• 제목/요약/키워드: arrangement effect

검색결과 995건 처리시간 0.037초

Interaction effects of glycine equivalent and standardized ileal digestible threonine in low protein diets for broiler grower chickens

  • Paschal Chukwudi Aguihe;Amanda Barroso Castelani;Camilo Ivan Ospina-Rojas;Eustace Ayemere Iyayi;Paulo Cesar Pozza;Alice Eiko Murakami
    • Animal Bioscience
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    • 제37권6호
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    • pp.1053-1064
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    • 2024
  • Objective: This study aims to investigate the interactive effect of a glycine equivalent (Glyequi) and standardized ileal digestible threonine (SID Thr) levels in low crude protein diets on performance, blood biochemistry, pectoral muscular creatine content and oxidative stability of meat in broiler chickens from 21 to 42 days. Methods: A total of 1,500, twenty-one-day-old Cobb-Vantress male broiler chickens were distributed in a completely randomized 5×3 factorial arrangement of Glyequi×SID Thr with five replicates of 20 birds each. Fifteen dietary treatments of 16.5% CP were formulated to contain five levels of total Glyequi (1.16%, 1.26%, 1.36%, 1.46%, and 1.56%) and three levels of SID Thr (0.58%; 0.68% and 0.78%). Results: Interaction effects (p<0.05) of Glyequi and SID Thr levels were observed for weight gain, carcass yield, pectoral muscular creatine content and serum uric acid. Higher levels of Glyequi increased (p = 0.040) weight gain in 0.58% and 0.68% SID Thr diets compare to the 0.78% SID Thr diet. The SID Thr level at 0.68% improved (p = 0.040) feed conversion compared to other SID Thr diets. Levels of Glyequi equal to or above 1.26% in diets with 0.78% SID Thr resulted in birds with higher (p = 0.033) pectoral muscular creatine content. The breast meat yield observed in the 0.68% SID Thr diet was higher (p = 0.05) compared to the 0.58% SID Thr diet. There was a quadratic effect of Glyequi levels for pectoral pectoral muscular creatine content (p = 0.008), breast meat yield (p = 0.030), and serum total protein concentrations (p = 0.040), and the optimal levels were estimated to be 1.47%, 1.35%, and 1.40% Glyequi, respectively. The lowest (p = 0.050) concentration of malondialdehyde in the breast meat was found in 0.68% SID Thr diets at 1.36% Glyequi. Conclusion: The minimum dietary level of Glyequi needed to improve performance in low crude protein diets is 1.26% with adequate SID Thr levels for broiler chickens.

Effects of yeast and dried kratom leaves (Mitragyna speciosa [Korth] Havil.) supplementation on digestibility, rumen fermentation, blood metabolites and nitrogen balance in goats

  • Soklin Va;Chanadol Supapong;Pin Chanjula
    • Animal Bioscience
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    • 제37권2호
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    • pp.228-239
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    • 2024
  • Objective: The objective of the experiment was to study yeast supplementation (yeast, Y) and dried kratom leaves (DKTL) on the digestibility, ruminal fermentation, blood metabolites and nitrogen balance in goats. Methods: Four of 7 to 8 months old male crossbred (50% Thai Native-Anglo Nubian) goats with average liveweight 20±0.13 kg were randomly assigned according to a 2×2 factorial arrangement in a 4×4 Latin square design to receive four diets ad libitum basis. The study investigated the effects of two levels of yeast (Y) supplementation (Y, 0 and 0.5g/kg dry matter [DM]) along with two levels of DKTL supplementation (DKTL, 0 and 4.44g/kg DM). The experimental groups were as follows: T1 = control group with 0Y+0DKTL, T2 = 0Y+4.44 DKTL, T3 = 0.5Y+0DKTL, and T4 = 0.5Y+4.44 DKTL. Results: The results showed that there were no interactions between Y levels and DKTL levels with respect to total DM intake, but there were significant effects (p<0.05) by levels of Y; goats receiving 0.05 g/kg DM Y had higher than goats fed 0.0 g/kg DM on average (kg/d). A percentage of body weight (% BW) and grams per kilogram of metallic weight (g/kg w0.75) had no influence on yeast levels and DKTL, but there was a difference (p<0.05) by yeast level Y at 0.5 g/kg DM, being higher compared to the non-supplemented group. Apparent digestibility coefficient of nutrition in the form of (DM, organic matter, crude protein, neutral detergent fiber, and acid detergent fiber) was an increased trend in the Y-level complementary group at 0.5 g/kg DM and DKTL at 4.44 g/kg DM, respectively. Protozoa populations decreased in the group receiving Y levels at 0.5 g/kg DM and DKTL levels at 4.44 g/kg DM when compared to group T1. The acetic acid concentration and methane gas generation decreased (p<0.05) in the group receiving Y levels of 0.5 g/kg DM and DKTL levels of 4.44 g/kg DM, while the amount of propionic acid increased (p<0.05). Conclusion: Effects of feeding combinations of Y and DKTL supplementation on feed showed no interaction effect (Y×DKTL) on feed intake, rumen fermentation, bacterial and fungi population. The effect on protozoal populations was lower in the group that was supplemented with DKTL at 4.44 g/kg DM related to synthetic CH4 was reduced.

소설과 말기 암환자를 통해 본 한국인의 죽음의 의미

  • 전혜원;김분한
    • 호스피스학술지
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    • 제3권2호
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    • pp.34-54
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    • 2003
  • Every one experiences death one day, however no one can knows exactly what it is because people can not experience death until it comes, it is therefore impossible to judge correctly on the phenomenon of the death. On the whole, man experiences indirect death through the mass communications such as TV drama, fiction, magazine etc because those methods can easily access by every one. In addition to this, people usually acquire the negative awareness of death through the dramatic change of story like dying of cancer for dramatic effect by giving scare and fear to the cancers. The purpose of this study is to provide basic information on the spiritual care that enables the facing death patients to accept death as a part of life and divert hope from scare about after death by comparing and analyzing of two aspects of death meaning I.e, Korean fiction and the end stage cancer patients. Additionally, for medical staff to understand the facing death cancer patients by making to aware patients correctly and provide the better quality of care. The study was performed from September 28, 2002 to February, 28 2003. The materials of this study were collected by direct data obtained from observation, interviews, note and diary of end stage of cancer patients and written materials acquired from Korean contemporary fiction. Participants of this study were 4 end stage cancer patients including 2 lung cancer patients, 1 liver cancer patient and 1 esophagus cancer patient. The methodology used in this study was divided into two types; Huberman & Miles methodology was used for fiction to find and categorize subject, and Colaizzi, one of phenomenological methodology was used for end stage cancer patients to find the major meaning, subject and categorization. 1.The death investigated in the fiction, was found as a progress of negative emotion, acceptance and sublimation, life related subjects in the negative emotion were tenacity for life, anxiety, lingering attachment, responsibility, abandonment and death related subjects were shock, isolation, fear, scare and rejection. Acceptance related subjects were acceptance, destiny, secularism, preparation and arrangement, and sublimation related subjects were sublimation through Christian and Buddhism. 2.The death showed in the participants was negative emotion, acceptance and sublimation, life related subjects were repentance, anxiety, responsibility and hopelessness, and death related subjects were dejection, solitude, anger, fear and scare. The acceptance was a type of religious acceptance that admitted instantly by reaching an understanding with the God, and death was accepted as a progress of preparation, arrangement, acceptance and hope. Sublimation related subjects were Christian sublimation and relief or destiny incurred from self-reflective sublimation through communications and thoughts. 3.The death in view of fiction and participants were positively accepted both death and negative emotion, and the study disclosed the fact that death was sublimated dependent on religion. 4.The progress of negative emotion, acceptance and sublimation was disclosed more complicated and various in the real end stage cancer patients and acceptance only found in the patients on the form of religious acceptance, according to the results compared with fiction and real end stage cancer patients. The death showed in the fiction was standardized, gradated and similar progress with psychological status of Kubler-Ross. However, death in the participants was showed complex and various feelings simultaneously, and sometimes they accepted death positively. The sublimation through religion was found in Buddhism and Christian in the fiction and mostly Christian in the participants due to a number of Hospice patients. It was found that negative emotion various types of death was more found in the participants than fiction. It is therefore necessary to study on the response of death in various types. In the participants death was incurred more systematic and variously, we knew that nursing practice focused on experience of participants is required and reality on death is much profound than we analyzed and presented, lots of situations and reactions should be premised because we can not completely rule out the negligence possibility of care mediation of participants. In caring for the facing death patients, we discovered and confirmed again through this study that the spiritual care should be needed as a mediation method.

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Polytetrafluoroethylene membrane 및 millipore filter를 섬유소와 병용사용시 성견 치주조직 재생에 미치는 영향 (THE EFFECTS OF POLYTETRAFLUOROETHYLENE MEMBRANE AND MILLIPORE FILTER COMBINED WITH FIBRIN ON THE REGENERATION OF PERIODONTIUM IN DOGS)

  • 주상돈;박재완;서조영
    • Journal of Periodontal and Implant Science
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    • 제23권3호
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    • pp.577-594
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    • 1993
  • Polytetrafluoroethylene membrane 및 millipore filter를 섬유소와 병용사용하여 성견 치주조직 재생에 미치는 영향에 관해 알아보기 위하여 생후 9개월 이상된 잡종성견7마리에 치은박리소파수술후 PTFE membrane를 삽입한 군을 대조군으로, PTFE membrane에 섬유소를 첨가했는 군을 실험1군으로, 수술시 millipore filter를 봉합에 의해 고정시킨군을 실험2군으로, 섬유소로 millipore filter를 고정시킨 군을 실험3군으로 하였다. 술후 각 1, 2, 4, 8, 12 주에 관류고정과 아울러 희생시켜 악골을 채취하고 통법에 의해 후고정, 탈회, 탈수과정을 거쳐 파라핀으로 포매한 후 $4-6{\mu}m$두께의 협, 설측 박편을 제작한 후 H & E 및 Masson’s trichrome염색 후 광학현미경으로 치유결과를 조직학적으로 비교 관찰하여 다음과 같은 결과를 얻었다. 1 주때 대조군 및 실험각군 모두 치은상피조직의 근단이동이 억제된 양상을 나타내었으며, millipore filter를 삽입한 군에서는 섬유소보다 봉합에 의해 막을 고정시킨 경우 술후 1주때 부터 치은퇴축으로 인한 막의 노출이 관찰되었고 술후 4주때 막의 내, 외면에 긴접합상피조직의 근단이동을 관찰할 수 있었으며, PTFE membrane 경우는 섬유소유무에 따른 차이는 관찰되지 않았다. 대조군 및 실험각군 모두 술후 1주때 골양조직을 관찰할 수 있었고 4주때 성숙된 치조골 양상을 보였으며 특히 PTFE membrane인 경우 증가된 협설 측 폭경을 관찰할 수 있었으며, 술후 12주때 실험각군 모두 치조골이 더욱 성숙된 양상을 관찰할 수 있었다. 술후 4주때 대조군, 실험각군 모두 활택된 치근면상에 미약한 신생백악질 및 불규칙적인 교원섬유의 배열을 관찰할 수 있었고 8주때 뚜렷한 백악질의 증가와 12주때에는 신생백악질과 신생치조골 사이에 기능적으로 배열된 교원 섬유를 관찰할 수 있었다. 결론적으로, 섬유소로 막을 고정시킬 경우 치근면과의 긴밀한 접촉을 유지시킬수 있으며, 차단막을 근단쪽으로 위치시켜 막의 노출 위험을 감소시킬수 있으며, 특히 PTFE membrane인 경우 봉합대체물로의 연이은 연구가 필요할 것으로 사료된다.

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인공광 및 차광스크린을 이용한 온실의 일일적산 광합성유효광량자속 조절 (Control of Daily Integral PPE by the Artificial Lighting and shading screen In Greenhouse)

  • 이현우
    • 생물환경조절학회지
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    • 제12권1호
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    • pp.45-53
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    • 2003
  • 본 연구의 목적은 차광스크린 및 인공광을 이용하여 온실에 적절한 일일적산 광합성유효광량자속을 공급하는 기술을 개발하는 것이다. 차광율이 55%와 85%인 두 가지 종류의 차광스크린을 이용하여 목표광량을 얻는데 필요한 차광시간대를 분석한 결과, 조절하고자 하는 목표광량의 크기에 따라 적절한 차광율을 가진 차광재를 선택할 필요가 있는 것으로 판단되었다. 기상조건에 따라 광량변화가 많은 7월과 8월에 대하여 55%의 차광재를 이용하여 광조절 실험을 실시한 결과 무차광시의 실측값과 계산값의 차이가 최대 5 mol$.$ $m^{-2}$ $.$$d^{-1}$ 정도로 나타났다. 이러한 차이는 기상조건에 따라 일일적산 광량이 큰 차이가 있음을 감안하면 대체로 만족할 만한 결과로 판단된다. 광분포 시뮬레이션을 이용하여 적절한 광배치를 찾을 수 있었기 때문에 실제 인공광을 배치하는데 소요되는 시간을 많이 단축할 수 있었지만, 정확하게 목표한 광강도를 고르게 분포하도록 할 수 있는 광배치를 찾기가 쉽지 않았으며, 이에 대한 보다 더 많은 분석이 요구되었다. 목표한 일일적산 광합성유효광량자속을 얻는데 필요한 보광강도별 보광시간을 기상조건 및 월별로 계산하여 분석한 결과 보광강도는 필요보광량의 크기에 따라 적절한 값을 선택하여야 할 것으로 판단되었다. 55%차광재 및 300$\mu$mol$.$ $m^{-2}$ $.$ $s^{-1}$의 보광강도를 이용하여 5월말에서 6월초까지 광량 제어실험을 실시한 결과 최대 3mol$.$ $m^{-2}$ $.$$d^{-l}$ 정도의 오차를 보여주었다. 이러한 차이는 동일한 달에 같은 기상조건에서도 일일적산 광량의 차이가 있고 하루 중 기상상태도 많은 변화가 있기 때문인 것으로 판단된다. 그러나 기상조건에 따른 일별 최대차이가 30 mol$.$ $m^{-2}$ $.$$d^{-l}$ 정도임을 감안하면 조절효과는 만족할 만한 결과로 판단된다. 본 연구의 결과는 비록 상추재배를 위한 광량조절기술이지만 다른 작물에 대해서도 본 연구에서 제시한 자료 및 조절방법을 동일하게 적용할 수 있을 것으로 기대된다.

Effects of Expander Operating Conditions on Nutrient Digestibility in Finishing Pigs

  • S.L., Traylor;K.C., Behnke;J.D., Hancock;R.H., Hines;S.L., Johnston;B.J., Chae;In K., Han
    • Asian-Australasian Journal of Animal Sciences
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    • 제12권3호
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    • pp.400-410
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    • 1999
  • Five experiments were conducted using finishing pigs (PIC L326 sires $\times$ C15 dams) to examine the effects of expander operating conditions on nutrient digestibility in finishing pigs. The effects of different expanding conditions (0, 11.7, 24.4, $32.5kg/cm^2$) for corn-SBM based diets (Exp. 1), wheat meddlings diet (Exp. 2), sorghum-SBM based diets (Exp. 3) and wheat-SBM based diet (Exp. 4). Exp. 5 was conducted as a $2{\times}4$ factorial arrangement and factors examined were 2 soy products (raw soybean and SBM) and 4 expanding conditions (0, 14.1, 28.1, $42.2kg/cm^2$). In experiment 1, total production rates (p>0.10) were similar among treatments. The amount of fines decreased (cubic effect, p<0.001) as cone pressure was increased from 0 to $11.7kg/cm^2$, with smaller differences as cone pressure was further increased to $35.2kg/cm^2$. Nutrient digestibilities increased (p<0.02) as the feed was subjected to higher cone pressures. Digestibilities of DM, N, and GE were maximized at $24.4kg/cm^2$ cone pressure. The DE of the diet expanded at 24.4 and $35.2kg/cm^2$ increased by 172 and 109 kcal/kg, respectively, compared to the diet processed at $0kg/cm^2$ cone pressure. In experiment 2, total production and screened pellets production rates were similar among the processing treatments (p>0.21). The amount of fines decreased (quadratic effect, p<0.03) by 9 kg/h as cone pressure was increased from 0 to $11.7kg/cm^2$. Digestibilities of DM (p<0.02), N (p<0.001), and GE (p<0.002) were increased as cone pressure was increased from 0 to $35.2kg/cm^2$. DM, N, and GE digestibility in the pigs fed the midds-based diet increased by 8, 13, and 10%, respectively, at the highest processing cone pressure compared to the diets without any cone pressure. In experiment 3, the conditioned mash moistures for the treatments were numerically similar around 15% moisture. As the expander cone pressure was increased from 0 to $11.7kg/cm^2$, energy consumption for the pellet mill decreased (quadratic effect, p<0.004) from 14.1 to 12.0 kWh/t. Dry matter and gross energy digestibility increased (cubic effects, p<0.006) as cone pressure was increased from 0 to $35.2kg/cm^2$ with the largest improvement occurring as cone pressure was increased from 0 to $11.7kg/cm^2$. Nitrogen digestibility increased (cubic effect, p<0.001) from 78.3 to 81.0% as the feed was subjected to the higher cone pressures, with N digestibility being maximized at $24.4kg/cm^2$ cone pressure. The DE of the diet increased (cubic effect, p<0.001) by 225 kcal/kg as cone pressure was increased from 0 to $11.7kg/cm^2$. In experiment 4, pellet moisture decreased and moisture loss increased as cone pressure was increased from 0 to $35.2kg/cm^2$. Also, starch gelatinization of the wheat-based diets increased from 16.8 to 49.1% as the diets were processed at 0 and $35.2kg/cm^2$ cone pressure. Nutrient digestibilities were not affected (p>0.18) by any increase in cone pressure. In experiment 5, pellet moisture decreased as cone pressure was increased 0 to $35.2kg/cm^2$. The amount of moisture loss for the diets expanded at $42.2kg/cm^2$ was 3.0 and 3.8% for the SBM and raw soybean (RB) diets, respectively. Starch gelatinization for the SBM diets were 19% greater than the RB diets. The RB diets had lower DM, N and GE digestibilities as compared to the SBM diets. The DE of the RB diets were lower (p<0.02) than the SBM diets. DM (p<0.06), N (p<0.02), and GE (p<0.001) digestibilities of the dietary treatments increased as cone pressure was increased 0 to $42.2kg/cm^2$.

CFD 모델을 이용한 도시 재정비 사업에 의한 NOX 분포 변화 모의 (CFD Simulation of Changesin NOX Distribution according to an Urban Renewal Project)

  • 김지현;김연욱;도현석;곽경환
    • 환경영향평가
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    • 제30권3호
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    • pp.141-154
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    • 2021
  • 본 연구에서는 전산유체역학(CFD) 모델을 이용한 수치 모의에서 춘천시 약사지구 도시 재정비 사업에 의한 약사천 복원과 아파트 단지 건설이 주변 지역의 오염물질 농도에 미치는 영향을 분석하였다. 사업에 의한 영향을 비교하기 위해 도시재정비 사업 전과 후인 2011년과 2017년의 지형 자료를 이용하여 바람장과 오염물질 농도장을 모의하였다. 수치 실험에서 아파트 단지 건설의 영향과 하천 복원의 효과를 구분하여 분석하도록 시나리오를 구성하였다. 대상 지역의 평균적인 배경 바람장을 반영하기 위해 춘천 종관기상관측소(ASOS)의 풍향 및 풍속 자료를 유입 경계 조건으로 사용하고, 모의 결과를 유입 풍향의 8방위별 빈도에 따라 가중평균하였다. 시나리오 간 건물·지형 변화에 따른 풍속과 NOX 농도 분포의 차이를 비교하였다. 그 결과 주변 도로에서 배출된 NOX 농도는 아파트 단지 건설에 의해 증가하였으며, 아파트 단지 건설과 하천 복원을 함께 고려한 결과에서는 증가 폭이 감소하였다. 이를 지점별로 나누어볼 때, 복원한 하천 주변으로는 NOX 농도가 감소하는 한편, 건설한 아파트 단지 주변으로는 농도가 크게 증가하였다. 아파트 단지 주변의 NOX 농도 증가는 풍향을 기준으로 아파트 단지의 후면에 위치한 곳에서 더욱 뚜렷하였으며, 그 영향은 건물 높이까지 나타났다. 이러한 결과를 통해 사업 대상 지역의 주풍향에 대한 아파트 단지 건설과 하천 복원의 상대적인 배치가 주변 대기질을 결정하는 주요 요소임을 확인하였다.

Streptozotocin-유발 당뇨쥐에 대한 클로렐라 열수 추출물의 혈당 강하 효과 (Hypoglycemic Effect of Chlorella sp. CMS-1 Hot Water Extract on Streptozotocin-Induced Diabetic Rats)

  • 김정욱;차재영;허진선;진현진;조영수
    • 생명과학회지
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    • 제18권11호
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    • pp.1584-1591
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    • 2008
  • 클로렐라의 생리활성작용 중 인슐린작용 활성 및 항당뇨효능이 보고된 바 있으나 클로렐라 열수 추출물(Chlorella hot water extract, CE)에 의한 혈당치 조절에 관한 연구는 보고된 바 없다. 따라서 본 실험에서는 실험적인 당뇨유발물질 streptozotocin (STZ)을 성장기 SD계 수컷 흰쥐에 단회 복강 주사하여 당뇨를 유발시킨 CE 무첨가 STZ-Control군, CE 3% 첨가 STZ+CE3군 및 CE 6% 첨가 STZ+CE6군, 그리고 정상군(Normal군)으로 실험 설계하여 4주간 식이와 함께 물을 자유 급여하였다. 공복 및 비공복시 혈당치는 정상군보다 당뇨 대조군에서 현저히 증가하여 당뇨 유발이 확인되었고, 이러한 혈당치 증가는 클로렐라 열수 추출물 분말 3% 첨가 STZ+CE3군 및 6% 첨가 STZ+CE6군에서 각각 감소하였다. 혈중 인슐린 농도는 당뇨 대조군에서 낮게 나타났으나 클로렐라 열수 추출물 분말 첨가식이 투여군들에서 인슐린 농도 감소현상이 상쇄되었다. 또한 정상군보다 STZ- 당뇨 대조군에서 Fructosamine 및 뇨당이 증가하였으나, 클로렐라 추출물 투여 STZ+CE3군 및 STZ+CE6군에서 감소하는 경향을 나타내었고, 특히 이러한 효과는 클로렐라 열수 추출물 첨가농도 의존적이었다. 클로렐라 추출물 투여군에서 혈당치 감소의 기작을 검토하기 위하여 인슐린 합성과 분비에 관여하는 췌장의 췌도 베타세포의 면역반응에 의한 조직검사에서 정상군의 정상적인 둥근 모양의 형태를 잘 유지하고 있었으나, STZ-당뇨 대조군에서는 베타세포가 심하게 파괴되어 극소수 존재함으로서 면역 반응성이 매우 미약하였다. 그러나 클로렐라 열수 추출물 투여군에서 농도 의존적으로 베타세포가 많아지면서 정상군과 비슷한 췌도 세포의 형태를 유지하고 있었다. 클로렐라 열수 추출물은 STZ 에 의해 파괴된 베타세포를 회복시키는 효능이 강한 것으로 보여 진다. 이러한 결과는 클로렐라 열수 추출물에 의해 인슐린을 생산해 내는 췌도 베타세포가 회복됨에 따라 인슐린 증강 작용으로 혈당치 강하 효과가 실험 동물을 통하여 확인되었다.

사료의 에너지 및 조단백질 함량이 토종오리의 성장과 도체 특성에 미치는 영향 (Effect of Dietary Metabolizable Energy and Crude Protein Concentrations on Growth Performance and Carcass Characteristics of Korean Native Ducks)

  • 김홍래;권형주;오성택;윤정근;최영인;추연경;강보석;김학규;홍의철;강창원;안병기
    • 한국가금학회지
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    • 제39권3호
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    • pp.167-175
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    • 2012
  • 본 실험은 대사 에너지와 조단백질 수준이 토종오리의 생산성 및 도체 특성에 미치는 영향을 연구하기 위해 수행되었다. 육성 전기는 토종오리 828수를 9개 처리에 공시하여 사용하였고, 육성 후기는 육성 전기를 거친 토종오리 중 720수를 9개 처리에 공시하여 사용하였다. 육성 전기의 실험 사료는 ME 3,000, 2,900 및 2,800 kcal/kg, CP 23, 22 및 21%이었으며, 육성 후기에는 ME 3,100, 3,000 및 2,900 kcal/kg, CP 19, 18 및 17%로 처리하여 사용하였다. 육성 전기 실험 결과는 CP 수준이 증가함에 따라 체중, 증체량 및 사료 요구율이 유의적으로 개선되었지만(Linear effect, P<0.01), ME 수준과 $ME{\times}CP$ interaction에 의한 유의차는 나타나지 않았다. 균일도는 ME, CP 수준과 $ME{\times}CP$ interaction 모두에서 유의차가 나타나지 않았다. 육성 후기 실험 결과는 체중, 섭취량, 증체량, 사료 요구율 및 균일도에서 유의한 차이가 관찰되지 않았으나, 도체율에서는 ME 3,000 kcal/kg에서 유의적으로 가장 높게 나타났으며(Quadratic effect, P<0.01), ME와 CP의 수준이 증가함에 따라 간의 상대적 중량이 유의적으로 각각 작아지는 결과(Linear effect, P<0.01)가 나타났다. 가슴육, 넓적다리 및 북채의 상대적 중량에서는 수준간 유의적인 차이는 나타나지 않았다. 토종오리 고기의 화학 및 물리적 특성인 육색, pH, 가열 감량 및 전단력가에서는 ME 및 CP 수준과 $ME{\times}CP$ interaction 모두에서 유의차가 나타나지 않았다.

Effects of Acanthopanax senticosus Polysaccharide Supplementation on Growth Performance, Immunity, Blood Parameters and Expression of Pro-inflammatory Cytokines Genes in Challenged Weaned Piglets

  • Han, Jie;Bian, Lianquan;Liu, Xianjun;Zhang, Fei;Zhang, Yiran;Yu, Ning
    • Asian-Australasian Journal of Animal Sciences
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    • 제27권7호
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    • pp.1035-1043
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    • 2014
  • To investigate the effect of dietary Acanthopanax senticosus polysaccharide (ASPS) on growth performance, immunity, blood parameters and mRNA expression of pro-inflammatory cytokines in immunologically challenged piglets, an experiment employing $2{\times}2$ factorial arrangement concerning dietary ASPS treatment (0 or 800 mg/kg) and immunological challenge (lipopolysaccharide [LPS] or saline injection) was conducted with 64 crossbred piglets (weaned at 28 d of age, average initial body weight of $7.25{\pm}0.21kg$) assigned to two dietary ASPS treatments with 8 replicates of 4 pigs each. Half of the piglets of per dietary treatment were injected with LPS or saline on d 14. Blood samples were obtained at 3 h after immunological injection on d 14 and piglets were slaughtered to obtain spleen samples on d 21. Dietary ASPS did not affect average daily gain (ADG) (p = 0.634), average daily feed intake (ADFI) (p = 0.655), and gain:feed (p = 0.814) prior to LPS challenge. After LPS challenge, for LPS-challenged pigs those fed ASPS had higher ADG and ADFI than the non-supplemented group (p<0.05), and an interaction between $LPS{\times}ASPS$ was observed on the two indices (p<0.05). Dietary ASPS improved lymphocyte proliferation among saline-injected and LPS-injected pigs (p<0.05). Interaction between $LPS{\times}ASPS$ was also revealed on lymphocyte proliferation (p<0.05). Circulatory concentration of IgG was influenced neither by ASPS (p = 0.803) or LPS (p = 0.692), nor their interaction (p = 0.289). Plasma concentration and spleen mRNA expression of interleukin-1beta (IL-$1{\beta}$), interleukin-6 (IL-6), and tumor necrosis factor (TNF)-${\alpha}$ were induced to increase (p<0.05) by LPS challenge, in contrast, these indices were decreased by dietary ASPS (p<0.05), and interactions were found on these cytokines (p<0.05). For LPS-challenged pigs, dietary ASPS also reduced the circulating concentration and spleen mRNA expression of IL-$1{\beta}$, IL-6 as well as TNF-${\alpha}$ (p<0.05). The interaction between $LPS{\times}ASPS$ was also observed on the circulating concentration of insulin-like growth factor-I, ${\alpha}$-acid glycoprotein (${\alpha}$-AGP), nonesterified fatty acid, and glucose (p<0.05). The results of this study demonstrate that dietary ASPS can modulate the release of pro-inflammatory cytokines during immunological challenge, which might enable piglets to achieve better growth performance.