• Proceedings of the Korean Institute of Building Construction Conference
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• 2016.10a
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• pp.150-151
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• 2016

In previous study, researchers studied development of early anti-freezing cement at low temperature (-5℃) using hydration characteristics of reduction slag. In this study, the durability of concrete using reduction slag was conducted. The experiment result, reduction slag makes high temperature and improves compressive strength due to quick setting. And then result of durability show that it is no problem. However, it is considered that further study is needed about high shrinkages which was indicated in dry shrinkage.

• Bulletin of the Korean Chemical Society
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• v.31 no.12
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• pp.3777-3781
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• 2010

The anticancer effect and apoptotic mechanism of a novel indole derivative AC-264, a lead derived from a chemical library, were investigated in human promyelocytic leukemia HL-60 cells. HL-60 cells treated with AC-264 at various concentrations showed the morphological features of apoptosis, such as plasma membrane blebbing and cell shrinkage. AC-264 exhibited cytotoxic effect in various cancer cell lines with different degrees of potency. Especially, AC-264 was effective on increasing the population of apoptotic cells in HL-60 cells, as detected by the number of cells stained with Annexin V and PI. Furthermore, AC-264 activated caspase-3 enzyme activity and induced internucleosomal DNA fragmentation. These results indicated that AC-264 produces anti-cancer effect via apoptotic cell death by activating caspase-3 and inducing internucleosomal DNA fragmentation in HL-60 cells.

• Structural Engineering and Mechanics
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• v.75 no.4
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• pp.477-485
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• 2020

Finite element (FE) model based structural damage detection (SDD) methods play vital roles in effectively locating and quantifying structural damages. Among these methods, structural model updating should be conducted before SDD to obtain benchmark models of real structures. However, the characteristics of updating parameters are not reasonably considered in existing studies. Inspired by the l_∞ norm regularization, a novel anti-sparse representation method is proposed for structural model updating in this study. Based on sensitivity analysis, both frequencies and mode shapes are used to define an objective function at first. Then, by adding l_∞ norm penalty, an optimization problem is established for structural model updating. As a result, the optimization problem can be solved by the fast iterative shrinkage thresholding algorithm (FISTA). Moreover, comparative studies with classical regularization strategy, i.e. the l₂ norm regularization method, are conducted as well. To intuitively illustrate the effectiveness of the proposed method, a 2-DOF spring-mass model is taken as an example in numerical simulations. The updating results show that the proposed method has a good robustness to measurement noises. Finally, to further verify the applicability of the proposed method, a six-storey aluminum alloy frame is designed and fabricated in laboratory. The added mass on each storey is taken as updating parameter. The updating results provide a good agreement with the true values, which indicates that the proposed method can effectively update the model parameters with a high accuracy.

• Journal of the Korean Ceramic Society
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• v.42 no.3 s.274
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• pp.198-204
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• 2005

Fluosilicate salts based hydration heat reducer(SWP-HR), used in this study, is composed of fluosilicate salts, soluble silica, aromatic polymer condensate and nitrate salt based inorganic compound with latent heat property. Effects of SWP-HR addition on the hydration heat and anti-crack property of cement mortar were investigated. Adiabatic hydration temperature and drying shrinkage length of SWP-HR added cement mortar had a tendency to decrease compared to those of cement mortar without SWP-HR addition. Also, it was confirmed through crack pattern experiment of plate-form specimen for elucidating crack-reducing characteristic that anti-crack property of SWP-HR added cement mortar was improved.

• Journal of Life Science
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• v.29 no.2
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• pp.256-264
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• 2019

Lymphotoxin ${\beta}$ receptor ($LT{\beta}R$), a member of the tumor necrosis factor receptor family, plays an important role in lymphoid tissue's architecture and organogenesis. In contrast, MLCK and ROCK play critical roles in the regulation of stress fiber (SF) formation in cells. To determine whether $LT{\beta}R$ stimulation in fibroblastic reticular cells (FRCs) is involved in these signaling pathways, myosin light chain kinase inhibitor-7 (ML-7) was used to inhibit them. ML7-treated FRCs completely blocked SFs and showed retraction and shrinkage processes comparable to those observed in agonistic anti-$LT{\beta}R$ antibody-treated cells. The inhibition of ROCK activity with Y27632-induced changes in actin cytoskeleton organization and cell morphology in FRCs. Actin bundles rearranged into SFs, and phospho-myosin light chain (p-MLC) co-localized in FRCs. We checked the level of Rho-guanosine diphosphate (RhoGDP)/guanosine triphosphate (GTP) exchange activity using FRC lysate. When $LT{\beta}R$ was stimulated with agonistic anti-$LT{\beta}R$ antibodies, Rho-GDP/GTP exchange activity was markedly reduced. Regarding $LT{\beta}R$ signaling with a focus on MLCK inhibition, we showed that the phosphorylation of MLCs was reduced by $LT{\beta}R$ stimulation in FRCs. Cytoskeleton components, such as tubulin, b-actin, and phospho-ezrin proteins acting as membrane-cytoskeleton linkers, were produced in de-phosphorylation, and they reduced expression in agonistic anti-$LT{\beta}R$ antibody-treated FRCs. Collectively, the results suggested that MLCK and ROCK were simultaneously responsible for SF regulation triggered by $LT{\beta}R$ signaling in FRCs.

• Journal of the Korean Society of Clothing and Textiles
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• v.15 no.2 s.38
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• pp.199-209
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• 1991

In this paper, the fabrics for Korean folk clothes(KFC) undergoes repeated laundering under given condition. After this cyclic laundering was applied, the mechanical properties of the specimen were measured using KES-F system in order to evaluated the end-use performance of fabrics for KFC. And also, the crease recovery of fabrics for KFC were measured by shirley crease recovery tester. 78 different kinds of commercial silk fabrics and polyester fabrics for KFC were used for this study. The experimental results were analysed statistically to relate the mechanical properties and the crease recovery of fabrics for KFC. Furthermore, these changes in dimensional stability, mechanical properties and handle of fabrics for KFC were discussed in comparison with those values for silk fabrics and polyester fabrics. The results obtained are as follows. 1. Regardless of materials, remarkable increase are observed in shrinkage of the fabrics for KFC about repeated laundering, but dull increase are observed in shrinkage after 10 cycles of the repeated laundering. On the other hand, slack extend are observed in dimensions after 20 cycles of the repeated laundering. The shrinkage of fabrics for KFC after 10 cycles of the lundering showed that the silk fabrics are $1.74{\pm}0.33\%$ (warp direction) and $1.35{\pm}0.23\%$ (weft direction) and the polyester fabrics are $1.45{\pm}0.22\%$ (warp direction) and $1.25{\pm}0.23\%$ (weft direction). 2. Except for tensile property, these changes in mechanical properties of fabrics for KFC by laundering have $\pm$ 16 range of bending, shearing, compression, surface, thickness & weight as compared with before laundering. Particularly, the LT and RT about $1\~3$ cycles of the repeated laundering showed remarkable decrease. And SMD, WC, T & W of fabrics for KFC by the laundering were more increased than one for original fabrics. But B, 2HB, G, 2HG, 2HGS were decreased more than one for original fabrics. 3. 'Stiffness', 'Anti-drape', 'Crispness' and 'Scroop' hand values decrease and'Fullness & softness', 'Flexibility & softness' hand values increase with repeated laundering. 4. Remarkable decrease are observed in crease recoveries about $1\~5$ cycles of the repeated lundering, but slack decrease are observed in crease recoveries after 5 cycles of repeated laundering. The crease recovery of fabrics for KFC have negative(-) correlation with LT, RT, G, RC and MMD, This fact implies that the smaller these values, the larger the crease recovery. The crease recovery of fabrics for KFC has a high degree of correlation with the mechanical properties such as shearing, compression, surface property. And also, the crease recovery are expected by measuring the mechanical properties such as G, 2HG, 2HGS, RC, WC, LC, MIU, MMD and SMD, according to the obtained regression equation.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.11
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• pp.1408-1414
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• 2008

The anticancer properties of Scolopendra subspinipes mutilans extract were investigated. The extract from S. subspinipes mutilans by 80% EtOH was fractionated with n-hexane, dichloromethan ($CH_2Cl_2$), ethylacetate (EtOAc), and butanol (BuOH) in order. The EtOAc fraction showed the highest inhibitory activity (about 80%) against human leukemia (HL-60) cell growth at $50\;{\mu}g/mL$. To explore the mechanism of cytotoxicity, we used several measures of apoptosis to determine whether these processes were involved in EtOAc fraction-induced HL-60 cell death. Our results showed EtOAc fraction induced cell shrinkage, cell membrane blebbing, apoptotic body, and DNA fragmentation. The EtOAc fraction gradually decreased the expression of anti-apoptotic Bcl-xL and led to the activation of caspase-3, -9 and cleavage of PARP. These findings suggest that S. subspinipes mutilans exhibits potential anticancer properties.

• Journal of Physiology & Pathology in Korean Medicine
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• v.19 no.3
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• pp.640-646
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• 2005

This study was performed for the investigation of anticancer effects of Siegesbeckia glabrescens(SG) on HepG2 cells, a human hepatoma cell line. In the previous study, we examined the involvement of nitric oxide (NO) on anti-proliferative and apoptotic efficacy of SG in vascular smooth muscle cells. The possible mechanism of the apoptotic effects of SG was investigated in HepG2 cells. SG showed potent cytotoxic activity in HepG2 but not chang cells, liver normal cells. SG treatment caused morphological change such as cell shrinkage, nuclei condensation and cell blebbing in HepG2 cells. SG also increased the nitrite production of HepG2 cells in a dose-dependent manner. Furthermore, L-NNA treatment inhibited the anti-proliferative effect of SG. From RT-PCR, SG decreased Bcl-2 but no affected on Bax. Western blot for procaspase-3 and COX-2 showed that degradation of procaspase-3 protein level or inhibition of COX-2 protein expression by SG treatment. In addition, the apoptotic effect of SG was also demonstrated by DNA laddering. In conclusion, SG-induced HepG2 cells death can occur via apoptosis which was dose-dependent, and associated with apoptosis-related Bcl-2/Bax gene expressions, COX-2 inhibition, caspase-3 activation and NO pathway. These results suggest that SG is potentially useful as a chemotherapeutic/chemopreventive agent in hepatocellular carcinoma.

• Journal of Life Science
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• v.27 no.10
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• pp.1199-1206
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• 2017

The lymphotoxin ${\beta}$ receptor ($LT{\beta}R$), a member of the tumor necrosis factor receptor family, plays an important role in lymphoid tissue's architecture and organogenesis. We found that $LT{\beta}R$ stimulation induced changes in stress fibers (SFs) in fibroblastic reticular cells (FRCs). MLCK and ROCK play critical roles in the regulation of SF formation in cells. The present study was performed to investigate the antifibrotic effects on SF regulation of $LT{\beta}R$ signaling, with a focus on MLCK inhibition. The effect of $LT{\beta}R$ on the SF change was analyzed using immunoblot and fluorescence assays and agonistic $anti-LT{\beta}R$ antibody-treated FRCs. In addition, we checked the level of Rho-guanosine diphosphate (GDP)/guanosine triphosphate (GTP) exchange activity with FRC lysate. Phospho-ezrin proteins acting as membrane-cytoskeleton linkers completely de-phosphorylated in agonistic $anti-LT{\beta}R$ antibody-treated FRCs. The actin bundles rearranged into SFs, where phospho-myosin light chain (p-MLC) co-localized in FRCs. ML7-treated FRCs completely blocked SFs and showed retraction and shrinkage processes comparable to those observed in agonistic $anti-LT{\beta}R$ antibody-treated cells. Inhibition of ROCK activity induced changes in the actin cytoskeleton organization; however, some SFs remained in the cells, while they were completely disrupted by MLCK inhibition with ML7. We showed that the phosphorylation of MLC was completely abolished with $LT{\beta}R$ stimulation in FRCs. When $LT{\beta}R$ was stimulated with the agonistic $anti-LT{\beta}R$ antibody, the Rho-GDP/GTP exchange activity was reduced, however, the activity was not completely abolished. Collectively, the results illustrated that MLCK was potently responsible for the SF regulation triggered via $LT{\beta}R$ signaling in FRCs.

• Textile Coloration and Finishing
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• v.6 no.2
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• pp.47-54
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• 1994

Cotton fiber, NaOH-mercerized cotton fiber, cotton fabric, and NaOH-mercerized cotton fabric have been treated by liquid ammonia at -33.4$^{\circ}C$. The fine structures, bending properties, tensile strengthes, shrinkages for laundering, and wrinkle recoveries were studied. The treatment of cottons with liquid ammonia brought about the transition of crystal lattice ; transforming cellulose I crystal of original cotton to cellulose I and III crystal, and cellulose II crystal of mercerized cotton to cellulose II and III crystals. The degree of crystallinities were decreased in the order of liquid ammonia>NaOH/liquid ammonia>NaOH-treated cotton. However moisture regain and water absorbency for liquid ammonia-treated cotton were lower than that of NaOH-treated cotton because of a difference in swelling actions of the agents. It seems caused by intermicrofibrillar pores produced in swelling processes. The bending rigidity and bending hysteresis were decreased remarkly by liquid ammonia treatment. Therefore softness and dimensional stability were improved. The liquid amminia and NaOH/liquid ammonia-treated cottons moreover show excellent properties in tensile strength, anti-shrinkage for laundering, and wrinkle recovery.