• 펄프종이기술
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• 제44권3호
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• pp.34-40
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• 2012

In this study, new antibacterial materials were developed to manufacture a functional corrugated board. Sulfur solution, a new antibacterial solution made from inorganic sulfur in the laboratory, and other antibacterial mat erials were adopted to treat the surface of a linerboard. We measured the antibacteriocidal and bacteriostatic activities, as well as the fungal resistance of the surface-treated linerboards, to identify the antibacterial properties. The mechanical properties of the surface-treated linerboard were also determined in order to identify the effects of the antibacterial materials on linerboard properties. Linerboard treated with sulfur solution, PVOH, and sodium metasulfite showed the highest antibacterial activity, while linerboard treated with sulfur solution and nano sulfur showed the highest fungal resistance. It was identified that sulfur solution has effective antibacterial properties. The antibacterial materials did not affect the mechanical properties of the surface-treated linerboard, but the binder showed significant effects in terms of the burst strength, the compressive strength, and the stiffness of the linerboard.

• 생약학회지
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• 제53권2호
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• pp.87-95
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• 2022

Methicillin resistance Staphylococcus aureus (MRSA) is a gram-positive bacterium, the most commonly isolated bacterial human pathogen. JiYu-san is one of the natural products used to treat diseases in the folk recipe. In this study, we investigated the antimicrobial activity of EtOH 70% extracts of JiYu-san (JYS) against MRSA. The antibacterial activity of JYS against MRSA strain was evaluated using minimum inhibitory concentration (MIC), checkerboard dilution test, and time-kill assay. The effect of JYS on the immune mechanism of MRSA was confirmed through cell membrane permeability tests and energy metabolism tests, and the antibacterial activity mechanism was performed using qRT-PCR and western blot. As a result, in the antibacterial test of JYS, the MIC was measured to be 1.9~1000 ㎍/mL, and synergistic or showed a partial synergistic effect. In addition, JYS showed antibacterial activity in a combination test with DCCD or TX-100. In a study on the mechanism of action of antibacterial activity, it was found that JYS suppressed MRSA resistance genes and proteins. These results suggest that JYS has antibacterial activity and provides great potential as a natural antibiotic by modulating the immune mechanism against MRSA.

• Journal of Microbiology and Biotechnology
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• 제15권5호
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• pp.1039-1046
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• 2005

The salt resistance of antibacterial activity and synergistic effect with clinically used antibiotic agents are critical factors in developing effective peptide antibiotic drugs. For this reason, we investigated the resistance of antibacterial activity to antagonism induced by NaCl and $MgCl_2$ and the synergistic effect of P20 with cefotaxime. P20 is a 20-residue synthetic peptide derived from a cecropin A (CA)-melittin(ME) hybrid peptide. In this study, P20 was found to have potent antibacterial activity against clinically isolated methicillin-resistant Staphylococcus aureus (MRSA) strains without hemolytic activity against human erythrocytes. The combination study revealed that P20 in combination with cefotaxime showed synergistic antibacterial activity in an energy-dependent manner. We also confirmed the synergism between P20 and cefotaxime by fluorescence-activated flow cytometric analysis by staining bacterial cells with propidium iodide (PI) and bis-(1,3-dibutylbarbituric acid) trimethine oxonol (BOX). This study suggests that P20 may be useful as a therapeutic antibiotic peptide with synergistic effect in combination with conventional antibiotic agents.

• Journal of Microbiology and Biotechnology
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• 제18권5호
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• pp.990-996
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• 2008

In a previous study, we found an antifungal effect on human pathogenic fungi by the cell-penetrating peptide Tat (47-58) derived from HIV-1. Tat (47-58) immediately entered into the fungal nucleus and affected some physiological changes on the intracellular condition. In this study, Tat (47-58) showed a broad spectrum of antibacterial activity against pathogenic bacteria including bacterial clinical isolates. To improve resistance against proteases for use in vivo, we synthesized an analog of Tat (47-58) by substituting the L-amino acid for the D-amino acid. The D-enantiomer of Tat (47-58) also exhibited a broad spectrum of antibacterial activity at almost the same level of L-Tat (47-58) concentration. Unlike L-Tat (47-58), D-Tat (47-58) showed a significant proteolytic resistance against all proteases tested and antimicrobial activities in the presence of trypsin. Moreover, D-Tat (47-58) inhibited MRSA infection in human HeLa cells whereas L-Tat (47-58) partially allowed MRSA infection, and the results were due to the proteolytic resistance of D-Tat (47-58).

• 멤브레인
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• 제33권6호
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• pp.398-408
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• 2023

본 연구는 지카바이러스, 메르스, coronavirus disease-19 (COVID-19) 등의 감염병 방역 및 의료현장에서 사용할 수 있는 의료용 공기정화호흡기(powered air purifying respirators, PAPR)의 항균성 보호복의 후드와 필터를 개발하였다. PAPR은 전동팬 본체 및 필터, 배터리팩, 후드로 구성되며 보호복의 후드 소재는 뛰어난 흡습성, 풍압, 외부충격을 견딜 수 있는 폴리프로필렌 슐폰레이스(spunlace) 부직포 직물(SFS)을 사용하였다. 사용자의 감염위험을 낮추기 위해 후드의 외피에는 피톤치드계 물질을 사용하여 99.9%의 안티-박테리얼(antibacterial) 효과를 얻었으며 내피에는 친수가공을 하여 흡수성을 25% 향상시켰다. 의료용 보호복 후드에 필요한 인공혈액 침투저항성, 건조미생물 침투저항성, 습식세균 침투저항성, 그리고 박테리오파아지 침투저항성을 평가한 결과 2~6 단계의 합격평가를 받았다. 한편, 항균 처리된 슐폰레이스(spunlace filter, SF) 헤파 필터(high efficiency particulate air, HEPA)의 성능을 평가한 결과 우수한 항균성, 분진제거율, 차압 효과를 확인하였다.

• 대한한방내과학회지
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• 제21권4호
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• pp.555-563
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• 2000

Objective : In order to know the antibacterial effects of Kyungok-go against Mycobacteria tuberculosis, Methods : In this study, I inverstigated these effects in terms of combination of other antibiotics with and without Kyungok-go on several different media conditions. Results: On Ogawa medium, Kyungok-go of the $10{\mu}/ml$ concentration showed the anti-Mycobacteria tuberculosis activity against antibiotic drug-sensitive strain. On Middle-blue medium, Kyungok-go of the $10{\mu}/ml$ concentration showed the anti-Mycobacteria tuberculosis activity against antibiotic drug-sensitive strain. Kyungok-go showed the anti mycobacteria tuberculosis activity with the meaningful result above a certain concentration. The resistance against M, tuberculosis as the concentration of Kyungok-go was decreased significantlly on the high concentration($500{\mu}/ml$) When rifampicin and Kyungok-go were used together, the resistance was decreased with the statistical significance as to the persistant antibacterial effect against M. tuberculosis, When ciprofloxacin and Kyungok-go were used together, the resistance was decreased with the statistical significance as to the persistant antibacterial effect against M. tuberculosis, The combination of treatment, Kyungok-go with both rifampicin and ciprofloxacin, showed much better antibacterial effect against M, tuberculosis than antibiotics alone. Conclusions : This study shows that Kyungok-go has antibacterial effect against M. tuberculosis and in the combination of treatment, Kyungok-go with antibiotics, showed much better antibacterial effect against M. tuberculosis than antibiotics alone,.

• 펄프종이기술
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• 제33권3호
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• pp.59-68
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• 2001

Essential oils extracted from Chamaecyparis obtusa were applied to make antibacterial corrugating liner. The quantitative analysis of the essential oils was carried out by GC-MS, which showed that the monoterpenes as a basic component of the essential oils extracted from Chamaecyparis obtusa in the domestic mountain were greater in amount than those in China. The antibacterial activity was then examined with Tyromyces palustris and gram-negative/positive bacteria. The papers treated with the essential oils desplayed great resistance against T. Palustris and gram bacteria but the best results were obtained with the handsheet formed by softwood bleached kraft pulp rather than the liner by OCC. The low antibacterial activity of the liner was considered to be due to starch components which could be readily attacked by the fungi.

• Natural Product Sciences
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• 제24권4호
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• pp.225-228
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• 2018

By activity-guided fractionation, gliotoxin was isolated as an antibacterial metabolite of the fungus Penicillium decumbens which was derived from the jellyfish Nemopilema nomurai. Gliotoxin was further evaluated for antibacterial activity against several piscine and human MDR (multidrug resistance) pathogens. Gliotoxin showed significant antibacterial activity against Gram-positive piscine pathogens such as Streptococcus iniae FP5228, Streptococcus iniae FP3187, Streptococcus parauberis FP3287, Streptococcus parauberis SPOF3K, S. parauberis KSP28, and Lactococcus garvieae FP5245. Gliotoxin showed strong activity especially against S. parauberis SPOF3K and S. iniae FP5228, which are resistant to oxytetracycline. It is noteworthy that gliotoxin effectively suppressed streptococci which are the major pathogens for piscine infection and mortality in aquaculture industry. Gliotoxin also showed strong antibacterial activity against multidrug- resistant human pathogens (MDR) including Enterococcus faecium 5270 and MRSA (methicillin-resistant Staphylococcus aureus) 3089.

• ALGAE
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• 제36권1호
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• pp.61-72
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• 2021

Increased proliferation of bacterial resistance to antibiotics is a critical issue that has increased the demand for novel antibacterial compounds. Antibacterial activities have been evaluated in extracts from photosynthetic green microalgae, with varying levels of subsequent potential for development based on the strain of algae, strain of bacterial pathogen, and solvent used to extract the metabolites. Green microalgae from extreme environmental conditions have had to adapt to conditions that exclude many other organisms. The production of antibacterial compounds aids directly or indirectly in the survival of green microalgae in these extreme environments, as well as potentially serve other roles. This review investigates antibacterial activities of green microalgae from both extreme in-situ environmental conditions and induced extreme laboratory conditions and highlights.

• 한국기계가공학회지
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• 제21권5호
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• pp.105-109
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• 2022

In the field of medical and marine industries, antibacterial surfaces have been emerged as one of the most important issues. Recently, many researchers have been studying antibacterial surfaces to kill bacteria or prevent the adhesion of bacteria. In their researches, various materials and structures are suggested to inhibit the adhesion of bacteria or kill the attached bacteria. However, chemical materials such as antibiotics or metal could be toxic. Moreover, frequent use of antibiotics causes super bacteria having resistance to antibiotics. In this study, nano-pillar structured surface was fabricated using polyurethane acrylate (PUA) and the mechanically induced antibacterial function was confirmed based on the fabricated nanostructures. Nanostructures can damage the bacterial membrane of Gram-negative bacteria through stretching of bacterial membrane via interaction with the nanostructures and the bacterial membrane. Consequently, the proposed transparent, flexible and nanostructured PUA films can be one of promising candidates for antifouling and antibacterial surfaces which can be applied in various industries.