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Studies on Selenium-fortified Functional Hanwoo-Beef by Utilizing Spent Mushroom Composts I. Studies on the Manufacture of Fermented Feeds by Using Spent Mushroom Composts and Fortification of Organic Selenium (버섯폐배지를 이용한 셀레늄강화 기능성 한우고기 생산에 관한 연구 I. 버섯폐배지 이용 발효사료제조와 유기셀레늄 강화에 관한 연구)

  • Lee, Jang-Hyung;Kim, Wan-Young
    • Journal of Practical Agriculture & Fisheries Research
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    • v.6 no.1
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    • pp.102-115
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    • 2004
  • Main objectives of this study were to increase digestibilities of indigestible ingredients in spent mushroom composts (SMC) consisted of sawdust mainly as well as to fortify conversion of inorganic Se present in SMC to organic Se via fermentable microbial actions. Experimental feeds were designed to contain the increasing level of selenium (0.06ppm, 0.54ppm, 1.26ppm and 1.86ppm) in combination with SMCs of Se-enriched and non-Se mushrooms. Feeds were also fermented using commercial microbial feed additives (Sambae, Ltd., Korea) comprised Saccharomyces, Bacillus, Aspergillus, Streptococcus and Actinomycetes before feeding trial for Hanwoo (Korean native cattle). Those were fermented for 0, 12, 24, and 48 hrs. Initial pH was linearly increased as Se concentration increases or the proportion of SMC of Se-enriched mushroom increased (p<0.0001). pH values of fermented feeds (0.54ppm, 1.26ppm and 1.86ppm) containing SMC of Se-enriched mushroom were not different since 12 hrs of fermentation time and their pH was significantly lowered compared to control group. The increasing level of Se concentration in fermented feeds showed significant differences in organic and inorganic Se contents and proportion of organic Se among treatments. As a SMC proportion of Se-enriched mushrooms in the fermented feed was increased, organic Se proportion was significantly decreased (p<0.0001). The control treatment (0.06ppm) comprising the non-Se SMC only was estimated of the organic Se to be 100% and the treatment groups containing the increasing level of Se were estimated of organic Se to be approximately 70%.

Studies on Selenium-fortified Functional Hanwoo-Beef by Utilizing Spent Mushroom Composts II. Effects of Spent Composts of Se-Enriched Mushrooms as the Dietary Se Source on Selenium Deposition in the Muscular Tissue and Plasma Glutathione Peroxidase Activity in the Finishing Hanwoo Steer (버섯폐배지를 이용한 셀레늄강화 기능성 한우고기 생산에 관한 연구 II. 셀레늄강화 버섯폐배지 첨가가 한우의 근육조직 내 셀레늄 축적과 혈중 glutathione peroxidase(GSH-Px)활성에 미치는 영향)

  • Kim, Wan-Young;Lee, Kee-Jong
    • Journal of Practical Agriculture & Fisheries Research
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    • v.6 no.1
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    • pp.116-135
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    • 2004
  • This study was conducted to investigate effects of spent composts of Se-enriched mushrooms as the dietary Se source on muscular Se deposition and plasma glutathione peroxidase (GSH-Px) activity in the finishing Hanwoo steer. Twenty Hanwoo steers were used in the experiment and they were divided into four groups in a randomized complete block design with five replicates. Treatments were four levels (0.1, 0.3, 0.6 and 0.9ppm as fed basis) of dietary Se from the combination with spent composts of Se-enriched mushrooms and/or Se non-enriched mushrooms, in which each treatment was formulated with corn and corn gluten meal and so forth. Treatment diets were fed to Hanwoo steers for 90 days until the slaughter. Dry matter intakes had no significant differences among treatments and there were no significant differences for performances such as total BW gain and ADG among treatments. The Se concentration in blood was linearly increased with increasing dietary selenium levels and reached a plateau level after 8 weeks (p<0.001). Plasma GSH-Px activities had the similar trends to blood Se concentrations by showing that the increased dietary Se level significantly increased plasma GSH-Px activities of both total and Se-dependent (p<0.001). Muscle Se contents of Se-supplemented groups were linearly increased by 1.35 ~ 1.68 folds compared with the control group (0.1ppm; 0.273㎍/dry g) and especially those of the hind legs for 0.9ppm treatment showed the highest Se content as shown 0.457㎍ per dry gram (p<0.01) corresponding to approximately 70% increase of the control group. Se retention rate in the muscle of dietary Se originated from spent composts of Se-enriched mushrooms was estimated of maximum approximately 30% and dietary Se content showed the significant correlation with plasma GSH-Px activities and muscle Se contents (p<0.01). Accordingly, Se present in spent composts of Se-enriched mushroom as the dietary Se source not only had great bioavailabilities showing higher blood Se concentration and plasma GSH-Px activities, but also increased Se deposition in the muscle for Hanwoo beef cattle.

The location of locoregional recurrence in pathologic T3N0, non-irradiated lower rectal cancer

  • Kim, Mi Sun;Keum, Ki Chang;Rhee, Woo Joong;Kim, Hyunju;Kim, Minji;Choi, Seohee;Nam, Ki Chang;Koom, Woong Sub
    • Radiation Oncology Journal
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    • v.31 no.2
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    • pp.97-103
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    • 2013
  • Purpose: To investigate the patterns of locoregional recurrence of pathologic T3N0 (pT3N0) lower rectal cancer omitting postoperative radiotherapy (RT) and explore the potential of modification of a RT field. Materials and Methods: From Jan 2003 to Nov 2011, 35 patients omitting preoperative or postoperative RT for pT3N0 lower rectal cancer were included. We defined the lower rectal cancer as the tumor with the inferior margin located below the virtual line-a convergent level between rectal wall and levator ani muscle. All patients had radiologic examinations for recurrence evaluation during the follow-up duration. Results: The median follow-up duration was 66.4 months (range, 1.4 to 126.1 months). Eight (22.9%) of the 35 patients had recurrence. Three (8.6%) was local recurrence (LR) only, 3 (8.6%) was distant metastasis (DM) only, and 2 (5.7%) was LR with DM. All LR were located at primary tumor sites. The overall survival rate, LR-free survival rate, and DM-free survival rate at 5 years was 79.8%, 83%, and 87%, respectively. All LR developed from tumors over 5 cm. However, there was no statistical significance (p = 0.065). There was no other risk factor for LR. Conclusion: Even though the patients included in this study had pathologically favorable pT3N0 rectal cancer, LR developed in 14.3% of patients. Most of the LR was located at primary tumor sites prior to surgery. Based on these findings, it might seem reasonable to consider postoperative RT with a smaller radiation field to the primary tumor site rather than the conventional whole pelvic irradiation.

Effect of Nitrogen Sources on Mycelial Growth and Sclerotial Formation of Rhizoctonia solani Causing Rice Sheath Blight (벼 잎집무늬마름병균의 균사생장 및 균핵형성에 미치는 질소원의 효과)

  • Park Kyung Seok;Chung Bong Koo
    • Korean Journal Plant Pathology
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    • v.1 no.1
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    • pp.44-50
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    • 1985
  • Effect of nitrogen sources on mycelial growth and sclerotial formation of Rhizoctonia solani causing rice sheath blight was studied by using sixty isolates from diseased samples of different localities in Chungbuk area. Based on the cultural characters, pathogenicity and sclerotial formation, three isolates of the fungus were selected. The virulence of the three isolates varied based on the percent of seeds germination, seedling blights and length and lesion number of lesions. As nitrogen sources, arginine, alanine, urea, ammonium sulfate and sodium nitrate were more effective for mycelial growth and sclerotial formation, than were cystine, methionine, lysine, histidine, tryptophan and thyrosine. The mycelium of R. so/ani grew well in a lower concentration of urea, sodium nitrate, arginine, ammonium sulfate and lysine, but in a higher concentration of proline, histidine, and alanine. In all nitrogen sources tested, high concentration of nitrogen nutrients greatly stimulated sclerotial formation of the fungus. Arginine, urea, and ammonium sulfate increased virulence of the fungus. All the isolates grown on the basic medium plus proline produced large sclerotia. Mycelial growth of the fungus was not well in the tryptophan-added medium, showing brown discoloration of medium.

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Study on the Midwater Trawl Available in the Korean Waters - II . Horizontal Opening of the Gear - (한국 근해에 있어서의 중층 트로올의 연구 - II . 어구의 수평 전개도 -)

  • 이병기
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.22 no.4
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    • pp.49-55
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    • 1986
  • The authors carried out an experiment to determine the horizontal opening of the mid water trawl gear, which is the same as was used in the foreward experiment concerned to the attitude and opening efficiency of otter board. The trawl net .used in the experiment was designed to have a square sectional four-seamed body with two three-sepenlted wings. Each wing tip was rigged with a net pendant of 70 m long and the fore ends of the pendants were directly jointed to the otter pendant without inserting hand rope, and a front weight of 200 kg weigh was rigged with in front of the lowest wing tip. To determine the opening between otter boards ani between wing tips, two 50 KHz fish finders were set sideways on the otter board and on the port middle wing tip so as to detect the distance froa the finders to the starboard otter board and to the wing tip respectively, and the informations were transmitted to the indicators onboard the ship through water-tight cables. The results obtained can be summarized as follows: 1. The openings between otter borads were 43-45 m in case of the warp 100 m, 53-55 m in the war;> 150 m and 54-59 m in the warp 200 m. It was increased in linear according to the increase of towin; speed in the range of 1. 1-1. 8 mlscc. 2. The determined values of the openings between otter boards were greater than the estimated values. which were calculated by the opening between warps at the gallows of the ship and the length of tt~ warp, assuming that the warps from the top rollers to the otter boards were straight. The rates of the determined values to the estimated values were 1. 23-11. 0 in case of the warp 100 m, 1. 23-1. 12 in the warp 150 m and 1. 23-1. 15 in the warp 200 m respectively. The rates were almost the same at the slow towing speed of 1. 3 mlscc or so, then the higher the speed, the less the rate, and the longer the warp, the greater the rate. 3. The rates of the openings between otter boards to the total length of net pendant and the net (excluded cod end) showed 43-43% in case of the warp 150-200 m. It was a little smaller than th~ standard. which is 50%. in the common bottom trawl. 4. The determined openings between wing tips were 18-19 m in case of the warp 100m, 21-22 m in the warp 150 rn, They showed that the higher the speed, the greater the openings even though its range was no more than 1 m or so. 5. The rates of openings between otter boards to th~ openings betwee:l wing tips were almost invariably 38%. 6. The rates of openigs b2tween otter boards to the lenth of h~ad rope were 60-65% in cas': of th~ warp lOa-150m. It were much larg2r than th~ standard. which is 50%. in the common botto:n trawl.

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A Study on Aesthetic Characteristics and social communication of Korean Independent Animation (한국 독립애니메이션의 미학적 특성과 사회적 소통방식 연구 - '인디애니페스트' 수상작 중심으로 -)

  • Seo, Soo-jung
    • Cartoon and Animation Studies
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    • s.47
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    • pp.125-148
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    • 2017
  • This paper analyzes Korean aesthetic characteristics of Korean independent animation, which is closely related to artistic and social characteristics, by searching Korean independent animation image for the winner of "Indie AniFest", the only independent animation film festival in Korea. And social communication methods. There are three ways in which Korean Independence Animation shows the way of speaking through award-winning works. The first is a method of expressing feelings, impulses, and desires in a formative way, and a direct and sensuous image as in sign language or sound. In the second method of speaking differently with images, in the third method, It makes the voice of the fringe through the real world of the story sound as a story of reality. Animation is a medium that 'originalizes and communicates meaning' as an image, and has implemented and constructed a new way of speaking, which is different from existing social voices. Nevertheless, it is not easy to find a research that approaches animation from the viewpoint of social and political speech through images. Therefore, this paper is necessary for the balanced development of animation. In addition, this study can contribute to re - examining Korean independent animation from an academic point of view and to discover and evaluate fair value from a wider perspective.

Studies on the Control of Summer Depression of Pasture Plants -Effect of Mulching on the Productivity and Chemical Composition of Pasture Plants- (목초(牧草)의 하고성(夏枯性) 방제(防除)에 관(關)한 연구(硏究) -초지피복(草地被覆)이 목초(牧草) 생산성(生産性) 및 영양성분(營養成分)에 미치는 영향(影響)-)

  • Kim, Jong-Woo
    • Korean Journal of Agricultural Science
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    • v.3 no.2
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    • pp.184-191
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    • 1976
  • This experiment was conducted for the control of summer depression of cool-season pasture plant. Orchard grass, tall fescue, red clover, alfalfa, and crabgrass were mulched by barley straw with depths of $100g/m^2$, $200g/m^2$ and $300g/m^2$ for 45 days -from July 1, to August 14-, and the effects on growth, yield and chemical composition were observed. The results obetain were as follow: Temperature decrease of $1.0{\sim}3.04^{\circ}C$ was followed after barley straw mulching on the grassland as compared with the control. The growth of the grasses after mulching was hastened, i. e. plant height was increasd 6.0~45% as compared with the control. Barley straw mulching with $100g/m^2$ supported the increase in yield of the grasses with an average of 32.77%, however, average yield was decreased by 7.75% with $300g/m^2$ mulching, apparently due to the rottening of grasses. Chemical compositions of mulched grasses were varied; contents of crude protein and nitrogen free extract were higher, but crude fats, fibre, and ashes were lower than those of the control.

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Effects of Neonatal Exposure of Di (n-butyl) Phthalate and Flutamide on Male Reproduction in Rats

  • Kim, Tae-Sung;Kim, Hyung-Sik;Shin, Jae-Ho;Lee, Su-Jung;Moon, Hyun-Ju;Kang, Il-Hyun;Kim, In-Young;Seok, Ji-Hyun;Oh, Ji-Young;Han, Soon-Young
    • Proceedings of the Korean Society of Embryo Transfer Conference
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    • 2002.11a
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    • pp.109-109
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    • 2002
  • In recent reports, the multiple reproductive defects such as cryptorchidism, hypospadias, epididymal cysts, low sperm counts, and testicular cancers are increased in humans, and these changes were doubted by the chemicals with estrogenic or antiandrogenic activities in our environment. To compare the effects of neonatal exposure of di (n-butyl) phthalate and flutamide on the development of reproductive organs and to identify the specific mechanisms of these abnormalities related to the male reproducton, Sprague-Dawley neonate male rats were injected subcutaneously during 5-14 days after birth with corn oil (control), flutamide (0.05, 0.1, and 0.5 mg/animal) and DBP (5, 10, and 20 mg/animal). Animals were killed at 31 (immature) and 42 (pubertal) days of age respectively and blood was collected from abdominal aorta for serum testosterone analysis. Testes, epididymides, seminal vesicles, ventral prostate, levator ani plus bulbocavernosus muscle (LABC), cowpers glands and glans penis were weighed. Expression of steroid hormone receptors (AR and ER) was examined in the testes and ventral prostate. At 31 days of age, ventral prostate, seminal vesicles, LABC, and cowpers glands significantly decreased in the flutamide (0.5 mg/animal) and DBP (20 mg/animal), but serum testosterone levels were not changed. Flutamide slightly delayed the testes descent at the high dose (0.5 mg/animal), but DBP did not show any significant effect on the testes descent at all doses. DBP and flutamide decreased the expression of AR protein in the testes but did not affect the expression of ERa and ER protein in the testes. At 42 days of age, ventral prostate, seminal vesicles, and cowpers glands weights were still significantly decreased at the high dose of flutamide (0.5 mg/animal) and DBP (20 mg/animal), but the weights of testes and epididymides were not different. Serum testosterone decreased significantly in DBP treated animals and slightly, not significantly, in flutamide group. While DBP still significantly decreased the expression of AR protein in testis, flutamide recovered from downregulation of AR protein and did not affect the expression of ERa and ER protein in the testes. Based on these results, flutamide and DBP have shown several similar patterns in reproductive abnormalitis, but some marked differences which may be caused by different acting mechanism.

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Studies on the seeding systems and varieties adapted to mechanization in barley and wheat culture (맥류 기계화 적응재배 양식과 적응품종의 생태에 관한 연구)

  • Jae-Young Cho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.8 no.1
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    • pp.17-29
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    • 1970
  • To research the seeding systems and the varieties adapted to mechanization in barley and wheat culture in Korea, the studies were carried out from October, 1969 to June, 1970 at Suwon. In these studies, six kinds of seeding systems of drilling and dibbling that seems to adapted to mechanization were tested as compared with the customary seeding system, furrowing moderately or widely, using three varieties of barley, Suwon #18, Barsoy and Buhufng, and three varieties of wheat, Wonkwang, Yucseung #3 and Yeungkwang, under the two fertilizer levels of standard and double. The summarize results gained were as follows; 1. Buhung seemed to be the most suitable variety in barley as it yielded the most and matured early and grew the safest. Barsoy suffered from winter injury. 2. Yeungkwang seemed to be the most suitable variety in wheat as it yielded the most and produced the largest grain ani grew the safest, however it matured late. Wonkwang suffered heavily from lodging injury. 3. More yield were produced and there were no increase of cultural dangers except lodging in double fertilizer level. 4. Seeding system of drilling and dibbling seems to be able to put in practice safely under the double fertilizer level when lodging resistant varieties are selected. 5. Rate of yields increase in comparison with the customary seeding system of moderate furrowing were 16 percent in drilling of 20 centimeter spacing, 13 percent in wide furrowing and 12 percent in dibbling of 20 ${\times}$ 12 centimeter spacing in barley and also 9 percent in wide furrowing, 8 percent in dibbling of 20 ${\times}$ 12 centimeter spacing and 7 percent in drilling of 20 centimeter spacing in wheat. 6. The most important cause of above yields increase seems to be the increase of spike number per unit area. 7. Yields increase by drilling as compared with furrowing was not caused by higher rate of seeding. 8. The plants matured a bit earlier by drilling and dibbling. 9. Better stands of seedling were fount in dibbling due to the promotion of germination by tramping. 10. An increase of yields will be expected by decreasing the spacing in drilling and dibbling.

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The Effects of Flutamide and Diethylstilbestrol Exposure on the Reproductive Organs and Thyroid of Male Rats by the Rodent 20-day Thyroid/Pubertal Assay

  • Moon, Hyun-Ju;Shin, Jae-Ho;Kim, Hyung-Sik;Lee, Su-Jung;Kang, Il-Hyun;Kim, Tae-Sung;Seok, Ji-Hyun;Kim, In-Young;Han, Soon-Young
    • Proceedings of the Korea Society of Environmental Toocicology Conference
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    • 2002.10a
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    • pp.168-168
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    • 2002
  • To establish a test protocol for the rodent 20-day thyroid/pubertal assay, flutamide and diethylstilbestrol (DES) were administered to intact male Sprague-Dawley rats from postnatal day 33 for 20 days. Flutamide (1, 5, and 25 mg/kg/day) or DES (10, 20, and 40 ug/kg/day) was given once daily by oral gavage to immature male rats. Prepuce separation was significantly delayed in flutamide group and in DES group. One day after the last dose, the rats were killed and pituitary, thyroid, and reproductive organs were removed and weighed. Flutamide treatment resulted in a significant reduction in the weights of epididymides, ventral prostate, seminal vesicles plus coagulating glands and fluid (SVCGF), levator ani. bulbocarvenus muscles (LABC), Cowper's glands, and glans penis. The weight of adrenal glands decreased at % mg/kg/day, while testes and any other organ weights were unaffected. No microscopic changes were observed in the thyroid glands. Serum levels of testosterone wert significantly increased in the flutamide-treated groups and serum levels of estradiol were also increased. A significant reduction in the weights of testes, epididymides, ventral prostate, SVCGF, LABC, Cowpers glands, and glans penis of DES treated group. Serum testosterone and LH decreased significantly in DES group. Decrease of estradiol was observed, but not significant. These results indicate that flutamide and DES delay puberty in the male rat and its mode of action appears to be via altered secretion of steroids, which subsequently affect the development of the reproductive tract. (Supported by the grant from NITR/Korea FDA for Endocrine Disrupter Research.)

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