• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2005년도 춘계학술대회
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• pp.151-185
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• 2005

• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book I
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• pp.611-616
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• 2003

Korean ginseng(Panax Ginseng C.A. Meyer) known as a oriental miracle drug is an important medicinal plant. Ginseng has been used for geriatric, tonic, stomachic, and aphrodisiac treatments for thousands years. Also, it is an antibiotic and has therapeutic properties against stress and cancer. Ginseng is widely distributed all over the world. Among them, Korean mountain ginseng has the most valuable effect on pharmaceuticals. The roots of mountain ginseng contained several kinds of ginsenosides that have many active functions for the human body. However, the study of mountain ginseng has a limit because the mountain ginseng is very expensive and rare. So, we artificially cultured mountain ginseng adventitious roots using the bioreactor culture system. We induced callus from original mountain ginseng, directly dug up in mountain and aged about one hundred ten years. Separated adventitious roots were precultured in 500ml conical flasks and then, transferred in 20L bioreactors. The adventitious roots of mountain ginseng were harvested after culturing for 40days, dried and then, extracted with several solvents. In this study, we investigated the whitening effect, anti-wrinkle effect and the safety of tissue cultured adventitious roots extract of mountain ginseng in order to identify the merit as a cosmetic ingredient. Particularly, extract of mountain ginseng adventitious roots showed whitening and anti-wrinkle effects. The inhibitory effect of this extract on the melanogenesis was examined using B-16 melanoma cell. When B-16 melanoma cells were cultured with adventitious root extract, there was a dramatically decrease in melanin contents of 8-16 melanoma cell. And we identified this extract inhibited Dopa auto-oxidation significantly. Also, when transformed mouse fibroblast L929 cells were treated with this extract, there was a significant increase in collagen synthesis. The results show significant inhibited melanization and wrinkle without inhibiting cell viability.

• 한방안이비인후피부과학회지
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• 제25권3호
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• pp.150-159
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• 2012

Objective : The purpose of this study was to evaluate the effect of korean herbal cosmetics for facial skin elasticity. Methods : We recruited patients and divided them to cosmetics group and control group randomly. We provided korean herbal cosmetics to cosmetics group and subjects in cosmetics group applied the provided cosmetics for 4 weeks. Control group waited and applied cosmetics that had previously used for 4 weeks. We assessed all subjects in 0 week, 2 weeks and 4 weeks. Primary assessment instrument was moire topography photo scale. Second assessment instrument was skin moisture, oiliness and elasticity which assessed with skintouch system v1.0, detox/regeneration questionnaire score and self-evaluating facial wrinkle/looseness score. Result : Moire topography photo scale improved in cosmetics group than control group significantly. Skin moisture and elasticity assessed with skintouch system improved in cosmetics group than control group significantly while skin oiliness assessed with skintouch system have not improved significantly. Detox/regeneration questionnaire score and self-evaluating facial wrinkle/looseness score improved in cosmetics group than control group significantly. Conclusion : Korean herbal cosmetics application might be effective method for facial skin elasticity.

• 대한한방피부미용학회지
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• 제1권1호
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• pp.198-228
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• 2005

Oriental cosmetics make the skin radiant by supplement and circulate qihyul(氣血). Oriental cosmetics aim at the radiant skin based on inner health. It accords the new trend of 21th century, well-being and naturalism. Recently a lot of new oriental cosmetics have been developed and enjoyed great popularity. The research of oriental cosmetics elucidated the active compounds having whitening effect and wrinkle cure effect. And the anti-acne and anti-atopy cosmetics have been researched. The development of oriental cosmetics should be based on oriental medical theory.

• 동의생리병리학회지
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• 제31권6호
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• pp.380-384
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• 2017

From March 1, 2017 to May 31, 2017, subjects were allowed to apply a wrinkle-improving herbal cosmetic product containing the extract of Douchi for 8 weeks. The effectiveness of herbal cosmetics was evaluated through wrinkle improvement efficacy parameter analysis, subjective wrinkle improvement through questionnaires, and adverse reaction. The herbal cosmetic products containing the extract of Douchi showed a significant decrease in the wrinkle improvement efficacy parameter analysis result. The results of the subjective satisfaction analysis after use were also evaluated positively. We could confirm the possibility of wrinkle improvement effect of Douchi extract, and it will be possible to contribute to the expansion of korean medicine field through more studies in the future.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2007년도 Proceedings of The Convention
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• pp.93-114
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• 2007

Vaccinium uliginosum L. (VU) is a flowering plant in the genus Vaccinium has berry fruit. This study was performed to investigate the effect of water extract of Vaccinium uliginosum L. and fractions on inhibition of melanogenesis and wrinkle formation. One hundred grams of the Vaccinium uliginosum L. was extracted with 2,000 mL of water ($90^{\circ}C$, 16h, 2 times). The water extracts were lyophilized and stored at $4^{\circ}C$ until used. Vaccinium uliginosum L. extracts showed scavenger activities on DPPH radical, superoxide anion radical, hydroxyl radical, hydrogen peroxide and singlet oxygen radical, dose dependently. And VU extract and fractions reduced melanin contents on B16F10 melanoma and inhibited the expression of melanogenesis-related proteins, tyrosinase, tyrosinase-related protein (TRP-1) and dapachrometa utomerase (Dct, TRP-2). Moreover VU extract and fractions stimulated procollagen production and inhibited MMP-1 production in human fibroblast. And it decreased degree of wrinkle formation in hairless mouse skin that induced by DVB irradiation for 9 weeks. From the above results, it is possible that Vaccinium uliginosum L. may be developed to be the health functional food and functional cosmetics that have anti-melanogenesis and anti-wrinkle effect.

• 한국미생물·생명공학회지
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• 제37권4호
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• pp.399-404
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• 2009

재조합 대장균으로부터 회수된 고순도 베타카로틴을 첨가한 기능성 화장품의 제조방법을 개발하고 이의 임상실험을 수행하여 개발된 화장품의 주름개선 성능을 평가하였다. 베타카로틴의 유효성, 각종 피부흡수 및 사용성, 질감 등을 고려하여 화장품을 처방하였다. 베타카로틴의 첨가량은 피부에 독성을 나타내지 않고 콜라겐생합성 촉진효과가 우수하며 베타카로틴 자체의 특유한 붉은 색소가 피부에 묻어나오지 않을 정도인 화장품 kg 당 1 mg으로 결정하였다. 베타카로틴을 용해시키기 위하여 식용오일을 사용하였고 항산화물질인 vitamin E(tocopheryl acetate)를 첨가함으로써 제품의 장기간 보관 시 베타카로틴의 안정성과 유효성에 도움을 주게 하였다. 유화제 및 유화안정제는 2.0% 첨가함으로써 유상과 수상이 분리되지 않도록 하였다. 유연제인 dimethicone을 0.35% 첨가함으로써 백탁 현상을 제거하고 피부에 대한 사용감을 좋게 하였다. 보습작용을 보이는 sorbitol을 첨가함으로써, 건조한 환경에 대한 피부트러블을 최소화하게 하였다. 제품의 안정도를 조사하였다. 60일간의 실험에서 상온에 보관된 제품의 pH는 변화가 거의 없었고 0, 25, $40^{\circ}C$의 온도서 물리 화학적 변화가 없는 안정한 상태를 유지하였다. 뿐만 아니라 광제품의 주름개선 성능을 평가하기 위하여 20명의 피시험자를 대상으로 화장품의 인체적용시험을 실시하였다. 시험자의 평가결과 4주 후 피험자의 38%가 주름이 감소하였고 8주 후에는 68%가 주름이 개선되었다.

• Journal of Pharmaceutical Investigation
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• 제29권4호
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• pp.279-285
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• 1999

Retinoids in anti-wrinkle cosmetics are widely used for the treatment of the photoaging skin these days, but very unstable in the presence of water. Multilamellar liquid crystal vesicles consisted of $poly(oxyethylene)_{15}oleyl$ ether $(POE_{15}OE)$ or $poly(oxyethylene)_{15}stearyl$ ether $(POE_{15}SE)$, cetostearyl alcohol (CSA) and cholesterol or oils were prepared to increase the stability of retinyl acetate or retinyl palmitate. $POE_{15}SE-CSA-Water$ systems were more unstable than $POE_{15}SE-CSA-Water$ systems due to the bended structure of oleyl groups. For the modification of vesicles, cholesterol was added. The enthalpy values were decreased reaching to zero and the water permeability of systems was decreased, but the stabilizaton of retinyl acetate was not greatly improved. The stability of retinyl acetate may depend on its location in the structure. Various oils were added for further stabilization and isopropyl myristate was most effective. In practice, retinyl palmitate showed better stability in the same vesicle than retinyl acetate, and the improved vesicles could be used for the anti-wrinkle cosmetics

• 대한화장품학회지
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• 제30권4호
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• pp.449-456
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• 2004

최근 홍화씨의 세로토닌유도체, 리그난 및 플라보노이드와 같은 화합물은 에스트로겐${\cdot}$항에스트로겐 활성을 갖는 식물 유래의 diphenol 화합물인 phytoestrogen 물질로 작용할 수 있음이 밝혀지면서 이를 함유하고 있는 홍화씨추출물[safflower (Carthamus tinctorius L.) seed extract]의 피부주름 개선효과가 기대되고 있다. 본 연구진은 이전에 홍화씨추출물은 피부세포의 산화를 막아주는 항산화작용을 지니고 있으며, 아울러 피부의 matrix protein을 만들어내는 섬유아세포 증식을 촉진할 뿐만 아니라 피부의 구조를 이루고 있는 엘라스틴 단백질을 분해하는 elastase의 활성 저해효과를 보고한 바 있다. 이러한 연구 배경에서 본 연구에서는 홍화씨추출물의 피부주름 개선효과를 알아보기 위해 콜라겐 발현효과 및 MMP-1 발현 억제효과를 각각 측정하였다. 그 결과 홍화씨추출물의 콜라겐 발현효과는 레티놀보다 우수하였으며, 아울러 MMP-1 발현 저해효과는 올레산과 유사하게 농도 의존적으로 저해되었다. 또한 $0.1\%$ 홍화씨추출물 함유 크림은 대조군(홍화씨추출물 미함유 크림)에 비하여 피부주름 개선 효과가 크게 나타났다. 이러한 결과를 볼 때 홍화씨추출물은 주름개선용 기능성화장품 신소재로의 사용이 기대된다.

• 대한화장품학회지
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• 제31권3호
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• pp.213-218
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• 2005

최근 노령화 인구의 증가와 다양한 피부미용 기술들이 증대됨에 따라 피부노화 개선에 대한 소비자의 욕구가 높아지고 있다. 또한 기능성 화장품법 시행 후 효능원료 개발에 대한 연구들이 활발히 수행되고 있어 안전하며 임상학적으로 효능이 있는 주름기능성 화장품 개발은 화장품업계의 주요 관심 과제이다. 대표적인 피부 고민들 가운데 주름살 증가와 탄력성 감소와 같은 피부노화 현상을 개선할 수 있는 항주름 연구 및 항노화 원료들에 대한 연구 동향을 소개하고자 한다.