• Title/Summary/Keyword: aphid

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Relation of Aphicidal Activity with Cuticular Degradation by Beauveria bassiana SFB-205 Supernatant Incorporated with Polyoxyethylene-(3)-Isotridecyl Ether

  • Kim, Jae-Su;Je, Yeon-Ho
    • Journal of Microbiology and Biotechnology
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    • v.20 no.3
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    • pp.506-509
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    • 2010
  • The application of Beauveria bassiana SFB-205 supernatant incorporated with polyoxyethylene-(3)-isotridecyl ether (TDE-3) significantly reduced the population of two species of aphids including cotton aphid, Aphis gossypii, and green peach aphid, Myzus persicae, much higher in cotton aphid, compared with supernatant incorporated with Tween 80, which allows the relationship of aphicidal activity with the degradation of aphid cuticles to be determined. Overall, the degradation of the cuticles induced by the supernatant was more remarkable in conjunction with TDE-3 than Tween 80, and this phenomenon was more observable in cotton aphid through SDS-polyacrylamide gel electrophoresis, revealing high correlation with their aphicidal activities.

Effects of Polyethylene Net Barrier on the Control of Aphid - borne Potato Virus Y in Tobacco (폴리에칠렌 차단망 설치에 의한 연초의 진딧물 전염 PVY 방제효과)

  • Chae, S.Y.;Kim, S.S.;Park, E.K.
    • Journal of the Korean Society of Tobacco Science
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    • v.16 no.1
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    • pp.84-89
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    • 1994
  • Potato virus Y (PVY) is an important viral disease in burley tobacco in Korea and is mostly transmitted by green peach aphid, Myzus penicae, in nature. Effects of barrier nets on the immigration of the aphid population into tobacco fields and on the control of PVY were investigated in 3 tobacco fields with 1.8 m - height polyethylene (PE) nets on their outer - sides in Iseo - Myeon, Wanju - Kun, Cheonbuk. Immigration of aphids to the tobacco yields started at late April and reached maximum at early June. The immigrating aphid population was apparently blocked by the barrier nets to be reduced by 54-73%. PVY severity was also reduced by the barrier nets. However, the control value was variable, ranging 24-67%, which suggests that effect of the PE net barrier on the prevention of aphid-borne virus might be variable depending on the location and slope of the fields.

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Aphicidal Activity of Different Fraction Extracts of Culture Filtrate of Beauveria bassiana Isolate against Aphids (Beauveria bassiana 배양여액 분획추출물의 진딧물 살충활성)

  • Jeong, Ga-Young;Han, Ji-Hee;Kim, Jeong-Jun;Lee, Sang-Yeob
    • Korean Journal of Organic Agriculture
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    • v.25 no.1
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    • pp.113-124
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    • 2017
  • Cotton aphid (Aphis gossypii) and green peach aphid (Myzus persicae) are serious pests damaging various crops including vegetables such as pepper, cucumber, and Chinese cabbage. We conducted a study to control two aphids with secondary metabolite of entomopathogenic fungus Beauveria bassiana. A B. bassiana was already selected as a high virulence isolate against cotton aphid and green peach aphid. The culture filtrate of the isolate showed high pathogenicity against both aphids as 100% mortality against cotton aphid 3 days after treatment and 99% against green peach aphid 5 days after treatment. A different fraction extracts with $CHCl_3$ : MeOH of B. bassiana culture filtrate (30:1, 50:1, 70:1, 90:1, 100:1; v/v) through silica gel column chromatography showed different control effect to aphids. Among them, 50:1 ($CHCl_3$ : MeOH) fraction had highest mortality as 77.3% and 75.4% against A. gossypii and M. persicae, respectively. A mixture of each fraction (1:1) had no synergistic effects because control effect of every mixture was lower than only 50:1 extract; for example, mortality of 50:1 + 70:1 showed $2^{nd}$ highest as 72% of cotton aphid and 70.2% of green peach aphid and other mixtures were lower than these values. In future we will study the identification and mass production of aphicidal compound isolated from 50:1 fraction to develop stable aphid control agent.

Screening of Plant Extracts and Identification of their Insecticidal Metabolites against Myzus persicae (복숭아혹진딧물 방제용 식물추출물 탐색 및 살충성분 구명)

  • Yang, Si young;Lim, Da jung;Kim, Yeo Hee;Kim, In Seon
    • Korean Journal of Environmental Agriculture
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    • v.37 no.2
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    • pp.125-134
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    • 2018
  • BACKGROUND: Green peach aphid (Myzus persicae) is an insect pest that significantly affects crop production. A number of pesticides have been used for aphid control, but their concerns on insect resistance and food safety have required alternative methods for pest management. In an effort to find for an alternative approach to aphid control, we screened plants extracts and examined their potentiality as insecticidal bio-resources. METHODS AND RESULTS: Two hundred and ninety eight plant extracts were examined for insecticidal activity against the aphid, and the best candidate among them was chosen for further study. The extracts from Cinnamomum camphora was determined to be the best candidate exhibiting insecticidal activity more than 60% at a level of $1,000{\mu}g/mL$. GC/MS analyses detected camphor, borneol, 4-terpineol, ${\alpha}$-terpineol and caryophyllene oxide as major compositions from the extracts obtained by hydrodistillation. Caryophyllene oxide exhibited the highest insecticidal activity with a $LC_{50}$ value of $237{\mu}g/mL$. Camphor lowered significantly the $LC_{50}$ value of caryophyllene oxide and increased largely its concentration in aphid, suggesting that camphor played a role in enhancing the insecticidal activity of caryophyllene oxide. CONCLUSION: This study suggested that camphor and caryophyllene oxide may be used as an insecticidal bio-resource for insect control against green peach aphid.

Effect of Aphis gossypii Glover on Growth and Sugar Content of Oriental Melon (목화진딧물(Aphis gossypii Glover)이 참외의 생육과 당함량에 미치는 영향)

  • Do, Han-Woo;Suh, Dong-Whan;Kwon, Min-Kyung;Choi, Sung-Kuk;Shin, Yong-Seub
    • Journal of Bio-Environment Control
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    • v.13 no.1
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    • pp.16-20
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    • 2004
  • Aphis gossypii Glover(Homoptera: Aphididae) is an serious pest on various crops. This experiment was conducted to evaluate the effect of A. gossypii Glover on the growth of oriental melon and the plant recovery after removing A. gossypii. Visible damage symptom caused by A. gossypii feeding to oriental melon was leaf distortion and stunting. A. gossypii feeding for 20 days on oriental melon significantly reduced the growth of oriental melon with increasing A. gossypii density level. Plant growth was decreased by 63${\sim}$69%, 62${\sim}$88% and 49${\sim}$70% in plant height, leaf area and dry weight, respectively. During 10 day after aphids removal, the infested leaves remained stunt, however, new shoot and leaf recovered gradually. By 20 day, plant height, leaf area and dry weight substantially increased in 5 aphid per plant. Plant recovered rapidly with day and among aphid density level, 5 aphid per plant showed rapid recovery. When plant were infested with several density of aphid per plant, sugar contents of total leaves were not significantly different between aphid density level. Whereas, sugar contents of lear infested with aphid per leaf were decreased with increasing cumulative aphid-days.

Comparing the susceptibilities of green peach aphid populations against several insecticides

  • Min, Ji-Hyun;Yoon, Heon;Kwon, Hay-Ri;Yu, Yong-Man;Youn, Young-Nam
    • Korean Journal of Agricultural Science
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    • v.44 no.3
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    • pp.348-358
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    • 2017
  • The green peach aphid, Myzus persicae, is one of the most serious insect pest and a vector for a multitude of viral diseases to many crops, vegetables, ornamentals, and fruit trees in the world. A large number of aphids can reduce plant vigor and cause defoliation. Many insecticides have been developed and applied to control the green peach aphid. However, this aphid has displayed a remarkable ability to establish resistance to almost every insecticide. We treated 5 different insecticides registered for M. persicae on pepper leaves and investigated the effects of the insecticides by measuring the time it took to achieve 90% control of the aphids. Acetamiprid worked faster than any other insecticides while cyantraniliprole showed the slowest insecticidal effect. Pymetrozine, pyrifluquinazon, and spirotetramet provided 90% control within similar time. Iwol population's control value was higher than any other populations 24 hours after treatment. When five different unregistered insecticides for M. persicae were treated on pepper leaves, no insecticidal effect was found for gamma-cyhalothrin and novaluron and spinosad showed an insecticidal effect of up to 70% in Iwol population only. Although chlorfenapyr and dinotefuran were not registered for M. persicae, their insecticidal effects were found to be 90% or higher.

Karyotype and Insecticide Resistance of the Green Peach Aphids (Myzus persicae Sulzer) collected from Tobacco fields (연초포장에서 발생하는 복숭아혹진딧물의 핵형과 살충제 저항성)

  • 채순용;김영호;김상석;장영덕
    • Journal of the Korean Society of Tobacco Science
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    • v.19 no.2
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    • pp.107-116
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    • 1997
  • Karyotype of apterous green peach aphid (Myzus persicae SuIBer) populations occurring in tobacco fields and their resistance to insecticides were investigated. All of the 16 clones collected from tobacco fields had same chromosome number of 2n=12. Among them, 5 clones had the normal karyotype, regardless of color morph, but chromosomal translnations between #1 and #3 autosomal chromosomes were noted in the other 11 clones. All of the aphid clones were highly resistant tolambda cyhalothrin, a pyrethroid pesticide, having over 20 times higher concentrations of LD90 than recommended concentration (10ppm) for the aphid. However, their resistance to acephate, demeton 5-methyl and pirimicarh varied depending on the clones, among which green-colored clones were generally more resistant to the pesticides than red-colored ones. No association was found between the insecticide resistance and the karyotype of the aphid in this experiment.

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Biological Characteristics of the Aphid-eating Gall-midge, Aphidoletes aphidimyza (Diptera: Cecidomyiidae) as a Biological Control Agents of Aphids (진딧물천적인 진디혹파리(Aphidoletes aphidimyza)의 생물학적 특성)

  • 정영석;최용석;오인석;한규홍;서미자;윤영남
    • Korean journal of applied entomology
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    • v.42 no.3
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    • pp.241-248
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    • 2003
  • The aphid-eating gall-midge, Aphidoletes aphidimyza (Rondani), as predator of aphids was wildly used as one of the biological control agents for control of several kinds of aphids. Their some kinds of biological characteristics were examined. The developmental periods of A. aphidimyza from egg to adult with the green peach aphid as prey were 40 and 12 days at 15$^{\circ}C$ and 30$^{\circ}C$, respectively. Supplying the cotton aphid as prey, their developmental periods were shorter than with the green peach aphid. In case of the preservation of pupa in the cold condition, emergence rates were over 90% for 1 and 2 weeks at 5$^{\circ}C$ and l0$^{\circ}C$, respectively. Adult females of A. aphidimyza began mating and laying eggs at 2-3 days after emergence, and they laid about 200 eggs for lo days of average life span. Most of adults were emerged from pupa at 6 to 8 pm during a day, and they mainly acted in the early night.