• 한국식품조리과학회지
• /
• 제32권4호
• /
• pp.383-389
• /
• 2016

Purpose: This study was conducted to evaluate the effect of rehydration and subsequent heating at high temperature on the pigments and antioxidants of dried samnamul (Aruncus dioicus) and daraesoon (Actinidia arguta). Methods: Rehydration included 16 h-soaking in cold water, and 30 min-boiling and 1 h-infusion in water. Rehydrated samnamul and daraesoon were heated at $180^{\circ}C$ for 10 or 20 min with or without perilla oil addition (10%) for cooking. Pigments and antioxidants were determined by HPLC and spectrophotometry. Results: Rehydration caused decreases in pigment and polyphenol contents, but increase in tocopherol content. Cooking by heating without addition of perilla oil resulted in increases in chlorophyll and carotenoid contents, but decreases in polyphenol and tocopherol contents. Decrease in tocopherol content by heating at $180^{\circ}C$ was reversed by the addition of perilla oil. Conclusion: This study strongly suggested that cooking of samnamul and daraesoon at $180^{\circ}C$ with perilla oil could improve color, texture, and potential health functionality by recovering the loss of antioxidants and pigments with antioxidant activity.

• Nuclear Engineering and Technology
• /
• 제14권2호
• /
• pp.70-77
• /
• 1982

전자선을 이용하여 저밀도 폴리에틸렌을 가교시킬때의 삼관능성 단위체와 산화방지제의 효과를 검토하였다. 가교촉진제로서는 삼관능성 단량체인 Trimethylolpropane triacrylate(TMPTA), Trimethylolpropane trimethacrylate(TMPTM)과 Triallyl cyanurate(TAC)가 사용되었고, 산화방지제로서는 Irganox 1010(Pentaerythritol-tetrakist[3-(3,5-di-t-butyl-4-hydroxyphenyl)-propionate]), Santo-nox R(4,4'-Thio-bis(3-methyl-6-t-butylphenol)), Nocrac D(N-phenyl-$\beta$-naphthylamine)와 Bisphenol A(4,4'-Iso-propylidene bisphenol)가 사용되었다. 삼관능성 단위체중에서 TMPTA가 폴리에틸렌의 가교도를 제일 크게 높였으며 또한 산화안정성도 부여했다. 산화방지제중에서 Nocrac D가 폴리에틸렌에 가장 적당한 것으로 나타났다.

• Animal Bioscience
• /
• 제36권3호
• /
• pp.385-403
• /
• 2023

Male fertility is affected by multiple endogenous stressors, including reactive oxygen species (ROS), which greatly deteriorate the fertility. However, physiological levels of ROS are required by sperm for the proper accomplishment of different cellular functions including proliferation, maturation, capacitation, acrosomal reaction, and fertilization. Excessive ROS production creates an imbalance between ROS production and neutralization resulting in oxidative stress (OS). OS causes male infertility by impairing sperm functions including reduced motility, deoxyribonucleic acid damage, morphological defects, and enhanced apoptosis. Several in-vivo and in-vitro studies have reported improvement in quality-related parameters of sperm following the use of different natural and synthetic antioxidants. In this review, we focus on the causes of OS, ROS production sources, mechanisms responsible for sperm damage, and the role of antioxidants in preserving sperm fertility.

• 한국동물생명공학회지
• /
• 제38권2호
• /
• pp.47-53
• /
• 2023

Despite numerous advances in in-vitro embryo production (IVP), many documented factors have been shown to influence the development of mammalian preimplantation embryos and the success of IVP. In this sense, elevated levels of reactive oxygen species (ROS) correlate with poor outcomes in assisted reproductive technologies (ART) due to oxidative stress (OS), which results from an imbalance between ROS production and neutralization. Indeed, excessive production of ROS compromises the structural and functional integrity of gametes and embryos both in vivo and in vitro. In particular, OS damages proteins, lipids, and DNA and accelerates cell apoptosis. Several in-vivo and in-vitro studies report an improvement in qualityrelevant parameters after the use of various antioxidants. In this review, we focus on OS and the source of free radicals and their effects on oocytes, sperm, and the embryo during IVP. In addition, antioxidants and their important role in IVP, supplementation during oocyte in vitro maturation (IVM), in vitro culture (IVC), and semen extenders were discussed. Nevertheless, various methods for determining the level of ROS in germ cells have been briefly described. Still, it is crucial to develop standardized antioxidant supplement systems to improve overall IVP success. Further studies should explore the safety, efficacy, mechanism of action, and combination of different antioxidants to improve IVP outcomes.

• 한국가축번식학회지
• /
• 제20권2호
• /
• pp.163-170
• /
• 1996

The effect of several potential antioxidants and amino acids were examined as a means of increasing the development of in vitro matured and in vitro fertilized oocytes into morulae or blastocysts. Bovine embryos developed to the 2~8 cell stage after in vitro fertilization were cultured for 5 to 6 days at 39$^{\circ}C$ in CR1aa containing varing concentraton of the antioxidants and amino acid in a gas phase consisting of 5% CO2, high humidified air. At 5~6 days, embryo developments were reduced, and embryos were fixed and stained with Hochest 33342 DNA stain to facilitate counting of cells. In experiment 1, the proportion of embryos developed to morulae and blastocysts in CR1aa containing 1mM, 2.5mM taurine (22.6% and 20.4%) was slightly higher than those of 0, 5 and 10mM Taurine (5.7, 5.7 and 3.9%, P<0.05). In experiment 2, addition of glutathione did not improve blastocyst development (P>0.05). In experiment 3, concentations of superoxide dismutase(SOD) ranging from 300 to 1,000 U did not affect the propotion of embryos developing into blastocysts (P>0.05). In experiment 4, addition of 250 U catalase(38.5%) was slighty higher than those of 0, 500 and 1,000U. In experiment 5, the proportion of embryo developed beyond morula stage in CR1aa with taurine plus EDTA was slighty higher than other treatments(15.7, 26.0 and 29.2%), there were no significantly increases in cell number among treatments(P>0.05). These results are indicating that antioxidants and amino acids can increase the proportion of embryos that develop into morulae and blastocysts, but did not increas in cell number of blastocysts.

• 한국가축번식학회지
• /
• 제20권2호
• /
• pp.171-177
• /
• 1996

This study was designed to evaluate the efficacy of antioxidants and amino acid with buffalo rat liver cell(BRLC), bovine oviductal epithelial cell(BOEC) and STOC monolayers in supporting the development of in vitro matured(IVM) and in vitro fertilized(IVF) bovine oocytes. Bovine embryos developed to the 2~8 cell stage after in vitro fertilization were cultured for 5 to 6 days at 39$^{\circ}C$ in CR1aa containing antioxidants and amino acids with various somatic cells. Embryo development was examined and cell numbers of blastocysts were counted by fluorescence staining method. In experiment 1, the proportion of embryos that reached the blastocyst stage in control, catalase(250U), SOD(600U), glutathione(100$\mu$M) and taurine(2.5mM) with BRLC were 11.4, 8, 0, 16.7 and 43.4 respectively. Taurine(2.5mM) with BRLC group was significantly the highest among treatments(P<0.05). In experiment 2, in vitro development rate into blastocyst in control, catalase(250U), SOD(600U), glutathione(100$\mu$M) and taurine(2.5mM) with BOEC were 15.8, 23.5, 22.8, 28.6 and 56.9 respectively. In experiment 3, embryonic development in all treatments as control, catalase(250U), SOD(600U), glutathione(100$\mu$M) and taurine(2.5mM) added to CR1aa with STO cells were 23.5, 24.5, 17.0, 28.8 and 50.0 blastocysts. These results show that antioxidants and amino acids with somatic cells can provide a significant benefit for coculture of early bovine embryos derived from IVM and IVF.

• 한국수산과학회지
• /
• 제4권1호
• /
• pp.22-30
• /
• 1971

Sea mussel Mytilus edulis, world-widely distributed, is a sort of popular food in Korea. The demands of high quality and mass production are not being satisfied since it is usually sun dried. A rapid dehydration and the treatment of food additives to improve the quality of the product seems to be required. In this paper, the effect of antioxidants and EDTA treatment was studied when sea mussel was pressed and hot-air dehydrated for 10 hours at 45 to $52^{\circ}C$ under the air flow rate of 3 meter per second. The results are as follows: 1) Cooked and pressed sea mussel was dehydrated mere rapidly than cooked without pressing. 2) The rehydration rate of pressed and dehydrated sea mussel was higher than that of the unpressed. 3) Among six samples, BHA treated material showed the best color preservation during dehydration and storage. The treatment of Teonox-II was also effective following BHA. 4) The effects of antioxidants used during dehydration and storage were in order of BHA, Tenox-II and NDGA treatment, while EDTA was ineffective. 5) No significant difference in rehydration rate was found between those treated with antioxidants or EDTA and the untreated. 6) The amount of soluble protein was higher in the samples treated with antioxidants than in EDTA treated or untreated samples. 7) The results suggest that the treatment of BHA and pressing process produce improved product in quality which gives higher amount of soluble protein and better rehydration, color preservation and preventive effect of rancidity.

• 식물조직배양학회지
• /
• 제22권2호
• /
• pp.65-70
• /
• 1995

자리공 모상근에서 betalain의 합성에는 광이 필수이지만 생장률은 암상태 보다 광상태(1500 1X)에서 5배 정도 억제된다. 따라서 광상태에서 모상근의 생장이 억제되는 원인을 규명하기 위해서 항산화제 처리에 따른 효과를 조사하였다. 항산화제(ascorbic acid, glutathione, $\alpha$-tocopherol, sodium pyrosulfate, propylgallic acid) 처리에 따른 모상근의 생장은 암상태에서 무처리보다 1.2-1.4배, 광상태에서 약 1.3-1.9배 생장이 증가되었으며, betalain 합성은 무처리보다 1.2-2.1배 향상되었다. 자리공 모상근의 성장 및 betalain 합성에서 항산화제 복합 처리에 따른 효과는 나타나지 않았다. Betalain 합성 과정에서 항산화제의 효과는 청색 파장하에서 가장 높게 나타났다. 따라서 광상태에서 자리공 모상근의 생장과 betalain 합성을 촉진시키기 위해서는 청색 파장에서 적절한 항산화제의 개발이 요구된다.

• 한국수산과학회지
• /
• 제33권3호
• /
• pp.238-242
• /
• 2000

소건 대멸치의 저장${\cdot}$유통 중 저장안정성을 부여하고자 건조 전항산화제를 처리하여 $37^{\circ}C$에 저장하였을 때 대멸치의 지질산화는 BHT를 첨가한 것이 현저하게 억제되었으며, 그 다음으로 ${\alpha}-trlcopherol$, rosemary 추출물, sage 추출물의 순이었다. 대멸치에 있어서 ${\alpha}-tocopherol$과 rosemary 추출물을 혼합처리한 항산화제의 항산화력은 BHT의 산화억제효과와 거의 비슷하였으며, 그 적정농도는 rosemary추출물 $0.1{\%}$와 $tocopherol\;0.1{\%}$를 혼합한 수준이었다. 건조한 대멸치를 $25{\circ}C$에 저장하였을 때 진공포장한 것이 항산화제의 산화방지 효과를 증대시키는 효과가 있었다.

• 한국식품영양과학회지
• /
• 제17권1호
• /
• pp.19-23
• /
• 1988

합성 항산화제인 BHA와 천연 항산화제인 ${\alpha}-tocopherol$, NDGA, propyl gallate, sesamol등의 항산화력을 비교하기 위하여 식용 대두유에 이들 항산화제를 첨가하여 가열처리한 후 2주간보존할때 일어나는 물리화학적인 변화와 지방산 조성의 변화를 조사하였다. 항산화력은 BHA > ${\alpha}-tocopherol$ > propyl gallate > sesamol의 순으로 BHA에 비하여 천연 항산화제가 다소 떨어졌지만 ${\alpha}-tocopherol$의 경우는 BHA에 크게 뒤지지 않는 우수한 황산화력을 나타내었다. Gas chromatography에 의한 지방산 분석의 결과 산패가 진행될수록 불포화도가 높은 linolenic acid 와 linolenic acid 의 함량은 증가하였으며 BHA와 ${\alpha}-tocopherol$을 첨가한 경우 linolenic acid와 linolenic acid 함량의 감소가 현저하게 억제되었다.