• Korean Journal of Plant Resources
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• v.21 no.6
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• pp.431-434
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• 2008

Dental caries is the destruction of the enamel of teeth by Streptoccus mutans. S. mutans has been isolated from human dental plaque and is associated with the initial development of enamel lesions. We have studied the antibacterial action of the fruit of Callistemon citrin us against a cariogenic bacterium, S. mutans. From the fruit of C. citrinus, piceatannol (3,3',4',5-tetrahydroxystilbene) was isolated by repeated column chromatography with $SiO_2$ and Sephadex LH-20. Its structure was elucidated by instrumental analysis using 1D-NMR, 2D-NMR and EI-MS. This compound was isolated from the fruit of C. citrin us for the first time. The anticarcinogenic activity of this compound was determined by using agar well-diffusion method and minimal inhibition concentration (MIC).

• Journal of Environmental Health Sciences
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• v.33 no.2 s.95
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• pp.137-144
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• 2007

Yam, Prunella was stepwise extracted with hexane, chloroform, ethyl acetate, butanol, and water. Anti-microbial activity of each extract was investigated. Hexane extract was tested for anti-microbial effect on Streptocaccus mutans, one of causative factor of dental caries. Methanol extracts of 7 plants were investigated to anti-microbial effects on S. mutans KCTC 5316, P. gingivalis KCTC 5352, S. aureus KCTC 1927 by means of agar diffusion method. Methanol extract of Yam and Prunella revealed anti-microbial activity against S. mutans, P. gingivalis, and S. aureus. Also, hexane fraction of Yam revealed anti-microbial activity against S. mutans. In sequence of hexane, chloroform, ethylacetate, butanol fraction by Prunelia acted as potent anti-microbial agent on P. gingivalis. The measured MIC of hexane fraction of Yam and Prunella on S. mutans KCTC 5316 strain was 0.25 mg/ml and 0.5 mg/ml and the MIC of hexane fraction of Prunella on S. aureus was 0.5 mg/ml. The hexane fraction of Yam and Prunella suppressed viable ceil counts(VCC) of S. mutans, especially after 24 hrs. The Prunella hexane fraction suppressed VCC of S. aureus, after 12 and 24 hrs. Tested concentrations were 0.1, 0.25 and 0.5 mg/ml. the results were compared with control (0 mg/ml). The pH of S. mutans media and GTase activity were determined to evaluate the anticariogenic activity of Yam, Prunella hexane fraction. The pH were increased from 5.6 to 7.0-7.2 in concentration of 2.0 mg/ml. Yam hexane extraction revealed 35% inhibition to GTase activity and Punella inhibited 25% of GTase. These results suggest that the hexane extracts of Yam and prunella have Antibacterial activities against S. mutans, P. gingivalis, S. aureus and have preventive effect on dental caries.

• Journal of the korean academy of Pediatric Dentistry
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• v.25 no.1
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• pp.62-75
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• 1998

The main purpose of this study was to compare the anticariogenecity of glass ionomer restorative material polymerized by argon laser versus visible light, The results from the present study can be summarized as follows; 1. Under the polarized light microscope, the specimens of laser-cured group showed the shallower lesion body than that of visible-light cured group, both in the stage of lesion initiation and progression. 2. Glass ionomer material cured by visible light showed shallower body of lesion than that of composite resin cured by argon laser at the stage of lesion progression(p<0.05). It was suggested fluoride released from the glass ionomer might have the additive anticariogenic effect. 3. Statistical difference between groups on depth of lesion body was evident after lesion progression (p<0.05). It was suggested that anticariogenic effect by argon laser was more effective at the stage of lesion progression than the lesion initiation. 4. The increment of lesion body during progression was highest in group IV (p<0.05). 5. Based upon the above-mentioned results of this study, it can be concluded that the advantage of anticariogenic effect and short curing time of argon laser in glass ionomer polymerization should be considered in children and adolescents whose caries activity is relatively higher.

• Journal of Pharmacopuncture
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• v.26 no.1
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• pp.44-52
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• 2023

Objectives: Dental caries is one of the most prevalent human diseases worldwide. The disease initiates with bacterial adherence to the tooth surface followed by the formation of dental plaques. Mutans streptococci and Candida albicans are principal oral microorganisms involved in the initiation and development of dental caries. Phytochemicals have been shown to possess promising antimicrobial properties against a wide range of microorganisms and can be used for the prevention and treatment of dental caries. Herein, we reviewed literature on plants that are traditionally used for their antimicrobial properties or possess promising anticariogenic activity. We selected aerial parts of Scrophularia striata (S. striata) and galls of Quercus infectoria (Q. infectoria) and investigated their antimicrobial activity against cariogenic microorganisms. Methods: Water soluble fractions were obtained from hydroalcoholic extracts of S. striata and Q. infectoria and their antimicrobial activity against Streptococcus mutans (S. mutans), Streptococcus sobrinus (S. sobrinus), and Candida albicans (C. albicans) was evaluated separately and in combination. The extracts were then used for preparing an herbal mouthwash whose stability and tannic acid content were evaluated over 60 days. Results: Q. infectoria gall extract possesses efficient antimicrobial activity that was synergistically enhanced in the presence of S. striata extract. Mouthwash prepared using these extracts showed desirable organoleptic characteristics, antimicrobial activity, and stability. Conclusion: Extracts of S. striata and Q. infectoria galls can be used together for preparing dental products with effective anticariogenic properties. Our study highlights the importance of extensive pharmacological investigations when using herbal products alone or in combination with other chemical substances.

• Journal of the korean academy of Pediatric Dentistry
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• v.24 no.1
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• pp.148-172
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• 1997

Sixty human premolar teeth were used for this in vitro study. After each tooth was sectioned mesiodistally, one half was used for the experimental group and the other half for the control. Three groups were made for each fluoride applying method and twenthy teeth were assigned to each group. Ten teeth were used for evaluating total fluoride amount and the other ten were used for firmly-bound fluoride. Fluorshield was used for fluoride-releasing sealant and 1.23% APF, 0.05% NaF were used for topical application fluorides. Each tooth was cleaned with a tooth brush using nonfluoride containing pumice before the experiment. In the sealant group, fluoroshield was applied to the enamel surface without etching procedure and stored in $37^{\circ}C$ saline for 30 days. After 30 days, sealant was removed with explorer without scratching the enamel surface and washed with distilled water and dried. In the APF group, each tooth was immersed in 1.23% APF for 30 min then washed and dried in the same manner. In the NaF group, each tooth was immersed in 0.05% NaF for 24 hours then washed and dried as described above. After each fluoride regimen was applied, ten teeth were randomly selected from each group and immersed in 1M KOH solution for 24 hours to remove loosely-bound fluoride possibly deposited by the three different fluorides applied. In each group, total fluoride amount deposited and the amount of enamel removed by acid biopsy were calculated. After loosely-bound fluoride was removed, firmly-bound fluoride deposited and the amount of enamel removed by acid biopsy were also calculated. Total fluoride amount deposition was significantly increased in the APF and NaF groups, but not in the sealant group. Amount of enamel removed by acid-biopsy was also significantly diminished in the APF and NaF groups, but not in the sealant groups. After loosely-bound fluoride was removed from each groups, no statistical difference was found in the amount of firmly-bound fluoride in any groups. Also no effect of firmly-bound fluoride on enamel dissolution was shown in any groups after loosely-bound fluoride was removed from each group. In conclusion, topical application method of APF or NaF is more effective than fluoride-releasing sealant application to make $CaF_2$ coating on enamel surface and $CaF_2$ coating is the main source for anticariogenic effect of fluoride. However, longterm anticariogenic effect of fluoride-releasing sealant should be further evaluated.

• Journal of dental hygiene science
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• v.13 no.4
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• pp.461-470
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• 2013

In order to examine the anticariogenic effect after fluoride varnish was applied to sound enamel and artificial caries enamel, anterior teeth of healthy cattle were used and divided into four groups such as group 1 (sound enamel), group 2 (application of fluoride varnish to sound enamel), group 3 (artificial caries enamel) and group 4 (application of fluoride varnish to artificial caries enamel). Remineralization on the surface of enamel and changes of crystalline structure after demineralization were observed by using a field emission scanning electron microscope (FE-SEM). Quantitative analysis of Ca and P was measured by using the energy dispersive X-ray spectrophotometer (EDS). The following conclusions were obtained: 1)Surface pattern of enamel was the roughest in group 3 due to the defects caused by porosity and microcracks. Group 4, group 1 and group 2 were followed in order; 2) It was found that pattern of crystalline structures in a group of application of fluoride varnish and a group of no application showed bigger change in artificial caries enamel than that in sound enamel. In other words, groups 4 and 1 showed a similar pattern; 3) The contents of Ca and P were higher in groups of application of fluoride varnish (group 2 and group 4) than in groups of no application of fluoride varnish (group 1 and group 3). Taken results of this study together, in the case of application of fluoride varnish, crystalline structure was changed by remineralization even in the sound enamel. In particular, porous structures showed a smooth and uniform pattern due to the recalcification in the artificial caries enamel. In addition, according to results of EDS analysis, the contents of Ca and P were increased and it had great anticariogenic effects which inhibit decalcification of sound enamel and artificial caries enamel.

• Journal of the korean academy of Pediatric Dentistry
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• v.29 no.1
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• pp.1-10
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• 2002

The purpose of this study was to evaluate the fluoride release and anticariogenic effect of two compomers which is known to have been developed to improve the weak properties of glass ionomer cement and composite resin. Z-100 as composite resin(Group I), Dyract AP(Group II) and F-2000(Group III) as compomer, and Fuji II LC as glass ionomer cement(Group IV) were used as test materials and evaluations were peformed by pH/ISE meter far analyses of fluoride and polarizing microscope for analyses of anticariogenicity. The results can be summarized as follows: 1. The amount of fluoride release in compoite resin and compomer groups showed general pattern of decline during test period. Z-100 showed no fluoride release during test period. Fuji II LC showed the highest fluoride release among test groups and then F-2000 were followed. 2. The least resistance to dimineralization was observed microscopically in Z-100 group which has no fluoride in it. The best resistance to dimineralization was observed microscopically in Fuji II LC group and then compomer groups were followed. 3. Significant difference in lesion area was found between Fuji II LC group and another groups. Significant difference in lesion area was found between compomer groups and Z-100 group. No significant difference in lesion area was found between Dyract AP group and F-2000 cup. 4. Two compomers showed continual fluoride release and anticariogenic effect around filling materials. therefore, compomer was evaluated very attractive restorative material in pediatric dentistry.

• Korean Journal of Food Science and Technology
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• v.32 no.1
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• pp.168-173
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• 2000

Anticariogenic activity of the unripe apple extract was studied by observing the inhibitory effects on GTase(glucosyltransferase) activity, cell adherence and acid production of Streptococcus mutans. Among the four S. mutans strains, S. mutans MT 8148 had the highest water-insoluble glucan forming activity. (+)-Catechin and tannic acid, the major components of the unripe apple polyphenols inhibited GTase activity by 60% at 1 mg/ml and 90% at 5 mg/ml. Tannic acid and unripe apple extract inhibited adherence ability of S. mutans by 50% and 30%, respectively. But the acid production of S. mutans was not influenced by the polyphenols. Disc diffusion test showed that the polyphenols have no antimicrobial activity against S. mutans, which indicates that the inhibition of GTase activity and cell adherence were not resulted from the cell growth inhibition. Our results convinced the possible application of the unripe apple extract as the anticariogenic food additives.

• Korean Journal of Food Science and Technology
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• v.32 no.6
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• pp.1396-1402
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• 2000

The effects of Coptis chinensis Franch(CCF) extract on the growth, acid production, cell adherence and glucosyltransferase(GTase) activity of Streptococcus mutans JC-2 were investigated. Methanol extract from CCF showed a strong inhibitory effect on the growth of S. mutans. The minimal inhibition concentration(MIC) of the methanol extract was determined as $130\;{\mu}g/mL$, whereas MIC of water extract was $200\;{\mu}g/mL$. MICs of berberine and palmatine were $50\;{\mu}g/mL$ and $110\;{\mu}g/mL$, respectively, showing stronger antimicrobial activities than the extracts of CCF. Antimicrobial activities of methanol extract, berberine and palmatine were not decreased by heating at $121^{\circ}C$ for 15 min, suggesting that the antimicrobial components including berberine and palmatine are heat-stable. Acid production of S. mutans was decreased by methanol and water extracts, berberine and palmatine. The activity of GTase was inhibited by methanol extract, berberine and palmatine at $300\;{\mu}g/mL$ with 23.2%, 46.1% and 17.1%, respectively, but was not inhibited by water extract. The water extract and palmatine at sub-MICs inhibited the adherence of S. mutans to glass surface by 59.2% and 41.7%, respectively. These results suggest that CCF extracts have anticariogenic effects and could be used as an anticariogenic food additive.

• Proceedings of the KSCN Conference
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• 2005.10a
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• pp.84-84
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• 2005