• The Korean Journal of Community Living Science
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• v.15 no.1
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• pp.85-90
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• 2004

To classify the physiological activity by habitat and part of Dandelion (Taraxacum officinale), antioxidative and antimicrobial activities were investigated from methanol extracts of Dandelion. Dandelion was taken from Uiryeong (in Kyungnam province) and from Seoul, and antioxidative and antimicrobial activities were measured for three different parts of the dandelion: the flower, leaf and root. Antioxidative activities of methanol extracts from Dandelion were examined by a DPPH test, and antimicrobial activities were examined against 5 microorganisms: Bacillus subtilis and Bacillus cereus as G(+) microorganisms, and Salmonella typhimurium, Staphylococcus aureus and Eschrichia coli as G(-) microorganisms. No significant differences were found in terms of antioxidative and antimicrobial activities between the dandelions in Uiryeong and the dandelions in Seoul. Antioxidative activity, however, was higher in the flower of the dandelion, irrespective of habitat. Further research with other physiological factors (besides antioxidative and antimicrobial activities) is necessary to shed light on dandelion physiological function and to enhance the utilization of dandelion.

• The Korean Journal of Community Living Science
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• v.15 no.2
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• pp.101-107
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• 2004

Many studies have presented results about the antioxidative and antimicrobial activities of Dandelions(Taraxacum officinale). There has yet to be a study which makes comparisons based on the variety or parts of the Dandelion. To compare the physiological activity, antioxidative and antimicrobial activities were measured by using methanol extracts of Korean and Western Dandelions. The Dandelions were taken from Uiryeong in Kyungnam province, and antioxidative and antimicrobial activities of the flower, leaf and root were measured. Antioxidative activities of the methanol extracts were examined with a DPPH test. Antimicrobial activities were examined against 5 microorganisms. Bacillus subtilis and Bacillus cereus served as G(+) microorganisms, while Salmonella typhimurium, Staphylococcus aureus, and Eschrichia coli served as G(-) microorganisms. There was no significant difference in the antioxidative activity of the methanol extracts from leaves and roots between varieties. The antioxidative activity of the flower extracts was higher in the Korean than in the Western samples but the highest level of antioxidant was lower than 70%. No significant difference in antimicrobial activities between the varieties was observed. More research using physiological factors other than antioxidative and antimicrobial activities is needed to identify physiological functions and enhance the utilization of Dandelions.

• Food Science and Preservation
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• v.6 no.1
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• pp.99-103
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• 1999

Corni Fructus extracts were investigated for antimicrobial and antioxidative activities. In the methanol, ethanol, water extracts from Corni Fructus, antimicrobial activity of the water extract was stronger than the others. Antimicrobial activities were strong against microorganisms such as Streptococcus mutans, Bacillus subtilis, Pseudomonas aeruginosa, Salmonella typhimurium, Escherichia coli and Escherichia coli O-157, the activity against Escherichia coli O-157 was the strongest of all the microorganisms. The growth of all the microorganisms were completely inhibited to 48 hours in the 150${\mu}\ell$ per $m\ell$ of culture media. the shape of E. coli O-157 treated with Corni Fructus water extract was expended and its cellular surface was collapsed severely. When Corni Fructus water extract of 200${\mu}\ell$ was treated, hydrogen donating effect was more than 90%, and antioxidative activity using linoleic acid was similar to 0.1% BHT at the concentration of 50${\mu}\ell$.

• KSBB Journal
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• v.19 no.5
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• pp.381-384
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• 2004

The antimicrobial and antioxidative activities were investigated to confirm the utility of wood vinegar. Antimicrobial activity was performed by paper disc method and liquid culture. The growth inhibition was observed in all microbial species at a dose of as low as 25 $\mu$m of wood vinegar by paper disc method. Also, in liquid culture, S. cerevisiae and P. aeruginosa were more inhibited the growth than others in the concentration of $2\%$ (v/v). For measuring of antioxidative activity, wood vinegar was fractionated with acidic, phenolic, basic and neutral fraction; and their antioxidant activities were measured by the radical scavenging effect on DPPH radical. In four fractions, phenolic fraction showed high antioxidative activity.

• Journal of Life Science
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• v.10 no.5
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• pp.524-528
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• 2000

Antimicrobial and antioxidative effects of Alismatis Rhizoma which has been used as a galenic for diuretic and antiphlogistic were investigated. In a paper disc test, water extract of Alismatis Rhizoma showed antimicrobial activities against almost all of the microbial species tested, while their activities of methanol and ethanol extract was low than the activity of water extract, and the antimicrobial activity of water extract was most prominent against Vibrio parahaemolyticus. Antioxidative activity against linoleic acid was not observed in methanol extract. In contrast to methanol and ethnol and ethanol extract, water extract showed a antioxidative activity against linoleic acid. However, the antioxidative of water extract was lower than that of 0.1% BHT.

• Natural Product Sciences
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• v.12 no.4
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• pp.226-231
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• 2006

Artemisia vulgaris, one of the most religious plants in Nepal, is used in the treatment of various ailments. In this study, antioxidative activity of essential oil from A. vulgaris was evaluated, using different in vitro methods and antimicrobial activity by disc diffusion method against skin disease microorganisms such as Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Candida albicans and Propionibacterium acnes. The essential oil exhibited a concentration-dependent antioxidant activity. It showed strong metal chelating activity with low reducing and antioxidant power. However, gradual increase in radical scavenging activity was obtained with increasing concentration and reaction time. It also possessed a broad spectrum of antimicrobial activity and notable susceptibility was observed against S. pyogenes and P. acnes.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2003.10b
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• pp.44-44
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• 2003

Leaves from natural populations of Artemisia princeps var. orientalis in Suncheon of South Korea were examined for antimicrobial and antioxidative activities monthly (April through October). The antimicrobial activity of the ethyl acetate and water fractions of crude methanol extract from the plant collected monthly against three gram-positive, two gram-negative and one lactic acid bacteria were studied. The ethyl acetate and water fractions of crude methanol extract from A. princeps var. orientalis collected in August and September had the highest antimicrobial activity. Minimum inhibitory concentrations (MIC) for each strain by ethyl acetate fraction of methanol extract from A. princeps var. orientalis was different depending on strains and sampling months. MIC for each strain was the highest in the sample of April, followed by October. In specific, MIC for Bacillus cereus was around 0.25mg/disc in the sample of April. The MIC for Staphyllococcus aureus was 0.01mg/disc in the sample of July, August, and September. It was the lowest value among tested strains and samples. And the antimicrobial activity for Lactobacillus plantarum was not found at any concentrations and sampling months. The in vitro antioxidative activities of methanol extract from Artemisia princeps var. orientalis collected monthly were determined by the scavenging of DPPH radical and inhibition of xanthine oxidase activity. The DPPH scavenging activity of the extracts from the plant sampled in May, June and July were higher than any other months. The xanthine oxidase activity of the extract of A. princeps var. orientalis collected in April and May showed the greatest activity, it is different with antimicrobial activity.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.2
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• pp.338-343
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• 2001

The ethanol extract of Quercus mongolica leaf was fractionated by various organic solvents, and their antimicrobial and antioxidative activities were investigated against several microorganisms. The ethanol extract of Quercus mongolica leaf at two thousand $\mu\textrm{g}$ per disc showed 17~21mm inhibition zone against Gram postive and Gram negative bacteria. Among the various solvent fractions from ethanol extract of Quercus mongolica leaf, the hexane fraction showed the strongest antimicrobial activity. Minimal inhibitory concentration (MIC) of hexane fraction was 250$\mu\textrm{g}$/mL against Bacillus cereus, 250~500$\mu\textrm{g}$/mL against Listeria monocytogenes, 500$\mu\textrm{g}$/mL against Staphylococcus aureus, Salmonella typhimurium, Escherichia coli O157, and Pseudomonas aeruginosa. Also, the hexane and chloroform fraction had the similar antioxidative activity compared to that of butylated hydroxy toluene(BHT).

• The Korea Journal of Herbology
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• v.28 no.3
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• pp.45-51
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• 2013

Objectives : This study was conducted to measure the antioxidative and antimicrobial activities of water- and ethanol-extracts from Betula platyphylla var. japonica, Punica granatum and Rhus javanica against various species of anaerobic bacteria. Methods : In order to evaluate the antioxidative and antimicrobial activities of water- and ethanol-extracts, DPPH radical scavenging and superoxide dismutase (SOD)-like activities were measured in various species such as Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Propionibacterium acnes that induce skin inflammation. Also the total amount of phenol in each water- and ethanol-extract was measured to identify its role in the antioxidative and antimicrobial activities of water- and ethanol-extracts from Betula platyphylla var. japonica, Punica granatum and Rhus javanica. The antibacterial activity of the extracts was measured by confirming the lowest concentrations in disk diffusion and minimum inhibitory concentration (MIC) tests. Results : It was observed that RJE (Rhus javanica-EtOH extracts) show the highest content of total phenol. In addition, for RJE the total phenol content was higher from samples taken from domestic sources than from samples taken from foreign sources. DPPH radical scavenging activity was increased by treatment with PGE (Punica granatum-EtOH extracts), RJE and RJW (Rhus javanica-water extracts). It was observed that SOD-like activity was highest in the treatment with PGE. All of the extracts showed antimicrobial activity on S. epidermidis, S. aureus, P. acnes and E. coli, including those from Punica granatum, and it was noted that the activity was higher with RJE than with RJW. Conclusions : These results provide evidence that ethanol extracts of Punica granatum and Rhus javanica may have a beneficial role as antioxidants and antibiotics. Extracts from domestic samples of Betula platyphylla var japonica appeared to have a greater efficacy than extracts from foreign samples.

• Food Science and Preservation
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• v.6 no.3
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• pp.345-359
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• 1999

The antimicrobial and antioxidative effects of Opuntia ficus-indica vur. saboten (OP) extract with different solvents and extraction methods were investigated. In general, antimicrobial activity of OP was higher in extracts by reflux than in extracts by shake. However, no significant difference in antimicrobial activity was observed between methanol, ethanol and water extract. The antimicrobial activity was significant against all microbes tested, especially against Pseudomonas aeruginosa by shake extract and against Escherichia coli O-157 by reflux extract. A severe morphological change with destruction of outer layer was observed in Salmonella enteritidis treated with water extract. Though OP extract in hydrogen activity was lower than 0.1% BHT, the activity of OP extract showed very high effectivenss than the activity of control. OP extract showed a similar extent of antioxidative activity with BHT.