Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Efficacy of combination of endo-xylanase and xylan-debranching enzymes in improving cereal bran utilization in piglet diet

  • Wang, Weiwei (Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University) ;
  • Zheng, Dawen (AsiaPac Bio-Technology Co. Ltd) ;
  • Zhang, Zhenzhen (AsiaPac Bio-Technology Co. Ltd) ;
  • Ye, Hui (Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University) ;
  • Cao, Qingyun (Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University) ;
  • Zhang, Changming (Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University) ;
  • Dong, Zemin (AsiaPac Bio-Technology Co. Ltd) ;
  • Feng, Dingyuan (Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University) ;
  • Zuo, Jianjun (Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Science, South China Agricultural University)
  • Received : 2021.12.07
  • Accepted : 2022.06.27
  • Published : 2022.11.01
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Abstract

Objective: This study was aimed to explore the efficacy of combination of endo-xylanase (Xyn) and xylan-debranching enzymes (arabinofuranosidase, Afd and feruloyl esterase, FE) in improving utilization of bran in piglet diet. Methods: In vitro experiments were firstly conducted to examine the enzymological properties of Xyn, Afd, and FE, concurrent with their effect on degradation of arabinoxylan (Abx) in bran. In vivo experiment was then implemented by allocating two hundred and seventy 35-d-old postweaning piglets into 3 groups (6 replicates/group), which received bran-containing diet supplemented with Xyn (1,600 U/kg) or its combination with Afd (0.8 U/kg) and FE (4 U/kg) or without enzyme. Results: Both Xyn, Afd, and FE are relatively stable against the changes in temperature and pH value. Combining Xyn with Afd and FE had a superiority (p<0.05) over Xyn alone and its combination with Afd or FE in promoting (p<0.05) degradation of Abx in different brans. Combined treatment with Xyn, Afd, and FE was more beneficial than Xyn alone to induce increasing trends (p<0.10) of average daily gain, final body weight and feed efficiency of piglets fed bran-containing diet. Moreover, combination of Xyn, Afd, and FE showed advantages (p<0.05) over Xyn alone in causing reductions (p<0.05) in diarrhea rate and cecal pH value, concurrent with increases (p<0.05) in cecal and colonic acetic acid and total volatile fatty acid concentrations, as well as cecal butyric acid concentration of piglets fed bran-containing diet. Conclusion: Combining Xyn with Afd and FE was more beneficial than Xyn alone in promoting degradation of Abx in bran, along with growth performance and intestinal volatile fatty acid profile of piglets received bran-containing diet. Thereby, combination of Xyn, Afd, and FE had a superior efficacy relative to Xyn alone in improving application of cereal bran in piglet diet.

Keywords

Acknowledgement

This work was financially supported by the National Natural Science Foundation (No. 31872390) and the Modern Feed Industry Innovation Team Project of Guangdong Province (No.2021KJ115).

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