• Asian-Australasian Journal of Animal Sciences
• /
• 제17권7호
• /
• pp.960-966
• /
• 2004

The purpose of this study was to evaluate the effects of protein supplementation of green tea waste (GTW) on the performance of lactating cows. Another aim was to increase resource utilization and to eliminate any environmental negative impact from the tea waste. GTW from a beverage company was ensiled at a low pH (<4.0) and high acetic acid and lactic acid concentration, and it contained high crude protein (CP, 34.8%), total extractable tannins (TET, 9.2%) and condensed tannin (CT, 1.7%). Two experiments were conducted to investigate the palatability and performance in lactating cows fed GTW. In the palatability trial, three lactating cows were allocated to three dietary treatments in a 3$\times$3 Latin square design. The animals were offered a total mixed ration (TMR) including GTW at rates of 0, 2.5 and 5.0% on a dry matter (DM) basis. Total DM intake was not different among the treatments. In the performance trial, four lactating cows were used in a 2$\times$2 Latin square design with a 3 week sampling period. GTW was incorporated into TMR at a rate of 5.0% on a DM and 10.0% on a CP basis. Thus GTW replaced alfalfa hay and soybean meal at a level of 25.0% on a DM. DM and CP intake were not affected by the inclusion of GTW, whereas TET and CT intake were significantly increased (p<0.001). Milk production, milk composition and the efficiency of milk production were not altered by the GTW inclusion. Although ruminal pH and VFA, and blood urea nitrogen were not changed, ruminal $NH_{3}-N$ and plasma total cholesterol were relatively low in the GTW group, but not significantly different. The excretion of urinary purine derivatives and estimated MN supply were also not significantly affected by GTW treatment. It is therefore concluded that GTW can be used as a protein source without any detrimental effects on the performance of lactating cows.

• Asian-Australasian Journal of Animal Sciences
• /
• 제25권6호
• /
• pp.869-879
• /
• 2012

This study was conducted to investigate the effects of dietary lipid source and level on growth performance, blood parameters, fatty acid composition and flesh quality of sub-adult olive flounder Paralichthys olivaceus. Eight experimental diets were formulated to contain 5% squid liver oil (SLO), 5% linseed oil (LO), 5% soybean oil (SO), a mixture of 1% squid liver oil, 2% linseed oil and 2% soybean oil (MIX), no lipid supplementation with high protein level (LL-HP), 10% squid liver oil (HL-SLO), a mixture of 1% squid liver oil, 4.5% linseed oil and 4.5% soybean oil (HL-VO), and 1% squid liver oil with high starch level (LL-HC), respectively. Two replicate groups of fish (average initial weight of 296 g) were fed the diets for 17 wks. After 5 wks, 11 wks and the end of the feeding trial, five fish from each tank were randomly sampled for analysis of body composition. At the end of the feeding trial, final mean weight of fish fed the LL-HP diet was significantly (p<0.05) higher than that of fish fed the HL-VO diet, but did not differ significantly from those of fish fed the SLO, LO, SO, MIX, HL-SLO and LL-HC diets. Fish fed the LL-HP diet showed significantly higher feed efficiency than fish fed the LO, HL-SLO and HL-VO diets. Feed efficiency of fish fed the LO, SO and MIX diets were similar to those of fish fed the SLO and HL-SLO diets. Fish fed the HL-SLO diet showed significantly higher total cholesterol content in plasma compared with other diets. Fatty acid composition of tissues was reflected by dietary fatty acid composition. The highest linoleic (LA) and linolenic acid (LNA) contents in the dorsal muscle were observed in fish fed the SO and LO diets, respectively, regardless of feeding period. The highest eicosapentaenoic acid (EPA) content in the dorsal muscle was observed in fish fed the LL-HP and LL-HC diets after 11 and 17 weeks of feeding, respectively. Fish fed the SLO and HL-SLO diets showed higher docosahexaenoic acid (DHA) content than that of other treatments after 11 and 17 weeks of feeding, respectively. Dietary inclusion of vegetable oils reduced n-3 HUFA contents in the dorsal muscle and liver of fish. The n-3 HUFA contents in tissues of fish fed the SLO and HL-SLO diets were higher than those of fish fed other diets, except for the LL-HP and LL-HC diets. Hardness, gel strength, chewiness and cohesiveness values of dorsal muscle in fish were significantly affected by dietary lipid source. The results of this study indicate that fish oil in fish meal based diets for sub-adult olive flounder could be replaced by soybean oil and linseed oil without negative effects on growth and feed utilization.

• Asian-Australasian Journal of Animal Sciences
• /
• 제30권11호
• /
• pp.1550-1556
• /
• 2017

Objective: This study estimated the genetic parameters for productive and reproductive traits. Methods: The data included production and reproduction records of animals that have calved between 1979 and 2013. The genetic parameters were estimated using multivariate mixed models (DMU) package, fitting univariate and multivariate mixed models with average information restricted maximum likelihood algorithm. Results: The estimates of heritability for milk production traits from the first three lactation records were $0.03{\pm}0.03$ for lactation length (LL), $0.17{\pm}0.04$ for lactation milk yield (LMY), and $0.15{\pm}0.04$ for 305 days milk yield (305-d MY). For reproductive traits the heritability estimates were, $0.09{\pm}0.03$ for days open (DO), $0.11{\pm}0.04$ for calving interval (CI), and $0.47{\pm}0.06$ for age at first calving (AFC). The repeatability estimates for production traits were $0.12{\pm}0.02$, for LL, $0.39{\pm}0.02$ for LMY, and $0.25{\pm}0.02$ for 305-d MY. For reproductive traits the estimates of repeatability were $0.19{\pm}0.02$ for DO, and to $0.23{\pm}0.02$ for CI. The phenotypic correlations between production and reproduction traits ranged from $0.08{\pm}0.04$ for LL and AFC to $0.42{\pm}0.02$ for LL and DO. The genetic correlation among production traits were generally high (>0.7) and between reproductive traits the estimates ranged from $0.06{\pm}0.13$ for AFC and DO to $0.99{\pm}0.01$ between CI and DO. Genetic correlations of productive traits with reproductive traits were ranged from -0.02 to 0.99. Conclusion: The high heritability estimates observed for AFC indicated that reasonable genetic improvement for this trait might be possible through selection. The $h^2$ and r estimates for reproductive traits were slightly different from single versus multi-trait analyses of reproductive traits with production traits. As single-trait method is biased due to selection on milk yield, a multi-trait evaluation of fertility with milk yield is recommended.

• 한국유기농업학회지
• /
• 제20권2호
• /
• pp.185-200
• /
• 2012

지구상의 인구는 계속해서 증가하고 식량의 안정적인 수급은 한 국가의 문제가 아니라 국가와 지역을 뛰어넘는 전 세계적인 문제이다. 경제의 발전과 더불어 개발도상국가들의 육류 및 유제품의 섭취가 급증하고 있고 선진국들의 축산물 섭취량 또한 섭취형태에 차이가 있을 뿐 증가하고 있는 추세이다. 축산물 생산에 따르는 환경파괴, 특히 반추동물산업에 서의 온실가스 발생량은 심각한 것으로 보고되었는데 이러한 온실가스를 줄이기 위한 노력에 전 세계가 동참하고 있다. 이러한 노력의 일환으로 많은 연구가 진행되고 있는데 생산성 및 효율의 증대, 가축 개량 등의 노력이 진행되어 왔고 사양적 측면에서 사료배합기술의 발달, 화학적 첨가제 등을 사용했었다. 이들뿐만 아니라 최근에는 생균제, 식물추출물, 방목지에 적합한 새로운 방목 품종의 개발 등에 연구의 노력이 더해지고 있다. 반추동물산업은 인간이 섭취하지 못하는 식물세포벽 성분이나 비단백태 질소화합물을 이용하여 영양 가치가 우수한 고기와 우유를 생산하므로 다가올 식량안보에 크게 기여할 수 있다. 따라서 반추동물산업은 환경을 저해하는 요소를 줄이고자 지속적인 연구와 노력을 투자한다면 인간에게 고급 단백질을 공급하는 산업으로 오랜 기간 자리매김을 할 수 있을 것이다.

• 한국가금학회지
• /
• 제42권4호
• /
• pp.315-325
• /
• 2015

최근 소비자의 소득 수준이 향상되고, 육제품의 다원화 성향이 증가하면서 가축개량은 과거 성장 및 육량 중심의 개량에서 품질 중심의 개량으로 중심이 이동하고 있다. 특히, 식육의 품질 중에서 육색은 소비자가 식육을 선택 및 질을 판단하는 기준으로 작용하기 때문에 매우 중요한 형질이라고 볼 수 있다. 경제적으로 유용한 형질은 대부분 측정가능한 연속변이에 해당하고, 이러한 형질은 대부분 여러 유전자가 형질에 영향을 미치는 양적형질 좌위(Quantitative Trait Loci;QTL)에 속한다. Chicken QTL db에 보고된 닭의 육색형질과 관련된 형질들은 육색(Bco, Mco), 가열감량(DL), pH가 보고되어 있으며, 이는 닭의 13개 염색체에서 33개 QTL 및 association 영역이 보고되고 있다. 이 중에서 육색관련 후보 유전자는 APP, BCMO1, COL1A2, FTO, KPNA2, PSMD12, G0S2, FTSJ3가 있으며, 가열감량관련 후보유전자는 AGRP, FTO, pH와 관련된 후보유전자는 GALNT1, PCDH19, DIAPH1, SPP2 유전자로 총 14개 유전자가 확인되었다. 이렇게 확인된 후보유전자 및 QTL 연구결과는 한국재래닭에 적용 및 활용 가능성을 확인해 볼 필요가 있으며, 이러한 적용은 낮은 성장속도의 단점을 가진 한국재래닭의 개발에 있어 품질의 개량속도를 높여 산업적 가치를 빠르게 끌어올릴 수 있는 중요한 표지인자가 될 수 있을 것으로 사료된다.

• 대한환경공학회지
• /
• 제31권1호
• /
• pp.35-41
• /
• 2009

본 연구는 토착미생물에 의한 발효기작을 이용하여 음식물쓰레기를 동물 사료로 이용하기 위한 가능성을 평가하기 위하여 수행되었다. 이를 위하여 효소활성 실험을 통하여 anylase, protease, lipase에 대한 우수활성을 나타내는 토착유효미생물을 토양으로부터 분리하여 음식물쓰레기의 사료화를 위한 발효제로 사용하였다. 16s rDNA sequencing의 분석을 통해 우수활성을 나타내는 미생물(H1, D1)은 Bacillus subtilis와 Paenibacillus polymyxa인 것으로 확인되었다. 사료화 실험에서는 수분과 영양소 함량의 조절을 위하여 쌀겨 및 팽화미를 음식물쓰레기에 혼합하여 사료화를 위한 시료로 사용하였으며 효소활성 실험을 통해 분리된 2종의 미생물(Bacillus subtilis, Paenibacillus polymyxa)을 발효제로 사용하여 사료화 실험을 진행하였다. 발효실험 동안의 온도, pH 및 ORP 측정을 통해 토착유효미생물의 접종에 의한 음식물쓰레기의 발효가 효과적으로 진행됨을 확인할 수 있었다. 반응종료후 제조된 사료의 영양분 분석 결과에서 조단백질, 조지방, 조섬유은 동물 급여에 충분한 함량을 나타내었다. 또한 Pb, Hg, Cd, aflatoxin 및 salmonella 등의 유해물질의 분석결과 모든 항목이 허용기준치 이하의 값을 나타내었다. 따라서 토착유효미생물을 이용한 음식물쓰레기의 사료화는 동물의 사료로써 충분히 활용될 수 있을 것으로 기대된다.

• 한국가금학회지
• /
• 제32권4호
• /
• pp.275-279
• /
• 2005

본 시험은 시중에 유통되고 있는 일반 건조 잔반을 AO 배양물로서 산란계에 급여하여, 산란계의 생산성과 계란 품질에 미치는 영향을 구명하고자 실시하였다. 시험축은 평균 체중이 $1.5\pm0.5kg$인 50주령 산란계(ISA-Brown)를 공시하였고, 기초사료로 산란계 중기 사료를 급여하였으며, 시험사료는 대조구(Control), 잔반사료(FW)와 AO 배양물사료(AFW)를 각각 $25\%,\;50\%,\;75\%$씩 기초사료를 대체하여 총 7처리구, 3반복, 반복당 8수씩 168수를 공시하였다. 시험기간 중 사료섭취량에서 전처리구간 차이는 없었으나, FW구의 $50\%$ 처리구에서 대조구에 비해 유의적으로 감소했고, 산란률에 있어서는 대조구 $89.8\%$에 비해 AFW $25\%$ 처리구에서는 차이가 없었으나, 전 처리구에서 유의적으로 낮았다(p<0.05). 음수량은 잔반 사료의 대체 수준에 따라 증가하였다(p<0.05). 난중, 난각 두께 및 난각 강도는 전 처리구간 차이가 없었다. 호우유니트와 난황색에서는 남은 음식물 사료의 첨가에 따라 뚜렷한 차이를 보였으나(p<0.05) $50\%$ 이상의 첨가에서는 차이가 없었다. 남은 음식물사료의 첨가에 따른 난황내 콜레스테롤 함량에는 전 처리구간 차이가 없었다. 이러한 결과로부터, 잔반 사료는 산란율을 떨어뜨리지만, 난질 개선에 효과가 있고, 산란계 사료의 첨가제로서 AO 접종 잔반 사료의 사용이 가능할 수 있음을 시사한다.

• Animal Bioscience
• /
• 제34권4호
• /
• pp.525-532
• /
• 2021

Objective: Old World camels are a valuable genetic resource for many countries around the world due to their adaptation to the desert environment. At present, Old World camels have encountered the challenge of unprecedented loss of genetic resources. Through our research, we would reveal the population structure and genetic variation in Old World camel populations, which provides a theoretical basis for understanding the germplasm resources and origin and evolution of different Old World camel populations. Methods: In the present study, we assessed mtDNA control region sequences of 182 individuals from Old World camels to unravel genetic diversity, phylogeography, and demographic dynamics. Results: Thirty-two haplotypes confirmed by 54 polymorphic sites were identified in the 156 sequences, which included 129 domestic and 27 wild Bactrian camels. Meanwhile, 14 haplotypes were defined by 47 polymorphic sites from 26 sequences in the dromedaries. The wild Bactrian camel population showed the lowest haplotype and nucleotide diversity, while the dromedaries investigated had the highest. The phylogenetic analysis suggests that there are several shared haplotypes in different Bactrian camel populations, and that there has been genetic introgression between domestic Bactrian camels and dromedaries. In addition, positive values of Tajima's D and Fu's Fs test demonstrated a decrease in population size and/or balancing selection in the wild Bactrian camel population. In contrast, the negative values of Tajima's D and Fu's Fs test in East Asian Bactrian camel populations explained the demographic expansion and/or positive selection. Conclusion: In summary, we report novel information regarding the genetic diversity, population structure and demographic dynamics of Old World camels. The findings obtained from the present study reveal that abundant genetic diversity occurs in domestic Bactrian camel populations and dromedaries, while there are low levels of haplotype and nucleotide diversity in the wild Bactrian camel population.

• 한국동물생명공학회지
• /
• 제37권4호
• /
• pp.292-297
• /
• 2022

Direct injection of CRISPR/Cas9 into zygotes enables the production of genetically modified nonhuman primates (NHPs) essential for modeling specific human diseases, such as Usher syndrome, and for developing novel therapeutic strategies. Usher syndrome is a rare genetic disease that causes loss of hearing, retinal degeneration, and problems with balance, and is attributed to a mutation in MYO7A, a gene that encodes an uncommon myosin motor protein expressed in the inner ear and retinal photoreceptors. To produce an Usher syndrome type 1B (USH1B) rhesus macaque model, we disrupted the MYO7A gene in developing zygotes. Identification of appropriately edited MYO7A embryos for knockout embryo transfer requires sequence analysis of material recovered from a trophectoderm (TE) cell biopsy. However, the TE biopsy procedure is labor intensive and could adversely impact embryo development. Recent studies have reported using cell-free DNA (cfDNA) from embryo culture media to detect aneuploid embryos in human in vitro fertilization (IVF) clinics. The cfDNA is released from the embryo during cell division or cell death, suggesting that cfDNA may be a viable resource for sequence analysis. Moreover, cfDNA collection is not invasive to the embryo and does not require special tools or expertise. We hypothesized that selection of appropriate edited embryos could be performed by analyzing cfDNA for MYO7A editing in embryo culture medium, and that this method would be advantageous for the subsequent generation of genetically modified NHPs. The purpose of this experiment is to determine whether cfDNA can be used to identify the target gene mutation of CRISPR/Cas9 injected embryos. In this study, we were able to obtain and utilize cfDNA to confirm the mutagenesis of MYO7A, but the method will require further optimization to obtain better accuracy before it can replace the TE biopsy approach.

• 동물자원연구
• /
• 제30권4호
• /
• pp.165-171
• /
• 2019

본 연구의 목적은 비육돈의 성이 육질과 맛 특성에 미치는 영향을 구명하는 것으로, 이를 위해 총 90두 삼원교잡 LYD 비육돈(180일령 암퇘지 45두와 거세돈 45두; 각 처리구당 1+, 1, 2등급 15마리씩 배치)의 육질, 기계적 맛 및 관능적 맛을 성별에 따라 조사하였다. 등심근의 pH, 근내지방 함량, 육색 및 전단가는 성별 간 차이가 없었지만(p>0.05), 암퇘지와 거세돈의 육즙감량(1.66% vs 1.21%), 유리수분량(12.19% vs 10.68%) 및 가열감량(23.67% vs 21.04%)에서는 유의적인 차이가 확인되었다(p<0.05). 전자혀 측정치는 거세돈의 신맛의 강도가 암퇘지보다 유의적으로 낮은 반면, 감칠맛과 감칠맛후미의 강도는 높았다(p<0.05). 관능검사에서는 풍미, 다즙성 및 연도에서 거세돈이 높은 점수를 받은 결과, 기호성에서도 거세돈(5.51점)이 암퇘지(4.86점)보다 유의적으로 높은 점수를 얻었다(p<0.05). 이러한 결과로부터, 암퇘지보다 높은 거세돈의 보수력이 감칠맛 강도를 증가시켜 관능적 맛도 좋게 한 것으로 사료되었다.