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http://dx.doi.org/10.3839/jabc.2018.042

Antimicrobial, antifungal effect and safety verification using BCOP assay of extracts from Coptis chinensis  

Kim, Eun-Hee (Department of Cosmeceutical Science, Daegu Haany University)
Jang, Young-Ah (Department of Cosmeceutical Science, Daegu Haany University)
Kim, Sol-Bi (Department of Cosmeceutical Science, Daegu Haany University)
Kim, Han-Hyuk (Medical Convergence Textile Center, Reasearch & Certification Team, Gyeongbuk Technopark)
Lee, Jin-Tae (Department of Cosmeceutical Science, Daegu Haany University)
Publication Information
Journal of Applied Biological Chemistry / v.61, no.3, 2018 , pp. 297-304 More about this Journal
Abstract
Coptis chinensis is used in oriental medicine for soothing, anti-inflammation, antimicrobial and antipyretic properties, and its main ingredient berberine is known to have strong antibacterial activity. In this study, we investigated the anti-microbial effect of hot water extract of Coptis chinensis (CW) on skin related microorganism and the airborne microbe, the antifungal effects of fungi, which are frequently detected in residential environments. CW showed antibacterial effect against Propionibacterium acnes, Staphylococcus aureus and Staphylococcus epidermidis, against the airborne microbe, which was collected in four different places. At the concentration of 100 mg/mL, the antimicrobial activity continued for 42 days, showed heat stability without change in the antimicrobial activity even after heat treatment. The MIC and MBC of CW against S. aureus was 0.03, 0.05 mg/mL, against S. epidermidis was 0.50, 0.75 mg/mL and against P. acne was 0.10, 0.15 mg/mL. As a result of measuring the MIC of four kinds of fungi with high detection frequency in the surrounding environment, Gliocladium virens was 65 mg/mL by determined as MIC which can inhibit one hundred percent of mycelial growth. The concentration 90 mg/mL was determined as MIC against Aureobasidium pullulans and 100 mg/mL against Penicilium pinophilum and Chaetomium globosum. CW was considered a safe extract that showed no irritation even in the ocular mucous membrane irritation evaluation test, a patch test. Therefore, these results suggest that Coptis chinensis has antimicrobial, antifungal and safety on human body and can be applied to the development of materials for cosmetic and residential environment industries.
Keywords
Alternative methods; Antifungal; BCOP assay; Human skin related microorganisms; Thermal stability;
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