Jae Seok;Yoon, Yong Cheol
    • Journal of Bio-Environment Control
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    • v.29 no.2
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    • pp.110-119
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    • 2020
  • This study investigates the improvement in the performance of a temperature and humidity measuring system developed by Company A using the Aspirated Radiation Shield (ARS). The shield has been used in the industry and its accuracy was verified recently. The study also experimentally examines the impact of the wind speed of the ARS device on temperature and humidity. The results are summarized as follows. Before the improvement of the system, the temperature of Company A's system was up to 10.2℃ higher than that measured by the ARS device, and the measured relative humidity was approximately 20.0% lower. After improving the system, the temperature and relative humidity of nodes 1 and 2 were found to be almost identical. The temperature deviations including the maximum, mean, and minimum temperatures between those measured in node 2 and by ARS were approximately 0.2 to 0.7℃, respectively; further, the values measured by ARS tended to be slightly lower or higher. In terms of relative humidity, the ARS measurements yielded values approximately 10.0% higher immediately after sunset; otherwise, the values were approximately 1.9% lower. Moreover, when node 1 was set to minimum-middle, middle-maximum, and maximum, the deviations including the maximum, mean, and minimum temperatures of nodes 1 and 2 were 0.1 to 0.4℃, 0.0 to 0.2℃, and 0.0 to 0.5℃, respectively. The deviations including the maximum, average, and minimum temperatures of the three points of node 1 and the ARS ranged from 0.2 to 0.5℃, 0.1 to 2.2℃, and 0.1 to 1.1℃, respectively, indicating that the temperature deviation according to the wind speed was negligible. In addition, considering the results of the previous study with those from this study, the optimal wind speed to improve the temperature deviation is considered to be in the range of 1.0~2.0 m·s-1.

Comparison of Tibial Tuberosity Transposition and Anti-rotation Suture for Medial Patellar Luxation in 133 Small-breed Dogs

  • Son, Chansoo;Cheong, Jongtae;Lee, Joo Myoung
    • Journal of Veterinary Clinics
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    • v.35 no.5
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    • pp.200-205
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    • 2018
  • This retrospective study is designed to compare the clinical results of tibial tuberosity transposition (TTT) and anti-rotation suture (ARS) treatments for medial patellar luxation (MPL). Medical records of 133 dogs were reviewed that had undergone surgical correction of MPL between January 2013 and May 2017. MPL correction was performed on 182 stifles, with TTT and ARS being performed on 101 stifles and 81 stifles. The common dog breeds receiving surgical treatment for MPL were Maltese, Pomeranian, Chihuahua and Poodle. Mean age of dogs with MPL was 32.6 months, and their mean body weight was 4.26 kg. Seventy dogs (52.6%) were male and 63 (47.4%) were female. Of the 182 stifles with MPL, grade II, III and IV were 18.7%, 72.0% and 9.3%. Total complications after TTT and ARS were recorded in 16.8% and 29.6%. Major complications after TTT and ARS were recorded in 5.9% and 12.3%, minor complications after TTT and ARS were recorded in 10.9% and 17.3%. The risk of complication and reluxation rate after TTT were significantly lower than that for ARS (p < 0.05). However, the rate of reluxation among dogs treated by ARS (1.2%) for grade II MPL was significantly lower than that for dogs treated by ARS (8.6%) for grade III MPL (p < 0.05). The TTT group had a shorter recovery period after surgical intervention than that in the ARS group (p < 0.001). In conclusion, TTT had a significantly lower incidence of complication and a shorter recovery period than ARS. However, ARS for grade II MPL appears to be a good surgical option for reducing the rate of reluxation after surgery. These results of this study could be used to provide therapeutic guidelines for surgical MPL correction in small-breed dogs.

A Study on Pose Control for 2 wheel Robot using ARS (ARS를 이용한 2바퀴 로봇의 자세 제어에 관한 연구)

  • Kang, Jin Gu
    • Journal of Korea Society of Digital Industry and Information Management
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    • v.9 no.1
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    • pp.73-78
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    • 2013
  • In this paper, configuration control for the Horizontal Maintenance of the 2 wheel robot has been studied using ARS(Attitude Refrence System). The 2 wheel robot technique is getting attention and there have been many researches on the seg-way since the US. Using its 2 freedom, a 2 wheel robot can move in various modes and Our robot performs goal reaching ARS.2 wheel robot fall down to the forward or reverse direction to converge to the stable point. Kalman Filter is normally used for the algorithm and numerous research is progressing at the moment. To calculate the attitude in ARS using 2 axis gyro(roll, pitch) and 3 axis accelerometers (x, y, z). In this paper we present a two wheel robot system for an autonomous mobile robot. This paper realized the robot control method which is much simpler but able to get desired performance by using the ARS control.

A Study on Integrated Control of AFS and ARS Using Fuzzy Logic Control Method (Fuzzy Logic 제어를 이용한 AFS와 ARS의 통합제어에 관한 연구)

  • Song, Jeonghoon
    • Transactions of the Korean Society of Automotive Engineers
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    • v.22 no.1
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    • pp.65-70
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    • 2014
  • An Integrated Dynamics Control system with four wheel Steering (IDCS) is proposed and analysed in this study. It integrates and controls steer angle of front and rear wheel simultaneously to enhance lateral stability and steerability. An active front steer (AFS) system and an active rear steer (ARS) system are also developed to compare their performances. The systems are evaluated during brake maneuver and several road conditions are used to test the performances. The results showed that IDCS vehicle follows the reference yaw rate and reduces side slip angle very well. AFS and ARS vehicles track the reference yaw rate but they can not reduce side slip angle. On split-${\mu}$ road, IDCS controller forces the vehicle to go straight ahead but AFS and ARS vehicles show lateral deviation from centerline.

Electrochemical Studies on $Sm^3+$-ARS Complexes ($Sm^3+$-ARS착물의 전기화학적 연구)

  • Son, Byoung-Chan
    • The Journal of Natural Sciences
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    • v.11 no.1
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    • pp.33-39
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    • 1999
  • Electrochemical behaviors of $Sm^3+$-ARS complexes has been investigated by d.c.polarography, differential pulse polarography and cyclic voltammetry. $Sm^3+$forms 1 : 3 adsorptive complexes with ARS.The reduction potential of complex wave $(P_2)$ shifted more negatively than the ligand wave $(P_1)$. The linear calibration curves of decreasing $P_1$ and increasing $P_2$ is obtained when $Sm^3+$ concentration varies from TEX>$2{\times}10^{-6}$ M to $3.2{\times}10^{-5}$ M.

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