• Microbiology and Biotechnology Letters
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• v.46 no.1
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• pp.40-44
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• 2018

An isolate capable of inhibiting the growth of gram-positive bacteria was obtained from the soil of Mushim stream, Cheongju. The isolate was identified as Pseudomonas otitidis PS by 16S rRNA gene sequence analysis. P. otitidis PS produced antibiotics as a secondary metabolite when cultured in 1% soybean meal with 0.5% glucose. The maximum yield was about 0.1%. The antibiotic substance of P. otitidis PS extracted using ethyl acetate displayed a minimum inhibitory concentration of $2{\mu}g/ml$ for Staphylococcus aureus KCTC 1261. The antibiotic substance produced an orange halo on chrome azurol S agar due to siderophore activity. Growth inhibition was decreased when the iron was depleted. Since the antibiotic activity was lost upon the addition of the reducing agent ascorbic acid or during anaerobic culture, it was considered that antibiotic of P. otitidis PS strain exerts its bactericidal effect by the generation of reactive oxygen species.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.30 no.2
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• pp.100-111
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• 2017

Objectives : The purpose of this study was to research the antibiotic, antioxidant and whitening effects of Jwa Kum-Whan(JKW) and Soo Ryeon-Whan(SRW). Both Jwa Kum-Whan and Soo Ryeon-Whan are composed of Coptidis rhizoma and Evodiae fructus, but the ratios of the two species are different. JKW is composed of Coptidis rhizoma and Evodiae fructus by ratio of 6:1 and SRW's ratio is 1:1. Methods : Antibiotic activities of JKW and SRW's water extracts were studied by paper disc diffusion method. Four kinds of bacteria were applied in paper disc, and each extract was dropped, individually. The diameter of inhibition zone was measured. DPPH assay was used for studying free radical scavenging activity. DPPH is quantitatively decolorized from purple color by antioxidant material. Change of color was measured by spectrophotometer. Whitening activity was analyzed by tyrosinase inhibition assay. Tyrosinase is major enzyme for control process of making eumelanin. Optical Density was measured by spectrophotometer. Results : Candida albicans and Staphylococcus aureus's inhibition zone were made large available by Both prescriptions. Inhibition zone's diameter of JKW was preferable to SRW's. Radical scavenging activity was better at SRW, but JKW's activity was available, too. Tyrosinase inhibition activity was found out available only for JKW, not for SRW. Conclusions : JKW had antibiotic effects for Candida albicans, Staphylococcus aureus. And had antioxidant, whitening effects. SRW had antibiotic, antioxidant activities but not whitening effect.

• Microbiology and Biotechnology Letters
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• v.20 no.2
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• pp.213-218
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• 1992

Fermentation patterns were changed by adding magnesium sulfate to the fermentation broth and its effect on enhancement of sisomicin production was investigated. When cell growth was expressed by DNA content, trophophase and idiophase were separated, but not by dry cell weight. On the other hand, addition of magnesium sulfate had the antibiotic accumulated inside the cells be liberated into the outside, and this effect resulted in improving the final antibiotic yield. The maximum antibrotic yield was obtained when 100 mM magnesium sulfate was added after one day of cultivation, and enhanced more than three times compared to that of the control to which it was not added.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.11
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• pp.1656-1660
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• 2003

The aim of this study was to investigate the effect of fermented fruits (FF) on the growth performance, Enterobacteriaceae and Lactobacillus counts in faeces of the post-weaning piglets. A total of twenty-four 4 weeks old Landrace${\times}$ Large White${\times}$Duroc with initial body weight of 6 kg were used in this study. The piglets were housed individually in metabolic cage and randomly assigned to four groups with six piglets per group. The piglets were fed on basal diet without antibiotic (AF), basal diets with antibiotic (Ab), basal diet with 10% (w/w) fermented fruit (10% FF) and basal diet with 20% (w/w) fermented fruit (20% FF). Faecal samples were taken directly from the rectum of each piglet and cultured for Enterobacteriaceae and Lactobacillus counts. In the growth performance, the piglets of Ab and 10%FF had significantly higher (p<0.05) average daily gain than those of 20%FF. However, no differences (p>0.05) were observed between AF, Ab and 10%FF. Studies showed that the use of fermented fruits (FF) could significantly (p<0.05) reduce Enterobacteriaceae population in piglets' faeces compared to the use of normal feed (AF) and antibiotic (Ab). However, the Lactobacillus population in the faeces was increased in those piglets fed with diets added with FF.

• Korean Journal of Plant Resources
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• v.30 no.3
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• pp.311-317
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• 2017

This study was conducted with the aim of evaluating the antibiotic effects of leaves, stems, and roots in yacon (Smallanthus sonchifolius). Antibacterial activity of leaf extract by disk diffusion method with Bacillus subtilis and Escherichia coli respectively showed 13.3 and 13.75mm diameters of clear zone. There was no significant difference between the stems and leaves. The minimum inhibitory concentration of leaves' heating and agitation extraction showed a restrain of strain at 1mg/ml, but the stems and root extract did not appear. Yacon is a functional antibacterial material, and methanol extraction is more effective than water. This research was to investigate the growth stage of collection has the most effective antibacterial effects. It has collected yacon's leaves from June to October, which is an appropriate time for collection right before reaping. Yacon leaf has antibacterial effects on Bacillus subtilis, Escherichia coli, Enterococcus faecium, and Salmonella enteritifis. There were no significant differences by the growth stage of collection. Leaves collected in July are high in phenol which helps in sulfating activity works well considering the high scavenging capability of DPPH. Leaves collected in September are high in total flavonoid.

• Microbiology and Biotechnology Letters
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• v.47 no.2
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• pp.173-182
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• 2019

The use of double lumen catheters as a means of hemodialysis access is commonly accompanied with the use of gentamicin as an antibiotic lock. Other antibiotics and anticoagulants are often added to increase the efficacy of gentamicin in order to reduce catheter-related infection and to prevent biofilm formation. This review aimed to evaluate the following: 1) the use of gentamicin in eliminating catheter-related infection and reducing biofilm formation in hemodialysis catheters, 2) the efficacy of additional antibiotics in combination with gentamicin, and 3) the effect of additional anticoagulants to complement the efficacy of gentamicin as the main prophylactic antibiotic lock. We sorted through data from 242 PubMed and ScienceDirect studies, which were then short-listed to 33 studies. Next, they were grouped, extracted, and analyzed qualitatively to fulfil the objectives of this review. Consequently, the use of a gentamicin-lock solution was shown to reduce the incidence of bacteremia; however, it was not strong enough to inhibit the growth of infectious microbes and formation of biofilms. Several bacteria, such as Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, and Klebsiella pneumoniae, have been reported as infectious agents. Combination with other antibiotics also provided no effect in reducing bacterial growth and biofilm formation in catheters. Furthermore, the additional anticoagulants (trisodium citrate and EDTA) were reported to be effective in enhancing the efficacy of gentamicin in avoiding catheter-related infection, bacterial growth, and biofilm formation; thus, the use of gentamicin can be rationalized.

• Korean Journal of Microbiology
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• v.15 no.4
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• pp.154-158
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• 1977

Fifteen kinds of antibiotics both in liquid or dry stantes wre investigated for the purpose of radiation effect on the antibiotic activities after irradiation. In liquid states, the antibiotics such as amoxyllin, ampicillin, capharolidin and hetacillin were inctivated 90% of their antibiotic activities at radiation doses of 50Krad, and penicillin, dicloxacillin, caphalothin, cloxacillin, erythromycin and caphalexin and kanamycin were decomposed at radiation doses of 1.5Mrad, respectively. In dry states, all antibiotics were stable at radiation doses of 5Mrad. On the other hand, the neomycin in liquid state was rather increased the antiboctic activity about 1.2 and 8 times when the E. coli ATCC 113-3 and B. subtitilis ATCC 6633 were used aas a standard microorganisms, whereas, the decrensed antibiotic activities were observed in S. typhi Ty-2(36.2%), St.faecium(10.79%), B.sphaericus CIA(9.066%) and St.aureus ATCC 6538p(70%).

• Fibers and Polymers
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• v.7 no.1
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• pp.1-7
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• 2006

Interaction of a representative antibiotic, doxycycline(Doxy), with commercial poly(acrylonitrile) (PAN) and cationic-dyeable poly(ethylene terephthalate)(PET) fiber was studied in development of infection resistant biomedical materials. Regular PET was also employed for a comparison purpose. Their interactions were investigated at different treatment temperatures, times, and pHs. Fibers were also hydrolyzed by 1% NaOH for 1 or 2 hours at $85^{\circ}C\;and\;100{\circ}C$ to study effect of hydrolysis on antibiotic sorption. Infection-resistant characteristics of the substrates were evaluated by zone of inhibition (ZOI) test. Results revealed that a significant chemical change occurred in PAN and cationic-dyeable PET due to hydrolysis. Additional functional groups obtained by hydrolysis not only enhanced sorption of the antibiotics but also provided greater ZOI values, indicating substantial improvement in sustained infection resistance properties.

• Advances in environmental research
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• v.7 no.3
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• pp.201-212
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• 2018

In this study, conditions of solid phase extraction (SPE) for determination of some antibiotics such as trimethoprim, oxytetracycline, erythromycin, clarithromycin, azythromycin, doxycycline, sulfamethazine, ciprofloxacin, chlortetracycline, sulfamethoxazole in wastewaters were optimized. After the optimum volume and pH of the sample were determined, the effect of the concentration of the compounds and matrix were investigated. The highest recovery rates for antibiotic compounds were determined between 82% and 105% in 200 mL sample volume and pH 2.5. Then, antibiotic compounds were investigated in influent and effluent samples taken from Konya Urban Wastewater Treatment Plant. The concentration of the antibiotics was detected range of 0.11-101 ng/L in influent waters and

• Journal of the Korea Fashion and Costume Design Association
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• v.23 no.4
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• pp.95-103
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• 2021

This study aims to examine the antibacterial effects of cotton and silk fabrics naturally dyed with Artemisia princeps extract on antibiotic-resistant strains of bacteria. The concentrated natural dye of the Artemisia princeps extract was made at the liquor ratio of 1:10 at 40-60℃ for 60 minutes. The concentration of FeSO4·7H2O, Al2(SO4)3, and CuSO4 5H2O mordant was 3% (owf), and the liquor ratio was 1:20. In order to experiment on the antimicrobial activity of the naturally dyed fabrics, Methicillin-resistant Staphylococcus aureus (MRSA) ATCC 33591, was used by breeding it in Brain Heart Infusion Agar (BHA) containing Oxacillin (2㎍/ml), Fungizone (2.5㎍/ml), and Brain Heart Infusion broth (BHI; Detroit, MI, USA). As a result of examining the bacterial growth reduction rate on dyed cotton and silk fabrics against antibiotic-resistant strains, it was found that the copper mordant in cotton fabric shows the highest antibacterial activity with a bacterial growth reduction rate of 99.9%, and the non-mordant cotton fabric shows the lowest antibacterial activity with a reduction rate of 18.6%. In the case of the naturally dyed silk fabric, it indicates the highest reduction rate of strains in the Al mordanting (94.9%), and Cu mordanting (99.9%).