• 제목/요약/키워드: antibiosis

검색결과 130건 처리시간 0.024초

Phytophthora capsici의 성장을 저해하는 Enterobacter sp. B54의 선발과 Tn5 lac을 이용한 돌연변이 유기 (Tn5 lac Mediated Mutagenesis of Enterobacter sp. B54 Antagonistic to Phytophthora capsici.)

  • 윤상홍;최청
    • 한국미생물·생명공학회지
    • /
    • 제26권5호
    • /
    • pp.393-399
    • /
    • 1998
  • 고추역병균(Phytophthora capsici)의 성장을 in vitro에서 저해하는 길항균 Bl4를 한국토양으로부터 분리 동정하여 Enterobacter속임을 밝혔고 Pl::Tn5 lac에 의해 transposon돌연변이를 유기하여 길항력 강화주와 약화주들의 염색체내에 Tn5 lac이 각기 상이한 위치에 무작위로 삽입되었음을 southern hybridization에 의해 확인하였다.

  • PDF

오디분말의 추출 방법에 따른 생리활성 및 항균효과에 미치는 영향 (Effects of Extraction Method on Physiological Activity and Antibiosis of Mulberry Powder)

  • 김송기;강근옥
    • 동아시아식생활학회지
    • /
    • 제22권3호
    • /
    • pp.392-400
    • /
    • 2012
  • The purpose of this study was to analyze the physiological activity and antibiosis of mulberry powder by extract method. The total phenol content of mulberry powder extracted with methanol was 82.9 mg/g, while that of powder extracted with water was 46.9 mg/g. Extractions with methanol were therefore more effective than with water. The total flavonoid contents of mulberry powder extracted with methanol was 13.0 mg/g, while that of powder extracted with water was 9.4 mg/g. Also, the nitrite-scavenging ability of mulberry powder was lower than ascorbic acid and BHT. The SOD-like activity of mulberry powder extracts, natural antioxidant, and artificial antioxidant at 5 mg/mL arranged in order of decreasing concentration were ascorbic acid (98.3%) > BHT (88.1%) > water extract (9.8%) > methanol extract (3.0%). And, the OH radical scavenging activities of mulberry powder extracts and natural antioxidant at 5 mg/mL in order of decreasing concentration was ascorbic acid (97.0%) > methanol extract (46.2%) > water extract (35.8%). The antimicrobial effects of mulberry powder extracted with methanol could be detected on Bacillus cereus ($10,000{\mu}g/disc$) and Staphylococcus aureus ($10,000{\mu}g/disc$).

아유르베다 오일의 항균성에 관한 연구 (A Study of the Antibiosis in Ayurvedic Oils)

  • 최정명
    • 한국산학기술학회논문지
    • /
    • 제11권3호
    • /
    • pp.1139-1145
    • /
    • 2010
  • 본 연구는 아유르베다의 모발과 두피 프로그램을 적용하고 그 효과를 분석하고자 한다. 아유르베딕(Ayurvedic) 처치는 자연치유력에 의해 면역력을 강화시킨다. 아유르베딕 베이스 오일과 에센셜 오일을 모발과 두피에 사용했을 때, 두피에 상주하는 균에 대한 항균효과를 알아보기 위해 MIC test 와 Halo test를 통해 측정하였다. 그 결과는 다음과 같다. 9종류의 베이스 오일과 18종류의 에센셜 오일을 이용하여 Bacillus subtilis, Propionibacterium acnes균에 대한 베이스 오일과 에센셜 오일의 항균효과를 MIC test를 통해 조사하였다. 실험 결과, 베이스 오일에서는 모두 균에 대한 항균효과가 없는 것으로 나타났으며, 에센셜 오일은 Chamomile, Clary sage, Jasmine, Neroli, Rose오일에서 두 균에 대해 모두 5% 이하에서 항균효과가 없는 것으로 나타났다. 두 균주에 대해 Thyme, Geranium, Lavender, Tea tree가 모두 효과가 높은 것으로 조사되었다. Geranium, Lavender, Thyme 에센셜 오일과 Sesame and Coconut 베이스 오일을 이용하여 7가지 공시균주에 대한 Halo test결과 베이스 오일에서는 항균효과가 없는 것으로 나타났다. 에센셜 오일은 7가지 공시균주에 대해 모두 항균성이 있는 것으로 조사되었다. Thyme은 MIC test 와 Halo test 결과 항균력이 가장 높은 것으로 나타났다.

초임계 이산화탄소를 이용한 마늘 추출물의 항균효과에 관한 연구 (A Study on the Antimicrobial Effect of Garlic Extract using Super-Critical Carbon Dioxide)

  • 성기천
    • 한국응용과학기술학회지
    • /
    • 제20권1호
    • /
    • pp.51-56
    • /
    • 2003
  • Garlic extract using super-critical carbon dioxide is influenced by temperature and pressure, and the optimum condition can make under super-critical state. We can know the defects in process of super-critical extration, It can indicate the drop of product rate, energy loss and equipment expense etcs. The minimum inhibition concentration of microbe which garlic extract contains has apperared the concentration more than 800ppm in this experiment. According to the result of this experiment, we can know that the antibiosis effect in the microbe of staphylococcus and fungus has disappeared in the incubation time more than 12 hours.

Identification and Characterization of Bdellovibrio bacteriovorus, a Predator of Burkholderia glumae

  • Song, Wan-Yeob
    • Journal of Microbiology and Biotechnology
    • /
    • 제14권1호
    • /
    • pp.48-55
    • /
    • 2004
  • Six strains of an obligate predatory bdellovibrio isolate that preys on Burkholderia glumae in rice paddy field water and rhizosphere soil, were identified and characterized. The numbers of Bdellovibrio cells varied from $3.2{\times}10^3$ to $9.2{\times}10^3$ plaque-forming unit/g after enrichment in cells of B. glumae. Prey range tests with six Bdellovibrio strains and 17 prey strains of rice-pathogenic, antibiosis-related, or nitrogen-fixing bacteria resulted in unique predation patterns in related prey cells. Strain BG282 had the widest prey range on 7 plant pathogenic bacteria among the 17 prey strains tested. However, no predation occurred with strains of Azospirillum brasilense, Paenibacillus polymyxa, Pseudomonas fluorescens, P. putida, and Serratia marcescens that are associated with antibiosis or nitrogen fixation in the rice ecosystem. Identification was confirmed by the presence of typical bdelloplast in the prey cells of B. glumae and by a PCR assay using B. bacteriovorus-specific primers. Furthermore, 16S rDNA sequencing of the six bdellovibrio strains showed a homology range of 97.2% to 99.2% to the type strain of B. bacteriovorus.

어성초(Houttuynia cordata Thunb.)의 향약성 효과와 이용에 관한 고찰 (Literature Review on the Pharmaceutical Effects and Utilization of Houttuynia cordata Thunb)

  • 안빈
    • 동아시아식생활학회지
    • /
    • 제12권1호
    • /
    • pp.1-6
    • /
    • 2002
  • Houttuynia cordata Thunb. grown in wet earth in China, Japan and Korea, has often been cited in medical literature for its medicinal effects. In this paper, the pharmaceutical effects of Houttuynia cordata Thunb., featured in the East Asian literature were studied. It was revealed that it has a wide variety of uses, including remedial treat-ments for pneumonia, antidote, inflammation, syphilis, abscess, paralysis and gynecological diseases. Its application methods also are numerous: drinking as tea or squeezed juice, application to wounds, wet dressing, chewing raw roots and mixing with other plants, liquor and food materials. The major pharmaceutical effects are as follows: antibiosis, immunity enhancement, urination, pain relieving, vasodilator and cough lozenge.

  • PDF

한국산 메주에서 분리된 Aspergilli의 항생물질 생산에 관한 연구 (Studies on the productivity of antibiotic produced by Aspergilli which were lsolated from "Meju".)

  • 김지경;이배함
    • 미생물학회지
    • /
    • 제5권2호
    • /
    • pp.86-92
    • /
    • 1967
  • By cylinder-plate method was the antibiosis of 15 strains of Aspergilli examined, which were isolated from Korean "Meju" collected through all over South-Korea. The results are as follows; 1) Only two strains of Aspergilli (strain 1 and 9) sellected in screening test have antibiosis for the several gram positive and negative bacteria. 2) The activity of antibiotic produced by strain I was excellent, having been cultured at 25.deg.C in the Malt-infusion Czapek's medium of pH 3.5 to 7.5 for 6 days. 3) The activity of antibiotic produced by strain 9 was excellent, having been cultured at 25.deg.C in the Czapek's medium of pH 4.5 to 6.5 for 6 days. 4) When Aspergilli were cultured under optimum conditions which resulted, by experiments, the activity of antibiotic produced by Aspergilli was stronger than activity of 10.gamma./ml. streptomycin.eptomycin.

  • PDF

견직물의 자초 염색 시 합성탄닌의 매염 효과 (Effect of Tannin Mordanting on Gromwell-dyed Silk Fabric)

  • 박아영;송화순;김인영
    • 한국염색가공학회지
    • /
    • 제20권6호
    • /
    • pp.51-62
    • /
    • 2008
  • The purpose of this study was to check color change depending on dyeing conditions when silk fabrics was dyed with gromwell and to investigate mordant effect when synthetic tannin was used as a mordant. First, when silk fabrics were dyed with gromwell, the change of color, brightness and chroma were examined by measuring K/S value, a, b value, L value and C value depending on dyeing condition. Second, color, brightness and chroma differences which appeared after mordanting with synthetic tannin were investigated and muti-functional mordanting effect was confirmed with dyeing fastness, antibiosis. As a result of color analysis of gromwell, it was proved that the main pigment of gromwell was shikonin. Color was red purple at pH 3 when silk fabrics were dyed with gromwell. But It became greenish and bluish as temperature rose and time passed by. And it grew reddish and yellowish as concentration level reached higher. The color became purple at pH 5 and purple blue at pH 7. Both at pH 5 and pH 7, it became greenish and yellowish, as temperature went up and time ran by. And it became reddish with concentration increasing. Brightness and chroma decreased with temperature and concentration increasing as well as time passing by. After mordanting with synthetic tannin, the color became less reddish at pH 3 and reddish-yellowish at pH 5 and pH 7. The brightness increased and the chroma decreased. At all pH, the color, the brightness and the chroma became similarly by synthetic tannin mordanting. Dyeing fastness of synthetic tannin mordanted fabric was higher than that of non-mordanted fabric. Especially, wet fastness at pH 3 improved from 1 grade before synthetic tannin mordanting to $3{\sim}4$ grade after tannin mordanting. Antibiosis was improved by gromwell dyeing and synthetic tannin mordanting.

Positional mapping for foxglove aphid resistance with 180k SNP array in soybean [Glycine max (L.) Merr.]

  • Park, Sumin;Kim, Kyung Hye;Go, Hong Min;Lee, Ju Seok;Jung, Jin Kyo;Bilyeu, Kristin D.;Lee, Jeong-Dong;Kan, Sungtaeg
    • 한국작물학회:학술대회논문집
    • /
    • 한국작물학회 2017년도 9th Asian Crop Science Association conference
    • /
    • pp.145-145
    • /
    • 2017
  • Foxglove aphid, Aulacorthum solani (Kaltenbach), is a Hemipteran insect that infected a wide variety of plants worldwide and caused serious yield losses in crops. The objective of this study was to identify the putative genes to foxglove aphid resistance in wild soybean, PI 366121 (Glycine soja Sieb. and Zucc.). One hundred and forty-one F4:8 recombinant inbred lines developed from a cross between susceptible variety, Williams 82 and foxglove aphid resistance wild soybean, PI 366121 were used. The two type of resistance response, antibiosis and antixenosis resistance were evaluated through choice and no-choice test, graded by the degree of total plant damage and primary infestation leaf damage; a genome-wide molecular linkage map was constructed with 29,898 single-nucleotide polymorphism markers utilizing a Axiom(R) 180K soyaSNP array. Using inclusive composite interval mapping analysis for foxglove aphid resistance, one major candidate QTL on chromosome 7 was identified. The major QTL on chromosome 7 showed both antixenosis and antibiosis resistance responses. The newly identified major QTL was consistent with previously reported QTL, Raso2, which showed around 5 times narrow down interval range with 8 candidate genes. Furthermore, total 1,115 soybean varieties including Glycine soja and Glycine max were exposed to germplasm screening, and 31 varieties, which showed significant antibiosis type foxglove aphid resistance were identified. This result could be useful in breeding for new foxglove aphid resistant soybean cultivars and developing novel insecticides.

  • PDF

끝동매미충에 대한 벼의 저항성 및 그 기작에 관한 연구 (Varietal Resistance of Rice to the Green Rice Leafhopper, Nephotettix cincticeps Uhler)

  • 최승윤
    • 한국응용곤충학회지
    • /
    • 제14권1호
    • /
    • pp.13-21
    • /
    • 1975
  • 1974년도 IRRI(국제미작연구소)에서 새로 입수된 48개 종품 및 게통을 공시하여 한국산 끝동매미충에 대한 저항성품종 및 게통을 선발하고 나아가 몇가지 저항성기작을 검토하기 위해 본 시험을 실하여 다음과 같은 결과를 얻었다. 1. 끝동매미충에 대한 유묘검정에서 48개 품종 또는 계통중 저항성반응(R)을 보인 것은 Balamawee, IR-26, IR-1529-525-2-2, IR-1539-1042-6, IR2061-214-2-3-5, IR2061-214-2-3-6, Jhingasail, Murungakayan 및 Vunam등 9개 품종 또는 계통이었고 중도저항성반응(MR)을 보인 것들로서는 Chianung-Shen-Yu-11, C62-1-230, C62-1-373, Dikwee, D204-1, Murungakayan-3, Palasithari-601, Peta-2802, RP6-516-34-1-8(TKM $6\times IR8$), RP6-590-10-1-11(TKM6$\times$IR8), RP9-4(IR8$\times$W1251)등 11개 품종 또는 계통이었다. 2. 저항성 및 중도저항성반응을 보인 것들을 공시하여 약충접종에 의한 저충성검정시험결과 저항성반응을 보인 것은 모두 $100\%$의 사충율을 보였으나 몇몇 품종 또는 계통에서 사충율이 좀 저하하였고 저조한 우화율을 나타내었다. 저항성반응을 보인 벼에서 2영기 약충이 살아 남을 수 있는 일수는 3-10일에 불과하였다. 즉, 유묘의 반응에서 저항성으로 나타난 벼들은 고도의 저충성이 있음을 보였다. 3. 저항성반응을 품종 또는 계통간에 차이가 없었을뿐더러 유묘의 저항성반응과도 아무런 상관이 없었다. 5. 이상의 결과를 종합해 볼 때 끝동매미충에 대한 벼의 저항성기작은 저항성 뼈는 고도의 항충성(Antibiosis)을 지니고 낮은 식이선호성(Non-feeding preference)을 나타내지만 감수성 벼는 반대로 항충성을 지니고 있지 않은데다가 높은 식이선호성을 나타내는데 있다고 보아진다.

  • PDF