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THE EFFECT OF PDGF-BB AND IGF-I COMBINATION ON THE HEALING OF ARTIFICIAL PERIAPICAL LESIONS IN BEAGLE DOGS (PDGF-BB와 IGF-I 혼합 투여가 비글견 인공 치근단 병소의 치유에 미치는 영향에 관한 연구)

  • Kim, Mi-Ri;Kim, Min-Kyum;Yoon, Soo-Han
    • Restorative Dentistry and Endodontics
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    • v.25 no.1
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    • pp.1-16
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    • 2000
  • It is difficult to treat the endodontic apical perforation successfully. In this study, we hypothesized that the application of PDGF-BB and IGF-I into periapical perforation site may accelerate periapical healing and lead to bone deposition. And the specificity of osteonectin in periapical healing was investigated. The experiments were performed on the upper and lower 51 premolar teeth of 4 beagle dogs. The pulp chamber of each tooth was opened and the dental plaque was inserted into the canal for developing the periapical lesion for 5 weeks. Then, the roots were artificially perforated at the apex with the number 4 profile of .06 taper. In each step, standard periapical radiographs were taken to compare the size of lesion each other. The radiographs were scanned and analyzed by image analysis system. The mean and standard deviation of periradicular radiolucency ratios were calculated in each group. ANOVA was used for comparison. 51 premolars were grouped into 3 groups; control group, calcium hydroxide-treated group and calcium hydroxide plus growth factors-treated group. In the control group, the apical perforations were not sealed and obturated with gutta-percha and ZOE sealer by lateral condensation technique. In the experimental groups, the apical perforation were sealed with calcium hydroxide and with/without $4{\mu}g$ of PDGF-BB & IGF-I in cellulose gel and obturated by lateral condensation technique. Fluorescent bone markers were used to measure new bone formation. Following 2, 4, 12 weeks after experiment the dogs were sacrificed and histologic sections were prepared. Each tooth block including periapical lesion was sectioned mesiodistally. One half of the sections were decalcified with 6% nitric acid and processed by standard paraffin embedding technique. The sections were stained by hematoxylin and eosin, and immunostained for osteonectin. Histomorphometrical measurement of neoformed bone was performed using a light microscope. And the other half of the sections were prepared by undecalcified preparation, and confocal laser scanning microscopic investigations were done.

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Comparisons of Collection 5 and 6 Aqua MODIS07_L2 air and Dew Temperature Products with Ground-Based Observation Dataset (Collection 5와 Collection 6 Aqua MODIS07_L2 기온과 이슬점온도 산출물간의 비교 및 지상 관측 자료와의 비교)

  • Jang, Keunchang;Kang, Sinkyu;Hong, Suk Young
    • Korean Journal of Remote Sensing
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    • v.30 no.5
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    • pp.571-586
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    • 2014
  • Moderate Resolution Imaging Spectroradiometer (MODIS) provides air temperature (Tair) and dew point temperature (Tdew) profiles at a spatial resolution of 5 km. New Collection 6 (C006) MODIS07_L2 atmospheric profile product has been produced since 2012. The Collection 6 algorithm has several modifications from the previous Collection 5 (C005) algorithm. This study evaluated reliabilities of two alternative datasets of surface-level Tair and Tdew derived from C005 and C006 Aqua MODIS07_L2 (MYD07_L2) products using ground measured temperatures from 77 National Weather Stations (NWS). Saturated and actual vapor pressures were calculated using MYD07_L2 Tair and Tdew. The C006 Tair showed lower mean error (ME, -0.76 K) and root mean square error (RMSE, 3.34 K) than the C005 Tair (ME = -1.89 K, RMSE = 4.06 K). In contrasts, ME and RMSE of C006 Tdew were higher than those (ME = -0.39 K, RMSE = 5.65 K) of C005 product. Application of ambient lapse rate for Tair showed appreciable improvements of estimation accuracy for both of C005 and C006, though this modification slightly increased errors in C006 Tdew. The C006 products provided better estimation of vapor pressure datasets than the C005-derived vapor pressure. Our results indicate that, except for Tdew, C006 MYD07_L2 product showed better reliability for the region of South Korea than the C005 products.

Comparative Study of GDPA and Hough Transformation for Linear Feature Extraction using Space-borne Imagery (위성 영상정보를 이용한 선형 지형지물 추출에서의 GDPA와 Hough 변환 처리결과 비교연구)

  • Lee Kiwon;Ryu Hee-Young;Kwon Byung-Doo
    • Korean Journal of Remote Sensing
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    • v.20 no.4
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    • pp.261-274
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    • 2004
  • The feature extraction using remotely sensed imagery has been recognized one of the important tasks in remote sensing applications. As the high-resolution imagery are widely used to the engineering purposes, need of more accurate feature information also is increasing. Especially, in case of the automatic extraction of linear feature such as road using mid or low-resolution imagery, several techniques was developed and applied in the mean time. But quantitatively comparative analysis of techniques and case studies for high-resolution imagery is rare. In this study, we implemented a computer program to perform and compare GDPA (Gradient Direction Profile Analysis) algorithm and Hough transformation. Also the results of applying two techniques to some images were compared with road centerline layers and boundary layers of digital map and presented. For quantitative comparison, the ranking method using commission error and omission error was used. As results, Hough transform had high accuracy over 20% on the average. As for execution speed, GDPA shows main advantage over Hough transform. But the accuracy was not remarkable difference between GDPA and Hough transform, when the noise removal was app]ied to the result of GDPA. In conclusion, it is expected that GDPA have more advantage than Hough transform in the application side.

Application of Analysis Models on Soil Water Retention Characteristics in Anthropogenic Soil (인위적으로 변경된 토양에서의 수분보유특성 해석 모형의 적용)

  • Hur, Seung-Oh;Jeon, Sang-Ho;Han, Kyung-Hwa;Jo, Hee-Rae;Sonn, Yeon-Kyu;Ha, Sang-Keun;Kim, Jeong-Gyu;Kim, Nam-Won
    • Korean Journal of Soil Science and Fertilizer
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    • v.43 no.6
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    • pp.823-827
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    • 2010
  • This study was conducted to assess the propriety of models for soil water characteristics estimation in anthropogenic soil through the measurement of soil water content and soil water matric potential. Soil profile was characterized with four different soil layers. Soil texture was loamy sand for the first soil layer (from soil surface to 30 cm soil depth), sand for the second (30~70 cm soil depth) and the third soil layers (70~120 cm soil depth), and sandy loam for the fourth soil layer (120 cm < soil depth). Soil water retention curve (SWRC), the relation between soil water content and soil water matric potential, took a similar trend between different layers except the layer of below 120 cm soil depth. The estimation of SWRC and air entry value was better in van Genuchten model by analytical method than in Brooks-Corey model with power function. Therefore, it could be concluded that van Genuchten model is more desirable than Brook-Corey model for estimating soil water characteristics of anthropogenic soil accumulated with saprolite.

Feasibility Study on the Methodology of Test and Evaluation for UAV Positioning (무인항공기 위치정확도 시험평가 기법 연구)

  • Ju, Yo-han;Moon, Kyung-kwan;Kang, Bong-seok;Jeong, Jae-won;Son, Han-gi;Cho, Jeong-hyun
    • Journal of Advanced Navigation Technology
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    • v.22 no.6
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    • pp.530-536
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    • 2018
  • Recently, many studies for interoperability of UAV in the NAS has been performed since the application range and demand of UAV are continuously increased. For the interoperation of UAV in the NAS, technical standards and certification system for UAV which is equivalent to the commercial aircraft are required and test and evaluation methodology must be presented by standards. In this paper, qualification test and evaluation methodology aboutfor the UAV navigation system is proposed. For the research, the mission profile and operation environment of UAV were analyzed. Thereafter the test criteria were derived and the test methodology were established. Finally, the simulation and demonstration using test-bed UAV were performed. As a result of the test, it was confirmed that the navigation system of test UAV has a position accuracy about 1.4 meters at 95% confidence level in the entire flight stage.

A Study of the Diversity and Profile for Extracellular Enzyme Production of Aerobically Cultured Bacteria in the Gut of Muraenesox cinereus (갯장어(Muraenesox cinereus) 장으로부터 호기적 조건에서 분리된 미생물의 다양성 및 세포외 효소 생산능 분석에 관한 연구)

  • Lee, Yong-Jik;Oh, Do-Kyoung;Kim, Hye Won;Nam, Gae-Won;Sohn, Jae Hak;Lee, Han-Seung;Shin, Kee-Sun;Lee, Sang-Jae
    • Journal of Life Science
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    • v.29 no.2
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    • pp.248-255
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    • 2019
  • This research confirmed the diversity and characterization of gut microorganisms isolated from the intestinal organs of Muraenesox cinereus, collected on the Samcheonpo Coast and Seocheon Coast in South Korea. To isolate strains, Marine agar medium was basically used and cultivated at $37^{\circ}C$ and pH7 for several days aerobically. After single colony isolation, totally 49 pure single-colonies were isolated and phylogenetic analysis was carried out based on the result of 16S rRNA gene DNA sequencing, indicating that isolated strains were divided into 3 phyla, 13 families, 15 genera, 34 species and 49 strains. Proteobacteria phylum, the main phyletic group, comprised 83.7% with 8 families, 8 genera and 26 species of Aeromonadaceae, Pseudoalteromonadaceae, Shewanellaceae, Enterobacteriaceae, Morganellaceae, Moraxellaceae, Pseudomonadaceae, and Vibrionaceae. To confirm whether isolated strain can produce industrially useful enzyme or not, amylase, lipase, and protease enzyme assays were performed individually, showing that 39 strains possessed at least one enzyme activity. Especially the Aeromonas sp. strains showed all enzyme activity tested. This result indicated that isolated strains have shown the possibility of the industrial application. Therefore, this study has contributed for securing domestic genetic resources and the expansion of scientific knowledge of the gut microbial community in Muraenesox cinereus of South Korea.

Efficacy of combination of endo-xylanase and xylan-debranching enzymes in improving cereal bran utilization in piglet diet

  • Wang, Weiwei;Zheng, Dawen;Zhang, Zhenzhen;Ye, Hui;Cao, Qingyun;Zhang, Changming;Dong, Zemin;Feng, Dingyuan;Zuo, Jianjun
    • Animal Bioscience
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    • v.35 no.11
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    • pp.1733-1743
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    • 2022
  • Objective: This study was aimed to explore the efficacy of combination of endo-xylanase (Xyn) and xylan-debranching enzymes (arabinofuranosidase, Afd and feruloyl esterase, FE) in improving utilization of bran in piglet diet. Methods: In vitro experiments were firstly conducted to examine the enzymological properties of Xyn, Afd, and FE, concurrent with their effect on degradation of arabinoxylan (Abx) in bran. In vivo experiment was then implemented by allocating two hundred and seventy 35-d-old postweaning piglets into 3 groups (6 replicates/group), which received bran-containing diet supplemented with Xyn (1,600 U/kg) or its combination with Afd (0.8 U/kg) and FE (4 U/kg) or without enzyme. Results: Both Xyn, Afd, and FE are relatively stable against the changes in temperature and pH value. Combining Xyn with Afd and FE had a superiority (p<0.05) over Xyn alone and its combination with Afd or FE in promoting (p<0.05) degradation of Abx in different brans. Combined treatment with Xyn, Afd, and FE was more beneficial than Xyn alone to induce increasing trends (p<0.10) of average daily gain, final body weight and feed efficiency of piglets fed bran-containing diet. Moreover, combination of Xyn, Afd, and FE showed advantages (p<0.05) over Xyn alone in causing reductions (p<0.05) in diarrhea rate and cecal pH value, concurrent with increases (p<0.05) in cecal and colonic acetic acid and total volatile fatty acid concentrations, as well as cecal butyric acid concentration of piglets fed bran-containing diet. Conclusion: Combining Xyn with Afd and FE was more beneficial than Xyn alone in promoting degradation of Abx in bran, along with growth performance and intestinal volatile fatty acid profile of piglets received bran-containing diet. Thereby, combination of Xyn, Afd, and FE had a superior efficacy relative to Xyn alone in improving application of cereal bran in piglet diet.

A Study on Digital Color Reproduction for Recording Color Appearance of Cultural Heritage (문화유산의 현색(顯色) 기록화를 위한 디지털 색재현 연구)

  • Song, Hyeong Rok;Jo, Young Hoon
    • Journal of Conservation Science
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    • v.38 no.2
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    • pp.154-165
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    • 2022
  • The color appearance of cultural heritage are essential factors for manufacturing technique interpretation, conservation treatment usage, and condition monitoring. Therefore, this study systematically established color reproduction procedures based on the digital color management system for the portrait of Gwon Eungsu. Moreover, various application strategies for recording and conserving the cultural heritage were proposed. Overall color reproduction processes were conducted in the following order: photography condition setting, standard color measurements, digital photography, color correction, and color space creation. Therefore, compared with the color appearance, the digital image applied to a camera maker profile indicated an average color difference of 𝜟10.1. However, the digital reproduction result based on the color management system exhibits an average color difference of 𝜟1.1, which is close to the color appearance. This means that although digital photography conditions are optimized, recording the color appearance is difficult when relying on the correction algorithm developed by the camera maker. Therefore, the digital color reproduction of cultural heritage is required through color correction and color space creation based on the raw digital image, which is a crucial process for documenting the color appearance. Additionally, the recording of color appearance through digital color reproduction is important for condition evaluation, conservation treatment, and restoration of cultural heritage. Furthermore, standard data of imaging analysis are available for discoloration monitoring.

A Study of Obtaining Reliable Travel Time Information in Downhole Seismic Method (다운홀 기법에서 신뢰성 있는 도달시간 정보 산출 방법에 대한 고찰)

  • Bang, Eun-Seok;Lee, Sei-Hyun;Kim, Jong-Tae;Kim, Dong-Soo
    • Journal of the Korean Geotechnical Society
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    • v.23 no.8
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    • pp.17-33
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    • 2007
  • Downhole seismic method is widely used for obtaining shear wave velocity profile of a site because it is simple and economical. Determining accurate travel time of shear wave is very important to obtain reliable result in downhole seismic method. In this paper, comparison study of various travel time determination methods was performed. Numerical study and model chamber test were performed for effective comparison study. Signal traces were acquired by performing downhole test at each numerical simulation and soil box test. Travel time data for each signal traces were determined by using six different methods and Vs profiles were evaluated. Comparing travel time data and Vs profiles with the reference value, the first arrival picking method proved to be ambiguous and unreliable. Other methods also did not always provide accurate results and the magnitude of error was dependent on the signal to noise ratio. Cross-correlation method proved to be the most adequate method for the field application and it was verified additionally with field data.

Porosity Evaluation of Offshore Soft Soils by Electrical Resistivity Cone Probe (전기비저항 콘 프로브를 이용한 해안 연악 지반의 간극률 산정)

  • Kim, Joon-Han;Yoon, Hyung-Koo;Choi, Yong-Kyu;Lee, Jong-Sub
    • Journal of the Korean Geotechnical Society
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    • v.25 no.2
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    • pp.45-54
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    • 2009
  • The electrical characteristics of soils have been used for investigating soil properties. The purpose of this study is the development and application of the electrical resistivity cone probe (ERCP) for the evelation of the porosity in the field with high precision. The shape of the probe tip is a cone shape to minimize the disturbance during penetration. In addition, the four terminal pair configuration is adopted to minimize the electrical interference. The electrical resistances are continuously measured during penetration of the ERCP using penetration rigs with 0.33 mm/sec penetration rate at Incheon and Busan sites. With the measured resistance profile and electrical resisivity of electrolyte of undisturbed samples, soil porosity profiles are obtained by using Archie's law. The empirical coefficients for the Archie's law are obtained based on the electrolyte extracted from the undisturbed samples. The estimated porosity profiles show similar trends to those of in-situ penetration tests such as SPT, CPT, and DMT. This study suggests that the ERCP may be an effective tool for the porosity estimation in the field with minimum disturbance.