• 제목/요약/키워드: anti-design

검색결과 1,176건 처리시간 0.033초

Monascus 균사체의 소규모 배양을 통한 고생산성 균주의 대규모 선별방법 확립과 통계적 생산배지 최적화를 통한 Monacolin-K 생산성 향상 (Establishment of Miniaturized Cultivation Method for Large and Rapid Screening of High-yielding Monascus Mutants, and Enhanced Production of Monacolin-K through Statistical Optimization of Production Medium)

  • 이미진;정용섭;김평현;전계택
    • KSBB Journal
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    • 제22권5호
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    • pp.305-312
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    • 2007
  • 가능한 한 대량의 균주를 테스트하여 고생산성을 지닌 균주를 신속히 선별하기 위해서는 소규모 액상배양 방법의 확립이 필수적인데, 강력한 지질저하제인 monacolin-K의 생산균주인 균사형성 Monascus의 경우 포자나 균사체의 형성이 활발하지 않은 배양 생리적 특성으로 인해 소규모 (miniature) 액상배양이 매우 어려운 문제점이 있다. 본 연구에서는 monacolin-K 고생산성 균주개량의 효율성을 큰 폭으로 증가시키고자, 기존의 플라스크 액상배양 방법을 소규모화시킨 tube 배양 방법을 개발함으로써 단기간에 보다 많은 양의 균주를 테스트하고자 하였다. 이차대사산물인 monacolin-K의 생합성은 각각의 배양단계에서의 생산균주의 배양형태가 중요한데, 특별히 최종 생산배양에서 생산성 증가에 심각한 영향을 미치는 배양형태인 직경 1 mm 이하의 pellet 모양을 유도하기 위해서는 성장배양 시에 반드시 고농도의 균사모양이 유도되어야 하는 것으로 관찰되었다. 50 ml tube (7 ml의 조업부피)를 이용하는 소규모 액상 성장배양의 경우 solid seed 배양 단계에서의 포자형성배지의 조성을 통계적 방법을 통해 최적화하고, 또한 성장배지 성분에 brown rice 가루 20 g/L를 첨가할 때, 원하는 균사모양의 배양형태가 유도됨을 확인할 수 있었다. 한편 7 ml의 조업부피의 tube를 이용한 소규모 성장배양 방법의 개발로 인해, 기존의 flask 배양을 이용할 때보다 단기간에 훨씬 많은 변이주의 생산성을 조사하게 되어 균주개량 속도를 큰 폭으로 향상시킬 수는 있었으나, 선별된 개별 균주의 monacolin-K의 생산성은 오히려 전체적으로 감소하는 경향을 보여 주었다. 이러한 결과는 소규모 배양방법을 확립하기 위해 새로이 개발한 포자형성배지와 성장배지의 조성변화로 인해 기존에 확립된 생산배지에서는 생산균주의 monacolin-K 생합성 능력이 최대로 발휘되지 않았기 때문에 발생한 것으로 판단되었다. 따라서 본 연구를 통해 확립된 소규모 성장배양에 적합한 최적의 생산배지 조성을 확립하고자 통계적 방법인 Plackett-Burman design을 적용하여 monacolin-K 생산성 향상에 영향을 주는 glycerol, malt extract, yeast extract, brown rice의 4가지 성분들을 최종 선별하였고, 또한 이들의 최적농도 결정을 위해 반응표면분석 실험을 수행하였다. 이와 같이 결정된 최적 생산배지를 사용하여 생산배양을 수행한 결과, monacolin-K의 생산성이 2배 이상 증가하고, 배양 형태 또한 용존산소와 영양분의 전달이 매우 용이한 직경 1mm 이하의 pellet 모양을 유지함을 확인할 수 있었다.

Effects of Processing Method on Performance and Nutrient Digestibility in Growing-finishing Pigs Fed Lupine Seeds

  • Yang, Y.X.;Kim, Y.G.;Heo, S.;Ohh, S.J.;Chae, B.J.
    • Asian-Australasian Journal of Animal Sciences
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    • 제20권8호
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    • pp.1229-1235
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    • 2007
  • Three trials were conducted to study the effect of processing method on performance, apparent nutrient and amino acid digestibilities in growing-finishing pigs fed lupine seeds. Ground, expanded or flaked lupine seed was prepared for the trials. In trial 1, a total of 72 growing barrows ($Landrace{\times}Large$ $White{\times}Large$, initial BW of $54.22{\pm}5.87$ kg) were allocated to three treatment diets containing 15% percent of differently processed lupine seed for 28 days. Each treatment had four replicates comprising 6 pigs each. In trial 2, a total of 48 finishing barrows ($Landrace{\times}Large$ $White{\times}Large$, initial BW of $84.40{\pm}6.84$ kg) were subjected to three treatments for 21 days. The experimental design was the same as trial 1 except for supplementation with 20% of lupine seed. Each treatment had four replicates comprising 4 pigs each. To evaluate the ileal digestibility of amino acids, a total of 9 barrows ($Landrace{\times}Large$ $White{\times}Large$), with an average initial BW of $41.07{\pm}1.98$ kg, were fed with ground, expanded or flaked lupine for 7 days. Each pig was surgically equipped with a simple T-cannula fitted in the distal ileum. Amino acid composition and presence of anti-nutritional factors (ANFs) in processed lupine sources were also evaluated. The results showed that there was no significant difference (p>0.05) in lupine amino acid composition due to the different processing methods. Expanded and flaked lupine significantly decreased (p<0.05) the concentration of ANFs compared with ground lupine. There was no effect (p>0.05) on the growth performance in growing pigs. However, processing method had a significant effect (p<0.05) on average daily gain (ADG), average daily feed intake (ADFI) and feed conversion ratio (FCR) in finishing pigs. There was no effect (p>0.05) of the different processing methods on the digestibility of dry matter (DM), gross energy (GE), ether extract (EE), Calcium (Ca) and phosphorus (P), but expanded lupine could significantly increase (p<0.05) the apparent digestibility of CP in finishing pigs. Furthermore, expanded lupine had a higher (p<0.05) apparent ileal digestibility for most indispensable and dispensable amino acids compared with ground and flaked lupine. It was concluded that expanded lupine could be beneficial in improving lupine's quality and improve performance and nutrient utilization in growing-finishing pigs.

한방기공체조가 두뇌력, 심박변이율, 생혈액형태에 미치는 영향 (Effect of the Oriental Medicine Gi-Gong Exercise on the Brain Power, HRV, Live Blood Condition in the Youth)

  • 이정원;김이순;김경철
    • 동의생리병리학회지
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    • 제21권1호
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    • pp.126-135
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    • 2007
  • This study was conducted to identify the effects of the oriental medicine Qigong Exercise on the brain power, HRV, pulsation, live blood condition among young boys and girls. The study was performed with two group(control group and experimental group) in a pre-test/post-test design. The subjects were 44 young boys and girls selected by a some middle school in Busan. The oriental medicine Qigong Exercise program consists of 80-minute sessions three times a week over 5 months. All of the subjects were examined on the congnition assessment tool, stress assessment tool, oriental medicine pulsation 3-D MAC, live blood condition analyzer Prior and post surveys were measured before and after the experiment. In the cognition assessment, the amplitude of ERS were increased afer Qigong Exercise. The Success and the Concentration were significantly increased afer Qigong Exercise, the Error was significantly decreased afer Qigong Exercise. The Cognition strength was significantly increased, but the Reponse time was not significantly decreased afer Qigong Exercise. And the Workload was not significantly decreased, the Total Score was not significantly increased afer Qigong Exercise. Among the stress assessment, RRV tachogram's ‘mean RR’ was significantly increased, ‘mean HRV’ was significantly decreased afer Qigong Exercise. SDNN was not significantly increased, Complexity was not significantly increased afer Qigong Exercise. And TP(RRV power's total power) was not significantly increased, VLF and HF was significantly increased, and LF was significantly decreased afer Qigong Exercise. ANS's norm LF was not significantly decreased, but norm HF was significantly increased afer Qigong Exercise. In the RRV's Phase Plot, RMSSD and SDSD were not significantly increased, pnn50 was not significantly decreased afer Qigong Exercise. On the whole, Parasympathetic Activity and Stress Endurance were significantly increased, but Cardiac Activity and Physical Arousal were not significantly increased afer Qigong Exercise. Cardiac Aging was not significantly decreased afer Qigong Exercise. Sympathetic Activity, Autonomic Nervous System Balance and Heart-load were not significantly decreased afer Qigong Exercise. In the pulsation, press power was increased(15%), and w/t(pressurization time / pulsation time) was decreased(20%) afer Oigong Exercise. And the live blood condition was not changed afer Qigong Exercise. As mentioned above; the oriental medicine Qigong Exercise program was identified the effects of the inspiration of the brain power, heart rate and anti-stress.

피부 미백제의 타겟 단백질인 인간 티로시나제의 3차원 구조 상동 모델링 (Comparative Modeling of Human Tyrosinase - an Important Target for Developing Skin Whitening Agents)

  • 최종근;서주원
    • 한국산학기술학회논문지
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    • 제13권11호
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    • pp.5350-5355
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    • 2012
  • 사람의 티로시나제는 사람의 피부색을 결정하는 멜라닌 생합성의 첫 번째 반응을 촉매하며, 이 단계는 반응 속도를 결정하는 가장 중요한 단계이다. 따라서, 많은 화장품 회사들은 hTyr의 저해제를 찾고자 하였으나 사람 티로시나제의 3차원구조의 부재로 구조기반의 가상탐색은 불가능하다. 따라서 본 연구에서는 구조기반의 저해제 탐색을 위하여 기존에 그 구조가 알려진 Bacillus megaterium의 티로시나제의 3차 구조를 이용하여 인간 티로시나제의 3차원 구조를 상동모델링법으로 예측하였다. 3차원 구조 분석 결과 인간 티로시나제의 활성부위에 위치한 여섯 개의 히스티딘 잔기가 2개의 구리원자와 결합할 수 있으며, 이 활성부위는 단백질의 안쪽에 위치함을 알 수 있었다. 기질 또는 저해제가 결합할 수 있는 결합부위는 단백질의 표면에서 안쪽 깊은 곳의 활성부위와 연결되어 있으며 입구 쪽은 넓고 납작했으며 활성부위로 갈수록 좁아지는 깔대기와 같은 모양의 구조를 하고 있었다. 자체 제작 소프트웨어를 활용하여 solvent accessible surface를 만들고 여기에 가장 최적의 위치 및 형태를 갖는 모델을 티로신과 저해제로 가장 잘 알려진 코직산의 결합모델을 제안하였다. 이 결과 티로신과 코직산의 페놀그룹의 히드록시 기능단의 산소가 정확히 구리와 배위결합하는 것을 알 수 있었다. 결론적으로 본 연구 결과는 새로운 미백제를 설계하고 스크리닝하는데 유용한 정보를 제공할 수 있을 것으로 사료된다.

Delphi/AHP를 활용한 다중이용업 신종업종의 화재위험평가지표 분석 (Analysis of Fire Risk Assessment Indicators of Publicly-Used Establishments using Delphi/AHP)

  • 김명철;김학중;박경환;윤해권;이승호
    • 한국화재소방학회논문지
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    • 제33권6호
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    • pp.87-94
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    • 2019
  • 국민권익위원회 2018년 7월 17일 보도 자료에 의하면 방탈출카페, 실내양궁장 등 신종업종이 다중이용업에서 제외되어 지정을 검토하고 화재예방 대책을 수립하도록 소방청에 권고하였다. 본 연구는 Delphi 기법으로 다중이용업 신종업종의 화재위험평가 측정영역 및 측정지표를 계층화하고 적합도(3.00 이상) 분석결과, 적합도 평균값이 4.25로서 적합성을 확보하였다. AHP 분석결과, 화재위험평가 측정영역의 일관성 비율은 4.0%로서 CR ≤ 0.1(10%) 보다 낮게 분석되었고 하위 측정지표의 일관성 비율도 0.1%~3.6%로서 모두 일관성이 있음을 확인하였다. 통합 측정지표의 중요도와 우선순위는 영업장 내부통로의 형태 및 출구의 피난능력(0.316), 점화원관리(0.141), 고유위험(0.106), 화재감지시스템의 적절성 및 적응성(0.097), 가연물관리(0.084), 피난안내 및 피난설비(0.075), 내화구조 및 마감재료(0.060), 구획 및 비상구(0.049), 연소확대위험(0.046), 소화설비의 적절성 및 적응성(0.026)의 순으로 도출 되었다. 본 연구는 향후, 화재위험평가지표 개발 연구의 중요한 자료로 널리 활용 될 것이다.

융복합 기반의 지역간 흡연율의 변이 분석 (Convergence-based analysis on geographical variations of the smoking rates)

  • 임지혜;강성홍
    • 디지털융복합연구
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    • 제13권8호
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    • pp.375-385
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    • 2015
  • 본 연구의 목적은 흡연율의 지역간 변이 정도와 흡연율에 영향을 미치는 요인을 살펴보는 데 있다. 분석을 위해 2009-2011년 질병관리본부의 지역사회건강조사 자료와 국민건강보험공단의 자료를 사용하였다. 흡연율에 영향을 미치는 요인을 파악하기 위해 상관분석, 다중회귀분석을 이용하였으며, 지역간 변이 분석을 위해 의사결정나무 모형을 이용하였다. 연구결과, 지역간 흡연율의 변이요인은 고위험음주율, 고혈압관리교육 이수율, 금연캠페인 경험율, 스트레스 인지율, 고혈압 유병률, 건강보험료, 당뇨병 유병률, 비만율, 근력운동 실천율로 나타났다. 융복합 기반으로 지역별 흡연율의 변이를 파악하는 것은 흡연율을 감소시키기 위한 지역별 맞춤형 보건프로그램을 수행하는데 매우 중요하다. 향후 흡연율이 높은 지역의 원인과 대상에 맞는 구체적인 관리방안을 모색하는 노력이 필요할 것이다. 본 연구의 결과는 효과적인 흡연관리사업 방안을 도출하는 데 필요한 기초자료로 의미있게 활용될 수 있으리라 여겨진다.

고품위화 정제공정 부산물(SDAR) 활용을 위한 첨가제 개발 및 이를 이용한 아스팔트 혼합물의 실내 공용성능 평가 (Development of Additive to Modify the SDAR (Solvent DeAsphalting Residue) and Laboratory Performance Evaluation of Asphalt Mixture with Modified SDAR)

  • 백철민;양성린;황성도
    • 한국도로학회논문집
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    • 제18권6호
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    • pp.97-104
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    • 2016
  • OBJECTIVES : The objective of this research is to develop additives for the modification of Solvent DeAsphalting Residue (SDAR) to be used as pavement materials, and evaluate the performance of asphalt mixture manufactured using the SDAR modified by developed additives. METHODS : The SDAR generally consists of more asphaltenes and less oil components compared to the conventional asphalt binder, and hence, the chemical/physical properties of SDAR are different from that of conventional asphalt binder. In this research, the additives are developed using the low molecular oil-based plasticizer to improve the properties of SDAR. First, the chemical property of two SDARs is analyzed using SARA (saturate, aromatic, resin, and asphaltene) method. The physical/rheological properties of SDARs and SDARs containing additives are also evaluated based on PG-grade method and dynamic shear-modulus master curve. Second, various laboratory tests are conducted for the asphalt mixture manufactured using the SDAR modified with additives. The laboratory tests conducted in this study include the mix design, compactibility analysis, indirect tensile test for moisture susceptibility, dynamic modulus test for rheological property, wheel-tracking test for rutting performance, and direct tension fatigue test for cracking performance. RESULTS : The PG-grade of SDARs is higher than PG 76 in high temperature grades and immeasurable in low temperature grades. The dynamic shear modulus of SDARs is much higher than that of conventional asphalt, but the modified SDARs with additives show similar modulus compared to that of conventional asphalt. The moisture susceptibility of asphalt mixture with modified SDARs is good if, the anti-stripping agent is included. The performance (dynamic modulus, rutting resistance, and fatigue resistance) of asphalt mixture with modified SDARs is comparable to that of conventional asphalt mixture when appropriate amount of additives is added. CONCLUSIONS : The saturate component of SDARs is much less than that of conventional asphalt, and hence, it is too hard and brittle to be used as pavement materials. However, the modified SDARs with developed additives show comparable or better rheological/physical properties compared to that of conventional asphalt depending on the type of SDAR and the amount of additives used.

6종의 미세조류 추출물의 탈모예방 및 두피 개선 효과 비교 (Comparative Study on the Effects of Six Species of Microalgae Extracts on Hair Loss Prevention and Scalp Improvement)

  • 정민화;이수선;김현아;황혜정
    • 생명과학회지
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    • 제28권3호
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    • pp.345-350
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    • 2018
  • 이번 연구에서 우리는 6종의 미세조류 ; Phaeodacylum tricrnutum (PT), Chaetoceros gracilis (CG), Nanochloris oculata (NO), Pavlova lutheri (PL), Chlorella ellipsoidae (CE), and Scendedemus obliquus (SO)를 이용하여 탈모 예방 및 두피 개선효과를 살펴보았다. DPPH 라디칼 소거능 실험에서는 SO, CG, ST9 순으로 활성이 높은 것으로 나타났다. OH 소거능 실험 결과, CG (6.6~42.1%), ST9 (26.0~44.0%), SO (7.8~44.3%)으로 유의적인 효과를 보였다. 그외에도 세포증식실험에서는, SO가 인간표피세포주인 HaCaT 세포 증식을 촉진시켰으며, CG와 SO가 모유두세포주인 HFDPC 세포 증식을 촉진시키는 것으로 나타났다. 마지막으로 5-alpha reductase 저해능 실험결과, PT, CG, SO가 유의적으로 효소활성을 저해하였다. 따라서, 이번 연구에 사용한 6종의 미세조류들 중, 항산화활성, 세포증식 효과, 5-alpha reductase 저해능을 보인 몇가지 조류들이 탈모예방 및 두피개선을 위한 제품개발에 좋은 원료가 될 가능성이 있음을 제시한다.

Effects of dietary enzyme cocktail on growth performance, intestinal morphology, and nutrient digestibility of weaned pigs

  • Kim, Yunkang;Baek, Jangryeol;Jang, Kibeom;Kim, Junsu;Kim, Sheena;Mun, Daye;Kim, Byeonghyeon;Kim, Younghwa;Park, Juncheol;Choe, Jeehwan;Song, Minho
    • 농업과학연구
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    • 제44권4호
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    • pp.513-518
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    • 2017
  • Soybean, one of most widely used swine feed component in the world, contains non-starch polysaccharides (NSP). The digestive system of weaned pigs is not yet fully developed, and thus weaned pigs cannot easily digest diets based on corn and soybean meal. Dietary exogenous enzymes supplementation has been intensively investigated to assist digestion of anti-nutritional factors, such as NSP. This experiment was conducted to investigate the effects of dietary enzyme cocktail on growth performance, intestinal morphology, and nutrient digestibility of weaned pigs. A total 36 weaned pigs ($5.92{\pm}0.48kg\;BW$; 28 d old) were randomly allotted to 2 dietary treatments (3 pigs/pen, 6 replicates/treatment) in a randomized complete block design. The dietary treatments were a typical diet based on corn and soybean meal (CON) and CON with 0.05% enzyme cocktail (Cocktail; mixture of xylanase, ${\alpha}-amylase$, protease, ${\beta}-glucanase$, and pectinase). Pigs were fed their respective diets for 6 wk. Growth performance, morphology of ileum, apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of dry matter, crude protein, and energy of weaned pigs were measured. No significant differences (p > 0.05) were observed for growth performance for the duration of the experimental period, and morphology of ileum, and nutrient digestibility between CON and Cocktail treatment groups. Therefore, the results from the current study indicated that enzyme cocktail supplementation in diets had no influence on growth performance, intestinal morphology, and nutrient digestibility of weaned pigs.

인류학적(人類學的) 분류(分類)에 따른 스트리트 스타일의 발생(發生)과 계보(系譜)에 관한 연구(硏究) (A Study of Origination and Genealogy on Street Style according to Anthropology)

  • 이영재
    • 패션비즈니스
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    • 제11권4호
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    • pp.183-203
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    • 2007
  • This study aims at providing useful fundamental information to re-establish the theories of modern fashion by examining the origination and genealogy of street style. The street styles focusing on caucasoid have a variety of genealogies such as western type, beat, teddy boy, hippie, skinhead, punk, neuron-mantic, indie kid, riot grrrl, grunge and techno cyber punk. In the same period, on the contrary, the streets styles focusing on negroid are zootie, hipster, modernist, rude boy, two-tone, rastafarian, funky, B-boy, fly girl, raggamuffine, bhangra, and acid jazz, which are seen as the culture of the large cities formed along Atlantic Ocean and Caribbean sea like England, America and Jamaica. These have root as the main fashion in western society. Ironically, most of the subculture concentrated on the whites were racists. Because of such a reason, the street styles have been formed as resistance culture that was unable to sympathize with their society and characteristics by distinguishing the whites and the colored people. Zootie or hipster that is one of the street fashion styles was formed in the 1940-50s, while the colored people who lived in the west Indies migrated to England or America. As a minimal modernist style called Ivy look in US, in that time, anti-culture formed by teenagers in whitey, teddy boy and mods fashion can be strictly different from the zootie and hipster. The colored people's street styles of the 1960s developed into aggressive and hard forms from the rude boy and two-tone while their resistance toward the whites was stronger. The rastafarian style researched the peak as the colored people's traditional ethnic characteristics or resistance intention for their freedom in the 1970s. In that time, The colored people's street styles of the 1960s developed into aggressive and hard forms from the rude boy and two-tone while their resistance toward the whites was stronger. The rastafarian style researched the peak as the colored people's traditional ethnic characteristics or resistance intention for their freedom in the 1970s. In that time, the street styles of the whites were mostly the skinhead or hippie. Most of them were racists toward the colored people. The punk type on shown on the whites focused on luxury and exaggerative costume. On the contrary, the funky style of the colored people focused on aggressive nihilism and form. With B-boy, fly girl, reggae, rap music, and break dancing in the 1980s, the subculture gradually told on the high fashion as well as the culture between the whites and the colored people. From such aspects, the colored people tried to maintain their unique traditional characteristics. However, their individual values surged by the coming young generation excluded the colored people's characteristic street styles. Focusing on gender, violence and private success among their major concerns, the raga muffin style that represents multi-races and multi-cultures was formed. The jazz style in the 1990s showed cold post-modernistic eclecticism different from that of the 1940s-50s. Simultaneously, the various classes appeared their street styles by emphasizing on each personality. Now that we are living in multi-cultural society, a human race or nationalism concept is getting obscurer. There is no obvious boundary line in the differences between human race and its fashion.