• Restorative Dentistry and Endodontics
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• v.25 no.4
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• pp.561-574
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• 2000

Poly-P has been used to prevent decomposition of foods and has been shown to have inhibitory effect on the growth of gram positive bacteria. The purpose of this study was to evaluate the effect of poly-P on the growth of Porphyromonas endodontalis, a gram negative obligate anaerobic rod, endodontopathic bacterium. P. endodontalis ATCC 35406 was in BHI broth containing hemin and vitamin K with or without poly-P. Inhibitory effect of each poly-P which was added at the beginning(lag phase) or during(exponential phase) the culture, MIC(minimum inhibitory concentration) was determined by measuring the optical density of the bacterial cell at 540nm. Viable cell counts were measured to determined whether poly-P has a bactericidal effect. Leakage of intracellular nucleotides from P. endodontalis was determined at 260nm and morphological change of P. endodontalis was observed under the TEM(transmission electron microscope). Binding of 32P-labeled poly-P to P. endodontalis was examined. SDS-polyacrylamide gel electrophoresis and zymography were performed to observe the changes in protein and enzyme profiles of P. endodontalis, respectively. The results from this study were as follows : 1. The minimal inhibitory concentration(MIC) of poly-P to P. endodontalis appeared to be 0.04~0.05%. 2. Poly-P added to the P. endodontalis culture during the exponential phase of P. endodontalis was as much effective as poly-P added at the begining of the culture, suggesting that the antibacterial effect of poly-P is not much dependent on the initial inoculum size of P. endodontalis. 3. Poly-P are bactericidal to P. endodontalis, demonstrating the decrease of the viable cell counts. 4. Intracellular nucleotide release from the P. endodontalis, was not increased in the presence of poly-P and was not reversed by the addition of divalent cations like $Ca^{2+}$ and $Mg^{2-}$. 5. Under the TEM, it was observed that fine electro-dense materials were prominent in the poly-P grown P. endodontalis, appearing locally in the cell, and the materials were more abundant and more dispersed in the cell as the incubation time with poly-P increased. In addition, highly electron dense granules accumulated in many poly-P grown cells, most of which were atypical in their shape. 6. Binding of 32P-labeled poly-P to P. endodontalis appeared to be 32.8 and 45.5 and 53.4% at 30 minutes, 1 hours and 2 hours, respectively. 7. In the presence of poly-P. the synthesis of proteins with apparent molecular masses of 25, 27, 35, 45 was lost or drastically decreased whereas expression of a protein with an apparent molecular mass of 75 was elevated. 8. Proteolytic activity of P. endodontalis was decreased by poly-P. The overall results suggest that use of poly-P may affect the growth of P. endodontalis, and the anti-bacterial activity of poly-P seems largely bactericidal. Changes in shape, protein expression, and proteolytic activity of P. endodontalis by poly-P may be directly and indirectly attributed to the antibacterial effect of poly-P. Further studies will be needed to confirm the effect of poly-P.

• Journal of Life Science
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• v.28 no.6
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• pp.688-696
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• 2018

Hemp seed (Cannabis sativa L.; HS), an annual herbaceous plant in the Cannabis genus, has been reported to play various biological functions in immunity increase, atherosclerosis, constipation, hyperlipidemia prevention, anti-inflammatory, and anti-cancer. In recently years, as superfood, the growing interest in the health care benefits of hemp seed has led to increased consumption. In this study, we investigated the effect of an ethanol extract of HS fermented with lactic acid bacteria (Lactobacillus plantarum KCTC 3107, L. plantarum KCTC 3108, L. brevis BHN-LAB128, L. paracasei BHN-LAB129). An antibacterial activity against Staphylococcus aureus and Bacillus cereus were 13.99 mm and 15.17 mm, respectively. The ethanol extracts of fermented hemp seed by lactic acid bacteria that the contents of total polyphenol, total flavonoid content, DPPH radical scavenging activity, SOD-like activity, and ${\alpha}$-glucosidase inhibitory activity were increased compared to non-fermented hemp seed. Also, tyrosinase inhibitory activity of the fermented hemp seed (FHS), known to melanin increasing substance was increased. In these results, we suggested that FHS have effects of anti-oxidant, ${\alpha}$-glucosidase inhibitory activity, and tyrosinase inhibitory activity. Hence, we proposed that FHS has possible to development as functional foods and cosmetics.

• Bulletin of the Korean Chemical Society
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• v.29 no.6
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• pp.1179-1184
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• 2008

Commercial nanometer sized silver is widely used for its antibacterial effect; however, nanoparticles may also have ecotoxicological effects after being discharged into water. Nanometer sized silver can flow into aquatic environments, where it can exert a variety of physiologically effects in living organisms, including fish. The present study aimed to investigate the effect of nanometer sized silver on the development of zebrafish embryos, analyze the properties of commercial nanometer sized silver and define the toxicity relationship between embryogenesis and hatched flies. The commercial nanometer sized silver was analyzed in the $Ag^+$ ion form. The hatch rate decreased in the nano-silver exposed groups (10 and 20 ppt); furthermore, the hatched flies had an abnormal notochord, weak heart beat, damaged eyes and curved tail. The expression of the Sel N1 gene decreased in the nano-silver exposed groups, and the catalase activities of the exposed groups increased relative to those in the control group. Therefore, the $Ag^+$ ions in commercial nanometer sized silver could accumulate in aquatic environments and seriously damage the development of zebrafish embryos.

• Textile Coloration and Finishing
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• v.28 no.1
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• pp.14-22
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• 2016

The objective of this study is to develop eco-friendly, functional towel material utilizing cotton-Modal-chitosan blended(C-M-CH) yarn and natural dyeing with mugwort colorants. Dyeing properties of towels with mugwort colorants were studied by investigating the effect of dyeing conditions including concentration of mugwort colorants, dyeing temperature, and dyeing time, and the effects of mordants on dye uptakes were investigated. The C-M-CH towel showed better dye uptake than 100% cotton towel with mugwort colorants. The shade of towels got darker and red-yellowish tint increased by mordanting. Comparing with 100% cotton towel, the colorfastness of dyed C-M-CH towel was satisfactory showing above 3 grade which is the lowest grade to washing fastness. The antibacterial activity against Staphylococcus aureus and deodorization performance of towels were excellent and improved by dyeing with mugwort colorants. From the results obtained, it is concluded that the cotton-Modal-chitosan blended towel dyed with mugwort colorants can be used practically for an eco-friendly and multi-functional towel materials with excellent absorbance and drying properties.

• Proceedings of the Korea Concrete Institute Conference
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• 2006.05b
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• pp.593-596
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• 2006

The environment-friendly building material, photocatalyst/scoria/loess concrete, was prepared using scoria and loess (which have merits as building materials) and photocatalyst (which has the functions to compose the environmental contaminants and of self cleaning). In order to apply this material as a building material, the compressive and flexible strengths, and water absorptivity (which have been set by Korea Industrial Standard) were measured. In order to know the environment-friendly characteristics of this material, several tests, such as, the tests of emissivity and emission power of far infrared ray and acoustic absorptivity, antibacterial test for Escherichia coli and Pseudomonas aeruginosa, antifungal test for mixed fungal strains, and deodorization test of ammonia were carried out. Moreover, the removal characteristics of NOx, and formaldehyde (HCHO) by photocatalyst/scoria/loess concrete were examined as the following different parameters: the removal characteristics of these contaminants with the substitution ratio and the kind of photocatalyst, light source, UV intensity of sunlight, relative humidity, intial NOx concentration.

• The Journal of the Korean dental association
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• v.10 no.1
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• pp.35-40
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• 1972

저자는 임상에서 널리 사용되는 근관충전재료인 산화아연유지놀, N2, MN2 및 Triozinc Paste의 살균효과를 비교하기 위하여 한천배양기 및 혈액가한천배양기상에서 백색포도상구균 및 α-용혈성연질상구균의 두균주를 사용, 실험하여 다음과 같은 결과를 얻었다. 1) 백색포도상구균을 심은 한천배양기상에서 비건조도판을 사용한 실험군에서의 균성장억제대는 MN2, 6. 83; Triozinc paste, 5. 77; N 2, 5. 61; 산화아연유지놀, 3.49mm였다. 2) 백색포도상구균을 심은 한천배양기상에서 건조도판을 사용한 실험군에서의 균성장억제대는 MN2, 4.40; Triozinc paste, 1.46; 산화아연유지놀, 0.99; ㅜ2, 0.84mm였다. 3) 백색포도상구균을 심은 혈액가한천배양기상에서 건조도판을 사용한 실험군에서의 균성장억제대는 MN 2, 1.15; Triozinc paste, 0.55; 산화아연유지놀, 0.54; N2, 0.43mm였다. 4) α-용혈성연질상구균을 심은 용혈가한천배양기상에서 건조도판을 사용한 실험군에서의 균성장억제대는 MN 2, 2.14; Triozinc paste, 1.00; 산화아연유지놀, 0.75; N 2, 0.43mm였다.

• The Journal of Korean Medical History
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• v.26 no.2
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• pp.135-148
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• 2013

This study analyzed surface-chemical transitions in manufacturing process of traditional acupuncture as proposed method by Dongeuibogam. The manufacturing process of traditional acupuncture from a used iron for a long time was divided by primary medicinal herbs treatment, secondary medicinal herbs treatment and tertiary treatment using by dog meat. The traditional acupuncture research process was measured according to the characteristics and changes of the specimens at each processing step of the manufacturing process. The following devices were used to Surface analysis. Scanning electron microscopy (SEM), Energy Dispersive Spectrometer (EDS), X-ray diffraction analyzer (XRD). As a result, medicinal herbs removed impurities on the surface and raised the antibacterial effect in the manufacturing process of traditional acupuncture. Furthermore, the ingredients of medicinal herbs were coated on the surface of the iron. Dog meat influenced to prevent surface corrosion, reduce friction when the acupuncture was inserted. Although the process empirically obtained, a glimpse of the wisdom of our ancestors was revealed. These ancestral wisdom can be expected to apply today, when used in manufacturing process of a modern stainless steel acupuncture to compensate for the defective part.

• Korean Journal of Human Ecology
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• v.18 no.3
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• pp.749-756
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• 2009

Keeping abreast with the latest consumer's trends, industries are focusing on sensibility aspects of products to meet consumer's needs. The car(?) seat cover fabrics are more closely related to human senses than anything else. This study attempted to investigate which seat cover fabric can give good feeling to consumers and to analyze their characteristics. Twelve kinds of jacquard fabric used for seat cover were selected. The Kawabata Evaluation System was used to measure the mechanical properties of 12 jacquard fabrics, and tactile sensibility(TS), and preference(P) determined by subjective evaluation of 160 participants were also utilized. The stepwise regression analysis was made to select the most significant mechanical properties, and some models for predicting tactile sensibility and preference was developed. The results are briefly summarized as follows: the most important parameter to choose seat cover fabric is a "hygienic property" and the other parameters are 'materials with color fastness', 'compressive property', 'color', 'antibacterial property', 'easy-care property'. The LogSMD, LogB, LC, EM were selected as significant mechanical properties affecting tactile sensibility. Also, the LC, LogB, LogSMD, LogWC, LogMMD were selected as significant mechanical properties affecting preference.

• Food Science and Biotechnology
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• v.16 no.4
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• pp.537-542
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• 2007

Sophora flavescens AITON (Leguminosae) is a typical traditional Korean medical herb considered to exhibit antibacterial, anti-inflammatory, and antipyretic effects, and is also used for the treatment of skin and mucosal ulcers, sores, diarrhea, gastrointestinal hemorrhage, arrhythmia, and eczema. In this study, the compound sophoraflavanone G was isolated from the dried roots of S. flavescens by bioassay-guided fractionation. We then investigated the effects of various concentrations of sophoraflavanone G on cell viability and the induction of apoptosis in KB cells after an incubation of 24 hr. The results were determined by the following methods: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-terazolium bromide (MTT) assay, Hoechst-33258 dye staining, flow cytometry (cell cycle), and Western blotting for caspase-3 and poly (ADP-ribose) polymerase (PARP). We found sophoraflavanone G induced the apoptosis of KB cells in a dose-dependent manner that was verified by DNA fragmentation, apoptotic bodies, the sub-G1 ratio, caspase-3 activity, and cleavage of PARP. These results suggest that sophoraflavanone G has potent anti-proliferative effects on human oral epidermoid carcinoma cells, with the induction of apoptosis.

• Journal of the Korean Society of Safety
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• v.7 no.3
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• pp.22-29
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• 1992

Emulsion-type cometics contain many kinds of carbon and energy source i.e., vegetable oil, mineral oil and carbohydrate etc., those can be used as nutrients and caused contamination by microbials. Thereby we have to keep cosmetics from the possibility of contamination by microbials. From this viewpoint, the purpose of this study is to get the data necessary not only to prevent dermatopathia occurred by microbials but also to sustain the quality. In this experiment, we observed how many Staphylococcus aureus were grown in the prepared cosmetics with or without antiseptics so as to prevent contamination. When the contamination proceed, the stability of phase was disturbed and creaming phenomina was happened with some discoloration and bad smell. About 40 days after, the pH was changed from 7.6 to 6.5 and the refractive index of cosmetic raw materials were changed from 1.4415 to 1.4490(water : oil=70:30). By adding antiseptics Into prepared cosmetics, the number of Staphylococcus aureus with challenge test method were decreased to 7$\times$103 cell/ml. For the antibacterial effect against Staphylococcus aureus, p-hydroxy benzoic acid propyl ester in phosphoric acid buffer solution was the best.