• YAKHAK HOEJI
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• v.36 no.4
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• pp.357-369
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• 1992

Actions for 48-hour homolgous passive cutaneous anaphylaxis (48-hr PCA) and chemical mediators were investigated in mice and rats. The hyaluronidase activity, which was used in the in vitro screening test of the antiallergic action, was significantly inhibited by Magnoiliae Flos, Achyranthis Radix, Forsythiae Fructus, Alpiniae Fructus, Anemarrhenae Rhizoma and Ponciri Fructus among twelve medicinal plants and tranilast as a comparative drug of the antiallergic action. In the mouse ear, 48-hr PCA was significantly inhibited by intraperitoneal (i.p.) pretreatment with Magnoliae Flos, Achyranthis Radix, Alpiniae Fructus, Anemarrhenae Rhizoma, Ponciri Fructus, Ledebouriellae Radix and tranilast. And also, the increment of vascular permeability induced by histamine or serotoin was inhibited significantly by i.p. pretreatment with Magnoliae Flos, Achyranthis Radix, Alpiniae Fructus, Anemarrheuae rhizoma, Zizyphi Fructus and tranilast. In the rat dorsal skin, the increment of vascular permeability induced by histamine or serotonin was significantly inhibited by i.p. pretreatment with Magnoliae Flos, Acyranthis Radix, Alpiniae Fructus, Anemarrhenae Rhizoma and tranilast. And also, the increment of vascular permeability induced by compound 48/80 or calcium ionophore A 23187 was significantly inhibited by i.p. pretreatment with Magnoliae Flos, Achyranthis Radix, Alpiniae Fructus, Amemarrhenae Rhizoma, Zizyphi Fructus, Ledebouriellae Radix, Lithospermi Radix and tranilast. These results suggest that each water extracts of Magnoliae Flos, Achyranthis Radix, Alpiniae Fructus and Anemarrhenae Rhizoma have especially antiallergic activities.

• Advances in Traditional Medicine
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• v.1 no.1
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• pp.1-7
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• 2000

Although Oriental medicines have long been used effectively in treating many diseases throughout the world, the pharmacological mechanisms of most Oriental medicines used have not been defined. As part of our continuing search for biologically active antiallergic drugs from natural sources, Oriental medicines were analyzed. Some Oriental medicines have been used against various allergic diseases for generations, and still occupies an important place in traditional medicine in Korea. It is also still unclear how Oriental medicine prevents allergic disease in experimental animal models. Some Korean folk medicines inhibited the mast cell-mediated allergic reaction. This review summarizes the effective folk medicine in experimental effect of allergic reaction. Potential antiallergic folk medicines include: Poncirus trifoliata; Siegesbeckia glabrescence; Solanum lyratum; Aquilaria agallocha; Ulmi radicis; Polygonum tinctorium; Hwanglyun-Haedok-Tang; Rehmannia glutinosa; Kum- Hwag-San; Syzygium aromaticm; Spirulina platensis; Sosiho-Tang; Sinomenium acutum; Schizonepta tenuifolia; Shini-San; Magnoliae flos; Sochungryoung-Tang; Oryza sativa; Cryptotympana atrata; Salviae radix; Rosa davurica; Asiasari radix; Chung-Dae-San; and Cichorium intybus. Understanding the mechanisms of action for these Oriental medicines can permit drug development and laying of the ground-work for evaluating potential synergistic effects by addition and subtraction of prescriptions.

• The Journal of Korean Medicine
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• v.19 no.2
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• pp.86-99
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• 1998

Antiallergic action and the effect on normal immune function of an oriental medical prescription Gegangjochohwangsinbutang (GJHB) water extract were examined in several experimental models. GJHB water extract at 600-1,800 mg/kg/day administered orally for one week significantly recuded the passive cutaneous anaphylaxis in rats in a dose-dependent manner. Whereas the extravasation induced by intradermal leukotriene D4 in rats was significantly inhibited by GJHB water extract at 600-1,800 mg/kg/day, po, for one week, but that by histamine or serotonin was not influenced. In a rat asthma model, the delayed airway resistance in ovalbumin-induced asthmatic response was significantly attenuated by GJHB water extract of 1,800 mg/kg/day, po, administered for one week. It was also found that GJHB water extract at 1 mg/kg concentration in vitro relaxed the isolated uinea pig trachea contracted by leukotrien D4, but not that by histamine. The above findings indicate that GJHB water extract can ameliorate the adverse effect of type Ⅰ allergy such as asthma. This pharmacological action seems to derive from an inhibition of GJHB water extract on leukotriene D4-related physiological change.

• The Journal of Internal Korean Medicine
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• v.21 no.1
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• pp.156-161
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• 2000

The unripe fruit of Poncirus trifoliata Raf has been used for the treatment of allergic disease. Recently it was reported that the fruit inhibits passive cutaneous anaphylaxis and histamine release at mast cell. Type I immediate hypersensitivity of anaphylactic type is caused by released mediate chemical at mast cell. Histamine is also known as one of potent mediate chemical. Also release of mediate chemical is affected by specific stimulation of IgE combined with mast cell. Activation of mast cell is known to be stimulated by compound 48/80 and inhibited by increase of cAMP. In this experiment, the effect of water extract of Poncirus trifoliata Raf fruit (PT) on a histamine release, cAMP concentration and IgE production was measured. Compound 48/80 was administrated to the mouse peritoneal cell which was pretreated with PT. PT dosedependently inhibited histamine release at peritoneal mast cell and the serum level of histamine induced by compound 48/80. PT also instantly increased cAMP level of peritoneal mast cell right after it was added and the level gradually decreased. Production of IgE induced by antigens at mouse peritoneal cell was inhibited by PT. The IgE synthesis is induced by IL-4 and it is known that lipopolysaccharide(LPS) plus IL-4 cause an increase in IgE secretion by murine B cells. The effects of PT inhibited the production of IgE activated by LPS plus IL-4 at human U266B1 cells. These results indicate that PT has antiallergic activity by Inhibition of IgE production from B cells and histamine release by increase of cAMP.

• Korean Journal of Pharmacognosy
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• v.44 no.3
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• pp.286-290
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• 2013

This study was carried out to the determine the effects of the water extract of Viticis Fructus (Verbenaceae, WEVF) on experimental allergic reactions and inflammation. WEVF was anally administered to mice for high and fast absorption. WEVF inhibited compound 48/80-induced systemic anaphylaxis and immunoglobulin E (IgE)-mediated local allergic reaction. Histamine releasing from mast cells was reduced by WEVF, which was mediated by modulation of intracellular calcium. In addition, WEVF decreased the gene expression of pro-inflammatory cytokines in phorbol 12-myristate 13-acetate plus calcium ionophore A23187 (PMACI)-stimulated HMC-1 cells. These findings demonstrate that the WEVF possesses antiallergic and anti-inflammatory activities, which may be mediated by reducing the release of mediators such as histamine from mast cells and weakening the inflammatory action of these mediators.

• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.6
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• pp.1659-1665
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• 2005

Gamipaidok-san(GPDS) is the Hyungbangpaidok-san prescription fortified with the additional ingredients known to be effective for dermatitis. So it has been used for atopic dermatitis in the clinic work actually. In this study, we investigated the effects of GPDS on in vitro and in vivo anti-allergic effect on RBL-2H3 rat basophilic leukemia Cells and on IgE-induced passive cutaneous anaphylaxis (PCA) in mice. The in vitro anti-inflammatory activity of GPDS in RAW 264.7 cells was investigated. GPDS potently inhibited $\beta$-hexosaminidase release from RBL-2H3 and the IgE-mediated PCA reaction in mice. GPDS inhibited LPS-induced NO and PGE2 production in a dose-dependent manner Furthermore, It also inhibited inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein expression in RAW 264.7 cells, and the activation of the transcription factor, NF-kB, in nuclear fraction. The antiallergic action of GPDS may originate from anti-inflammatory activities, and can improve the inflammation caused by allergies.

• YAKHAK HOEJI
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• v.41 no.5
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• pp.652-657
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• 1997

We studied the effects of sulfasalazine(SSZ) on anaphylaxis. SSZ was found to exhibit a inhibitory activity on the compound 48/80-induced anaphylaxis. SSZ also inhibited local a naphylaxis activated by anti-dinitrophenyl(DNP) IgE. Moreover, SSZ dose-dependently inhibited histamine ralease in rat peritoneal mast cells activated by compound 48/80 or anti DNP IgE. We investigated the effects of SSZ on cAMP of rat peritoneal mast cell. The level of cAMP in rat peritoneal mast cells, when SSZ was added, transiently and significantly increased approximately 16-fold compared with that of basal cells. These results suggest that the antianaphylactic action of SSZ may be associated with an increase in the intracellular cAMP content of the mast cells as the result of an inhibition of the cAMP phosphodiesterase.

• Journal of Ginseng Research
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• v.42 no.3
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• pp.255-263
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• 2018

Orally administered ginsengs come in contact with the gut microbiota, and their hydrophilic constituents, such as ginsenosides, are metabolized to hydrophobic compounds by gastric juice and gut microbiota: protopanxadiol-type ginsenosides are mainly transformed into compound K and ginsenoside Rh2; protopanaxatriol-type ginsenosides to ginsenoside Rh1 and protopanaxatriol, and ocotillol-type ginsenosides to ocotillol. Although this metabolizing activity varies between individuals, the metabolism of ginsenosides to compound K by gut microbiota in individuals treated with ginseng is proportional to the area under the blood concentration curve for compound K in their blood samples. These metabolites such as compound K exhibit potent pharmacological effects, such as antitumor, anti-inflammatory, antidiabetic, antiallergic, and neuroprotective effects compared with the parent ginsenosides, such as Rb1, Rb2, and Re. Therefore, to monitor the potent pharmacological effects of ginseng, a novel probiotic fermentation technology has been developed to produce absorbable and bioactive metabolites. Based on these findings, it is concluded that gut microbiota play an important role in the pharmacological action of orally administered ginseng, and probiotics that can replace gut microbiota can be used in the development of beneficial and bioactive ginsengs.

• Journal of Ginseng Research
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• v.42 no.3
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• pp.264-269
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• 2018

Panax ginseng, also called Asian or Korean ginseng, has long been traditionally used in Korea and China to treat various diseases. The major active ingredients of P. ginseng are ginsenosides, which have been shown to have a variety of therapeutic effects, including antioxidation, anti-inflammatory, vasorelaxation, antiallergic, antidiabetic, and anticancer. To date, approximately 40 ginsenoside components have been reported. Current research is concentrating on using a single ginseng compound, one of the ginsenosides, instead of the total ginseng compounds, to determine the mechanisms of ginseng and ginsenosides. Recent in vitro and in vivo results show that ginseng has beneficial effects on cardiac and vascular diseases through efficacy, including antioxidation, control of vasomotor function, modulation of ion channels and signal transduction, improvement of lipid profiles, adjustment of blood pressure, improvement in cardiac function, and reduction in platelet adhesion. This review aims to provide valuable information on the traditional uses of ginseng and ginsenosides, their therapeutic applications in animal models and humans, and the pharmacological action of ginseng and ginsenosides.

• The Korea Journal of Herbology
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• v.27 no.5
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• pp.37-44
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• 2012

Objectives : The present study was undertaken to determine whether the ethanol extact of Citri Pericarpium (CP, Pericarp of Citrus unshiu Markovich, Rutaceae) is effective against atopic dermatitis according to it's storage period. Methods : To evaluate antiallergic effect of CP and OCP (Old Citri Pericarpium) evaluated in vivo their inhibitory effects against passive cutaenous anaphylaxis (PCA) reaction induced by IgE-antigen complex and scratching behaviors by compound 48/80. The Anti-atopic effects were measured by contact dermatitis, prurient animal model and PCA reaction. Contact dermatitis in mice as a model of the Type IV reaction caused by Oxazolone. Results : The results showed that anti-pruritus effects, analgesic effects of CP was depends on its hesperetin contents. And It also showed that keep longer in storage appeared to be higher in hesperetin contents. Both CP and OCP(Old CP) have a dose-dependent analgesic action in acetic acid induced writhing syndrome. OCP Potently inhibited PCA reaction in mice, although OCP weakly inhibited in long term contact dermatitis model in mice. Conclusions : These results suggest that the Proportional to the storage period, Citri Pericarpium possesses analgesic effects and anti-allergic effects.