• Journal of the Korean Society of Food Science and Nutrition
• /
• v.43 no.9
• /
• pp.1363-1368
• /
• 2014

The physiological activities of cultivated Korean blue mussel (Mytilus edulis) and New Zealand green-lipped mussel (Perna canaliculus) were analyzed and compared. Both hot water extracts of blue mussel flesh (BMF) and green-lipped mussel flesh (GMF) showed increased activities of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH). BMF showed increased ADH and slightly decreased ALDH activities compared to GMF. 1,1-Diphenyl-2-picrylhydrazyl radical scavenging activity of BMF was higher than that of GMF at the same concentration. BMF and GMF showed similar inhibitory activity against angiotensin converting enzyme at a concentration of 30 mg/mL. These results suggest that cultivated Korean blue mussel has similar physiological activity with New Zealand green-lipped mussel.

• Microbiology and Biotechnology Letters
• /
• v.36 no.4
• /
• pp.291-298
• /
• 2008

The cardiovascular angiotensin I-converting enzyme inhibitory activity, fibrinolytic activity and bbb-secretase inhibitory activity of four kinds of red wine were investigated during fermentation and post-fermentation. After 10 days of fermentation, the antihypertensive angiotensin I-converting enzyme (ACE) inhibitory activities of all the red wines ranged from 38.6% to 58.8%. However, the ACE inhibitory activities increased with the prolongation of the post-fermentation period; moreover, in the Vitis hybrid red wine, the ACE inhibitory activity reached its highest value, 76.9%, after 120 days of post-fermentation. During the fermentation and post-fermentation of all the red wines, fibrinolytic activity was weak or not detected. After 10 days of fermentation, Vitis labrusca B red wine exhibited the greatest antidementia $\beta$-secretase inhibitory activity of 54.8%, though $\beta$-secretase inhibitory activity decreased significantly to less than 10% during 120 days of post-fermentation. In conclusion, we obtained a highly valuable Vitis hybrid red wine that was fermented for 10 days at $25^{\circ}C$ with Vitis hybrid and S. cerevisiae K-7 and then post-fermentation for 120 days at $4^{\circ}C$.

• Proceedings of the Korean Society of Fisheries Technology Conference
• /
• 2001.10a
• /
• pp.97-98
• /
• 2001

순환기계 질병의 원인이 되는 동시에 뇌출혈, 심장병 및 신장병 등과 합병증으로 나타날 경우 치사율이 매우 높은 만성 퇴행성 질환인 고혈압의 90％ 이상을 차지하는 본태성 고혈압은 정상적인 혈압을 유지하는 기구들이 천천히 붕괴되어 진행되는 질병이다(Frohlich, 1982). 이러한 본태성 고혈압의 원인 중에서 reninㆍangiotensin계가 혈압조절에 매우 중요한 역할을 한다고 알려지고 있다(Saxena, 1992). 즉, angiotensinogen이 renin의 분해를 받아서 angiotensin I을 생성하는데, 이는 angiotensin converting enzyme(ACE)에 의하여 COOH 말단의 dipeptide가 절단되어 강력한 혈관수축작용을 하는 angiotensin II를 생성한다. (중략)

• Preventive Nutrition and Food Science
• /
• v.3 no.4
• /
• pp.379-381
• /
• 1998

An angiotensin converting enzyme (ACE) inhibitor was isolated and partially purified from bovine blood plasma. Bovine blood plasma was obtained after removing blood cells by centrifugation, followed by the addition of anticoagulant to whole bovine blood. To precipitate plasma proteins, bovine blood plasma was treated with 4% trichloroacetic acid (TCA) as a final concentration .An ACE inhibitor was isolated from TCA supernatnat, using ultrafiltration, gel permeation chormatography, and reverse-phase high pressure liquid chromatogrpahy. The ACE inhibitor purified from TCA supernatant had IC50 values of 9.4$\mu$M.

• YAKHAK HOEJI
• /
• v.31 no.1
• /
• pp.1-9
• /
• 1987

The preparation of substituted benzoylamino acids and mercaptoacylariaino acids as inhibitors of angiotensin converting enzyme is described. The bulky phenyl ring directly attacted to an amino acid as of benzoylamino acid seemed unsuitable for the inhibitory activities. Among the mercaptosuccinylamino acids, /sub L/-proline was more favorable than /sub L/-phenylalanine while S-acetylation was unfavorable.

• Proceedings of the Korean Society of Applied Pharmacology
• /
• 1994.04a
• /
• pp.182-182
• /
• 1994

Angiotensin converting enzyme (ACE)을 비가역적으로 불활성화시킴으로써 오랫동안 작용할 수 있는 고혈압치료제로서의 ACE억제제를 개발하기 위하여pseudomechanism-based inhibition이라는 새로운 억제기전을 가질 것으로 추정되는 아래 그림과 같은 기본 분자구조를 갖는 epoxide 유도체들을 합성하여 in vitro에서 ACE활성 억제효과를, HPLC법을 이용하여 측정하였다. 그 결과 합성되어진 epoxide 유도체들은, epoxide group대신에 sulfhydryl 또는 carboxyl group으로 치환되어져 있는 기존의 ACE 억제제들보다도 효능이 현저히 저하됨으로써, ACE의 $Zn^{2+}$ binding site와는 배위결합력이 미약하다는 것을 의미하여 준다. 또한 유도체들의 phenylring에 chloride, hydroxyl, nitro group과 같은 polar group 의 도입으로 말미암아 ACE 억제효과가 저하됨으로써 이 부위에서의 hydrophobic interaction이 ACE를 억제하는데 중요하다는 것을 시사해 주며 이외에도 이미 알려진 바와같이 carbonyl carbon과 인접한 carbon atom에 methyl group의 도입이 억제효과에 중요한 역활을 하였다. 따라서 향후에는 ACE의 $Zn^{2+}$ binding site와 강력한 배위결합을 하는 carboxyl group을 도입하고 epoxide의 위치를 변경시키며 또한 hydrophobic interaction하는 부위의 구조를 변화시켜 보다 효능이 우수한 새로운 기전의 ACE억제제를 개발해 나가고자 한다.

• Korean Journal of Pharmacognosy
• /
• v.12 no.1
• /
• pp.51-54
• /
• 1981

Twenty-seven medicinal plants were selected on the basis of folkloric reputation for the treatment of hypertension or related deseases. Two solvent fractions were prepared from methanol extract of each plant and tested for their effects on angiotensin converting enzyme (ACE) activities. Six solvent fractions showed more than 50% inhibition and four showed $40{\sim}50%$ inhibition at the conditions tested.

• Fisheries and Aquatic Sciences
• /
• v.12 no.2
• /
• pp.79-85
• /
• 2009

Gelatin hydrolysates with a high inhibitory activity against angiotensin I-converting enzyme (ACE) were fractionated from Alaska pollock surimi refiner discharge. The ACE-inhibitory activity, expressed as $IC_{50}$ (mg/mL), was highest (0.49 mg/mL) in gelatin hydrolysates formed by sequential 2-hr treatments of Pronase and Flavourzyme. After fractionation through four different membrane filters with molecular weight cut-offs of 3, 5, 10, and 30 kDa, the highest ACE-inhibitory activity (0.21 mg/mL) was observed with the 3-kDa filtrate.

• Archives of Pharmacal Research
• /
• v.21 no.2
• /
• pp.168-173
• /
• 1998

The epoxyalkanoyl derivatives were designed and synthesized as ACE inhibitors. Coupling of unsaturated carboxylic acids with amino acids and following epoxidation with dimethyldioxirane gave the epoxyalkanoyls with high yield. The inhibitory activity of synthesized compounds on angiotensin converting enzyme was $IC_{50}$ values of 0.06~5.5 ${\mu}M$.

• Preventive Nutrition and Food Science
• /
• v.9 no.1
• /
• pp.95-97
• /
• 2004

An angiotensin converting enzyme (ACE) inhibitory substance was isolated and purified from Lycium chinense Miller. A crude water extract of Lycium chinense Miller was prepared by adding it to water shaking at $25^{\circ}C$ for 1 hr, followed by centrifugation at 8000 ${\times}$ g for 30 min. The crude extract was then filtered using YM-3 and YM-1 membranes. An ACE inhibitor was isolated using consecutive chromatographic methods including: ion exchange chromatography, gel permeation chromatography, and FPLC. The inhibitor was identified to have a molecular mass of 862 daltons by mass spectrometry.