Postantibiotic Effects and Postantibiotic Sub-MIC Effects of Antibiotics on Fusobacterium nucleatum and Porphyromonas gingivalis

  • Hong, Jin (Department of Oral Microbiology, College of Dentistry, Research Institute of Oral Science, Kangnung National University) ;
  • Lee, Si-Young (Department of Oral Microbiology, College of Dentistry, Research Institute of Oral Science, Kangnung National University)
  • Published : 2005.06.30

Abstract

Postantibiotic effects (PAE) refer to suppression of the bacterial growth following limited periods of exposure to an antibiotic and subsequent to the removal of the antibiotic agent. Fusobacterium nucleatum and Porphyromonas gingivalis are Gram-negative anaerobic bacteria associated with several periodontal diseases. In this study, postantibiotic effects (PAE), postantibiotic sub-MIC effect (PA SME) and sub-MIC effect (SME) of antibiotics on F. nucleatum ATCC 25586 and P. gingivalis W50 were investigated. The PAE was induced by 10X the MIC of antibiotic and antibiotic was eliminated by washing. The PA SMEs were studied by addition of 0.1, 0.2 and 0.3X MICs during the postantibiotic phase of the bacteria, and the SMEs were studied by exposition of the bacteria to antibiotic at the sub-MICs only. Amoxicillin, doxycycline and tetracycline induced PAE for F. nucleatum ATCC 25586 and P. gingivalis W50. But metronidazole and penicillin induced PAE for only F. nucleatum ATCC 25586. Metronidazole and doxycycline induced PA SME and SME for both species of anaerobic bacteria used in this study. The PA SME values for both strains were substantially longer than the SME values. The present study showed the existence of PAE, PA SME and SME for various antibiotics against F. nucleatum ATCC 25586 and P. gingivalis W50.

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References

  1. Bigger, J.W.: The bactericidal action of penicillin on Staphylococcus pyogenes. Ir. J. Med. Sci. 227:533-568, 1944
  2. Cars, O. and Odenholt, T.l.: The postantibiotic sub-MIC effect in vitro and in vivo. J. Antimicrob. Chemother. 31:159-166, 1993
  3. Craig, W. A.: Pharmacokinetic/pharmacodynamic parameters: rationale for antibacterial dosing of mice and men. Clin. Infect. Dis. 26:1-12, 1998 https://doi.org/10.1086/516284
  4. Craig, W.A. and Gudmundsson, S: The postantibiotic effect. Antibiotics in laboratory medicine: 403-31, 1991
  5. Gottfredsson, M., Erlendsdottir, H. and Gudmundsson, S.: Different patterns of bacterial DNA synthesis during the postantibiotic effect. Antimicrob. Agents Chemother. 39: 1314-1319, 1995 https://doi.org/10.1128/AAC.39.6.1314
  6. Gudmundsson, S., Einarsson, S., Erlendsdottir, H., Moffat, T., Bayer, W. and Craig, W.A.: The post-antibiotic effect of antimicrobial combinations in a neutropenic murine thigh infection model. J. Antimicrob. Chemother. 31(SuppleD): 177-191, 1993 https://doi.org/10.1093/jac/31.1.177
  7. Karlowsky, J.A., Zhanel, G.G., Davidson, R.J. and Hoban, D.J.: Postantibiotic effect in Pseudomonas aeruginosa following single and multiple aminoglycoside exposures in vitro. J. Antimicrob. Chemother. 33:937-947, 1994 https://doi.org/10.1093/jac/33.5.937
  8. Lee, S.Y.: Postantibiotic effects and postantibiotic sub-MIC effects of amoxicillin on Strptococcus gordonii and Streptococcus sanguis. J. Chemother. 12:379-384, 2000 https://doi.org/10.1179/joc.2000.12.5.379
  9. Li, R.C., Lee, S.W. and Kong, C.H.: Correlation between bactericidal activity and postantibiotic effect for five antibiotics with different mechanisms of action. J. Antimicrob. Chemother. 40:39-45, 1997 https://doi.org/10.1093/jac/40.1.39
  10. Lowdin, E., Odenholt, T.I., Bengtsson, S. and Cars, O.: A new method to determine postantibiotic effect and effects of subinhibitory antibiotic concentrations. Antimicrob. Agents Chemoter. 37:2200-2205, 1993 https://doi.org/10.1128/AAC.37.10.2200
  11. Odenholt, T.l.: Pharmacodynamic effects of sub inhibitory antibiotic concentrations. Int. J. Antimicrob. Agent. 17: 1-8, 2001 https://doi.org/10.1016/S0924-8579(00)00243-0
  12. Odenholt, T.I., Lowdin, E. and Cars, O.: Postantibiotic sub-MIC effects of vancomycin roxithromycin, sparfloxacin, and amikacin. Antimicrob. Agents Chemother. 36: 1852-1858, 1992 https://doi.org/10.1128/AAC.36.9.1852
  13. Socransky, S.S., Haffajee, A.D., Cugini, M.A., Smith, C. and Jr, K.R.: Microbial complexes in subgingibal plaque. J. Clin. Periodontol. 25: 134-144, 1998 https://doi.org/10.1111/j.1600-051X.1998.tb02419.x
  14. Zhanel, G.G., Hoban, D.J. and Harding, G.K.: The postantibiotic effect. DICP. 25:153-63, 1991 https://doi.org/10.1177/106002809102500210