• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.5
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• pp.407-412
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• 2003

In order to measure an antifreezing tolerance, antifreeze proteins accumulated upon cold acclimation in apoplast were analyzed. As Dongborilho were cold-acclimated for 3 to 74 days there was an abrupt increase in apoplastic proteins up to 30 days and then decrease to the similar levels. Among the known antifreeze proteins, CLP produced in E. coli. and TLP purified from apoplast were used to generate antisera that allow to measure and localize the proteins in leaves of barley. The CLP of 27.7 kDa and TLPs of 6, 26, 27, 30, and 31 kDa were increased in their amounts in apoplast as cold treatment being longer. There was a correlation among the amounts of those proteins accumulated in apoplast and freezing tolerance as shown in field and ion leakage tests for five cultivars. The deposit of CLP was localized in the marginal area and the area adjacent to leaf vescular bundle cells in an increasing manner according to duration of cold acclimation but no variation was observed in terms of it's distribution. Based on the close correlation between levels of antifreeze proteins and degrees of freezing tolerance, the immunological methods was to develop to estimate a freezing tolerance in barley.

• Journal of the Korea institute for structural maintenance and inspection
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• v.21 no.5
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• pp.74-82
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• 2017

Localized corrosions damages in their structural sections can be occurred affected by installed environment conditions with high temperature as near the coastline and humidity or their poor maintenance situation. In bearing supports of steel bridges, especially, lower web and vertical stiffener in end girder support can be easily corroded because of relatively higher humidity due to the narrow space in the end of girder and the wetted accumulated sediments affected by rain water or antifreezing admixture leaked from expansion joint. It can be related to change in their structural performance. In this study, thus, bearing strength test specimens were fabricated considering corrosion damage in the web and vertical stiffeners and the change in their bearing strengths were experimentally evaluated. From the test results, localized corrosion damage of structural members in the end girder affected the bearing strength of end girder support, especially, localized corrosion damage of the vertical stiffener relatively highly affected their bearing strengths.

• Korean Journal of Plant Tissue Culture
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• v.22 no.1
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• pp.15-28
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• 1995

In order to identify an antifreeze proteins responsible for freeze-tolerance in barley the proteins accumulated in extracellular space during cold acclimination were extracted and analyzed. After 42 days cold treatment there were several proteins sized of 70, 21, 16, 14 KDa increased in their amount accumulated in extracellular space. In addition, continuously sized polypeptides smaller than 10 KDa were found to be increased in their amount as cold treatment prolongs. Since these proteins were not detectable in total leaf protein extract it appears that the procedure used to isolate extracellular extract was valid. A similarity in profile of the extracellular proteins isolated from barley and rye may indicate a possibility for these proteins to be an antifreeze proteins since the same extract from rye was reported to show an antifreezing activity.

• Korean Journal of Agricultural and Forest Meteorology
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• v.8 no.4
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• pp.270-274
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• 2006

This study proposes a new method for automatic recording of snowfall by a mass unit which is required in weather forecast and hydrology research. In this method the weight of a buoyancy bar submerged in a liquid is measured by a strain-gauge loadcell. Field test results of the strain-gauge loadcell showed good stability as well as high accuracy. Indoor tests of the instrument using a large tank of 120 cm diameter and 25 cm height connected to a small tank measured the liquid level with a good stability, showing a measurement error of less than 0.1 mm in a 100 mm range. This method of water depth measurement is very useful in measuring snowfall because it has no limitation on the funnel size of the instrument. In addition, an antifreezing solution instead of water used in the tank makes a heating system for melting snow unnecessary.

• Korean Journal of Animal Reproduction
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• v.20 no.3
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• pp.233-238
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• 1996

This study was conducted to investigate the effects of cryoprotective agents and thawing temperature on the survival rate of the bisected embryos of mouse. The results of this study were summairzed as follows: 1. In in vitro culture after frozen with DMSO, glycerol, ethylene glycol or propanediol and thawed, the rates of normally developed bisected morula which was denuded were 31.7, 39.1, 28.0 and 23.1%, respectively. 2. The survival rates of bisected morula encased into the zona pellucida in in vitro culture after frozen with DMSO, glycerol, ethylene glycol or propanediol and thawed were 72.4, 68.7, 64.0 and 59.5%, respectively. 3. The survival rates of bisected denuded blastura in in vitro culture after frozen with DMSO, glycerol, ethylene glycol or propanediol and thawed were 48.3, 44.8, 32.1 and 28.6%, respectively. 4. The survival rates of bisected blasturae encased into the zona pellucida in in vitro culture after frozen with DMSO, glycerol, ethylene glycol or propanediol and thawed were 73.6, 67.4, 53.0 and 49.1%, respectively. 5. In in vitro culture after frozen with DMSO, glycerol, ethylene glycol or propanediol and thawed at room temperature, the rates of the normally developed bisected morula which was encased into the zona pellucida were 67.1, 62.3, 57.7 and 53.0%, respectively, and those of the bisected blasturae encased into the zona pellucida were 70.8, 65.4, 56.6 and 52.1%, respectively. 6. The survival rates of bisected morula which was encased into the zona pellucida in in vitro culture after forzen with DMSO, glycerol, ethylene glycerol or propanediol and thawed at 37$^{\circ}C$ were 74.3, 71.3, 63.9 and 57.4%, respectively, and those of bisected blastura encased into zona pellucida were 76.0, 69.1, 61.1 and 56.1%, respectively.

• 한국신재생에너지학회:학술대회논문집
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• 2010.06a
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• pp.200.2-200.2
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• 2010

This study was carried out in order to reduce the amount of underground water which is used in the water curtain system for retaining heat. To proceed to the research, two plastic green houses of water curtain system were installed. One was equipped of internal small tunnel for keeping warm air in the interior of the house. Then the internal small tunnel for keeping warm air was fitted with PVC duct of 50cm in diameter filled with subsurface water. Storing surplus solar energy in the water filled in PVC duct was the method used to this house. Another was installed with FCU in the middle of the house, and was fitted a circulation motor in water tank for heat storage which was operated from 10 a.m. to 4 p.m. in order to interchange heat with FCU. The latter was installed with four FCUs which has a capacity of 8000kcal per hour. Consequently about 5 degrees celsius could be maintained in the interior of the internal small tunnel for keeping warm air with the external temperature of more than minus 5 degrees celsius. It appeared that the alteration of an internal temperature of the house was flexible depending on the sunlight during daytime. It happened that to prevent the water from freezing, mixing antifreezing liquid in the flowing water of FCU or changing the operating method of FCU was a suitable measure. Also, in order to use the surplus solar thermal energy on plastic green house of water curtain system efficiently, storing the surplus heat during daytime simultaneously finding a method of using water curtain systematic underground water happened to be important. As a result of this research, when the house's interior temperature is below zero the operation of FCU appeared to be impossible. Therefore when supposed that the amount of water used in the house is 150~200ton for stable operation of FCU, using the system mentioned in the above research happened to be appropriate of reducing the amount of subsurface water from 80% to 100% when maintaining the interior of internal small tunnel's temperature for keeping warm air of 5 degrees celsius at the extreme temperature of minus 5 degrees celsius.

• International Journal of Highway Engineering
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• v.12 no.2
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• pp.99-106
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• 2010

Until now, the thickness design of anti-freezing layer has been empirically conducted using the frost depth determined from the freezing index. This approach cannot consider the structural properties of anti-freezing layer, which can cause the over-design of pavement structure. This paper presents results of structural evaluation of anti-freezing layer using the Falling Weight Deflectormeter (FWD) deflections. The FWD testing was directly conducted on top of the subbase layer located at the embankment, cutting, and boundary area of each section. It is observed from this testing that the center deflections of pavement structure with anti-freezing layer are smaller than those without anti-freezing layer. The deflection reduction rates are 15~55% in the embankment, 11~64% in the cutting, and 2~38% in the boundary, respectively. It was also found that the use of antifree zing layer enables to reduce the Surface Curvature Index (SCI) values up to 24 percent. Fatigue lives show that pavement structure with antifreezing layer are about two times higher than the those without anti-freezing layer. This fact indicates that the anti-freezing layer should be considered as a structural layer in the asphalt pavement system.

• Journal of Biosystems Engineering
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• v.36 no.3
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• pp.217-222
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• 2011

This study was carried out in order to reduce the amount of underground water which is used in the double layered single span plastic greenhouse for retaining heat. For this research, two plastic green houses of the double layered single span plastic greenhouse were installed. There was equipped of internal small tunnel for keeping warm air in the interior of the house. Then the internal small tunnel for keeping warm air was fitted with PVC duct of 50 cm in diameter filled with subsurface water. The surplus solar energy in the greenhouse was stored in the water in the PVC duct. Four FCUs (Fan Coil Unit), which has the capacity of 8,000 kcal per hour, were installed in the middle of the house, and a circulation motor in heat storage water tank was operated from 10:30 a.m. to 16:00 p.m. in order to circulate water between the water tank and the FCUs. Consequently about 5 degrees celsius could be maintained in the interior of the internal small tunnel for keeping warm air with the external temperature of lower than minus 5 degrees celsius. It appeared that the alteration of an internal temperature of the house was flexible depending on the sunlight during daytime. To prevent the water freezing, mixing antifreezing liquid in the water or operating FCU continuously was needed. Also, in order to use the surplus solar thermal energy on plastic green house of water curtain system efficiently, storing the surplus heat during daytime simultaneously finding a method of using water curtain systematic underground water happened to be important. As a result of this research, when the house's interior temperature is below zero the operation of FCU appeared to be impossible. Considering the amount of water used in the house with water-curtain-heating system is 150~200 ton per day, using the system mentioned in this research showed that reducing the underground water more than 80% in order to maintain the internal temperature as the level of 5 degree celsius at the extreme temperature of minus 5 degrees celsius.