• Title/Summary/Keyword: anticariogenic effects

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Anticariogenic Effects and Inhibition of Glucosyltransferase Activity of Chrysanthemun indicum L. Extracts (감국(Chrysanthemun indicum L.) 추출물의 항충치효과와 Glucosyltransferase 저해활성 탐색)

  • Kim, Seon-Jae;Park, Yun-Mi;Jung, Soon-Teck
    • Journal of the Korean Society of Food Culture
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    • v.20 no.3
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    • pp.341-345
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    • 2005
  • The anticariogenic effects and inhibition of glucosyltransferase activity of Chrysanthemun indicum L. extracts were investigated against 5 strains of microorganisms. The ethanol extracts of Chrysanthemun indicum L. showed the growth inhibition effects on the Streptococcus mutans and oral bacteria. The inhibition rate of glucosyltransferase activity of Chrysanthemun indicum L. extracts showed 78.4 to 92.3% range. The extracts were analysed by using solvent fractionation, silica gel adsorption chromatography, Sephadex LH-20 column chromatography, TLC, HPLC techniques. Three components whose molecular weights ranged from 200 to 400 were confirmed to have the anticariogenic activity.

Anticariogenic Activity and Glucosyltransferase Inhibitory Effects of Extracts from Pine Needle and Twig (솔잎 및 소나무 가지 추출물의 항충치 활성 및 glucosyltransferase 억제 효과)

  • Choi, Hee-Don;Koh, Yoon-Jeoung;Choi, In-Wook;Kim, Yun-Sook;Park, Yong-Kon
    • Korean Journal of Food Science and Technology
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    • v.39 no.3
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    • pp.336-341
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    • 2007
  • To investigate anticariogenic effects of extracts from pine needles and twigs, we measured the extracts' antimicrobial activities against Streptococcus mutans, their glucosyltransferase (GTase) inhibitory effects and their stabilities under various conditions of heat and pH. The 70% ethanol extracts of pine needles and twigs showed greater antimicrobial activities against S. mutans than the hot water extracts, and the pine twig extracts showed greater antimicrobial activity than the pine needle extracts. More specifically, the n-hexane fractions of the pine needles and twigs had the greatest effects against S. mutans, and their minimal inhibition concentration (MIC) was 0.6 mg/mL. The results of the extracts' GTase inhibitory effects indicated that the 70% ethanol extracts had greater effects than hot water extracts, and the pine twig extracts showed greater effects than the pine needle extracts. The antimicrobial activity against S. mutans for the pine needle n-hexane traction was greatly reduced as temperature increased, and was also largely affected by changes in pH. These results suggest that extracts from pine needles and twigs have anticariogenic effects and could be used as anticariogenic food additives.

Antioxidant and anticariogenic activities of enzymatic hydrolysate from spent coffee grounds (커피박 효소분해물의 항산화 및 항충치균 활성)

  • Man-Jin In;Yu Min Jang;Min Young Jo;Hee Jeong Kim;Dong Chung Kim
    • Journal of Applied Biological Chemistry
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    • v.66
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    • pp.462-466
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    • 2023
  • After treating spent coffee grounds with alkali, extracts were prepared by using Viscozyme and Alcalase, respectively. Treatment of spent coffee grounds with alkali and enzymes increased the content of phenolic compounds in the extracts, thus possessing the good scavenging activities on free and cation radicals. In particular, the extract obtained by continuous treatment with alkali and Alcalase on spent coffee grounds had the best content of phenolic compounds and antioxidant activity, and inhibited the growth of Streptococcus mutans in proportion to the concentration. In conclusion, the Alcalase-enzymatic hydrolysate of alkali-treated spent coffee grounds showed excellent antioxidant and anticariogenic effects.

Anticariogenic effects of Coptis chinensis Franch Extract (황련(Coptis chinensis Franch) 추출물의 항충치효과)

  • Jang, Gui-Hyun;Ahn, Byung-Young;Oh, Suk-Heung;Choi, Dong-Seong;Kwon, Yong-Ju
    • Korean Journal of Food Science and Technology
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    • v.32 no.6
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    • pp.1396-1402
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    • 2000
  • The effects of Coptis chinensis Franch(CCF) extract on the growth, acid production, cell adherence and glucosyltransferase(GTase) activity of Streptococcus mutans JC-2 were investigated. Methanol extract from CCF showed a strong inhibitory effect on the growth of S. mutans. The minimal inhibition concentration(MIC) of the methanol extract was determined as $130\;{\mu}g/mL$, whereas MIC of water extract was $200\;{\mu}g/mL$. MICs of berberine and palmatine were $50\;{\mu}g/mL$ and $110\;{\mu}g/mL$, respectively, showing stronger antimicrobial activities than the extracts of CCF. Antimicrobial activities of methanol extract, berberine and palmatine were not decreased by heating at $121^{\circ}C$ for 15 min, suggesting that the antimicrobial components including berberine and palmatine are heat-stable. Acid production of S. mutans was decreased by methanol and water extracts, berberine and palmatine. The activity of GTase was inhibited by methanol extract, berberine and palmatine at $300\;{\mu}g/mL$ with 23.2%, 46.1% and 17.1%, respectively, but was not inhibited by water extract. The water extract and palmatine at sub-MICs inhibited the adherence of S. mutans to glass surface by 59.2% and 41.7%, respectively. These results suggest that CCF extracts have anticariogenic effects and could be used as an anticariogenic food additive.

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Anticariogenic Effects of Unripe Apple Extract (애사과 추출물의 충치억제효과)

  • Yoon, Suck-Young;Kim, Sung-Hoon;Chung, Hae-Lim;Lee, Jeong-Jun;Huh, Chul-Sung;Baek, Young-Jin
    • Korean Journal of Food Science and Technology
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    • v.32 no.1
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    • pp.168-173
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    • 2000
  • Anticariogenic activity of the unripe apple extract was studied by observing the inhibitory effects on GTase(glucosyltransferase) activity, cell adherence and acid production of Streptococcus mutans. Among the four S. mutans strains, S. mutans MT 8148 had the highest water-insoluble glucan forming activity. (+)-Catechin and tannic acid, the major components of the unripe apple polyphenols inhibited GTase activity by 60% at 1 mg/ml and 90% at 5 mg/ml. Tannic acid and unripe apple extract inhibited adherence ability of S. mutans by 50% and 30%, respectively. But the acid production of S. mutans was not influenced by the polyphenols. Disc diffusion test showed that the polyphenols have no antimicrobial activity against S. mutans, which indicates that the inhibition of GTase activity and cell adherence were not resulted from the cell growth inhibition. Our results convinced the possible application of the unripe apple extract as the anticariogenic food additives.

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Anti-microbial and Anticariogenic Activity of Yam and Prunella Extract against Oral Microbes (구강병인균에 대한 마와 꿀풀추출물의 항균.항우식효과)

  • Jung, Gi-Ok;Min, Kyung-Jin
    • Journal of Environmental Health Sciences
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    • v.33 no.2 s.95
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    • pp.137-144
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    • 2007
  • Yam, Prunella was stepwise extracted with hexane, chloroform, ethyl acetate, butanol, and water. Anti-microbial activity of each extract was investigated. Hexane extract was tested for anti-microbial effect on Streptocaccus mutans, one of causative factor of dental caries. Methanol extracts of 7 plants were investigated to anti-microbial effects on S. mutans KCTC 5316, P. gingivalis KCTC 5352, S. aureus KCTC 1927 by means of agar diffusion method. Methanol extract of Yam and Prunella revealed anti-microbial activity against S. mutans, P. gingivalis, and S. aureus. Also, hexane fraction of Yam revealed anti-microbial activity against S. mutans. In sequence of hexane, chloroform, ethylacetate, butanol fraction by Prunelia acted as potent anti-microbial agent on P. gingivalis. The measured MIC of hexane fraction of Yam and Prunella on S. mutans KCTC 5316 strain was 0.25 mg/ml and 0.5 mg/ml and the MIC of hexane fraction of Prunella on S. aureus was 0.5 mg/ml. The hexane fraction of Yam and Prunella suppressed viable ceil counts(VCC) of S. mutans, especially after 24 hrs. The Prunella hexane fraction suppressed VCC of S. aureus, after 12 and 24 hrs. Tested concentrations were 0.1, 0.25 and 0.5 mg/ml. the results were compared with control (0 mg/ml). The pH of S. mutans media and GTase activity were determined to evaluate the anticariogenic activity of Yam, Prunella hexane fraction. The pH were increased from 5.6 to 7.0-7.2 in concentration of 2.0 mg/ml. Yam hexane extraction revealed 35% inhibition to GTase activity and Punella inhibited 25% of GTase. These results suggest that the hexane extracts of Yam and prunella have Antibacterial activities against S. mutans, P. gingivalis, S. aureus and have preventive effect on dental caries.

Comparison of Anticariogenic Effect after Applying Fluoride Varnish on Sound and Artificial Caries Enamel (정상법랑질과 인공우식법랑질에 불소바니쉬 도포 후 항우식 효과 비교)

  • Jeong, Moon-Jin;Lim, Ji-Hyang;Min, Ji-Hye;Jeong, Soon-Jeong;Son, Jung-Hui;Lim, Do-Seon
    • Journal of dental hygiene science
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    • v.13 no.4
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    • pp.461-470
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    • 2013
  • In order to examine the anticariogenic effect after fluoride varnish was applied to sound enamel and artificial caries enamel, anterior teeth of healthy cattle were used and divided into four groups such as group 1 (sound enamel), group 2 (application of fluoride varnish to sound enamel), group 3 (artificial caries enamel) and group 4 (application of fluoride varnish to artificial caries enamel). Remineralization on the surface of enamel and changes of crystalline structure after demineralization were observed by using a field emission scanning electron microscope (FE-SEM). Quantitative analysis of Ca and P was measured by using the energy dispersive X-ray spectrophotometer (EDS). The following conclusions were obtained: 1)Surface pattern of enamel was the roughest in group 3 due to the defects caused by porosity and microcracks. Group 4, group 1 and group 2 were followed in order; 2) It was found that pattern of crystalline structures in a group of application of fluoride varnish and a group of no application showed bigger change in artificial caries enamel than that in sound enamel. In other words, groups 4 and 1 showed a similar pattern; 3) The contents of Ca and P were higher in groups of application of fluoride varnish (group 2 and group 4) than in groups of no application of fluoride varnish (group 1 and group 3). Taken results of this study together, in the case of application of fluoride varnish, crystalline structure was changed by remineralization even in the sound enamel. In particular, porous structures showed a smooth and uniform pattern due to the recalcification in the artificial caries enamel. In addition, according to results of EDS analysis, the contents of Ca and P were increased and it had great anticariogenic effects which inhibit decalcification of sound enamel and artificial caries enamel.

Anticariogenic Activity and Glucosyltransferase Inhibition of Phenolic Compounds (페놀성 화합물의 항충치활성 및 Glucosyltransferase 억제효과)

  • Kim, Seon-Jae;Park, In-Bae;Kang, Seong-Gook;Chung, Dong-Ok;Jung, Soon-Teck
    • Journal of the Korean Society of Food Culture
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    • v.20 no.5
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    • pp.603-607
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    • 2005
  • Fourteen phenolic compounds(benzoic acid, p-hydroxybenzoic acid, protocatechuic acid, vanillic acid, syringic acid, gallic acid, caffeic acid, ferulic acid, (+)-catechin, quercetin, rutin, catechol, chlorogenic acid and L-ascorbic acid) were examined for their effects on the anticarigenic activity. Among tested samples, catechol was significantly inhibited the S. mutans, exhibiting an clear zone 18.5-19.5mm by 10 mg/disc level. The minimal inhibition concentration(MIC) of the phenolic compounds for Streptococcus mutans, M1 and M2 strain were determined as 2,000 ppm, whereas catechol was 1,000 ppm. The activity of glucosyltransferase(GTase) was significantly inhibited by catechol, at 10 ppm(58.7%), 50 ppm(60.7%) and 100 ppm(88.4%) and 500 ppm(89.6%), respectively. Among them, catechol showed most significant anticariogenic activity as well as inhibition of GTase activity.

THE ANTICARIOGENIC EFFECTS OF XYLITOL ON MUTANS STREPTOCOCCI IN CHILDREN WITH MIXED DENTITION (혼합치열기 어린이에서 mutans streptococci에 대한 자일리톨 껌의 항균효과에 관한 연구)

  • Park, Jae-Hong
    • Journal of the korean academy of Pediatric Dentistry
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    • v.34 no.4
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    • pp.632-638
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    • 2007
  • The purpose of this study was to investigate the effect of xylitol chewing gums on mutans streptococci (MS) counts in saliva. Sixty two children 6 to 11 years old were randomly assigned into one of three groups. Stimulated saliva specimens were plated in duplicate on conventional selective culture (mitis salivarius kanamycin bacitracin agar) for mutans streptococci. Polymerase chain reaction (PCR) amplication was performed to identify MS. After the 4-month period a significant decrease of the MS counts occurred in the group B (two gum 3 times a day; P < 0.05) but not in group A (one gum 3 times a day) and control group (didn't receive xylitol gum). According to qualitative evaluations, xylitol use did reduce the levels of MS in mixed dentition children. It has been suggested that a daily intake of 2 tablet for 3 times a day (about 10g) is needed in order to obtain a clinical anticariogenic effect.

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Anticariogenic Activities of Lactobacillus sakei K-7 Isolated from Kimchi (김치로부터 분리한 Lactobacillus sakei K-7의 항충치 활성 특성)

  • Moon, Jin-Seok;Ahn, Ji-Eun;Han, A-Reum;Heo, Jeong-Seon;Eom, Hyun-Ju;Shin, Chul-Soo;Choi, Hye-Sun;Han, Nam-Soo
    • KSBB Journal
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    • v.26 no.6
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    • pp.513-516
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    • 2011
  • The occurrence of dental caries is mainly associated with oral pathogens, especially cariogenic Streptococcus mutans. The aim of this study was to isolate and characterize lactic acid bacterium showing inhibitory activity against cariogenic Streptococcus mutans. As results, an isolate with strong inhibitory activity was obtained from Kimchi and it was identified as Lactobacillus sakei by API and 16S rRNA gene analyses. This strain secreted an inhibitory compound in cell growth medium and the activity of the compound was completely disappeared by proteinase K revealing the fact that the compound is proteinous substance, bacteriocin. Optimal culture condition for bacteriocin production by Lb. sakei K-7 was at pH 7.5 and $37^{\circ}C$ for 18 h. Oral administration of this isolate may give anticariogenic and probiotic effects on hosts.