• International Journal of Oral Biology
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• 제30권2호
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• pp.59-63
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• 2005

Postantibiotic effects (PAE) refer to suppression of the bacterial growth following limited periods of exposure to an antibiotic and subsequent to the removal of the antibiotic agent. Fusobacterium nucleatum and Porphyromonas gingivalis are Gram-negative anaerobic bacteria associated with several periodontal diseases. In this study, postantibiotic effects (PAE), postantibiotic sub-MIC effect (PA SME) and sub-MIC effect (SME) of antibiotics on F. nucleatum ATCC 25586 and P. gingivalis W50 were investigated. The PAE was induced by 10X the MIC of antibiotic and antibiotic was eliminated by washing. The PA SMEs were studied by addition of 0.1, 0.2 and 0.3X MICs during the postantibiotic phase of the bacteria, and the SMEs were studied by exposition of the bacteria to antibiotic at the sub-MICs only. Amoxicillin, doxycycline and tetracycline induced PAE for F. nucleatum ATCC 25586 and P. gingivalis W50. But metronidazole and penicillin induced PAE for only F. nucleatum ATCC 25586. Metronidazole and doxycycline induced PA SME and SME for both species of anaerobic bacteria used in this study. The PA SME values for both strains were substantially longer than the SME values. The present study showed the existence of PAE, PA SME and SME for various antibiotics against F. nucleatum ATCC 25586 and P. gingivalis W50.

• 한국습지학회지
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• 제13권3호
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• pp.697-705
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• 2011

본 연구의 목적은 다제 항생제 내성특성을 가진 pB10을 함유한 Escherichia coli DH 5 alpha,(E.coli $DH5{\alpha}$)를 대상 미생물로 하여 염소와 오존의 살균효율을 비교하는 것이다. 또한 다제 내성플라스미드 pB10에 대한 염소와 오존에 의한 제거율을 조사하였다. 주입농도 대비 오존살균이 염소살균에 비해 약 1.2~1.4 배 정도 효율이 높게 나타났다. 또한 다제 내성플라스미드 pB10에 대한 제거 실험에서 오존에 의한 제거율이 염소보다 약 2~4배 높게 나타났다. 오존살균에 의한 높은 pB10 제거효율은 오존 살균시 발생하는 OH 라디칼에 의한 것으로 사료된다. 이러한 연구결과로부터 내성균 및 유전물질을 효과적으로 제어하기 위하여 기존 염소살균법에 오존 또는 광촉매산화와 같은 고급산화법을 연계처리에 대한 필요가 있을 것으로 판단된다.

• Journal of Microbiology and Biotechnology
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• 제26권1호
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• pp.130-138
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• 2016

The use of antibiotics in aquaculture raises environmental and food safety concerns because chronic exposure of an aquatic ecosystem to antibiotics can result in the spread of antibiotic resistance, bioaccumulation of antibiotics in the organisms, and transfer of antibiotics to humans. In an attempt to overcome these problems, high-concentration red clay was applied as an alternative antibiotic against the following common fish pathogens: Aeromonas salmonicida, Vibrio alginolyticus, and Streptococcus equinus. The growth of A. salmonicida and V. alginolyticus was retarded by red clay, whereas that of S. equinus was promoted. Phase contrast and scanning electron microscopy analyses confirmed the attachment of red clay on cell surfaces, resulting in rapid gravitational removal and cell surface damage in both A. salmonicida and V. alginolyticus, but not in S. equinus. Different cell wall properties of grampositive species may explain the unharmed cell surface of S. equinus. Significant levels of oxidative stress were generated in only the former two species, whereas significant changes in membrane permeability were found only in S. equinus, probably because of its physiological adaptation. The bacterial communities in water samples from Oncorhynchus mykiss aquacultures supplemented with red clay showed similar structure and diversity as those from oxytetracycline-treated water. Taken together, the antibiotic effects of high concentrations of red clay in aquaculture can be attributed to gravitational removal, cell surface damage, and oxidative stress production, and suggest that red clay may be used as an alternative for antibiotics in aquaculture.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제44권3호
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• pp.103-106
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• 2018

Objectives: The goal of the study was to investigate the clinical effects of amoxicillin-clavulanic acid (500+125 mg) with metronidazole 400 mg administered three times daily (Group I) versus azithromycin 500 mg administered once daily and with metronidazole 400 mg three times daily (Group II) for the prevention of postoperative infection following mandibular third molar surgical removal. Materials and Methods: The study design was a single-center prospective study. Patients who reported to the Department of Oral and Maxillofacial Surgery between February 2015 and January 2017 for removal of mandibular third molar were screened, and 108 patients were chosen. One surgeon carried out all procedures. Patients were prescribed antibiotics until the two groups contained a similar number of cases. Results: Our data showed that Group II had fewer incidences of surgical site infection, but with no statistical significance. Conclusion: Although both treatments are used routinely after removal of the mandibular third molar, neither is significantly better than the other.

• Membrane and Water Treatment
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• 제11권4호
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• pp.313-321
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• 2020

ACF preparation from different materials and its application in constructed wetlands for wastewater treatment has been focused in environmental field. Different materials have been used to prepare ACF around the world. This study aims to prepare, characterize and use of ACF from loofah for removal of Tetracycline from water through composite vertical flow constructed wetlands. ACF was prepared and it was tested for characterization, later it was used for removal of Tetracycline from water through composite vertical flow constructed wetlands. In composite vertical flow constructed wetlands, three HRTs were set according to the experiment, 1D, 2D, and 3D is individually. Samples were transported immediately from collection point to laboratory for analyzing. Samples were measured for Tetracycline (TC), Total Phosphorus (TP), and Total nitrogen and COD. Tetracycline absorbance with respective 356nm was obtained good and HRT is important factor. Results show that composite vertical flow constructed wetlands with ACF from luffa is best option and it is recommended to study further deep analysis.

• Advances in environmental research
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• 제4권1호
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• pp.49-68
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• 2015

Chemically activated and carbonized adsorbents were prepared from Moringa oleifera pod husks (MOP), characterized and evaluated for their ability to remove a common antibiotic - ofloxacin (OFX) from aqueous solution. The pulverized precursor was steeped in a saturated ammonium chloride solution for a day to give the chemically activated adsorbent (AMOP). A portion of AMOP was pyrolyzed in a muffle furnace at 623 K for 30 min to furnish its carbonized analogue (CMOP). The adsorbents showed favorable physicochemical attributes. The effects of operational parameters such as initial OFX solution pH and concentration, adsorbent dosage, temperature and contact time on OFX removal were investigated. At equilibrium, optimal removal efficiencies of 90.98% and 99.84% were achieved at solution pH 5 for AMOP and CMOP, respectively. The equilibrium adsorption data fitted into both the Langmuir and Freundlich isotherms. Gibbs free energy change (${\Delta}G^o$), enthalpy change (${\Delta}H^o$) and entropy change (${\Delta}S^o$) indicated that the adsorption of OFX was feasible, spontaneous, exothermic and occurred via the physisorption mode. Adsorption kinetics obeyed the Blanchard pseudo-second-order model. The results may find applications in the adsorptive removal of micro-contaminants of pharmaceutical origin from wastewater.

• 대한환경공학회지
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• 제31권2호
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• pp.96-101
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• 2009

생물활성탄 재질별 sulfonamide계 항생물질 5종의 생분해능을 조사한 결과 유입수의 수온 $25^{\circ}C$에서 EBCT 변화에 따른 생물분해율을 평가한 결과, 석탄계 재질의 생물활성탄은 EBCT 5~20분에서 18~82%, 야자계나 목탄계의 경우는 11~67% 및 4~56%로 나타나 석탄계 재질의 생물활성탄에서 가장 높은 생물분해능을 나타내었다. Sulfonamide계 5종에 대한 물질별 생분해능을 평가한 결과, 석탄계 재질의 생물활성탄에서 5~20분의 EBCT에서 SCP, SMT 및 STZ는 30~80%, SDM과 SMX는 18~70% 정도의 생분해율을 나타내어 SDM과 SMX가 다른 3종 보다는 생물분해능이 낮은 것으로 나타났다. 유입수의 수온 상승에 따라 5~20분의 EBCT에서 생물분해율은 크게 증가하였고, 유입수의 수온이 $5^{\circ}C$일 경우 sulfonamide계 항생물질 5종에 대한 생물분해 속도 상수는 0.0094~0.0118 $min^{-1}$, 반감기는 58.7~73.7분으로 나타났으며, 수온이 $15^{\circ}C$ 및 $25^{\circ}C$일 경우는 생물분해 속도상수가 0.0307~0.0397 $min^{-1}$ 및 0.0468~0.0718 $min^{-1}$로 나타났고, 반감기 17.5~22.6분 및 9.7~14.8분으로 나타났다. 유입수의 수온이 $5^{\circ}C$에서 $15^{\circ}C$로 상승할 경우 sulfonamide계 항생물질 5종에 대한 생물분해 속도상수는 3.2~3.8배 정도 증가하였으며, 수온이 $15^{\circ}C$에서 $25^{\circ}C$로 상승할 경우는 1.5~1.9배 정도 증가하는 것으로 나타났다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제35권6호
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• pp.396-401
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• 2013

Chronic osteomyelitis is an infection and inflammation of the bone or bone marrow, causing ischemia in bone marrow due to lack of blood, nutrients, and oxygen supply to the bone marrow, eventually leading to necrosis of bone marrow. A current method for treatment of chronic osteomyelitis is administration of systemic antibiotics followed by removal of the infected bone and tissues. Because infected tissue of chronic osteomyelitis is surrounded by avascular necrotic bone, supply of blood and antibiotics to the infected area is diminished. For effective treatment, high plasma concentrations of antibiotics should be provided for a prolonged period. However, long term high serum level of antibiotics may result in undesirable adverse effects. For delivery of a sufficient concentration of antibiotic to the infected area while avoiding the adverse effect, implantation of a local antibiotic delivery system is suggested. One of the implantation systems that has been utilized is antibiotic impregnated polymethyl methacrylate.

• 대한소아치과학회지
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• 제40권3호
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• pp.216-222
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• 2013

감염된 미성숙 영구치의 치수치료에 있어 줄기세포의 분화를 유도하는 생활조직의 재생과 지속적인 치근형성을 도모하는 방향으로 패러다임이 전환되고 있는데, 여기에서는 소독, 스캐폴드(scaffold), 그리고 폐쇄가 중요하다. 소독을 위해 triple antibiotics가 널리 사용되고 있으며, 스캐폴드로써 기존의 혈병대신 platelet-rich fibrin의 사용이 보고되었다. 본 증례보고에서는 치외치 파절에 의해 치수가 감염된 미성숙 영구치에서 platelet-rich fibrin을 스캐폴드로써 이용한 치수 재혈관화를 시행하였다. 발수와 근관세척 후 첫 증례에서 국소적 항생제의 적용 없이 platelet-rich fibrin을 단독 사용하였고 두 번째와 세 번째 증례에서는 국소적 항생제 적용 후 platelet-rich fibrin을 적용하였는데 모두 양호한 치유 결과를 얻었다.

• Journal of Yeungnam Medical Science
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• 제25권2호
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• pp.171-174
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• 2008

We report an unusual case of a sigmoid colon perforation after ventriculoperitoneal shunt surgery. Distal catheters are known to cause perforation in the setting of colonoscopy. The exact pathogenesis of this complication is not clear, but it can cause serious complications. Hence, patients require prompt and aggressive management, including laparotomy with bowel wall repair, catheter removal, and antibiotic therapy.