• Plant Breeding and Biotechnology
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• v.5 no.3
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• pp.243-251
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• 2017

Rhus chinensis is a shrub widely distributed in Asia. It has been used for traditional medicine and ecological restoration. Here, we report the complete chloroplast genome sequence of two R. chinensis genotypes collected from China and Korea. The assembled chloroplast genome of Chinese R. chinensis is 149,094 bp long, consisting of a large single copy (97,246 bp), a small single copy (18,644 bp) and a pair of inverted repeats (16,602 bp). Gene annotation revealed 77 protein coding genes, 30 tRNA genes, and 4 rRNA genes. A phylogenomic analysis of the chloroplast genomes with 11 known complete chloroplast genomes clarified the relationship of R. chinensis with the other plant species in the Sapindales order. A comparative chloroplast genome analysis identified 170 SNPs and 85 InDels at intra-species level of R. chinensis between Chinese and Korean collections. Based on the sequence diversity between Korea and Chinese R. chinensis plants, we developed three DNA markers useful for genetic diversity and authentication system. The chloroplast genome information obtained in this study will contribute to enriching genetic resources and conservation of endemic Rhus species.

• Journal of the Institute of Convergence Signal Processing
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• v.22 no.4
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• pp.149-155
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• 2021

In this study, we intend to develop a defective road surface object recognition model that automatically detects road surface defects that restrict the movement of the transportation handicapped using electric mobile devices with deep learning. For this purpose, road surface information was collected from the pedestrian and running routes where the electric mobility aid device is expected to move in five areas within the city of Busan. For data, images were collected by dividing the road surface and surroundings into objects constituting the surroundings. A series of recognition items such as the detection of breakage levels of sidewalk blocks were defined by classifying according to the degree of impeding the movement of the transportation handicapped in traffic from the collected data. A road surface object recognition deep learning model was implemented. In the final stage of the study, the performance verification process of a deep learning model that automatically detects defective road surface objects through model learning and validation after processing, refining, and annotation of image data separated and collected in units of objects through actual driving. proceeded.

• Animal Bioscience
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• v.35 no.11
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• pp.1808-1816
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• 2022

Objective: The study of Hanwoo (Korean native cattle) has mainly been focused on meat quality and productivity. Recently the field of microbiome research has increased dramatically. However, the information on the microbiome in Hanwoo is still insufficient, especially relationship between vagina and feces. Therefore, the purpose of this study is to examine the microbial community characteristics by analyzing the 16S rRNA sequencing data of Hanwoo vagina and feces, as well as to confirm the difference and correlation between vaginal and fecal microorganisms. As a result, the goal is to investigate if fecal microbiome can be used to predict vaginal microbiome. Methods: A total of 31 clinically healthy Hanwoo that delivered healthy calves more than once in Cheongju, South Korea were enrolled in this study. During the breeding season, we collected vaginal and fecal samples and sequenced the microbial 16S rRNA genes V3-V4 hypervariable regions from microbial DNA of samples. Results: The results revealed that the phylum-level microorganisms with the largest relative distribution were Firmicutes, Actinobacteria, Bacteroidetes, and Proteobacteria in the vagina, and Firmicutes, Bacteroidetes, and Spirochaetes in the feces, respectively. In the analysis of alpha, beta diversity, and effect size measurements (LefSe), the results showed significant differences between the vaginal and fecal samples. We also identified the function of these differentially abundant microorganisms by functional annotation analyses. But there is no significant correlation between vaginal and fecal microbiome. Conclusion: There is a significant difference between vaginal and fecal microbiome, but no significant correlation. Therefore, it is difficult to interrelate vaginal microbiome as fecal microbiome in Hanwoo. In a further study, it will be necessary to identify the genetic relationship of the entire microorganism between vagina and feces through the whole metagenome sequencing analysis and meta-transcriptome analysis to figure out their relationship.

• KIPS Transactions on Software and Data Engineering
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• v.12 no.4
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• pp.159-172
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• 2023

In general, social problem-solving research aims to create important social value by offering meaningful answers to various social pending issues using scientific technologies. Not surprisingly, however, although numerous and extensive research attempts have been made to alleviate the social problems and issues in nation-wide, we still have many important social challenges and works to be done. In order to facilitate the entire process of the social problem-solving research and maximize its efficacy, it is vital to clearly identify and grasp the important and pressing problems to be focused upon. It is understandable for the problem discovery step to be drastically improved if current social issues can be automatically identified from existing R&D resources such as technical reports and articles. This paper introduces a comprehensive dataset which is essential to build a machine learning model for automatically detecting the social problems and solutions in various national research reports. Initially, we collected a total of 700 research reports regarding social problems and issues. Through intensive annotation process, we built totally 24,022 sentences each of which possesses its own category or label closely related to social problem-solving such as problems, purposes, solutions, effects and so on. Furthermore, we implemented four sentence classification models based on various neural language models and conducted a series of performance experiments using our dataset. As a result of the experiment, the model fine-tuned to the KLUE-BERT pre-trained language model showed the best performance with an accuracy of 75.853% and an F1 score of 63.503%.

• Korean Journal of Remote Sensing
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• v.39 no.6_3
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• pp.1679-1692
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• 2023

South Korea is a country that emits a large amount of pollutants as a result of population growth and industrial development and is also severely affected by transboundary air pollution due to its geographical location. As pollutants from both domestic and foreign sources contribute to air pollution in Korea, the location of air pollutant emission sources is crucial for understanding the movement and distribution of pollutants in the atmosphere and establishing national-level air pollution management and response strategies. Based on this background, this study aims to effectively acquire spatial information on domestic and international air pollutant emission sources, which is essential for analyzing air pollution status, by utilizing high-resolution optical satellite images and deep learning-based image segmentation models. In particular, industrial parks and quarries, which have been evaluated as contributing significantly to transboundary air pollution, were selected as the main research subjects, and images of these areas from multi-purpose satellites 3 and 3A were collected, preprocessed, and converted into input and label data for model training. As a result of training the U-Net model using this data, the overall accuracy of 0.8484 and mean Intersection over Union (mIoU) of 0.6490 were achieved, and the predicted maps showed significant results in extracting object boundaries more accurately than the label data created by course annotations.

• The Journal of the Korea institute of electronic communication sciences
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• v.18 no.6
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• pp.1321-1330
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• 2023

This study attempts to address the problem of 3D pose estimation for multiple human objects through a single image generated during the character development process that can be used in augmented reality. In the existing top-down method, all objects in the image are first detected, and then each is reconstructed independently. The problem is that inconsistent results may occur due to overlap or depth order mismatch between the reconstructed objects. The goal of this study is to solve these problems and develop a single network that provides consistent 3D reconstruction of all humans in a scene. Integrating a human body model based on the SMPL parametric system into a top-down framework became an important choice. Through this, two types of collision loss based on distance field and loss that considers depth order were introduced. The first loss prevents overlap between reconstructed people, and the second loss adjusts the depth ordering of people to render occlusion inference and annotated instance segmentation consistently. This method allows depth information to be provided to the network without explicit 3D annotation of the image. Experimental results show that this study's methodology performs better than existing methods on standard 3D pose benchmarks, and the proposed losses enable more consistent reconstruction from natural images.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.22 no.6
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• pp.114-123
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• 2023

With the development of computer vision technology, video sensors such as CCTV are detecting incident. However, most of the current incident have been detected based on existing fixed imaging equipment. Accordingly, there has been a limit to the detection of incident in shaded areas where the image range of fixed equipment is not reached. With the recent development of edge-computing technology, real-time analysis of mobile image information has become possible. The purpose of this study is to evaluate the possibility of detecting expressway emergencies by introducing computer vision technology to dash cam. To this end, annotation data was constructed based on 4,388 dash cam still frame data collected by the Korea Expressway Corporation and analyzed using the YOLO algorithm. As a result of the analysis, the prediction accuracy of all objects was over 70%, and the precision of traffic accidents was about 85%. In addition, in the case of mAP(mean Average Precision), it was 0.769, and when looking at AP(Average Precision) for each object, traffic accidents were the highest at 0.904, and debris were the lowest at 0.629.

• Molecules and Cells
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• v.47 no.3
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• pp.100033.1-100033.13
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• 2024

Considering the recent increase in the number of colorectal cancer (CRC) cases in South Korea, we aimed to clarify the molecular characteristics of CRC unique to the Korean population. To gain insights into the complexities of CRC and promote the exchange of critical data, RNA-sequencing analysis was performed to reveal the molecular mechanisms that drive the development and progression of CRC; this analysis is critical for developing effective treatment strategies. We performed RNA-sequencing analysis of CRC and adjacent normal tissue samples from 214 Korean participants (comprising a total of 381 including 169 normal and 212 tumor samples) to investigate differential gene expression between the groups. We identified 19,575 genes expressed in CRC and normal tissues, with 3,830 differentially expressed genes (DEGs) between the groups. Functional annotation analysis revealed that the upregulated DEGs were significantly enriched in pathways related to the cell cycle, DNA replication, and IL-17, whereas the downregulated DEGs were enriched in metabolic pathways. We also analyzed the relationship between clinical information and subtypes using the Consensus Molecular Subtype (CMS) classification. Furthermore, we compared groups clustered within our dataset to CMS groups and performed additional analysis of the methylation data between DEGs and CMS groups to provide comprehensive biological insights from various perspectives. Our study provides valuable insights into the molecular mechanisms underlying CRC in Korean patients and serves as a platform for identifying potential target genes for this disease. The raw data and processed results have been deposited in a public repository for further analysis and exploration.

• Journal of Life Science
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• v.34 no.3
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• pp.179-190
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• 2024

Cannabis sativa is a plant widely cultivated worldwide and has been used as a material for food, medicine, building materials and cosmetics. In this study, we assessed the functional effects of C. sativa stem and root extracts using network pharmacology and confirmed their novel functions. The components in stem and root ethanol extracts were identified by gas chromatography-mass spectrometry analysis, and networks between the components and proteins were constructed using the STICHI database. Functional annotation of the proteins was performed using the KEGG pathway. The effects of the extracts were confirmed in lysophosphatidylcholine-induced THP-1 cells using real-time PCR. A total of 21 and 32 components were identified in stem and root extracts, respectively, and 147 and 184 proteins were linked to stem and root components, respectively. KEGG pathway analysis showed that 69 pathways, including the MAPK signaling pathway, were commonly affected by the extracts. Further investigation using pathway networks revealed that terpenoid backbone biosynthesis was likely affected by the extracts, and the expression of the MVK and MVD genes, key proteins in terpenoid backbone biosynthesis, was decreased in LPC-induced THP-1 cells. Therefore, this study determined the diverse function of C. sativa extracts, providing information for predicting and researching the effects of C. sativa.

• Journal of Plant Biotechnology
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• v.33 no.3
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• pp.153-160
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• 2006

Brassica rape is an important species used as a vegetable, oil, and fodder worldwide. It is related phylogenically to Arabidopsis thaliana, which has already been fully sequenced as a model plant. The 'Multinational Brassica Genome Project (MBGP)'was launched by the international Brassica community with the aim of sequencing the whole genome of B. rapa in 2003 on account of its value and the fact that it has the smallest genome among the diploid Brassica. The genome study was carried out not only to know the structure of genome but also to understand the function and the evolution of the genes comprehensively. There are two mapping populations, over 1,000 molecular markers and a genetic map, 2 BAC libraries, physical map, a 22 cDHA libraries as suitable genomic materials for examining the genome of B. rapa ssp. pekinensis Chinese cabbage. As the first step for whole genome analysis, 220,000 BAC-end sequences of the KBrH and KBrB BAC library are achieved by cooperation of six countries. The results of BAC-end sequence analysis will provide a clue in understanding the structure of the genome of Brassica rapa by analyzing the gene sequence, annotation and abundant repetitive DHA. The second stage involves sequencing of the genetically mapped seed BACs and identifying the overlapping BACs for complete genome sequencing. Currently, the second stage is comprises of process genetic anchoring using communal populations and maps to identify more than 1,000 seed BACs based on a BAC-to-BAC strategy. For the initial sequencing, 629 seed BACs corresponding to the minimum tiling path onto Arabidopsis genome were selected and fully sequenced. These BACs are now anchoring to the genetic map using the development of SSR markers. This information will be useful for identifying near BAC clones with the seed BAC on a genome map. From the BAC sequences, it is revealed that the Brassica rapa genome has extensive triplication of the DNA segment coupled with variable gene losses and rearrangements within the segments. This article introduces the current status and prospective of Korea Brassica Genome Project and the bioinformatics tools possessed in each national team. In the near future, data of the genome will contribute to improving Brassicas for their economic use as well as in understanding the evolutional process.