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http://dx.doi.org/10.5352/JLS.2021.31.12.1100

Anti-invasive Effect of Artemisia scoparia Halophyte Extract and its Solvent-partitioned Fractions in Human Fibrosarcoma Cells  

Kim, Junse (Ocean Science and Technology School, Korea Maritime and Ocean University)
Kong, Chang-Suk (Department of Food and Nutrition, College of Medical and Life Sciences, Silla University)
Sim, Hyun-Bo (Incheon Academy of Sicenc and Arts)
Seo, Youngwan (Ocean Science and Technology School, Korea Maritime and Ocean University)
Publication Information
Journal of Life Science / v.31, no.12, 2021 , pp. 1100-1109 More about this Journal
Abstract
The halophyte Artemisia scoparia is an edible medicinal plant, with insecticidal, anti-inflammatory, anticholesterol, antipyretic, and antibacterial effects. The aim of this study was to assess the inhibitory effect of crude extract and solvent-partitioned fractions obtained from A. scoparia on MMP-2 and MMP-9 activity in phorbol-12-myristate-13-acetate (PMA)-stimulated human fibrosarcoma HT-1080 cells using four different activity tests: gelatin zymography, MMP enzyme-linked immunosorbent assay (ELISA), wound healing assay, reverse transcription-polymerase chain reaction (RT-PCR), and Western blot assay. A. scoparia samples were extracted twice with methylene chloride (MC) and twice with methanol (MeOH). After the MC and MeOH crude extracts were combined, the combined crude extracts showed a significant inhibitory effect against MMP-2 and MMP-9 enzymes. They were then fractionated into n-hexane, 85% (v/v) aqueous methanol (85% (v/v) aq.MeOH), n-butanol, and water according to solvent polarity. Among the four solvent-partitioned fractions, n-hexane and 85% (v/v) aq. MeOH fractions significantly inhibited MMP-2 and MMP-9 activity and cell mobility. In addition, the n-hexane and 85% (v/v) aq.MeOH fractions effectively inhibited MMP-2 and -9 activity in the gelatin zymography and MMP ELISA assay. In the wound healing assay, RT-PCR, and Western blot assay, all solvent-partitioned fractions, except the H2O fraction, significantly suppressed cell migration, as well as the expression levels of MMP-2 and -9 mRNA and proteins.
Keywords
Anti-invasive; Artemisia scoparia; cell mobility; HT-1080; MMP inhibition;
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1 El-Feraly, F. S. and Chan, Y. M. 1978. Isolation and characterization of the sesquiterpene lactones costunolide, parthenolide, costunolide diepoxide, santamarine, and reynosin from Magnolia grandiflora L. J. Pharm. Sci. 67, 347-350.   DOI
2 McCawley, L. J. and Matrisian, L. M. 2001. Matrix metalloproteinases: they're not just for matrix anymore. Curr. Opin. Cell Biol. 13, 534-540.   DOI
3 Negahban, M., Moharramipour, S. and Sefidkon, F. 2006. Chemical composition and insecticidal activity of Artemisia scoparia essential oil against three coleopteran stored-product insects. J. Asia-Pac. Entomol. 9, 381-388.   DOI
4 Ryu, K. J., Yoou, M. S., Seo, Y., Yoon, K. W., Kim, H. M. and Jeong, H. J. 2018. Therapeutic effects of Artemisia scoparia Waltst. et Kitaib in a murine model of atopic dermatitis. Clin. Exp. Dermatol. 43, 798-805.   DOI
5 Shim, H. B., Lee, K. M., Kim, W., Nam, G. H., Sim, S. and Lee, B. Y. 2017. Coastal Plants of Korea, pp. 31-33, National Institute of Biological Resources: Incheon, Korea.
6 Jablonska-Trypuc, A., Matejczyk, M. and Rosochacki, S. 2016. Matrix metalloproteinases (MMPs), the main extracellular matrix (ECM) enzymes in collagen degradation, as a target for anticancer drugs. J. Enzyme Inhib. Med. Chem. 31, 177-183.   DOI
7 Kim, J., Kong, C. S. and Seo, Y. 2018. Inhibitory effect of Carex pumila extracts on MMP-2 and MMP-9 activities in HT-1080 cells. Ocean Polar Res. 40, 249-257.
8 Al-Attas, A. A., El-Shaer, N. S., Mohamed, G. A., Ibrahim, S. R. and Esmat, A. 2015. Anti-inflammatory sesquiterpenes from Costus speciosus rhizomes. J. Ethnopharm. 176, 365-374.   DOI
9 Kumar, G. B., Nair, B. G., Perry, J. J. P. and Martin, D. B. C. 2019. Recent insights into natural product inhibitors of matrix metalloproteinases. Med. Chem. Commun. 10, 2024-2037.   DOI
10 Adegawa, S., Miyase, T. and Ueno, A. 1987. Sesquiterpene lactones from Diaspananthus uniflorus (SCH. BIP.) KITAM. Chem. Pharma. Bull. 35, 1479-1485.   DOI
11 Fang, F., Sang, S., Chen, K. Y., Gosslau, A., Ho, C. T. and Rosen, R. T. 2005. Isolation and identification of cytotoxic compounds from Bay leaf (Laurus nobilis). Food Chem. 93, 497-501.   DOI
12 Habib, M. and Waheed, I. 2013. Evaluation of anti-nociceptive, anti-inflammatory and antipyretic activities of Artemisia scoparia hydromethanolic extract. J. Ethnopharmacol. 145, 18-24.   DOI
13 Hannocks, M. J., Zhang, X., Gerwien, H., Chashchina, A., Burmeister, M., Korpos, E., Song, J. and Sorokin, L. 2019. The gelatinases, MMP-2 and MMP-9, as fine tuners of neuroinflammatory processes. Matrix Biol. 75-76, 102-113.   DOI
14 KIOSTAT. 2021. Press release on the statistical results of the cause of death in 2020. 1-56.
15 Oh, J. H., Karadeniz, F., Seo, Y. and Kong, C. S. 2018. Artemisia scoparia inhibits adipogenesis in 3T3-L1 pre-adipocytes by downregulating the MAPK pathway. J. Life Sci. 28, 999-1006.   DOI
16 Singh, H. P., Kaur, S., Mittal, S., Batish, D. R. and Kohli, R. K. 2008. Phytotoxicity of major constituents of the volatile oil from leaves of Artemisia scoparia Waldst. & Kit. Z. Naturforsch. C 63, 663-666.
17 Yahagi, T., Yakura, N., Matsuzaki, K. and Kitanaka, S. 2014. Inhibitory effect of chemical constituents from Artemisia scoparia Waldst. et Kit. on triglyceride accumulation in 3T3-L1 cells and nitric oxide production in RAW 264.7 cells. J. Nat. Med. 68, 414-420.   DOI
18 National Cancer Center (NCC). 2020. Annual report of cancer statistics in Korea in 2018. 1-231.
19 Nam, S. Y., Han, N. R., Rah, S. Y., Seo, Y., Kim, H. M. and Jeong, H. J. 2018. Anti-inflammatory effects of Artemisia scoparia and its active constituent, 3,5-dicaffeoyl-epi-quinic acid against activated mast cells. Immunopharm. Immunot. 40, 52-58.   DOI
20 Cho, J. Y., Park, J., Yoo, E. S., Baik, K. U., Jung, J. H., Lee, J. and Park, M. H. 1998. Inhibitory effect of sesquiterpene lactones from Saussurea lappa on tumor necrosis factor-α production in murine macrophage-like cells. Planta Med. 64, 594-597.   DOI
21 Yoshikawa, M., Shimoda, H., Uemura, T., Morikawa, T., Kawahara, Y. and Matsuda, H. 2000. Alcohol absorption inhibitors from bay leaf (Laurus nobilis): structure-requirements of sesquiterpenes for the activity. Bioorg. Med. Chem. 8, 2071-2077.   DOI
22 Verma, R. P. and Hansch, C. 2007. Matrix metalloproteinases (MMPs): chemical-biological functions and (Q) SARs. Bioorg. Med. Chem. 15, 2223-2268.   DOI
23 Visse, R. and Nagase, H. 2003. Matrix metalloproteinases and tissue inhibitors of metalloproteinases: structure, function, and biochemistry. Circ. Res. 92, 827-839.   DOI