• 한국약용작물학회지
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• 제22권5호
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• pp.349-362
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• 2014

This study was designed to maximize extract yields of functional components in Angelica gigas Nakai by extrusion process. In addition, the optimizing condition of the extrusion process was established to increase the extraction of the functional components in A. gigas. The total polyphenol was increased by 40% compared to a control. And the optimizing condition was that the screw speed was 62.76 rpm, the amount of A. gigas powder was 34.79 kg/h, and the amount of water was 4.44 kg/h. The result of antioxidant activities of A. gigas extrusion, the inhibition of oxidization had the effect of 10.29 ~ 14.59% compared to a control. The content of decursin and decursinol angelate, which were index components, was 6.37%; it was increased by 16.64% compared to a control (5.31%) and showed a significantly difference (p < 0.05). And the optimizing condition was the screw speed 93.71 rpm, the amount of A. gigas powder was 28.67 kg/h, and the amount of water was 9.9 kg/h.

• Natural Product Sciences
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• 제9권2호
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• pp.68-72
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• 2003

Quercetin and its sugar conjugates are the most abundantly distributed bioflavonoids and represent the largest proportion of flavonols in the plant kingdom. The present study was undertaken to demonstrate the effect of quercetin on the role of reactive oxygen species (ROS) in the development of gastric ulcers in rats. Administration of quercetin in doses of 50, 100 and $200\;mg\;kg^{-1}$ twice daily for 5 days, showed dose dependent significant protection against ethanol (EtOH), aspirin (ASP), cold-restraint stress (CRS) and pylorus ligation (PL) -induced gastric ulcer models and the results were comparable with those elicited by sucralfate. The thiobarbituric acid reactive substances in the stomach mucosa, an index of lipid peroxidation and regulation of plasma corticosterone were significantly increased in CRS-induced gastric ulceration. The queroetin $(100\;mg\;kg^{-1})$ and reduced glutathione effectively inhibited gastric lesions induced by CRS with a significant decrease in the lipid peroxidation and plasma corticosterone. These results indicate that quercetin a bioflavonoid exerts its antiulcer effect in light of free radical scavenging and plasma corticosterone in cold restraint stress ulcers.

• Nutrition Research and Practice
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• 제7권6호
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• pp.430-438
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• 2013

Increased oxidative stress in obese diabetes may have causal effects on diabetic complications, including dyslipidemia. Lipopolysccharides (LPS) along with an atherogenic diet have been found to increase oxidative stress and insulin resistance. Cranberry has been recognized as having beneficial effects on diseases related to oxidative stress. Therefore, we employed obese diabetic animals treated with an atherogenic diet and LPS, with the aim of examining the effects of cranberry powder (CP) on diabetic related metabolic conditions, including lipid profiles, serum insulin and glucose, and biomarkers of oxidative stress. Forty C57BL/KsJ-db/db mice were divided into the following five groups: normal diet + saline, atherogenic diet + saline, atherogenic diet + LPS, atherogenic diet + 5% CP + LPS, and atherogenic diet + 10% CP + LPS. Consumption of an atherogenic diet resulted in elevation of serum total cholesterol and atherogenic index (AI) and reduction of high density lipoprotein (HDL)-cholesterol. However, with 10% CP, the increase in mean HDL-cholesterol level was close to that of the group with a normal diet, whereas AI was maintained at a higher level than that of the group with a normal diet. LPS induced elevated serum insulin level was lowered by greater than 60% with CP (P < 0.05), and mean serum glucose level was reduced by approximately 19% with 5% CP (P > 0.05). Mean activity of liver cytosolic glutathione peroxidase was significantly increased by LPS injection, however it was reduced back to the value without LPS when the diet was fortified with 10% CP (P < 0.05). In groups with CP, a reduction in mean levels of serum protein carbonyl tended to occur in a dose dependent manner. Particularly with 10% CP, a reduction of approximately 89% was observed (P > 0.05). Overall results suggest that fortification of the atherogenic diet with CP may have potential health benefits for obese diabetes with high oxidative stress, by modulation of physical conditions, including some biomarkers of oxidative stress.

• 한국식품과학회지
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• 제31권2호
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• pp.361-367
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• 1999

항산화작용 및 항균작용이 있다고 알려져 있는 녹차 물추출물을 이용하여 조미오징어의 품질을 개선하기 위하여 분무하여 수분함량을 조정한 후 $30^{\circ}C$에서 보관하면서 저장중 갈색도의 변화를 측정하였다. 갈색도의 측정으로는 물추출색소, 5% KOH-methanol 추출색소, 색차계 등의 방법을 사용하였다. 물추출색소 및 5% KOH-methanol 추출색소를 이용하여 측정하였을 경우 처리구간의 높은 상관관계를 나타내어 적절한 갈색도 측정방법으로 생각되었다. 녹차의 물추출물을 분무한 처리구의 경우 저장기간에 따라 TBA가의 변화속도가 대조구보다 다소 느리게 측정되었으며 총미생물수도 낮게 측정되어 조미오징어 제조시에도 항산화작용 및 항균작용이 존재함을 알 수 있었다. 녹차 물추출물을 분무하여 조미오징어를 제조할 경우 수분함량을 다소 높여 조직감 개선에 기여할 수 있을 것으로 생각되었으며 갈변도 억제하므로 품질개선에 유효함을 알 수 있었다.

• Preventive Nutrition and Food Science
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• 제18권2호
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• pp.98-103
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• 2013

In this study, we evaluated the hypolipidemic and antioxidative effects of biotransformed soybean powder (BTS; Phellinus baumii-fermented soybean) on lipid metabolism in rats. Sprague-Dawley (SD) male rats were divided into basal diet group (BA), high fat diet group (HF), high fat diet containing 10% BTS group (10 BTS), and high fat diet containing 20% BTS group (20 BTS). Changes in the content of various isoflavones, including daidzein and genistein, within the soybean after fermentation to BTS were investigated. The levels of daidzein and genistein were $149.28{\mu}g/g$ and $364.31{\mu}g/g$, respectively. After six weeks experimental period, Food efficiency ratio in the 10 and 20 BTS group was significantly lower than the HF group (P<0.05). Total serum levels of cholesterol, triglycerides, low-density lipoprotein cholesterol, and atherogenic index ratio in the 10 or 20 BTS group were significantly lower than the HF group. The levels of alanine aminotransferase, aspartate aminotransferase and thiobarbituric acid reactive substance were significantly lower in the groups that received 10% and 20% BTS than the HF. The activities of SOD and CAT were significantly higher in the 10 and 20 BTS group than the HF group. The activity of XO in the 10 and 20 BTS group was significantly lower than in the HF group by 20% and 23%, respectively. In conclusion, these data suggest that BTS is an effective agent in improving lipid metabolism and antioxidant enzyme system.

• 한국식품영양과학회지
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• 제37권6호
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• pp.798-804
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• 2008

본 연구에서는 당근주스에서 분리한 Leuconostoc mesenteroides SM 균주와 비트 즙을 이용하여 비열처리 당근 주스를 이용한 젖산 발효 음료를 제조하였다. 상황버섯 추출액이 함유된 당근주스에 비트 즙을 $1{\sim}5%$(v/v) 수준으로 첨가하여 발효시킬 경우 설탕의 전환율은 대부분 97% 이상이었다. 발효 후의 점조도는 비트즙을 2% 수준으로 첨가하였을 때 0.55 $Pa{\cdot}s^n$로 가장 높았으며, 비트 즙의 첨가량이 3% 이상일 때부터 점조도는 감소하였다. 발효 후 냉장 저장시에 당근 젖산 발효 음료의 점조도는 저장 120시간까지 크게 증가하였다. 당근 젖산 발효 음료의 폴리페놀 함량과 DPPH에 의한 전자공여능은 비트 즙의 첨가량이 증가할수록 높게 나타나는 경향을 나타내었고, 비트 즙을 5% 첨가하였을 때 $114.27{\mu}g/mL$의 폴리페놀 함량과 65.4%의 전자공여능을 나타내었다. 당근 젖산 발효 음료를 냉장 저장 시에 저장 기간이 길어질수록 L값, a값, b값은 소폭 감소하였으나 비트 즙의 첨가로 붉은 자줏빛이 강화되어, 냉장 저장 2주후에도 당근 음료의 고유한 색이 유지되는 것으로 나타났으며, 생균수는 평균 $10^9$ CFU/mL 이상을 나타내었다.

• 한국축산식품학회지
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• 제35권5호
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• pp.660-668
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• 2015

This study was performed to investigate the chemical composition, mineral status, oxidative stability, and texture attributes of dry-cured ham from pigs fed processed sulfur (S, 1 g/kg feed), and from those fed a basal diet (CON), during the period from weaning to slaughter (174 d). Total collagen content and soluble collagen of the S group was significantly higher than that of the control group (p<0.05). The pH of the S group was significantly higher than that of the control group, whereas the S group had a lower expressible drip compared to the control group. The S group also showed the lower lightness compared to the control group (p<0.05). In regard to the mineral status, the S group had significantly lower Fe²⁺ and Ca²⁺ content than the control group (p<0.05), whereas the proteolysis index of the S group was significantly increased compared to the control group (p<0.05). The feeding of processed sulfur to pigs led to increased oxidative stability, related to lipids and pigments, in the dry-cured ham (p<0.05). Compared to the dry-cured ham from the control group, that from the S group exhibited lower springiness and gumminess; these results suggest that feeding processed sulfur to pigs can improve the quality of the texture and enhance the oxidative stability of dry-cured ham.

• 한국수정란이식학회지
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• 제30권1호
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• pp.23-31
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• 2015

Catalpol, an iridoid glucoside, isolated from the root of Rehmannia glutinosa Libosch. It possesses a broad range of biological and pharmacological activity including anti-tumor, anti-inflammation and anti-oxidant by acting as a free radical scavenger. Therefore, in this study, the effects of catalpol on blastocyst development, expression levels of reactive oxygen species (ROS) and apoptotic index were investigated in porcine embryos. After in vitro maturation and fertilization, porcine embryos were cultured for 6 days in porcine zygote medium 3 (PZM-3) supplemented with catalpol (0, 100, 200 and $400{\mu}M$, respectively). Blastocyst development not significantly improved in the catalpol treated group when compared with control group. Otherwise, the intracelluar levels of ROS were decreased and the numbers of apoptotic nuclei were reduced in the catalpol ($100{\mu}M$) treated porcine blastocysts (P<0.05). On the other hand, blastocyst development was significantly improved in the catalpol ($100{\mu}M$) treated group when compared with the untreated catalpol group under $H_2O_2$ ($200{\mu}M$) induced oxidative stress (P<0.05). Otherwise, the intracellular levels of ROS in catalpol ($100{\mu}M$) treated group were significantly decreased in the untreated catalpol group under $H_2O_2$ ($200{\mu}M$) induced oxidative stress (P<0.05). Furthermore, the total cell numbers of blastocysts were significantly increased (P<0.05) in the catalpol ($100{\mu}M$) treated group under $H_2O_2$ ($200{\mu}M$) induced oxidative stress, whereas numbers of apoptoic nuclei were significantly reduced (P<0.05). In conclusion, our results indicate that treatment of catalpol may have important implications for improving developmental competence and preimplantation quality of porcine embryos through its anti-oxidant and anti-apoptotic effect.

• Nutritional Sciences
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• 제5권1호
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• pp.26-33
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• 2002

This study was conducted to provide insight into the management and care of the elderly in nursing homes. Eighty-six elderly subjects over 65 years old (38 male, 48 female) in 3 non-fee-paying nursing homes, located in Daejeon and Chungchong areas, were studied. Subjects'dietary intakes by estimated food intake records, and self-perceived health conditions, were studied using questionnaires from July 21 to August 1, 1997. Twenty-one % of the male subjects and 42% of the female subjects were over 80 years. Their predominant past occupation was farming. While 8.3% of the female and no male residents showed a BMI (Body Mass Index) of 27 and above, about 30% of the subjects were underweight and in poor health status in seeing, joint pains, lumbago and shoulder pains. Regarding overall health status, 72.1% of the subjects considered them to be in poor health, and female and male subjects suffered more difficulties from cloudy eyes, joint pains and lumbago and shoulder pains than any other. Neuralgia was the predominant chronic complaint and followed by hypertension in both sexes. Overall, female subjects felt worse off than the male subjects in terms of their health status, that can be attributed to higher average age of the female subjects compared to the male subjects. The elderly were eating a very low fat (about 15 g : 6% of total calorie) diet with low vitamin A and E. Intakes of calories, protein and iron slightly exceeded RDA. The phosphorus intake was more than double the RDA although calcium intake was close to the RDA. From these results, it seems important to doubling the fat intake of the elderly residents in the form of vegetable fat with the object of raising of vitamin E, antioxidant vitamin, and essential fatty acids for the elderly. It is also recommended that the elderly residents should be given adequate calcium and exercise for bone health.

• Journal of Nutrition and Health
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• 제41권4호
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• pp.291-298
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• 2008

To study antioxidant role of zinc, the effects of dietary zinc deficiency and vitamin E supplementation on lipid peroxidation were studied. Levels of zinc and vitamin E in blood and liver were also measured. Forty Sprague-Dawley male rats aging 8 weeks old were used as experimental animals. Zinc deficient diet (Zn, 0 ppm), zinc normal diet (Zn,36.5 ppm), and vitamin E supplemented diet (1,000 IU ${\alpha}$-tocopherol/kg of diet) were used as experimental diet. During the first three weeks, rats were divided into zinc normal (ZnN, 8 animals) and zinc deficient (ZnD, 32 animals) group. Eight rats from each group were sacrificed to get blood and liver after 3 weeks of experiment. The remaining 24 zinc deficient rat were then divided into zinc normal (ZnDN), zinc deficient (ZnDD), vitamin E supplemented (ZnDE) diet groups. After another 3 weeks of experiment, all animals were sacrificed as well. Thiobarbituric acid reactive substanc (TBARS) levels in plasma and liver, conjugated diene levels in liver were measured as lipid peroxidation index. There were no significant differences in food intake, body weight gain, and food efficiency ratio among groups. Weights of liver per 100 g body weight were not significantly different. There were no significant differences in Zn levels in serum. Plasma and liver TBARS level, and liver conjugated diene level were significantly lower in ZnDE than in ZnDN or ZnDD, and significantly higher in ZnDD than in ZnDN. Therefore, it seems that lipid peroxidation is accelerated by dietary zinc deficiency and recovered partly by vitamin E supplementation.