Browse > Article
http://dx.doi.org/10.5352/JLS.2003.13.2.191

Transcription level of the ars-1 promoter of Neurospora crassa  

이병욱 (고신대학교 생명과학과)
구상호 (고신대학교 생명과학과)
Publication Information
Journal of Life Science / v.13, no.2, 2003 , pp. 191-196 More about this Journal
Abstract
The ars gene of the Neurospora crassa encodes arylsulfatase and is expressed under sulfur limitation. An ars-1 promoter(Pars) translationally-fused to a lacZ gene was transformed into the N. crassa RLM 35-35, a his-3 inl strain and integrated into the his-S locus by a single crossover homologous recombination. $\beta$-galactosidase specific activity was measured from mycelia grown in sulfur-limited Vogel's medium. Enzyme activity reached its maximum at 14 hour after the shift to derepressing condition. When activity from homokaryon generated by microconidiation was measured, it was 17% a higher than that from heterokaryon.
Keywords
Neurospora crassa; ars-1; arylsulfatase; promoter strength;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Heterologous protein production by filamentous fungi /
[ Jeense,D.J.;D.A.Mackenzie;I.N.Roberts;D.B.Archer ] / Biotechnol. Genet. Eng. Rev.   DOI   ScienceOn
2 assembly version 3 /
[ Whitehead Institute/MIT Center for Genome Research ] / Neurospora Sequencing Project
3 Fusing of the Escherichia colilac genes to the ara promoter; a general technique using bacteriophage Mu-1 insertions /
[ Casadaban,M.J. ] / Proc. Natl. Acad. Sci. USA   DOI   ScienceOn
4 A simple method of obtaining pure microconidia in Neurospora crassa /
[ Pandit,A.;R.Maheshwari ] / Fungal Genet. Newsl.
5 A rapid abd sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding /
[ Bradford,M.M. ] / Anal. Biochem.   DOI   ScienceOn
6 Molecular cloning and regulatory analysis of the arylsulfatase structural gene of Neurospora crassa /
[ Paietta,J.V. ] / Mol. Cell. Biol.
7 Combinatorial metabolic libraries /
[ Stuart,W.D. ] / U.S. Patent #6,268,140
8 Beta-galactosidase gene fusions for analyzing gene expression in Escherichia coli and yeast /
[ Casadaban,M.J.;A.Martinez-Arias;S.K.Shapira;J.Chou ] / Meth. Enzymol.   DOI
9 Quantitative evaluation of Escherichia coli host strains for tolerance to cytosine methylation in plasmid and phage recombinants /
[ Woodcock,D.M.;P.J.Crowther;J.Doherty;S.Jefferson;E.DeCruz;M.Noyer-Weidner;S.S.Smith;M.Z.Michael;M.W.Graham ] / Nucleic Acids Res.   DOI   ScienceOn
10 Neurospora: the organism behind the molecular revolution /
[ Perkins,D.D. ] / Genetics
11 The regulation of aryl sulphatase in Aspergillus nidulans /
[ Apte,B.N.;P.N.Bhavsar;O.Siddiqi ] / J. Mol. Biol.   DOI
12 Genetic control of biochemical reactions in Neurospora /
[ Beadle,G.W.;E.L.Tatum ] / Proc. Natl. Acad. Sci. USA   DOI   ScienceOn
13 Expression of a plant protein by Neurospora crassa /
[ Rasmussen-Wilson,S.J.;J.S.Palas;V.J.Wolf;C.S.Taft;C.P.Selitrennikioff ] / Appl. Environ. Microbiol.
14 Production of heterologous peptides /
[ Radford,A.;J.H.Parish ] / U.S. Patent #5,834,191
15 Cloning and characterization of the multifunctional his-3 gene of Neurospora crassa /
[ Legerton,T.L.;C.Yanofsky ] / Gene   DOI   ScienceOn
16 Genetic and microbial research techniques for Neurospora crassa /
[ Davis,R.H.;F.J.de Serres ] / Methods. Enzymol.
17 Molecular cloning and characterization of the cys-3 regulatory gene of Neurospora crassa /
[ Paietta,J.V.;R.A.Akins;A.M.Lambowitz;G.A.Marzluf ] / Mol. Cell. Biol.
18 Regulatory sequences involved in the translation of Neurospora crassa mRNA: Kozak sequences and stop codon /
[ Bruchez,J.J.P.;J.Eberle;V.E.A.Russo ] / Fungal. Genet. Newsl.
19 Improved plasmids for gene targeting at the his-3 locus of Neurospora crassa by electroporation /
[ Margolin,B.S.;M.Freitag;E.U.Selker ] / Fungal Genet. Newsl.
20 Aryl sulfatase in ascospores of Neurospora crassa /
[ Burton,E.G.;R.L.Metzenberg ] / J. Bacteriol.
21 /
[ Maniatis,T.;E.F.Fritsch;J.Sambrook ] / Molecular Cloning: A Laboratory Manual
22 Evidence for safety of Neurospora species for academic and commercial uses /
[ Perkins,D.D.;R.H.Davis ] / Appl. Environ. Microbiol.   DOI   ScienceOn
23 Neurospora expression system /
[ Stuart,W.D.;J.M.Ivy;K.Koo ] / U.S. Patent #5,695,965
24 Vectors for construction of translational fusions to beta-galactosidase /
[ Ebbole,D. ] / Fungal Genet. Newsl.
25 Location of arylsulfatase in conidia and young mycelia of Neurospora crassa /
[ Scott,W.A.;R.L.Metzenberg ] / J. Bacteriol.
26 Fifty years ago: the Neurospora revolution /
[ Horowitz,N.H. ] / Genetics
27 Novel mutation causing derepression of several enzymes of sulfur metabolism in Neurospora crassa /
[ Burton,E.G.;R.L.Metzenberg ] / J. Bacteriol.
28 Nuclear phenomena associated with heterothalism and homothalism in the ascomycete Neurospora /
[ Dodge,B.O. ] / J. Agric. Res.
29 A particulate fraction from Neurospora crassa exhibiting aryl sulfatase activity /
[ Scott,W.A.;K.D.Munkres;R.L.Metzenberg ] / Arch. Biochem. Biophys.   DOI   ScienceOn
30 The positive-acting sulfur regulatory protein CY53 of Neurospora crassa: nuclear localization, autogenous control, and regions required for transcriptional activation /
[ Kanaan,M.N.;G.A.Marzluf ] / Mol. Gen. Genet.   DOI   ScienceOn
31 Genetic engineering of filamentous fungi /
[ Timberlake,W.E.;M.A.Marshall ] / Science   DOI
32 Molecular cloning and analysis of the scon-2 negative regulatory gene of Neurospora crassa /
[ Paietta,J.V. ] / Mol. Cell Biol.