• 제목/요약/키워드: antipyretic

검색결과 206건 처리시간 0.028초

HaCaT cell에서 황련 추출물(Coptis chinensis)의 피부보습과 항염증 효과 (Skin Moisturizing Properties and Anti-Inflammatory effects of extracts from Coptis chinensis in HaCaT cells)

  • 김은희;문영란;장영아
    • 한국응용과학기술학회지
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    • 제38권3호
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    • pp.870-882
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    • 2021
  • 황련은 한의학에서 진정, 소염, 항균 및 해열 등의 여러 질병의 치료에 이용되어져 왔다. 본 연구에서는 UVB와 TNF-α/IFN-γ로 유도된 HaCaT 세포에서 피부장벽과 염증관련 인자에 대한 황련 열수 추출물(CCW)의 효과를 조사하고, 보습 및 항염증 소재로서의 가능성을 알아보고자 하였다. 각 질형성세포인 HaCaT 세포에서 CCW의 보습 및 항염 효과를 확인하기 위해 MTT assay를 통한 시료의 독성 평가를 실시하였다. 이를 바탕으로, HA(Hyaluronic acid) 생성량과 filaggrin의 protein 및 mRNA 발현을 측정하였다. TNF-α/IFN-γ로 자극된 HaCaT 세포에서 CCW는 농도 의존적으로 HA의 생성량을 증가 시켰고, filaggrin의 protein 및 mRNA 발현 측정 결과 CCW의 농도 증가에 따라 발현율도 증가하였다. UVB로 유도된 HaCaT 세포에서 CCW는 ROS의 생성을 감소 시켰고, 양성대조군(positive control)인 EGCG( (-)-epigallocatechin-3-gallate)와 유의한 결과를 보여 주었다. 또한, CCW는 염증성 사이토카인 TNF-α, IL-1β, IL-6, IL-8의 발현을 억제 시켰고, TNF-α/IFN-γ로 자극된 HaCaT 세포에서 피부 염증에 따른 주요 인자인 COX-2의 protein 및 mRNA 발현이 농도 의존적으로 감소되는 것을 확인할 수 있었다. 이러한 결과는, 황련 열수 추출물의 보습 및 항염증 효과를 가지는 화장품 소재로서의 가치를 제안할 수 있다.

인간 섬유육종세포에서 비쑥 추출물과 유기용매 분획물의 암전이 억제 효과 (Anti-invasive Effect of Artemisia scoparia Halophyte Extract and its Solvent-partitioned Fractions in Human Fibrosarcoma Cells)

  • 김준세;공창숙;심현보;서영완
    • 생명과학회지
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    • 제31권12호
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    • pp.1100-1109
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    • 2021
  • 염생식물인 비쑥은 식용이 가능한 약용식물로서 살충, 항염, 항콜레스테롤, 해열, 항균 활성 등이 알려져 있다. 본 연구에서는 phorbol-12-myristate-13-acetate (PMA)로 자극된 인간 섬유육종 HT-1080 세포에서 5가지 활성검색방법 즉 : gelatin zymography, MMPs ELISA, wound healing assay, reverse transcription-polymerase chain reaction (RT-PCR), Western blot을 이용하여 비쑥의 조추출물과 그 용매 분획물의 MMP-2와 MMP-9 효소 활성에 대한 저해 효과를 평가하였다. 비쑥 시료들을 메틸렌 클로라이드(MC)와 메탄올(MeOH)로 각각 2번 추출하여 합한 것을 조추출물로 사용하였으며 이 조추출물은 MMP-2와 MMP-9 효소활성에 대해 유의한 억제 효과를 나타내었다. 이 조추출물은 용매극성에 따라 다시 n-hexane, 85% aq.MeOH, n-butanol 및 물 분획층으로 분획되었으며 이렇게 얻어진 4개의 용매 분획물들중에 n-hexane과 85% aq.MeOH 분획이 gelatin zymography와 MMP ELISA assay에서 MMP-2와 MMP-9의 활성을 효과적으로 억제하였다. 또한 wound healing assay, RT-PCR 및 Western blot assay에서 H2O 분획을 제외한 모든 용매 분획물들이 세포 이동, 그리고 MMP-2 및 MMP-9의 mRNA와 단백질 발현을 유의하게 억제하였다.

제주지역 갯메꽃(Calystegia soldanella L.) 유래 부정근 재분화 및 생물반응기 시스템 이용 대량증식법 (Regeneration of adventitious root from Calystegia soldanella L. in Jeju island and mass proliferation method using bioreactor system)

  • 이종두;장은비;윤원종;정용환
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2021년도 춘계학술대회
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    • pp.37-37
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    • 2021
  • Calystegia soldanella L. is a perennial herbaceous halophyte belonging to the convolvulaceae family, which mainly grows in coastal sand dunes in Korea. Shoots and rhizomes are edible, and roots called 'Hyoseon Chogeun' are known to have medicinal effects such as antipyretic, sterilization, and diuretic. In addition, physiological activities of antioxidant, anti-inflammatory, antiviral, antifungal and PTP-1B (protein tyrosine phosphate-1B) inhibition have been reported. In this study, in vitro induction cell lines of C. soldanella L. collected from the coastal sand dunes in Jeju island was redifferentiated into adventitious roots that can be used as medicinal resources. Also the biomass of mass-proliferated adventitious roots using a bioreactor were evaluated. Plants of C. soldanella L. were collected from the crevice of the seashore in the coastal area of Taeheung 2-ri, Namwon-eup, Seogwipo-si. Then, it was separated into leaves, stems, rhizomes, and roots, and surface sterilized with 70% ethyl alcohol and 2% NaOCl (sodium hypochlorite). After washing with sterilized water, each organ section was cultured in Hormone-free MS medium (Murashige & Skoog Medium). As a result, the induction response rates were evaluated at 85% and 55%, respectively, in terms of callus formation and shoot generation in the rhizome segment. In the case of the adventitious roots morphological characteristics induced by single-use treatment of auxin-based plant growth regulators IBA and NAA from redifferentiated shoots were compared. Most efficient adventitious root culture method as a rooting rate, number, length, and biomass proliferation in the bioreactor system was confirmed when treated by culturing in MS salts, Sucrose 30 g·L-1, and IBA 1mg·L-1 for 4 weeks. In this study, the medium composition and culture period were confirmed using a bioreactor system to mass-proliferate adventitious roots derived from C. soldanella L. in Jeju island. Also this adventitious root line developed a new medicinal material could increase value of the bio-industry ingredient through quantitative and qualitative screening of phyto-bioactive compounds.

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한국 청소년의 약물남용과 비행행위

  • 김성이
    • 한국인구학
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    • 제11권2호
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    • pp.54-66
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    • 1988
  • I. Introduction Since the 1970's drug abuse among young people has increasingly become a social problem in Korea. In the 1980's, drug abuse, especially glue sniffing, has become the cause of many unfortunated incidents resulting in harm to others as well as the abusers themselves. Taking into consideration of the seriousness of this problem, the Republic of Korea National Red Cross initiated a nation-wide research programme, to understand the present situation and to raise the level of public awareness. The goal of this research was to begin a nation - wide campaign against drug abuse. The research team was composed of the Advisary Committee members and the staff of the Youth Department of the Republic of Korea National Red Cross. The data were collected in February 1988 with the collaboration of the staff and volunteers in the local Chapters. The respondents were allocated nation-wide by the quota sampling method. The questionnaires were distributed to the respondents in three groups :2, 700 to junior and senior high school students, 605 to working youths, and 916 to delinquent youths. A total of 4, 221 questionnaires were collected. II. Characteristics of the Respondents The respondents in each group were selected evenly from rural and urban areas. The general characteristics of the respondents can be described as follow: in case of students, the proportions between male and female respondents, and between senior high school and junior high school students were almost evenly distributed. In case of working youths, the proportion of females (80.5%) was higher than those of the students and the delinquents groups. Delinquent youths were defined as those currently being under custody of the centers for juvenile delinquents. Of this number, 38.8% and 68.2% were junior and senior high school drop-outs respectively. The majority of them (92.6%) were male. As for the family background of the respondents, the proportion of those residing in poverty - stricken areas, and the proportion of those from broken families were higher in case of working youths and delinquent youths than those in case of students. III. Present Patterns of Drug Abuse The following summarizes the presents of drug abuse, as tabulated from the results of the survey. 1. Smoking The percentage of youths who smoke was 36% in the student group, 32% m the working youths group, and 94.4% in the delinquent youths group. 2. Alcohol 50.3% of students, 71.6% of working youths, and 93.3% of delinquent youths has experienced drinking alcohol beverages. 3. Tonic: non - alcoholic, caffeinated beverages popular in Korea and Japan The percentage of those who have used tonic at least once was over 90% in all of the three groups. 4. Sedative About 70% of each group has used sedative with the proportion of working youths use higher than those in other groups. 5. Stimulants Those who have used stimulants comprised around 15% in each group. 6. Tranquilizers Somewhat less than 5% of students and working youths, and 28% of delinquent youths, have used tranquilizers. 7. Hypnotics The users of hypnotics amounted to 0.4% of students, 2.6% of working youths and 7.1% of delinquent youths. 8. Marihuana Those who have used marihuana indicated 0.7% of students, 0.8% of working youths, and 13% of delinquent youths. 9. Glue-sniffing The percentage of glue-sniffing was 3.7%, 5% in the students group and in the youths group respectively, but the proportion was unusually high, at 40.7% in the delinquent youths group. From the results of the survey the present situation of drug abuse in Korea can be summarized as follows: 1. A high percentage of Korean youths have experienced smoking cigarettes and drinking alcoholic beverages. 2. Tonics (non - alcoholic, caffeinated beverages), antipyretic analgesics and stimulants quite regularly used. 3. Tranquilizers, hypnotics, marihuana and glue-sniffing are more widely used among delinquent youths than the other youths. From this fact, there exists a correlation between drug abuse and juvenile delinquency. IV. Time-series Analysis of the First Experience of Drug Abuse and Deviant Behaviour The respoundents were asked when they were first exposed to drugs and when they committed deviant acts. By calculating the average age of each experience, the following pattern was found (See Figure 1). Youths are first exposed to drugs by abuse of tonic(non - alcoholic, caffeinated beverages). At the age of 13, they amoke cigarettes, the use of antipyretic analgesics begins at 14 year old, while at the age of 15, they use tranquilizers, and at 16 hynotics. The period of drug abuse which starts from drinking caffeinated beverages and smoking cigarettes and ends in the use of hypnotics takes about three years. During this period, other delinquent behaviours begin to surface, that is, at the age of 13 when smoking cigarettes begins, the delinquent behaviour pattern starts with truancy. Next, they start taking money from others by using physical force. Prior to the age of 15, they are suspended from school, become hostile to adults, begin running away from home, and start using stimulants and alcohol. Soon they become involved even in glue-sniffing and in the use of marihuana. At the age of 15, they begin to see adult videos and carry weapons. Sexual promiscuity and usage of tranquilizers follows the viewing of adult videos. Consequently, by the time they reach the age of 16, they visit drinking establishments, and are picked up by police for committing delinquent acts. And finally, they come to use hypnotic - type drugs. From the above descriptions, drug abuse can be assumed to have a close correlation with delinquent behaviour. V. Social Factors Related to Drug Abuse As for the Korean youths, glue-sniffing is found to he related to aggressive delinquency, in such cases as run - aways, being picked up by the police, and taking money by force. Smoking cigarettes and drinking alcohol is found to be related to seeing adult videos and visiting drinking establishments. Hypnotics and marihuana were found to be representive of drugs which are related to degenerational delinquency, irrespective of social delinquency. The social factors connected with these drug abuse are as follows: 1. Individual factors Male students were more heavily involved in the usage of drug than females. Youths who do not attend church were more likely to be involved in drugs than those who attend. 2. Family factors The youths who were displeased with their mothers smoking and those who thought their parents did not love each other, or those whose parents had used drugs without prescription, were more likely to he drug users. 3. School factors Those youths who found school life boring, were unsuccessful in their studies, spend most of their time with friends, feel their teachers smoke too much, those who had a positive perception of their teachers smoking were likely to he drug users. To sum up, drug abusers depend on the influence of their parents, teachers and peers. IV. Reasons for Drug Abuse Korean students have mainly used drugs to release stress (42.8%), to stay awake (19.7%), and because of the easy accessibility of drugs( 16.6%). Other reasons are due to their ignorance of the side effects of the drugs (3.6%), natural curiosity (4.2%), and to increase strength(3.O%). From the above facts, the major reasons for drug abuse among Korean youths are to release stress and to stay awake in order to prepare exams. Furthermore, since drugs are readily available, we can conclude that drug abuse is caused by the school system(such as entrance exams) in Korea. VII. Conclusion Drug usage among Korean youths are relatively less common than those of western youths. In some cases, such as, glue-sniffing and use of stimulants, the pattern of drug abuse is found. Moreover, early drug abuse is evident, and it has a close connection with deviant behaviour, resulting in juvenile delinquency. Drug abuse cannot be attributed to any one social factor. Specifically, drug abuse depends on parents, peers, teachers and other members of the community, and also is influenced by social institutions such as the entrance exam system. Every person and organization concerned with youth must participate collectively in restraining drug abuse. Finally, it is suggested that social agencial working for youth welfare should make every effort to tackle this serious problem confronted by the Korean youths today.

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황련(Coptis chinensis) 추출물의 항균, 항진균 효과와 BCOP 분석을 이용한 안전성 검증 (Antimicrobial, antifungal effect and safety verification using BCOP assay of extracts from Coptis chinensis)

  • 김은희;장영아;김솔비;김한혁;이진태
    • Journal of Applied Biological Chemistry
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    • 제61권3호
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    • pp.297-304
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    • 2018
  • 황련은 한의학에서 진정, 소염, 항균 및 해열에 쓰이고 있으며, 주성분인 berberine은 강력한 항균작용을 가지는 것으로 알려져 있다. 본 연구에서는 황련에 대한 피부상재균 및 주거환경의 실내 낙하균에 대한 항균효과와 주거 환경에서 검출빈도가 높은 곰팡이에 대한 항진균 효과 및 인체에 대한 안전성을 조사하였다. 황련 열수 추출물(CW)은 Propionibacterium acnes, Staphylococcus aureus, Staphylococcus epidermidis에 대해 항균 효과를 보였으며, 각기 다른 4곳에서 포집된 실내 낙하균에 대해서도 항균효과를 보였다. 100 mg/mL의 농도에서 42일까지 항균의 지속력과 열처리 후에도 항균력의 변화가 없는 열 안정성을 보여주었다. CW의 MIC와 MBC는 S. aureus는 0.03, 0.05 mg/mL, S. epidermidis는 0.50, 0.75 mg/mL, P. acne는 0.10, 0.15 mg/mL 였다. 주변 환경에서 검출빈도가 높은 5종의 곰팡이 중 4종에 대해 항진균 활성을 보였으며, 균사 생장의 100%를 억제할 수 있는 최저 농도로 결정하여 MIC를 측정한 결과 Gliocladium virens는 65 mg/mL이었다. Aureobasidium pullulans에 대한 MIC는 90 mg/mL, Penicilium pinophilum 및 Chaetomium globosum에 대한 MIC는 100 mg/mL로 측정되었다. 피부자극 테스트인 패치테스트와 안자극 테스트인 ocular mucous membrane irritation evaluation test에서도 CW는 자극을 보이지 않는 안전한 추출물로 간주되었다. 따라서, 이러한 결과는, 황련(Coptis chinensis)은 항균, 항진균 활성 및 인체 안전성을 가지는 화장품 및 주거 환경 산업의 소재 개발에 응용될 수 있음을 시사한다.

인삼 및 인삼 사포닌이 쥐의 건망증 및 신경세포배양에 미치는 영향 (Effects of Ginseng and Its Saponins on Experimental Amnesia in Mice and on Cell Cultures of Neurons)

  • Saito Hiroshi;Nishiyama Nobuyoshi;Iwai Akihiko;Kawajiri Shinichi;Himi Toshiyuki;Sakai Toshimi;Fukunaka Chizu
    • 고려인삼학회:학술대회논문집
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    • 고려인삼학회 1988년도 학술대회지
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    • pp.92-98
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    • 1988
  • 본 연구는 중앙대서전에 인삼의 주 효과중 건망증 방지작용이라고 수록되어 있는 것을 확인하기 위하여 행하여졌다. Step through 시험에서 $GRb_1$$GRg_1$은 기억력 획득을 용이하게 하였으며 전기충격 쇼크에 의해 야기되는 기억상실 효과를 억제하였다. 게대가 $Rg_1$은 에타놀 야기 기억재생 저해작용을 방지하였다. Stepdown test에 있어서 $Rb_1,\;Rb_2,\;Rg_1$은 전기충격쇼크에 의해 야기되는 기억보지 저해효과를 방지하였다. 또한 $Rg_1$은 에타놀에 의해 야기되는 기억재생 저해효과를 방지하였으며 단기적 기억력 획득을 용이케 하였다. Shuttle box와 lever press 시험에서도 $Rb_1$을 제외하고 나머지는 기억획득과 재생에 효과를 미치지 못했다. $Rb_1$은 shuttle box 시험에서 조건회피반응의 재생 (retrieval)을 억제하였다. 이와 같은 4가지 시험이 끝난후에 인삼의 구강투여가 진정, 진통, 해열, 진경효과와 자발 및 탐색활동에 미치는 영향을 500 mg/kg 투여 범위내에서 조사하였으나 아무런 반응도 관찰되지 않았다. 현재까지 연구결과는 $Rg_1$이 기억의 재생 및 학습반응의 획득과정에 대하여 효과가 있다는 것을 의미한다. 최근의 신경성장인자(NGF)가 성숙동물에 있어 뇌신경세포의 생존, 재생 및 조절에 미치는 작용에 관한 연구결과는 지능장해와 건망증에 대한 신경성장인자의 중요성을 시사했다. NGF에 의해 야기된 신경세포돌기 성장의 특이성에 관한 연구결과를 병아리 배배근 신경절에 있어서 NGF의 영향은 $Rh_1$에 의해 증가되었다. 다음으로 $Rb_1$은 병아리 배의 감각 및 교감신경단위에 있어서 NGF-관련 신경섬유 증가를 강화시켰다. NGF와 $Rb_1$을 함께 했을 때도 역시 쥐 대뇌피질의 신경세포 생존수를 증가시키는 경향을 보였다. NGF는 쥐의 배격부 부근의 신경세포를 배양했을 때 cholineacetyl transferase 활성을 증가시키지 않았다. 이러한 결과로 $Rb_1$은 뇌에 있어서 신경세포의 생존이나 재생에 중요한 역활을 한다는 것은 알 수 있었다.

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