• Journal of Microbiology and Biotechnology
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• v.31 no.2
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• pp.327-337
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• 2021

Fibrinolytic enzymes with a direct mechanism of action and safer properties are currently requested for thrombolytic therapy. This paper reports on a new enzyme capable of degrading blood clots directly without impairing blood coagulation. This enzyme is also non-cytotoxic and constitutes an alternative to other thrombolytic enzymes known to cause undesired side effects. Twenty-four Bacillus isolates were screened for production of fibrinolytic enzymes using a fibrin agar plate. Based on produced activity, isolate S127e was selected and identified as B. subtilis using the 16S rDNA gene sequence. This strain is of biotechnological interest for producing high fibrinolytic yield and consequently has potential in the industrial field. The purified fibrinolytic enzyme has a molecular mass of 27.3 kDa, a predicted pI of 6.6, and a maximal affinity for Ala-Ala-Pro-Phe. This enzyme was almost completely inhibited by chymostatin with optimal activity at 48℃ and pH 7. Specific subtilisin features were found in the gene sequence, indicating that this enzyme belongs to the BPN group of the S8 subtilisin family and was assigned as AprE127. This subtilisin increased thromboplastin time by 3.7% (37.6 to 39 s) and prothrombin time by 3.2% (12.6 to 13 s), both within normal ranges. In a whole blood euglobulin assay, this enzyme did not impair coagulation but reduced lysis time significantly. Moreover, in an in vitro assay, AprE127 completely dissolved a thrombus of about 1 cc within 50 min and, in vivo, reduced a thrombus prompted in a rat tail by 11.4% in 24 h compared to non-treated animals.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.4
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• pp.451-459
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• 1995

In order to clarify the growth characteristic and Brix degree at different internodes, four varieties that are M81E 71-1, a Rio, a Sart and a Tamurama were seeded on, Apr. 5, Apr. 15, Apr. 25, May 5 and May 15, respectively. Delayed seeding increased plant hight and the growth pattern of the stem diameter and weight was tapesed off and lighted to upper location. Brix degree of internode was rapidly increased from just after heading date, and was slowly increased by rising upper side till before heading date. Brix degree of middle location(6th internode) was the highest of the stem in after heading date and interior side was the lowest. Brix percentage of 40∼45 days after heading had more than 15% in case of being seeded from Apr. 5 to May 5. Sweet sorghum that was seeded on May 15 was more than 17% in the Brix percentage of 35 days after heading and so the suitable seeding date in sourthern district was suggested on May 5. The plethora of soil moisture by rainfall was made decently lower Brix degree in 130∼140 days after seeding. Among the length and the diameter of internode, and Brix degree had negative correlation, between diameter and weight of internode were significantly positive correlation. But correlation coefficient had been a little bit different by varieties.

• Journal of Microbiology and Biotechnology
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• v.30 no.11
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• pp.1720-1728
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• 2020

We have previously characterized AprESJ4, the major fibrinolytic enzyme from Bacillus subtilis SJ4 (Yao et al., 2019). During that study, we observed a 68 kDa protein with fibrinolytic activity. In this study, we cloned the gene (vprSJ4) encoding the 68 kDa protein, a mature Vpr and minor protease secreted by Bacillus species. vprSJ4 encodes a preproenzyme consisting of 810 amino acids (aa) including signal sequence (28 aa) and prosequence (132 aa). The mature enzyme (650 aa) has a predicted molecular weight of 68,467.35. Unlike Vprs from other B. subtilis strains, VprSJ4 has 4 additional amino acids (DEFA) at the C-terminus. vprSJ4 was overexpressed in Escherichia coli. PreproVprSJ4 was localized in inclusion bodies, and subjected to in vitro renaturation and purification by an affinity column. SDS-PAGE and western blot showed that autoprocessing of preproVprSJ4 occurred and 68 kDa and smaller proteins were produced. The optimum pH and temperature of the recombinant VprSJ4 were pH 7.0 and 40℃, respectively. Kinetic parameters of recombinant VprSJ4 were measured by using an artificial substrate, N-succinyl-ala-ala-pro-phe-p-nitroanilide. Coexpression of vprSJ4 and aprESJ4 using pHY300PLK increased the fibrinolytic activity a further 117% when compared with aprESJ4 single expression using the same vector in B. subtilis WB600.

• Journal of Korean Theatre Studies Association
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• no.40
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• pp.239-275
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• 2010

Cette ${\acute{e}}tude$ a pour but de proposer une $m{\acute{e}}thodologie$ de critique avec la $s{\acute{e}}miotique$ ouverte. La critique de $th{\acute{e}}{\hat{a}}tre$ commence ${\grave{a}}$ lire le $th{\acute{e}}{\hat{a}}tre$, l'analyse et juge son valeur. Il arrive souvent qu'on juge avec intutition. On dit que c'est une critique d'impressionnisme. Cette critique est subjective, mais pas objective. La $s{\acute{e}}miotique$ de Saussure offre la $m{\acute{e}}thodologie$ scientifique ${\grave{a}}$ la critique. A $c{\hat{o}}t{\acute{e}}$ de la critique d'impressionnisme qui est subjective, la $s{\acute{e}}miotique$ peut expliciter la raison objective. On ${\acute{e}}tait$ admiratif devant sa scientisme, pourtant $apr{\grave{e}}s$ quoi on critique sa non-$subjectivit{\acute{e}}$ et sa non-$historicit{\acute{e}}$. Dans l'opposition de $l^{\prime}objectivit{\acute{e}}$ et de la $subjectivit{\acute{e}}$, on tente de rechercher un model $int{\acute{e}}gr{\acute{e}}$ dialectiquement entre l'impressionisme(subjective) et la scientisme(objective). Pour cela, on doit aux Ecrits de linguistique $g{\acute{e}}n{\acute{e}}rale$ ($publi{\acute{e}}$ en 2002 chez Gallimard). Ces Ecrits nous aident ${\grave{a}}$ amener la $s{\acute{e}}miotique$ $ferm{\acute{e}}e$ sur la $s{\acute{e}}miotique$ ouverte et ${\grave{a}}$ $red{\acute{e}}couvrir$ la $pens{\acute{e}}e$ de Saussure. Ils nous font ouvrir un nouveau champ de recherche pour la $s{\acute{e}}miotique$ ouverte. L'essentiel de la $th{\acute{e}}orie$ saussurienne du signe $d{\acute{e}}pend$ de l'arbitraire et du circulaire du signe. On $red{\acute{e}}couvre$ la notion ${\acute{e}}largie$ du signe, dans Ecrits de linguistique $g{\acute{e}}n{\acute{e}}rale$, contre le courant majeur de linguistique et de structuralisme. Cette notion s'y focalise, ${\grave{a}}$ la valeur, ${\grave{a}}$ la $relativit{\acute{e}}$, ${\grave{a}}$ la $diff{\acute{e}}rence$ et au $syst{\grave{e}}me$. Avec elle, on tente d'adopter la $s{\acute{e}}miotique$ ouverte pour rechercher une $m{\acute{e}}thodologie$ de critique qui se veut objective et ${\grave{a}}$ la fois subjective. Il s'agit d'une difficile combinaison de l'impressionisme et de la scienticisme. Pour cela, la $m{\acute{e}}thodologie$ se $d{\acute{e}}veloppera$ en trois ${\acute{e}}tapes$. $1{\grave{e}}re$ ${\acute{e}}tape$: c'est lire le $th{\acute{e}}{\hat{a}}tre$ comme un signe total pour 1er jugement d'impressionnisme. $2{\grave{e}}me$ ${\acute{e}}tape$: c'est retrouver sa structure invisible dans la $relativit{\acute{e}}$ des signes. $3{\grave{e}}me$ ${\acute{e}}tape$: c'est juger, dans leur $relativit{\acute{e}}$, comment les $d{\acute{e}}tails$ de signes se fonctionnent. C'est lire les $d{\acute{e}}tails$ de signes et puis $r{\acute{e}}affirmer$ le jugement en $1{\grave{e}}re$ ${\acute{e}}tape$. Selon les $derni{\grave{e}}res$ deux ${\acute{e}}tapes$, on pourra comparer le premier jugement (impressif) et le dernier jugement (objectif), et enfin s'assumer comme critique. Selon la $m{\acute{e}}thodologie$ $propos{\acute{e}}e$, on pratique la critique sur ${\acute{e}}crit$ par Jean Genet, et mise en $sc{\grave{e}}ne$ par Lee Youn-Taek et par Park Jung-Hee. Pour la critique intertextuelle, on la fera en comparant les deux spectacles avec la $pi{\grave{e}}ce$ de Jean Genet. $D^{\prime}apr{\grave{e}}s$ la comparaison, Lee Youn-Taek met en $sc{\grave{e}}ne$ avec $fid{\acute{e}}lit{\acute{e}}$ la structre et les signes de $d{\acute{e}}tail$ de l'auteur, Park Jung-Hee change sa structre et ses signes pour mettre en $sc{\grave{e}}ne$ la $pi{\grave{e}}ce$ de Genet. Ils se $diff{\grave{e}}rent$ l'un et l'autre: Lee incite le discours de la classe sociale dans le spectacle, et Park y incite le discours du $d{\acute{e}}sir$. La $diff{\acute{e}}rence$ des signes dans la $relativit{\acute{e}}$ apporte la $diff{\acute{e}}rence$ de la signification de discours $th{\acute{e}}{\hat{a}}tral$, et enfin se font changer les significations de deux spectacles.

• The Research Journal of the Costume Culture
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• v.17 no.3
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• pp.407-423
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• 2009

The purpose of this study is to analyze Bakst's stage costume illustrations for Ballets Russes. The Russian Ballets has renown in the West since the early 20th century. Diaghilev first made his name as the organizer of art exhibitions in Russia and Western Europe. Diaghilev risked presenting only ballets in 1909, that time Bakst designed $Sch{\acute{e}}h{\acute{e}}razade$, L'Oiseau de Feu and Le Carnival. $Sch{\acute{e}}h{\acute{e}}razade$ was something new which overwhelmed the French people by then. It was seen to be the achievement of Wagner's idea where all the elements; the music, the design, the choreography, and the dancing fused into a perfect whole. The entire production was a hitherto unseen harmony of colors, sounds and movements. For the 1911 season in Paris, Diaghilev planned to present six new ballets and Bakst designed four ballets. Bakst's design of the set and the costume greatly shocked the audience. His "Originality" shines on the fact that he introduced a palette of colors in theater for the first time in history. He used a magic of colors to suggest the secret meaning of what was happening on the stage. The results can be divided into three distinct characteristics. First, oriental designs such as $Cl{\acute{e}}op{\hat{a}}tre$, $Sch{\acute{e}}h{\acute{e}}razade$, L'Oiseau de Feu, La $P{\acute{e}}ri$ and Le Dieu Blue with Russian, Indian and Persian traditional design in headdress and accessories ars found. Second, Le Carnival and La Spectre de la Rose used romantic styles with oriental details. Third, Greek chiton and Himation are used in Narcisse, $H{\acute{e}}l{\grave{e}}ne$ de Sparte, $L'Apr{\grave{e}}s-Midi\;d^{\grave{\;}}un$ Faune and Daphnis et $Chlo{\acute{e}}$ inspirde with various colors and geometic patterns. Bakst's orient of lush colored costumes, head dress, cushions, rugs and hangings became the immediate fashion imitated by Parisian couture houses and interior decorators.

• Nuclear Engineering and Technology
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• v.53 no.4
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• pp.1127-1133
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• 2021

A Spent Fuel Pool (SFP) is designed to store spent fuel assemblies in the pool. And, a SFP cooling and cleanup system cools the SFP coolant through a heat exchanger which exchanges heat with component cooling water. If the cooling system fails or interfacing pipe (e.g., suction or discharge pipe) breaks, the cooling function may be lost, probably leading to fuel damage. In order to prevent such an incident, it is required to properly cool the spent fuel assemblies in the SFP by either recovering the cooling system or injecting water into the SFP. Probabilistic safety assessment (PSA) is a good tool to assess the SFP risk when an initiating event for the SFP occurs. Since PSA has been focused on reactor-side so far, it is required to study on the framework of PSA approach for SFP and identify the key factors in terms of fuel damage frequency (FDF) through a case study. In this study, therefore, a case study of SFP-PSA on the basis of design information of APR-1400 has been conducted quantitatively, and several sensitivity analyses have been conducted to understand the impact of the key factors on FDF.

• Nuclear Engineering and Technology
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• v.53 no.8
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• pp.2696-2707
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• 2021

The purpose of this study is to investigate the efficiency of various structural modeling schemes for evaluating seismic performances and fragility of the reactor containment building (RCB) structure in the advanced power reactor 1400 (APR1400) nuclear power plant (NPP). Four structural modeling schemes, i.e. lumped-mass stick model (LMSM), solid-based finite element model (Solid FEM), multi-layer shell model (MLSM), and beam-truss model (BTM), are developed to simulate the seismic behaviors of the containment structure. A full three-dimensional finite element model (full 3D FEM) is additionally constructed to verify the previous numerical models. A set of input ground motions with response spectra matching to the US NRC 1.60 design spectrum is generated to perform linear and nonlinear time-history analyses. Floor response spectra (FRS) and floor displacements are obtained at the different elevations of the structure since they are critical outputs for evaluating the seismic vulnerability of RCB and secondary components. The results show that the difference in seismic responses between linear and nonlinear analyses gets larger as an earthquake intensity increases. It is observed that the linear analysis underestimates floor displacements while it overestimates floor accelerations. Moreover, a systematic assessment of the capability and efficiency of each structural model is presented thoroughly. MLSM can be an alternative approach to a full 3D FEM, which is complicated in modeling and extremely time-consuming in dynamic analyses. Specifically, BTM is recommended as the optimal model for evaluating the nonlinear seismic performance of NPP structures. Thereafter, linear and nonlinear BTM are employed in a series of time-history analyses to develop fragility curves of RCB for different damage states. It is shown that the linear analysis underestimates the probability of damage of RCB at a given earthquake intensity when compared to the nonlinear analysis. The nonlinear analysis approach is highly suggested for assessing the vulnerability of NPP structures.

• Journal of the Korean Professional Engineers Association
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• v.43 no.2
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• pp.45-52
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• 2010

At the end of last year, Korean nuclear power plants, APR-1400 and a research reactor have been contracted to build plants at United Arab Emirates and Jordan. Since 1959, a historical background of nuclear technologies in Korea is summarized. The safety requirements for Instrumentation and Control (I&C) systems in Nuclear Power Plants (NPP) are discussed. Specific descriptions on the typical safety classification of I&C systems, the definitions of the electrical class 1E and the countermeasures against common caused failures are provided. And summaries of typical I&C systems such as the protection systems, the control systems, the instrumentations, the monitoring systems and a control room in NPP are introduced. Strict requirements on the development of the digital computer systems in nuclear applications are described.

• Journal of Environmental Science International
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• v.8 no.1
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• pp.19-26
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• 1999

The degree of pesticides accumulation in tap water in Taejon from June 1995 to Apr 1996 was measured by Ellman's coupled enzyme assay. Since organic phosphate and carbamate pesticides specifically inhibit the neurotransmitter modulating enzyme acetylcholinesterase(AChE), the enzyme activity can be used as a diagnosis for the pesticides accumulation in water and various samples. During the period of this study, the enzyme activity was changed almost every week. The lowest enzyme activity was 64 % of that of the control reaction and there are several days showing about 100 % enzyme activity. In general, the enzyme activity is higher in summer than other seasons especially early spring times. The pH value of tap water was very close to neutral(pH 7.0) and it seems that the enzyme activity was not affected by the small pH changes. Either boiling of tap water or addition of NaOH solution decomposed the pesticide components. These results show that AChE assay is a convenient, sensitive, and reliable method for detection of pesticides in water samples.

• Korean Journal of Construction Engineering and Management
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• v.20 no.2
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• pp.28-36
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• 2019

The APR1400 (Advanced Power Reactor 1400MW) nuclear power plant is a large-scale national infrastructure facility with a total project cost of 8.6 trillion won and a project period of 10 years or more. The total project area is about 2.17 million square meters and consists of more than 20 buildings and structures. And the total number of drawings required for construction is about 65,000. In order to design such a large facility, it is important to establish a design standard that reflects the design intent and can increase conformity between documents (drawings). To this end, a design bases document (DBD) reflecting the design bases that extracted in regulatory requirements (e.g. 10CFR50, Korean Law, etc.) is created. However, although the design bases are important concepts that are a big framework for the whole design of the nuclear power plant, they are managed in 2-dimensional by the experts in each field fragmentarily. Therefore, in order to improve the usability of building information, we developed BIM(Building Information Model) based 3-dimensional design bases management system. For this purpose, the concept of design bases information layer (DBIL) was introduced. Through the simulation of developed system, design bases attribute and element data extraction for each DBIL was confirmed, and walls, floors, doors, and penetrations with DBIL were successfully extracted.